yeast 80S ribosome in complex with the Not5 subunit of the CCR4-NOT complex
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 6tb3
Q-score: 0.543
Buschauer R, Matsuo Y, Sugiyama T, Chen YH, Alhusaini N, Sweet T, Ikeuchi K, Cheng J, Matsuki Y, Nobuta R, Gilmozzi A, Berninghausen O, Tesina P, Becker T, Coller J, Inada T, Beckmann R
Science (2020) 368 [ Pubmed: 32299921 DOI: doi:10.1126/science.aay6912 ]
- General negative regulator of transcription subunit 5 (13 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s8 (21 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s20 (11 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Saccharomyces cerevisiae s288c 18s ribosomal rna (rdn18-1), rrna (579 kDa, RNA from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l17-a (20 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s23-a (15 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l20-a (20 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l3 (43 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l37-a (9 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l9-a (21 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l24-a (14 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s17-b (14 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s1-a (26 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l11-b (19 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s10-a (11 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s4-a (29 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l22-a (11 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Ubiquitin-60s ribosomal protein l40 (6 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s28-a (7 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s16-a (15 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l1-a (22 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l29 (6 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l28 (16 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s22-a (14 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l21-a (18 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s30-a (6 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Magnesium ion (24 Da, Ligand)
- 60s ribosomal protein l32 (14 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l33-a (12 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l41-b (3 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s27-a (8 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s7-a (21 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l2-a (27 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 5.8s rrna (50 kDa, RNA from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Zinc ion (65 Da, Ligand)
- 60s ribosomal protein l13-a (21 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l38 (8 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l27-a (15 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l25 (13 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l18-a (20 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s14-b (13 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l7-a (25 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l26-a (14 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s29-a (6 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l8-a (25 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l41-a (2 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s3 (24 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s2 (23 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l30 (10 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l39 (6 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l5 (33 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Yeast 80s ribosome in complex with the not5 subunit of the ccr4-not complex (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Trna (24 kDa, RNA from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s15 (13 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 25s rrna (1 MDa, RNA from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s0-a (22 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l4-a (39 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s25-a (9 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s9-a (21 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l16-a (22 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l15-a (24 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l19-a (21 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l31-a (12 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l43-a (9 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l35-a (13 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 5s rrna (38 kDa, RNA from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l36-a (11 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Guanine nucleotide-binding protein subunit beta-like protein (34 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l42-a (11 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s11-a (16 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l23-a (14 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Mrna (935 Da, RNA from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s24-a (15 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Ubiquitin-40s ribosomal protein s31 (8 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l6-b (19 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s5 (22 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l34-a (12 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s13 (16 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s26-b (11 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s6-a (26 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l10 (25 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s21-a (9 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s19-a (15 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l14-a (14 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s12 (13 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Spermidine (145 Da, Ligand)
- 40s ribosomal protein s18-a (16 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
The architecture of the S. pombe CCR4-NOT complex
Ukleja M, Cuellar J, Siwaszek J, Kasprzak JM, Czarnocki-Cieciura M, Bujnicki J, Dziembowski A, Valpuesta JM
Nat Commun (2016) 7 pp. 10433- [ Pubmed: 26804377 DOI: doi:10.1038/ncomms10433 ]
- S. pombe ccr4-not complex (700 MDa, Sample)
- Ccr4-not complex (700 MDa, Protein from Schizosaccharomyces pombe)
Insights into the structure of the CCR4-NOT Complex by electron microscopy
Nasertorabi F, Batisse C, Diepholz M, Suck D, Bottcher B
FEBS Lett (2011) 585 pp. 2182-2186 [ Pubmed: 21669201 DOI: doi:10.1016/j.febslet.2011.05.071 ]
- Ccr4not complex (Protein from Saccharomyces cerevisiae)
- Ccr4not core-complex (800 kDa, Sample)