Cryo-EM structure of gamma secretase in complex with a Notch fragment
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 6idf
Q-score: 0.545
Yang G, Zhou R, Zhou Q, Guo X, Yan C, Ke M, Lei J, Shi Y
Nature (2019) 565 pp. 192-197 [ DOI: doi:10.1038/s41586-018-0813-8 Pubmed: 30598546 ]
- Notch1 (14 kDa, Protein from Homo sapiens)
- Nicastrin (78 kDa, Protein from Homo sapiens)
- Gamma-secretase subunit pen-2 (12 kDa, Protein from Homo sapiens)
- N-acetyl-d-glucosamine (221 Da, Ligand)
- 1,2-diacyl-sn-glycero-3-phosphocholine (790 Da, Ligand)
- Cholesterol (386 Da, Ligand)
- Gamma-secretase subunit aph-1a (29 kDa, Protein from Homo sapiens)
- Beta-d-mannose (180 Da, Ligand)
- Gamma-secretase (Complex from Homo sapiens)
- Presenilin-1 (52 kDa, Protein from Homo sapiens)
3.7 angstrom cryoEM structure of full length human TRPM4
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 6bwi
Q-score: 0.414
Duan J, Li Z, Li J, Santa-Cruz A, Sanchez-Martinez S, Zhang J, Clapham DE
PNAS (2018) 115 pp. 2377-2382 [ Pubmed: 29463718 DOI: doi:10.1073/pnas.1722038115 ]
- Sodium ion (22 Da, Ligand)
- Transient receptor potential cation channel subfamily m member 4 (104 kDa, Protein from Homo sapiens)
- Cholesterol hemisuccinate (486 Da, Ligand)
- Human full length trpm4 (Complex from Mus musculus)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
Structural basis for cholesterol transport-like activity of the Hedgehog receptor Patched
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 6mg8
Q-score: 0.458
Zhang Y, Bulkley DP, Xin Y, Roberts KJ, Asarnow DE, Sharma A, Myers BR, Cho W, Cheng Y, Beachy PA
Cell (2018) 175 pp. 1352-1364.e14 [ DOI: doi:10.1016/j.cell.2018.10.026 Pubmed: 30415841 ]
- Cholesterol (386 Da, Ligand)
- Patched1 protein solubilized in amphipol (145 kDa, Complex from Homo sapiens)
- Protein patched homolog 1 (145 kDa, Protein from Mus musculus)
Complex of human cystic fibrosis transmembrane conductance regulator (CFTR) and GLPG1837
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 6o1v
Q-score: 0.482
Liu F, Zhang Z, Levit A, Levring J, Touhara KK, Shoichet BK, Chen J
Science (2019) 364 pp. 1184-1188 [ Pubmed: 31221859 DOI: doi:10.1126/science.aaw7611 ]
- Cystic fibrosis transmembrane conductance regulator (169 kDa, Protein from Homo sapiens)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Cholesterol (386 Da, Ligand)
- Unknown peptide (1 kDa, Protein from Homo sapiens)
- N-(3-carbamoyl-5,5,7,7-tetramethyl-4,7-dihydro-5h-thieno[2,3-c]pyran-2-yl)-1h-pyrazole-3-carboxamide (348 Da, Ligand)
- Complex of human cystic fibrosis transmembrane conductance regulator (cftr) and glpg1837 (168 kDa, Complex from Homo sapiens)
- (2s)-3-(hexadecanoyloxy)-2-[(9z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate (760 Da, Ligand)
Phosphorylated, ATP-bound human cystic fibrosis transmembrane conductance regulator (CFTR)
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 6msm
Q-score: 0.476
PNAS (2018) 115 pp. 12757-12762 [ DOI: doi:10.1073/pnas.1815287115 Pubmed: 30459277 ]
- Cystic fibrosis transmembrane conductance regulator (169 kDa, Protein from Homo sapiens)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Cholesterol (386 Da, Ligand)
- Piece of molecule-1 (1 kDa, Protein from Homo sapiens)
- Human cystic fibrosis transmembrane conductance regulator (cftr) (168 kDa, Complex from Homo sapiens)
- (2s)-3-(hexadecanoyloxy)-2-[(9z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate (760 Da, Ligand)
3.3 angstrom structure of mouse TRPM7 with EDTA
Resolution: 3.28 Å
EM Method: Single-particle
Fitted PDBs: 5zx5
Q-score: 0.511
Duan J, Li Z, Li J, Hulse RE, Santa-Cruz A, Valinsky WC, Abiria SA, Krapivinsky G, Zhang J, Clapham DE
PNAS (2018) 115 pp. E8201-E8210 [ DOI: doi:10.1073/pnas.1810719115 Pubmed: 30108148 ]
3.7 angstrom cryoEM structure of truncated mouse TRPM7
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 6bwd
Q-score: 0.418
Duan J, Li Z, Li J, Hulse RE, Santa-Cruz A, Valinsky WC, Abiria SA, Krapivinsky G, Zhang J, Clapham DE
PNAS (2018) 115 pp. E8201-E8210 [ DOI: doi:10.1073/pnas.1810719115 Pubmed: 30108148 ]
- Water (18 Da, Ligand)
- Cholesterol hemisuccinate (486 Da, Ligand)
- Transient receptor potential cation channel subfamily m member 7 (107 kDa, Protein from Mus musculus)
- Magnesium ion (24 Da, Ligand)
- Truncated mouse trpm7 with mg2+ (Complex from Mus musculus)
Cryo-EM structure of human Ptch1
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 6dmb
Q-score: 0.408
Gong X, Qian H, Cao P, Zhao X, Zhou Q, Lei J, Yan N
Science (2018) 361 [ Pubmed: 29954986 DOI: doi:10.1126/science.aas8935 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Patch1 (Cellular component from Homo sapiens)
- Protein patched homolog 1 (150 kDa, Protein from Homo sapiens)
- Cholesterol hemisuccinate (486 Da, Ligand)
Cryo-EM structure of human Ptch1 and ShhN complex
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 6dmy
Q-score: 0.388
Gong X, Qian H, Cao P, Zhao X, Zhou Q, Lei J, Yan N
Science (2018) 361 [ Pubmed: 29954986 DOI: doi:10.1126/science.aas8935 ]
- Protein patched homolog 1 (150 kDa, Protein from Homo sapiens)
- Cholesterol hemisuccinate (486 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- Patch1 (Cellular component from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Serine (105 Da, Ligand)
- Sonic hedgehog protein (19 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
Resolution: 3.86 Å
EM Method: Single-particle
Fitted PDBs: 6d6t
Q-score: 0.46
Zhu S, Noviello CM, Teng J, Walsh RM, Kim JJ, Hibbs RE
Nature (2018) 559 pp. 67-72 [ DOI: doi:10.1038/s41586-018-0255-3 Pubmed: 29950725 ]
- Gamma-amino-butanoic acid (103 Da, Ligand)
- Human gaba-a receptor subunit gamma-2 (40 kDa, Protein from Homo sapiens)
- Gamma-aminobutyric acid receptor subunit alpha-1,gamma-aminobutyric acid receptor subunit alpha-1 (41 kDa, Protein from Homo sapiens)
- Cholesterol hemisuccinate (486 Da, Ligand)
- Igg2b fab heavy chain (49 kDa, Protein from Mus musculus)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Human gaba-a receptor alpha1-beta2-gamma2 subtype in complex with gaba and flumazenil, conformation b (348 kDa, Complex from Homo sapiens)
- Gamma-aminobutyric acid receptor subunit beta-2,gamma-aminobutyric acid receptor subunit beta-2 (39 kDa, Protein from Homo sapiens)
- Kappa fab light chain (23 kDa, Protein from Mus musculus)
- Ethyl 8-fluoro-5-methyl-6-oxo-5,6-dihydro-4h-imidazo[1,5-a][1,4]benzodiazepine-3-carboxylate (303 Da, Ligand)