cryo-EM structure of sea urchin sperm doublet microtubule (48-nm periodicity)
Leung MR, Zeng J, Wang X, Roelofs MC, Huang W, Zenezini Chiozzi R, Hevler JF, Heck AJR, Dutcher SK, Brown A, Zhang R, Zeev-Ben-Mordehai T
Cell (2023) 186 pp. 2880- [ Pubmed: 37327785 DOI: doi:10.1016/j.cell.2023.05.026 ]
- Tex33 (40 kDa, Protein from Strongylocentrotus purpuratus)
- Tex43 (14 kDa, Protein from Strongylocentrotus purpuratus)
- Coiled-coil domain-containing protein 105 (49 kDa, Protein from Strongylocentrotus purpuratus)
- Cfap90(c5orf49) (19 kDa, Protein from Strongylocentrotus purpuratus)
- Rib35 (34 kDa, Protein from Strongylocentrotus purpuratus)
- Spata45 (9 kDa, Protein from Strongylocentrotus purpuratus)
- Cilia- and flagella-associated protein 97 (27 kDa, Protein from Strongylocentrotus purpuratus)
- Protein phosphatase 1 regulatory subunit 32 (52 kDa, Protein from Strongylocentrotus purpuratus)
- Flagellar protofilament ribbon protein rib74 (74 kDa, Protein from Strongylocentrotus purpuratus)
- C4orf45 (32 kDa, Protein from Strongylocentrotus purpuratus)
- Tex49 (17 kDa, Protein from Strongylocentrotus purpuratus)
- Cilia- and flagella-associated protein 45 (64 kDa, Protein from Strongylocentrotus purpuratus)
- Nucleoside diphosphate kinase (42 kDa, Protein from Strongylocentrotus purpuratus)
- Spata48 (23 kDa, Protein from Strongylocentrotus purpuratus)
- Outer dense fiber protein 3 (27 kDa, Protein from Strongylocentrotus purpuratus)
- Helo_like_n(loc577943) (34 kDa, Protein from Strongylocentrotus purpuratus)
- Magnesium ion (24 Da, Ligand)
- Cilia- and flagella-associated protein 77 (27 kDa, Protein from Strongylocentrotus purpuratus)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Cfap276 (19 kDa, Protein from Strongylocentrotus purpuratus)
- Tubulin alpha chain (50 kDa, Protein from Strongylocentrotus purpuratus)
- Flagellar flij protein (14 kDa, Protein from Strongylocentrotus purpuratus)
- Cfap144(fam183a) (16 kDa, Protein from Strongylocentrotus purpuratus)
- Meiosis-specific nuclear structural protein 1 (61 kDa, Protein from Strongylocentrotus purpuratus)
- Meiosis-specific nuclear structural protein 1 (17 kDa, Protein from Strongylocentrotus purpuratus)
- Cilia- and flagella-associated protein 52 (70 kDa, Protein from Strongylocentrotus purpuratus)
- Cilia- and flagella-associated protein 126 (15 kDa, Protein from Strongylocentrotus purpuratus)
- Cfap68(upf0686, c11orf1) (17 kDa, Protein from Strongylocentrotus purpuratus)
- Rib43a-like with coiled-coils protein 2 (45 kDa, Protein from Strongylocentrotus purpuratus)
- Cfa20 domain-containing protein (22 kDa, Protein from Strongylocentrotus purpuratus)
- Outer dense fiber protein 3 (30 kDa, Protein from Strongylocentrotus purpuratus)
- Fam166c (23 kDa, Protein from Strongylocentrotus purpuratus)
- Tektin (49 kDa, Protein from Strongylocentrotus purpuratus)
- C20orf85 (23 kDa, Protein from Strongylocentrotus purpuratus)
- Tex36(cfap95, c9orf135) (25 kDa, Protein from Strongylocentrotus purpuratus)
- Tex26(loc100888047) (19 kDa, Protein from Strongylocentrotus purpuratus)
- Cfap96(c4orf47) (33 kDa, Protein from Strongylocentrotus purpuratus)
- Pacrg (26 kDa, Protein from Strongylocentrotus purpuratus)
- Tektin (46 kDa, Protein from Strongylocentrotus purpuratus)
- Cilia- and flagella-associated protein 161 (32 kDa, Protein from Strongylocentrotus purpuratus)
- Testicular haploid expressed gene protein-like (30 kDa, Protein from Strongylocentrotus purpuratus)
- Coiled-coil domain-containing protein 81 (79 kDa, Protein from Strongylocentrotus purpuratus)
- Atp6v1fnb (21 kDa, Protein from Strongylocentrotus purpuratus)
- Sea urchin sperm doublet microtubule (Cellular component from Strongylocentrotus purpuratus)
- Ef-hand domain-containing family member c2 (85 kDa, Protein from Strongylocentrotus purpuratus)
- Tex49_homologue(loc580808) (20 kDa, Protein from Strongylocentrotus purpuratus)
- Protein fam166b (48 kDa, Protein from Strongylocentrotus purpuratus)
- Pierce1 (13 kDa, Protein from Strongylocentrotus purpuratus)
- Cfap107 (27 kDa, Protein from Strongylocentrotus purpuratus)
- Trichohyalin-plectin-homology domain-containing protein (62 kDa, Protein from Strongylocentrotus purpuratus)
- Saxo3(loc115918676) (37 kDa, Protein from Strongylocentrotus purpuratus)
- Tepp protein (21 kDa, Protein from Strongylocentrotus purpuratus)
- Ef-hand domain-containing protein (66 kDa, Protein from Strongylocentrotus purpuratus)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Enkurin domain-containing protein (30 kDa, Protein from Strongylocentrotus purpuratus)
- Sperm-associated antigen 8 (23 kDa, Protein from Strongylocentrotus purpuratus)
- Tektin (53 kDa, Protein from Strongylocentrotus purpuratus)
- Trichohyalin-plectin-homology domain-containing protein (65 kDa, Protein from Strongylocentrotus purpuratus)
- Tubulin beta chain (50 kDa, Protein from Strongylocentrotus purpuratus)
cryoEM reconstruction of the terminal C9s and chaperone in 3C9-sMAC
Menny A, Lukassen MV, Couves EC, Franc V, Heck AJR, Bubeck D
Nat Commun (2021) 12 pp. 6086-6086 [ DOI: doi:10.1038/s41467-021-26366-w Pubmed: 34667172 ]
- 3c9-smac (Complex from Homo sapiens)
cryoEM reconstruction of the terminal C9s in 2C9-sMAC
Menny A, Lukassen MV, Couves EC, Franc V, Heck AJR, Bubeck D
Nat Commun (2021) 12 pp. 6086-6086 [ DOI: doi:10.1038/s41467-021-26366-w Pubmed: 34667172 ]
- 2c9-smac (Complex from Homo sapiens)
cryoEM reconstruction of C5b and C7 C-ter in 2C9-sMAC
Menny A, Lukassen MV, Couves EC, Franc V, Heck AJR, Bubeck D
Nat Commun (2021) 12 pp. 6086-6086 [ DOI: doi:10.1038/s41467-021-26366-w Pubmed: 34667172 ]
- 2c9-smac (Complex from Homo sapiens)
cryoEM reconstruction of 1C9-sMAC
Menny A, Lukassen MV, Couves EC, Franc V, Heck AJR, Bubeck D
Nat Commun (2021) 12 pp. 6086-6086 [ DOI: doi:10.1038/s41467-021-26366-w Pubmed: 34667172 ]
- 1c9-smac (1 MDa, Complex from Homo sapiens)
- Complement protein c7 (Protein from Homo sapiens)
- Complement protein c8 beta (Protein from Homo sapiens)
- Complement protein c8 gamma (Protein from Homo sapiens)
- Complement protein c8 alpha (Protein from Homo sapiens)
- Complement protein c9 (Protein from Homo sapiens)
- Complement protein c5b (Protein from Homo sapiens)
- Complement protein c6 (Protein from Homo sapiens)
Resolution: 3.54 Å
EM Method: Single-particle
Fitted PDBs: 7nyc
Q-score: 0.226
Menny A, Lukassen MV, Couves EC, Franc V, Heck AJR, Bubeck D
Nat Commun (2021) 12 pp. 6086-6086 [ DOI: doi:10.1038/s41467-021-26366-w Pubmed: 34667172 ]
- 3c9-smac (1 MDa, Complex from Homo sapiens)
- Alpha-d-mannopyranose (180 Da, Ligand)
- Complement component c8 alpha chain (61 kDa, Protein from Homo sapiens)
- Complement c5 (186 kDa, Protein from Homo sapiens)
- Complement component c8 gamma chain (20 kDa, Protein from Homo sapiens)
- Alpha-l-fucopyranose (164 Da, Ligand)
- Complement component c6 (102 kDa, Protein from Homo sapiens)
- Complement component c8 beta chain (61 kDa, Protein from Homo sapiens)
- Calcium ion (40 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Complement component c9 (61 kDa, Protein from Homo sapiens)
- Complement component c7 (91 kDa, Protein from Homo sapiens)
Resolution: 3.27 Å
EM Method: Single-particle
Fitted PDBs: 7nyd
Q-score: 0.335
Menny A, Lukassen MV, Couves EC, Franc V, Heck AJR, Bubeck D
Nat Commun (2021) 12 pp. 6086-6086 [ DOI: doi:10.1038/s41467-021-26366-w Pubmed: 34667172 ]
- Alpha-d-mannopyranose (180 Da, Ligand)
- Complement component c8 alpha chain (61 kDa, Protein from Homo sapiens)
- Complement c5 (186 kDa, Protein from Homo sapiens)
- Complement component c8 gamma chain (20 kDa, Protein from Homo sapiens)
- Alpha-l-fucopyranose (164 Da, Ligand)
- Complement component c6 (102 kDa, Protein from Homo sapiens)
- 2c9-smac (1 MDa, Complex from Homo sapiens)
- Complement component c8 beta chain (61 kDa, Protein from Homo sapiens)
- Calcium ion (40 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Complement component c9 (61 kDa, Protein from Homo sapiens)
- Complement component c7 (91 kDa, Protein from Homo sapiens)