Cryo-EM structure of BG505 SOSIP trimer purified via Galanthus nivalis lectin chromatography
Parthasarathy D, Pothula KR, Ratnapriya S, Cervera Benet H, Parsons R, Huang X, Sammour S, Janowska K, Harris M, Sodroski J, Acharya P, Herschhorn A
Nat Commun (2024) 15 pp. 7334-7334 [ Pubmed: 39187497 DOI: doi:10.1038/s41467-024-51656-4 ]
HIV Envelope trimer BG505 SOSIP.664 in complex with wild type CH103 antibody
Liu Q, Parsons R, Wiehe K, Edwards RJ, Saunders KO, Zhang P, Miao H, Williams W, Huang X, Janowska K, Mansouri K, Acharya P, Haynes BF, Lusso P
To Be Published
BG505 SOSIP reconstructed from a designed tetrahedral nanoparticle, BG505 SOSIP-T33_dn2
Resolution: 4.5 Å
EM Method: Single-particle
Fitted PDBs: 6vl5
Q-score: 0.333
Martin JT, Cottrell CA, Antanasijevic A, Carnathan DG, Cossette BJ, Enemuo CA, Gebru EH, Choe Y, Viviano F, Fischinger S, Tokatlian T, Cirelli KM, Ueda G, Copps J, Schiffner T, Menis S, Alter G, Schief WR, Crotty S, King NP, Baker D, Silvestri G, Ward AB, Irvine DJ
NPJ Vaccines (2020) 5 pp. 72-72 [ Pubmed: 32802411 DOI: doi:10.1038/s41541-020-00223-1 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Bg505 sosip trimer reconstructed from a designed tetrahedral nanoparticle bg505 sosip-t33_dn2 (Complex from synthetic construct)
- Bg505 sosip.v5.2(7s) - gp120 (56 kDa, Protein from synthetic construct)
- Bg505 sosip.v5.2(7s) - gp41 (18 kDa, Protein from synthetic construct)
De novo designed icosahedral nanoparticle I53_dn5 presenting BG505-SOSIP, Cryo-EM Map
Antanasijevic A, Ueda G, Brouwer PJM, Copps J, Huang D, Allen JD, Cottrell CA, Yasmeen A, Sewall LM, Bontjer I, Ketas TJ, Turner HL, Berndsen ZT, Montefiori DC, Klasse PJ, Crispin M, Nemazee D, Moore JP, Sanders RW, King NP, Baker D, Ward AB
PLoS Pathog (2020) 16 pp. e1008665-e1008665 [ Pubmed: 32780770 DOI: doi:10.1371/journal.ppat.1008665 ]
- De novo designed icosahedral nanoparticle i53_dn5 displaying 20 copies of bg505-sosip trimer on the surface. nanoparticle consists of 60 copies of both subunits (bg505-sosip-i53_dn5b and i53_dn5a) (Complex from synthetic construct)
- Bg505-sosip-i53_dn5b (Protein from synthetic construct)
- I53_dn5a (Protein from synthetic construct)
Resolution: 4.25 Å
EM Method: Single-particle
Fitted PDBs: 6vfk
Q-score: 0.316
Antanasijevic A, Ueda G, Brouwer PJM, Copps J, Huang D, Allen JD, Cottrell CA, Yasmeen A, Sewall LM, Bontjer I, Ketas TJ, Turner HL, Berndsen ZT, Montefiori DC, Klasse PJ, Crispin M, Nemazee D, Moore JP, Sanders RW, King NP, Baker D, Ward AB
PLoS Pathog (2020) 16 pp. e1008665-e1008665 [ Pubmed: 32780770 DOI: doi:10.1371/journal.ppat.1008665 ]
- T33_dn10b (31 kDa, Protein from synthetic construct)
- De novo designed tetrahedral nanoparticle t33_dn10 displaying 4 copies of bg505-sosip trimer on the surface. nanoparticle consists of 12 copies of both subunits (bg505-sosip-t33_dn10a and t33_dn10b) (Complex from synthetic construct)
- Bg505-sosip-t33_dn10a (88 kDa, Protein from synthetic construct)
Nogal B, Bianchi M, Cottrell CA, Kirchdoerfer RN, Sewall LM, Turner HL, Zhao F, Sok D, Burton DR, Hangartner L, Ward AB
Cell Rep (2020) 30 pp. 3755-3765.e7 [ DOI: doi:10.1016/j.celrep.2020.02.061 Pubmed: 32187547 ]
Cryo-EM structure of a BG505 Env-sCD4-17b-8ANC195 complex
Resolution: 8.9 Å
EM Method: Single-particle
Fitted PDBs: 5thr
Q-score: 0.12
Wang H, Cohen AA, Galimidi RP, Gristick HB, Jensen GJ, Bjorkman PJ
PNAS (2016) 113 pp. E7151-E7158 [ Pubmed: 27799557 DOI: doi:10.1073/pnas.1615939113 ]
- 8anc195 g52k5 vl domain (23 kDa, Protein from Homo sapiens)
- T-cell surface glycoprotein cd4 (21 kDa, Protein from Homo sapiens)
- Bg505 sosip trimer (Complex from Human immunodeficiency virus 1)
- 17b fab vh domain (24 kDa, Protein from Homo sapiens)
- 17b fab vl domain (23 kDa, Protein from Homo sapiens)
- Bg505 sosip gp41 (17 kDa, Protein from Human immunodeficiency virus 1)
- 8anc195 g52k5 vh domain (26 kDa, Protein from Homo sapiens)
- Bg505 sosip gp120 (54 kDa, Protein from Human immunodeficiency virus 1)
- 17b fab (Complex from Homo sapiens)
- 8anc195 g52k5 fab (Complex from Homo sapiens)
- Bg505 sosip-scd4-17b-8anc195 complex (50 kDa, Complex)
- Cd4 d1-d2 domain (Cellular component from Homo sapiens)