mouse TMEM63b in LMNG-CHS micelle
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 8wg3
Q-score: 0.456
Miyata Y, Takahashi K, Lee Y, Sultan CS, Kuribayashi R, Takahashi M, Hata K, Bamba T, Izumi Y, Liu K, Uemura T, Nomura N, Iwata S, Nagata S, Nishizawa T, Segawa K
To Be Published
- Mouse tmem63b (100 kDa, Complex from Mus musculus)
- 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (760 Da, Ligand)
- Csc1-like protein 2,green fluorescent protein (126 kDa, Protein from Aequorea victoria)
- Cholesterol hemisuccinate (486 Da, Ligand)
mouse TMEM63b in DDM-CHS micelle with YN9303-24 Fab
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 8wg4
Q-score: 0.478
Miyata Y, Takahashi K, Lee Y, Sultan CS, Kuribayashi R, Takahashi M, Hata K, Bamba T, Izumi Y, Liu K, Uemura T, Nomura N, Iwata S, Nagata S, Nishizawa T, Segawa K
To Be Published
- Mouse tmem63b (100 kDa, Complex from Mus musculus)
- 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (760 Da, Ligand)
- Csc1-like protein 2,green fluorescent protein (126 kDa, Protein from Aequorea victoria)
- Cholesterol hemisuccinate (486 Da, Ligand)
Cryo-EM structure of non gastric H,K-ATPase alpha2 K794A in (K+)E2-AlF state
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 7x21
Q-score: 0.566
Young VC, Nakanishi H, Meyer DJ, Nishizawa T, Oshima A, Artigas P, Abe K
Nat Commun (2022) 13 pp. 5270-5270 [ DOI: doi:10.1038/s41467-022-32793-0 Pubmed: 36085139 ]
- Water (18 Da, Ligand)
- Sodium/potassium-transporting atpase subunit beta-1 (37 kDa, Protein from Rattus norvegicus)
- Tetrafluoroaluminate ion (102 Da, Ligand)
- Potassium ion (39 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Cholesterol (386 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Potassium-transporting atpase alpha chain 2 (109 kDa, Protein from Rattus norvegicus)
- Non gastric h,k-atpase (150 MDa, Complex from Rattus norvegicus)
- 1,2-dioleoyl-sn-glycero-3-phosphocholine (787 Da, Ligand)
Cryo-EM structure of non gastric H,K-ATPase alpha2 K794S in (2K+)E2-AlF state
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7x22
Q-score: 0.508
Young VC, Nakanishi H, Meyer DJ, Nishizawa T, Oshima A, Artigas P, Abe K
Nat Commun (2022) 13 pp. 5270-5270 [ DOI: doi:10.1038/s41467-022-32793-0 Pubmed: 36085139 ]
- Water (18 Da, Ligand)
- Sodium/potassium-transporting atpase subunit beta-1 (37 kDa, Protein from Rattus norvegicus)
- Tetrafluoroaluminate ion (102 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Potassium ion (39 Da, Ligand)
- Cholesterol (386 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Potassium-transporting atpase alpha chain 2 (109 kDa, Protein from Rattus norvegicus)
- Non gastric h,k-atpase (150 MDa, Complex from Rattus norvegicus)
- 1,2-dioleoyl-sn-glycero-3-phosphocholine (787 Da, Ligand)
Cryo-EM structure of non gastric H,K-ATPase alpha2 SPWC mutant in (2K+)E2-AlF state
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 7x24
Q-score: 0.515
Young VC, Nakanishi H, Meyer DJ, Nishizawa T, Oshima A, Artigas P, Abe K
Nat Commun (2022) 13 pp. 5270-5270 [ DOI: doi:10.1038/s41467-022-32793-0 Pubmed: 36085139 ]
- Sodium/potassium-transporting atpase subunit beta-1 (37 kDa, Protein from Rattus norvegicus)
- Tetrafluoroaluminate ion (102 Da, Ligand)
- Potassium ion (39 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Cholesterol (386 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Non gastric h,k-atpase (150 MDa, Complex from Rattus norvegicus)
- 1,2-dioleoyl-sn-glycero-3-phosphocholine (787 Da, Ligand)
- Potassium-transporting atpase alpha chain 2 (109 kDa, Protein from Rattus norvegicus)
Thermostabilized human prestin in complex with chloride
Resolution: 3.52 Å
EM Method: Single-particle
Fitted PDBs: 7v73
Q-score: 0.488
Futamata H, Fukuda M, Umeda R, Yamashita K, Tomita A, Takahashi S, Shikakura T, Hayashi S, Kusakizako T, Nishizawa T, Homma K, Nureki O
Nat Commun (2022) 13 pp. 6208-6208 [ Pubmed: 36266333 DOI: doi:10.1038/s41467-022-34017-x ]
- Chloride ion (35 Da, Ligand)
- 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (760 Da, Ligand)
- Cholesterol (386 Da, Ligand)
- Prestin in complex with chloride ion (Complex from Homo sapiens)
- Prestin (82 kDa, Protein from Homo sapiens)
Thermostabilized human prestin in complex with sulfate
Resolution: 3.63 Å
EM Method: Single-particle
Fitted PDBs: 7v74
Q-score: 0.5
Futamata H, Fukuda M, Umeda R, Yamashita K, Tomita A, Takahashi S, Shikakura T, Hayashi S, Kusakizako T, Nishizawa T, Homma K, Nureki O
Nat Commun (2022) 13 pp. 6208-6208 [ Pubmed: 36266333 DOI: doi:10.1038/s41467-022-34017-x ]
- 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (760 Da, Ligand)
- Sulfate ion (96 Da, Ligand)
- Cholesterol (386 Da, Ligand)
- Prestin (82 kDa, Protein from Homo sapiens)
- Prestin in complex with chloride ion (Complex from Homo sapiens)
Thermostabilized human prestin in complex with salicylate
Resolution: 3.57 Å
EM Method: Single-particle
Fitted PDBs: 7v75
Q-score: 0.488
Futamata H, Fukuda M, Umeda R, Yamashita K, Tomita A, Takahashi S, Shikakura T, Hayashi S, Kusakizako T, Nishizawa T, Homma K, Nureki O
Nat Commun (2022) 13 pp. 6208-6208 [ Pubmed: 36266333 DOI: doi:10.1038/s41467-022-34017-x ]
- 2-hydroxybenzoic acid (138 Da, Ligand)
- 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (760 Da, Ligand)
- Cholesterol (386 Da, Ligand)
- Prestin (82 kDa, Protein from Homo sapiens)
- Prestin in complex with chloride ion (Complex from Homo sapiens)
Inward-facing structure of human EAAT2 in the WAY213613-bound state
Resolution: 3.49 Å
EM Method: Single-particle
Fitted PDBs: 7vr7
Q-score: 0.553
Kato T, Kusakizako T, Jin C, Zhou X, Ohgaki R, Quan L, Xu M, Okuda S, Kobayashi K, Yamashita K, Nishizawa T, Kanai Y, Nureki O
Nat Commun (2022) 13 pp. 4714-4714 [ Pubmed: 35953475 DOI: doi:10.1038/s41467-022-32442-6 ]
- (3beta,14beta,17beta,25r)-3-[4-methoxy-3-(methoxymethyl)butoxy]spirost-5-en (544 Da, Ligand)
- (2s)-2-azanyl-4-[[4-[2-bromanyl-4,5-bis(fluoranyl)phenoxy]phenyl]amino]-4-oxidanylidene-butanoic acid (415 Da, Ligand)
- Eaat2 (Complex from Homo sapiens)
- 1,2-diacyl-sn-glycero-3-phosphocholine (790 Da, Ligand)
- Cholesterol (386 Da, Ligand)
- Excitatory amino acid transporter 2 (63 kDa, Protein from Homo sapiens)
Inward-facing structure of human EAAT2 in the substrate-free state
Resolution: 3.58 Å
EM Method: Single-particle
Fitted PDBs: 7vr8
Q-score: 0.431
Kato T, Kusakizako T, Jin C, Zhou X, Ohgaki R, Quan L, Xu M, Okuda S, Kobayashi K, Yamashita K, Nishizawa T, Kanai Y, Nureki O
Nat Commun (2022) 13 pp. 4714-4714 [ Pubmed: 35953475 DOI: doi:10.1038/s41467-022-32442-6 ]
- (3beta,14beta,17beta,25r)-3-[4-methoxy-3-(methoxymethyl)butoxy]spirost-5-en (544 Da, Ligand)
- Eaat2 (Complex from Homo sapiens)
- 1,2-diacyl-sn-glycero-3-phosphocholine (790 Da, Ligand)
- Cholesterol (386 Da, Ligand)
- Excitatory amino acid transporter 2 (63 kDa, Protein from Homo sapiens)