Cryo-EM structure of native RC-LH complex from Roseiflexus castenholzii at 10,000 lux
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 8hju
Q-score: 0.603
Xin J, Shi Y, Zhang X, Yuan X, Xin Y, He H, Shen J, Blankenship RE, Xu X
eLife (2023) 12 [ DOI: doi:10.7554/eLife.88951 Pubmed: 37737710 ]
- Bacteriochlorophyll a (911 Da, Ligand)
- Subunit x (3 kDa, Protein from Roseiflexus castenholzii DSM 13941)
- Fe (iii) ion (55 Da, Ligand)
- Alpha subunit of light-harvesting 1 (4 kDa, Protein from Roseiflexus castenholzii DSM 13941)
- Multiheme_cytc domain-containing protein (34 kDa, Protein from Roseiflexus castenholzii DSM 13941)
- Reaction center protein m chain (35 kDa, Protein from Roseiflexus castenholzii DSM 13941)
- The native rc-lh complex (Complex from Roseiflexus castenholzii DSM 13941)
- Protoporphyrin ix containing fe (616 Da, Ligand)
- Beta,psi-caroten-4-one (550 Da, Ligand)
- 2-methyl-3-[(2e,6e,10e,14e,18e,22e,26e,30e,34e,38e)-3,7,11,15,19,23,27,31,35,39,43-undecamethyltetratetraconta-2,6,10,1 4,18,22,26,30,34,38,42-undecaen-1-yl]naphthalene-1,4-dione (921 Da, Ligand)
- Beta subunit of light-harvesting 1 (6 kDa, Protein from Roseiflexus castenholzii DSM 13941)
- Diacyl glycerol (625 Da, Ligand)
- Bacteriopheophytin a (889 Da, Ligand)
- Subunit y (4 kDa, Protein from Roseiflexus castenholzii DSM 13941)
- Subunit z (6 kDa, Protein from Roseiflexus castenholzii DSM 13941)
- Reaction center protein l chain (34 kDa, Protein from Roseiflexus castenholzii DSM 13941)
- (1r)-2-{[{[(2s)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(palmitoyloxy)methyl]ethyl (11e)-octadec-11-enoate (749 Da, Ligand)
Cryo-EM structure of carotenoid-depleted RC-LH complex from Roseiflexus castenholzii at 10,000 lux
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 8hjv
Q-score: 0.478
Xin J, Shi Y, Zhang X, Yuan X, Xin Y, He H, Shen J, Blankenship RE, Xu X
eLife (2023) 12 [ DOI: doi:10.7554/eLife.88951 Pubmed: 37737710 ]
- Bacteriochlorophyll a (911 Da, Ligand)
- The carotenoid-depleted rc-lh complex (Complex from Roseiflexus castenholzii DSM 13941)
- Fe (iii) ion (55 Da, Ligand)
- Alpha subunit of light-harvesting 1 (4 kDa, Protein from Roseiflexus castenholzii DSM 13941)
- Multiheme_cytc domain-containing protein (34 kDa, Protein from Roseiflexus castenholzii DSM 13941)
- Reaction center protein m chain (35 kDa, Protein from Roseiflexus castenholzii DSM 13941)
- Protoporphyrin ix containing fe (616 Da, Ligand)
- Beta,psi-caroten-4-one (550 Da, Ligand)
- 2-methyl-3-[(2e,6e,10e,14e,18e,22e,26e,30e,34e,38e)-3,7,11,15,19,23,27,31,35,39,43-undecamethyltetratetraconta-2,6,10,1 4,18,22,26,30,34,38,42-undecaen-1-yl]naphthalene-1,4-dione (921 Da, Ligand)
- Beta subunit of light-harvesting 1 (6 kDa, Protein from Roseiflexus castenholzii DSM 13941)
- Bacteriopheophytin a (889 Da, Ligand)
- Subunit y (4 kDa, Protein from Roseiflexus castenholzii DSM 13941)
- Subunit z (6 kDa, Protein from Roseiflexus castenholzii DSM 13941)
- Reaction center protein l chain (34 kDa, Protein from Roseiflexus castenholzii DSM 13941)
- (1r)-2-{[{[(2s)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(palmitoyloxy)methyl]ethyl (11e)-octadec-11-enoate (749 Da, Ligand)
Cryo-EM structure of native RC-LH complex from Roseiflexus castenholzii at 100lux
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 8j5o
Q-score: 0.606
Xin J, Shi Y, Zhang X, Yuan X, Xin Y, He H, Shen J, Blankenship RE, Xu X
eLife (2023) 12 [ DOI: doi:10.7554/eLife.88951 Pubmed: 37737710 ]
- Bacteriochlorophyll a (911 Da, Ligand)
- Subunit z (6 kDa, Protein from Roseiflexus castenholzii DSM 13941)
- Fe (iii) ion (55 Da, Ligand)
- Alpha subunit of light-harvesting 1 (4 kDa, Protein from Roseiflexus castenholzii DSM 13941)
- Multiheme_cytc domain-containing protein (34 kDa, Protein from Roseiflexus castenholzii DSM 13941)
- Reaction center protein m chain (35 kDa, Protein from Roseiflexus castenholzii DSM 13941)
- Protoporphyrin ix containing fe (616 Da, Ligand)
- Subunit x (3 kDa, Protein from Roseiflexus castenholzii DSM 13941)
- The native rc-lh complex at 100lux (Complex from Roseiflexus castenholzii DSM 13941)
- 2-methyl-3-[(2e,6e,10e,14e,18e,22e,26e,30e,34e,38e)-3,7,11,15,19,23,27,31,35,39,43-undecamethyltetratetraconta-2,6,10,1 4,18,22,26,30,34,38,42-undecaen-1-yl]naphthalene-1,4-dione (921 Da, Ligand)
- Beta subunit of light-harvesting 1 (6 kDa, Protein from Roseiflexus castenholzii DSM 13941)
- Subunit y (4 kDa, Protein from Roseiflexus castenholzii DSM 13941)
- Bacteriopheophytin a (889 Da, Ligand)
- Beta,psi-caroten-4-one (550 Da, Ligand)
- (1r)-2-{[{[(2s)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(palmitoyloxy)methyl]ethyl (11e)-octadec-11-enoate (749 Da, Ligand)
- Reaction center protein l chain (34 kDa, Protein from Roseiflexus castenholzii DSM 13941)
Cryo-EM structure of native RC-LH complex from Roseiflexus castenholzii at 2,000lux
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 8j5p
Q-score: 0.562
Xin J, Shi Y, Zhang X, Yuan X, Xin Y, He H, Shen J, Blankenship RE, Xu X
eLife (2023) 12 [ DOI: doi:10.7554/eLife.88951 Pubmed: 37737710 ]
- Bacteriochlorophyll a (911 Da, Ligand)
- Bacteriopheophytin b (887 Da, Ligand)
- Subunit z (6 kDa, Protein from Roseiflexus castenholzii DSM 13941)
- Fe (iii) ion (55 Da, Ligand)
- Alpha subunit of light-harvesting 1 (4 kDa, Protein from Roseiflexus castenholzii DSM 13941)
- Multiheme_cytc domain-containing protein (34 kDa, Protein from Roseiflexus castenholzii DSM 13941)
- Reaction center protein m chain (35 kDa, Protein from Roseiflexus castenholzii DSM 13941)
- Protoporphyrin ix containing fe (616 Da, Ligand)
- Subunit x (3 kDa, Protein from Roseiflexus castenholzii DSM 13941)
- The native rc-lh complex at 2,000lux (Complex from Roseiflexus castenholzii DSM 13941)
- 2-methyl-3-[(2e,6e,10e,14e,18e,22e,26e,30e,34e,38e)-3,7,11,15,19,23,27,31,35,39,43-undecamethyltetratetraconta-2,6,10,1 4,18,22,26,30,34,38,42-undecaen-1-yl]naphthalene-1,4-dione (921 Da, Ligand)
- Beta subunit of light-harvesting 1 (6 kDa, Protein from Roseiflexus castenholzii DSM 13941)
- Subunit y (4 kDa, Protein from Roseiflexus castenholzii DSM 13941)
- Beta,psi-caroten-4-one (550 Da, Ligand)
- (1r)-2-{[{[(2s)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(palmitoyloxy)methyl]ethyl (11e)-octadec-11-enoate (749 Da, Ligand)
- Reaction center protein l chain (34 kDa, Protein from Roseiflexus castenholzii DSM 13941)