Cryo-EM reveals cholesterol binding in the lysosomal GPCR-like protein LYCHOS
Zhao J, Shen Q, Yong X, Li X, Tian X, Sun S, Xu Z, Zhang X, Zhang L, Yang H, Shao Z, Xu H, Jiang Y, Zhang Y, Yan W
Nat Struct Mol Biol (2025) [ Pubmed: 39824976 DOI: doi:10.1038/s41594-024-01470-9 ]
- Lysosomal cholesterol signaling protein (93 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Cholesterol-bound lychos (Complex from Homo sapiens)
- Water (18 Da, Ligand)
- [(2r)-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] octadecanoate (720 Da, Ligand)
- Sodium ion (22 Da, Ligand)
Structure of r-glutamyl carboxylase (GGCX)
Wang R, Chen B, Elghobashi-Meinhardt N, Tie J-K, Ayala A, Zhou N, Qi X
Nature (2025) [ DOI: doi:10.1038/s41586-024-08484-9 ]
- Cholesterol (386 Da, Ligand)
- Ggcx protein (Complex from Homo sapiens)
- Vitamin k-dependent gamma-carboxylase (88 kDa, Protein from Homo sapiens)
Wang R, Chen B, Elghobashi-Meinhardt N, Tie J-K, Ayala A, Zhou N, Qi X
Nature (2025) [ DOI: doi:10.1038/s41586-024-08484-9 ]
- Ggcx-bgp complex (Complex from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Vitamin k-dependent gamma-carboxylase (88 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- (2s)-3-(hexadecanoyloxy)-2-[(9z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate (760 Da, Ligand)
- Osteocalcin (11 kDa, Protein from Homo sapiens)
Structure of GGCX-BGP-menaquinone-4 epoxide complex
Wang R, Chen B, Elghobashi-Meinhardt N, Tie J-K, Ayala A, Zhou N, Qi X
Nature (2025) [ DOI: doi:10.1038/s41586-024-08484-9 ]
- Cholesterol (386 Da, Ligand)
- Vitamin k-dependent gamma-carboxylase (88 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- (1ar,7as)-1a-methyl-7a-[(2e,6e,10e)-3,7,11,15-tetramethylhexadeca-2,6,10,14-tetraen-1-yl]-1a,7a-dihydronaphtho[2,3-b]oxirene-2,7-dione (460 Da, Ligand)
- Ggcx-bgp-menaquinone-4 epoxide complex (Complex from Homo sapiens)
- Osteocalcin (11 kDa, Protein from Homo sapiens)
- (2s)-3-(hexadecanoyloxy)-2-[(9z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate (760 Da, Ligand)
Cryo-EM structure of human dimeric Apelin receptor.
Resolution: 3.25 Å
EM Method: Single-particle
Fitted PDBs: 8xqi
Q-score: 0.454
Yue Y, Liu L, Wu L, Xu C, Na M, Liu S, Liu Y, Li F, Liu J, Shi S, Lei H, Zhao M, Yang T, Ji W, Wang A, Hanson MA, Stevens RC, Liu J, Xu F
Nat Commun (2025) 16 pp. 310-310 [ Pubmed: 39747115 DOI: doi:10.1038/s41467-024-55555-6 ]
- Homodimer of apelin receptor (Complex from Homo sapiens)
- Soluble cytochrome b562,apelin receptor (54 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
Cryo-EM structure of human dimeric APJR complex with antagonistic antibody
Yue Y, Liu L, Wu L, Xu C, Na M, Liu S, Liu Y, Li F, Liu J, Shi S, Lei H, Zhao M, Yang T, Ji W, Wang A, Hanson MA, Stevens RC, Liu J, Xu F
Nat Commun (2025) 16 pp. 310-310 [ DOI: doi:10.1038/s41467-024-55555-6 Pubmed: 39747115 ]
- Apjr complex with antibody (Complex from Homo sapiens)
- Jn241 (17 kDa, Protein from Homo sapiens)
- Soluble cytochrome b562,apelin receptor (54 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
Cryo-EM structure of APJR complex with agonistic antibody
Yue Y, Liu L, Wu L, Xu C, Na M, Liu S, Liu Y, Li F, Liu J, Shi S, Lei H, Zhao M, Yang T, Ji W, Wang A, Hanson MA, Stevens RC, Liu J, Xu F
Nat Commun (2025) 16 pp. 310-310 [ DOI: doi:10.1038/s41467-024-55555-6 Pubmed: 39747115 ]
- Apjr complex with antibody (Complex from Homo sapiens)
- Agonistic antibody (13 kDa, Protein from Homo sapiens)
- Soluble cytochrome b562,apelin receptor (54 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
Cryo-EM structure of the mouse TRPM3 alpha 2 channel in complex with primidone
Yin Y, Park CG, Feng S, Guan Z, Lee HJ, Zhang F, Sharma K, Borgnia MJ, Im W, Lee SY
Nat Struct Mol Biol (2025) [ DOI: doi:10.1038/s41594-024-01463-8 Pubmed: 39809942 ]
- Transient receptor potential cation channel, subfamily m, member 3 (198 kDa, Protein from Mus musculus)
- Cholesterol hemisuccinate (486 Da, Ligand)
- [(2~{r})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{z})-octadec-9-enoate (717 Da, Ligand)
- Transient receptor potential cation channel subfamily m member 3 (Complex from Mus musculus)
- Primidone (218 Da, Ligand)
Cryo-EM structure of the mouse TRPM3 alpha 2 channel in complex with cholesteryl hemisuccinate
Yin Y, Park CG, Feng S, Guan Z, Lee HJ, Zhang F, Sharma K, Borgnia MJ, Im W, Lee SY
Nat Struct Mol Biol (2025) [ DOI: doi:10.1038/s41594-024-01463-8 Pubmed: 39809942 ]
- 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (760 Da, Ligand)
- Transient receptor potential cation channel, subfamily m, member 3 (198 kDa, Protein from Mus musculus)
- Cholesterol hemisuccinate (486 Da, Ligand)
- [(2~{r})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{z})-octadec-9-enoate (717 Da, Ligand)
- 1,2-dilauroyl-sn-glycero-3-phosphate (535 Da, Ligand)
- Transient receptor potential cation channel subfamily m member 3 (Complex from Mus musculus)
Human proton sensing receptor GPR68 in complex with miniGs
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 9bhm
Q-score: 0.456
Howard MK, Hoppe N, Huang XP, Mitrovic D, Billesbolle CB, Macdonald CB, Mehrotra E, Rockefeller Grimes P, Trinidad DD, Delemotte L, English JG, Coyote-Maestas W, Manglik A
Cell (2024) [ Pubmed: 39753132 DOI: doi:10.1016/j.cell.2024.11.036 ]
- Cholesterol hemisuccinate (486 Da, Ligand)
- Ovarian cancer g-protein coupled receptor 1 (42 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (30 kDa, Protein from Homo sapiens)
- Complex of gpr68 bound to gs heterotrimer (Complex)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Homo sapiens)
- Nanobody 35 (15 kDa, Protein from Lama glama)