Cryo-EM structure of the transmembrane anti-sigma factor DdvA
Resolution: 3.11 Å
EM Method: Single-particle
Fitted PDBs: 8rot
Q-score: 0.538
Bernal-Bernal D, Pantoja-Uceda D, Lopez-Alonso JP, Lopez-Rojo A, Lopez-Ruiz JA, Galbis-Martinez M, Ochoa-Lizarralde B, Tascon I, Elias-Arnanz M, Ubarretxena-Belandia I, Padmanabhan S
Sci Adv (2024) 10 pp. eadp1053-eadp1053 [ Pubmed: 39454004 DOI: doi:10.1126/sciadv.adp1053 ]
- Chat domain-containing protein (108 kDa, Protein from Myxococcus xanthus)
- Ddva in complex with ddvs (Complex from Myxococcus xanthus)
Symmetry expansion of dimeric transmembrane anti-sigma factor DdvA
Resolution: 2.94 Å
EM Method: Single-particle
Fitted PDBs: 8rw5
Q-score: 0.577
Bernal-Bernal D, Pantoja-Uceda D, Lopez-Alonso JP, Lopez-Rojo A, Lopez-Ruiz JA, Galbis-Martinez M, Ochoa-Lizarralde B, Tascon I, Elias-Arnanz M, Ubarretxena-Belandia I, Padmanabhan S
Sci Adv (2024) 10 pp. eadp1053-eadp1053 [ Pubmed: 39454004 DOI: doi:10.1126/sciadv.adp1053 ]
- Chat domain-containing protein (108 kDa, Protein from Myxococcus xanthus)
- Ddva in complex with ddvs (Complex from Myxococcus xanthus)
Cryo-EM structure of DiCas7-11-crRNA in complex with regulator
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 8wmc
Q-score: 0.538
Hong T, Luo Q, Ma H, Wang X, Li X, Shen C, Pang J, Wang Y, Chen Y, Zhang C, Su Z, Dong H, Tang X
Signal Transduct Target Ther (2024) 9 pp. 111-111 [ DOI: doi:10.1038/s41392-024-01821-4 Pubmed: 38735995 ]
- Chat domain-containing protein (88 kDa, Protein from Desulfonema ishimotonii)
- Crispr-associated ramp family protein (183 kDa, Protein from Desulfonema ishimotonii)
- Rna (38-mer) (12 kDa, RNA from Escherichia coli)
- Zinc ion (65 Da, Ligand)
- Ternary complex of cas7-11_crrna_tpr-chat (Complex from Escherichia coli)
Cryo-EM structure of Cas7-11-crRNA bound to N-terminal of TPR-CHAT
Resolution: 2.86 Å
EM Method: Single-particle
Fitted PDBs: 8wml
Q-score: 0.522
Hong T, Luo Q, Ma H, Wang X, Li X, Shen C, Pang J, Wang Y, Chen Y, Zhang C, Su Z, Dong H, Tang X
Signal Transduct Target Ther (2024) 9 pp. 111-111 [ DOI: doi:10.1038/s41392-024-01821-4 Pubmed: 38735995 ]
- Chat domain-containing protein (7 kDa, Protein from Desulfonema ishimotonii)
- Ternary complex of cas7-11-crrna bound to n-terminal of tpr-chat (Complex from Escherichia coli)
- Crrna (38-mer) (12 kDa, RNA from Escherichia coli)
- Crispr-associated ramp family protein (183 kDa, Protein from Desulfonema ishimotonii)
- Zinc ion (65 Da, Ligand)
Structure of activated SAVED-CHAT filament
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 8tl0
Q-score: 0.485
Steens JA, Bravo JPK, Salazar CRP, Yildiz C, Amieiro AM, Kostlbacher S, Prinsen SHP, Andres AS, Patinios C, Bardis A, Barendregt A, Scheltema RA, Ettema TJG, van der Oost J, Taylor DW, Staals RHJ
Science (2024) 383 pp. 512-519 [ DOI: doi:10.1126/science.adk0378 Pubmed: 38301007 ]
- Chat domain-containing protein (54 kDa, Protein from Haliangium ochraceum)
- Rna (5'-r(*ap*ap*a)-3') (942 Da, RNA from synthetic construct)
- Active saved-chat filament (1 MDa, Complex from Haliangium ochraceum)
Cryo-EM structure of Cas7-11-crRNA-Csx29 ternary complex
Resolution: 3.29 Å
EM Method: Single-particle
Fitted PDBs: 7ynd
Q-score: 0.526
Huo Y, Zhao H, Dong Q, Jiang T
Nat Microbiol (2023) 8 pp. 522-532 [ Pubmed: 36702942 DOI: doi:10.1038/s41564-022-01316-4 ]
- Cas7-11-crrna-csx29 ternary complex (Complex from Desulfonema ishimotonii)
- Crispr-associated ramp family protein (183 kDa, Protein from Desulfonema ishimotonii)
- Crrna (38-mer) (15 kDa, RNA from Desulfonema ishimotonii)
- Chat domain-containing protein (88 kDa, Protein from Desulfonema ishimotonii)
Structure of the Cas7-11-Csx29-guide RNA complex
Resolution: 2.49 Å
EM Method: Single-particle
Fitted PDBs: 7y9x
Q-score: 0.59
Kato K, Okazaki S, Schmitt-Ulms C, Jiang K, Zhou W, Ishikawa J, Isayama Y, Adachi S, Nishizawa T, Makarova KS, Koonin EV, Abudayyeh OO, Gootenberg JS, Nishimasu H
Science (2022) 378 pp. 882-889 [ Pubmed: 36423304 DOI: doi:10.1126/science.add7347 ]
- Rna (Complex)
- Cas7-11 in complex with a crrna and its target rna (Complex from Desulfonema ishimotonii)
- Chat domain-containing protein (88 kDa, Protein from Desulfonema ishimotonii)
- Crispr-associated ramp family protein (185 kDa, Protein from Desulfonema ishimotonii)
- Crrna (12 kDa, RNA from Desulfonema ishimotonii)
- Cas7-11, csx29 (Complex from Desulfonema ishimotonii)
- Zinc ion (65 Da, Ligand)
Structure of the Cas7-11-Csx29-guide RNA-target RNA (no PFS) complex
Resolution: 2.77 Å
EM Method: Single-particle
Fitted PDBs: 7y9y
Q-score: 0.564
Kato K, Okazaki S, Schmitt-Ulms C, Jiang K, Zhou W, Ishikawa J, Isayama Y, Adachi S, Nishizawa T, Makarova KS, Koonin EV, Abudayyeh OO, Gootenberg JS, Nishimasu H
Science (2022) 378 pp. 882-889 [ DOI: doi:10.1126/science.add7347 Pubmed: 36423304 ]
- Rna (38-mer) (12 kDa, RNA from Desulfonema ishimotonii)
- Cas7-11 in complex with a crrna and its target rna (Complex from Desulfonema ishimotonii)
- Chat domain-containing protein (88 kDa, Protein from Desulfonema ishimotonii)
- Rna (27-mer) (8 kDa, RNA from Desulfonema ishimotonii)
- Crrna, target rna (Complex from Desulfonema ishimotonii)
- Zinc ion (65 Da, Ligand)
- Crispr-associated ramp family protein (185 kDa, Protein from Desulfonema ishimotonii)
- Cas7-11, csx29 (Complex)
Structure of the Cas7-11-Csx29-guide RNA-target RNA (non-matching PFS) complex
Resolution: 2.84 Å
EM Method: Single-particle
Fitted PDBs: 8gs2
Q-score: 0.571
Kato K, Okazaki S, Schmitt-Ulms C, Jiang K, Zhou W, Ishikawa J, Isayama Y, Adachi S, Nishizawa T, Makarova KS, Koonin EV, Abudayyeh OO, Gootenberg JS, Nishimasu H
Science (2022) 378 pp. 882-889 [ Pubmed: 36423304 DOI: doi:10.1126/science.add7347 ]
- Crispr-associated ramp family protein (185 kDa, Protein from Desulfonema ishimotonii)
- Chat domain-containing protein (88 kDa, Protein from Desulfonema ishimotonii)
- Crrna (30 kDa, RNA from Desulfonema ishimotonii)
- Adenosine monophosphate (347 Da, Ligand)
- Target rna with non-matching pfs (10 kDa, RNA from Desulfonema ishimotonii)
- Cas7-11 in complex with a crrna and its target rna (Complex from Desulfonema ishimotonii)
- Cytidine-5'-monophosphate (323 Da, Ligand)
- Crrna (Complex)
- Cas7-11, csx29 (Complex)
- Zinc ion (65 Da, Ligand)
- Target rna with 3'-tag (Complex from Desulfonema ishimotonii)