Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7sx3
Q-score: 0.47
Kschonsak M, Chua HC, Weidling C, Chakouri N, Noland CL, Schott K, Chang T, Tam C, Patel N, Arthur CP, Leitner A, Ben-Johny M, Ciferri C, Pless SA, Payandeh J
Nature (2022) 603 pp. 180-186 [ Pubmed: 34929720 DOI: doi:10.1038/s41586-021-04313-5 ]
- (1r)-2-{[{[(2s)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(palmitoyloxy)methyl]ethyl (11e)-octadec-11-enoate (749 Da, Ligand)
- Pentameric complex of the leak channel nalcn with fam155a, calmodulin, unc79 and unc80 (Complex from Homo sapiens)
- Calmodulin-1 (16 kDa, Protein from Homo sapiens)
- Cholesterol hemisuccinate (486 Da, Ligand)
- (1s)-2-{[(2-aminoethoxy)(hydroxy)phosphoryl]oxy}-1-[(palmitoyloxy)methyl]ethyl stearate (720 Da, Ligand)
- Transmembrane protein fam155a (54 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Sodium leak channel non-selective protein,enhanced green fluorescent protein (234 kDa, Protein from Homo sapiens)
- Unc79,protein unc-79 homolog,protein unc-79 homolog (285 kDa, Protein from Homo sapiens)
- Protein unc-80 homolog (366 kDa, Protein from Homo sapiens)
Human NALCN-FAM155A-UNC79-UNC80 channelosome with CaM bound, conformation 2/2
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 7sx4
Q-score: 0.359
Kschonsak M, Chua HC, Weidling C, Chakouri N, Noland CL, Schott K, Chang T, Tam C, Patel N, Arthur CP, Leitner A, Ben-Johny M, Ciferri C, Pless SA, Payandeh J
Nature (2022) 603 pp. 180-186 [ Pubmed: 34929720 DOI: doi:10.1038/s41586-021-04313-5 ]
- (1r)-2-{[{[(2s)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(palmitoyloxy)methyl]ethyl (11e)-octadec-11-enoate (749 Da, Ligand)
- Pentameric complex of the leak channel nalcn with fam155a, calmodulin, unc79 and unc80 (Complex from Homo sapiens)
- Calmodulin-1 (16 kDa, Protein from Homo sapiens)
- Cholesterol hemisuccinate (486 Da, Ligand)
- (1s)-2-{[(2-aminoethoxy)(hydroxy)phosphoryl]oxy}-1-[(palmitoyloxy)methyl]ethyl stearate (720 Da, Ligand)
- Transmembrane protein fam155a (54 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Sodium leak channel non-selective protein,enhanced green fluorescent protein (234 kDa, Protein from Homo sapiens)
- Unc79,protein unc-79 homolog (285 kDa, Protein from Homo sapiens)
- Protein unc-80 homolog (366 kDa, Protein from Homo sapiens)
Human Neurokinin 1 receptor (NK1R) substance P Gq chimera (mGsqi) complex
Resolution: 2.71 Å
EM Method: Single-particle
Fitted PDBs: 7p00
Q-score: 0.546
Thom C, Ehrenmann J, Vacca S, Waltenspuhl Y, Schoppe J, Medalia O, Pluckthun A
Sci Adv (2021) 7 pp. eabk2872-eabk2872 [ DOI: doi:10.1126/sciadv.abk2872 Pubmed: 34878828 ]
- Antibody fragment scfv16 (32 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Substance-p receptor (44 kDa, Protein from Homo sapiens)
- Substance p (1 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Guanine nucleotide-binding protein g(i) subunit alpha-1,guanine nucleotide-binding protein g(s) subunit alpha isoforms short (28 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Nk1r in complex with substance p, heterotrimeric mini-gq chimera (gsqi) and scfv16 (Complex from Homo sapiens)
Human Neurokinin 1 receptor (NK1R) substance P Gs complex
Resolution: 2.87 Å
EM Method: Single-particle
Fitted PDBs: 7p02
Q-score: 0.502
Thom C, Ehrenmann J, Vacca S, Waltenspuhl Y, Schoppe J, Medalia O, Pluckthun A
Sci Adv (2021) 7 pp. eabk2872-eabk2872 [ DOI: doi:10.1126/sciadv.abk2872 Pubmed: 34878828 ]
- Antibody fragment scfv16 (32 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Substance-p receptor (44 kDa, Protein from Homo sapiens)
- Protachykinin-1 (1 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Nk1r in complex with substance p, heterotrimeric mini-gs chimera (gsi) and scfv16 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1,guanine nucleotide-binding protein g(s) subunit alpha isoforms short (28 kDa, Protein from Homo sapiens)
Ca2+ bound Drosophila Slo channel
Resolution: 2.38 Å
EM Method: Single-particle
Fitted PDBs: 7pxe
Q-score: 0.604
Raisch T, Brockmann A, Ebbinghaus-Kintscher U, Freigang J, Gutbrod O, Kubicek J, Maertens B, Hofnagel O, Raunser S
Nat Commun (2021) 12 pp. 7164-7164 [ Pubmed: 34887422 DOI: doi:10.1038/s41467-021-27435-w ]
- Slo tetramer (Complex from Drosophila melanogaster)
- Water (18 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Potassium ion (39 Da, Ligand)
- Cholesterol (386 Da, Ligand)
- Isoform j of calcium-activated potassium channel slowpoke (130 kDa, Protein from Drosophila melanogaster)
- (4s,7r)-4-hydroxy-n,n,n-trimethyl-9-oxo-7-[(palmitoyloxy)methyl]-3,5,8-trioxa-4-phosphahexacosan-1-aminium 4-oxide (763 Da, Ligand)
Verruculogen-bound Drosophila Slo channel
Resolution: 2.73 Å
EM Method: Single-particle
Fitted PDBs: 7pxg
Q-score: 0.552
Raisch T, Brockmann A, Ebbinghaus-Kintscher U, Freigang J, Gutbrod O, Kubicek J, Maertens B, Hofnagel O, Raunser S
Nat Commun (2021) 12 pp. 7164-7164 [ Pubmed: 34887422 DOI: doi:10.1038/s41467-021-27435-w ]
- Slo tetramer (Complex from Drosophila melanogaster)
- Calcium ion (40 Da, Ligand)
- Verruculogen (511 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Potassium ion (39 Da, Ligand)
- Cholesterol (386 Da, Ligand)
- Isoform j of calcium-activated potassium channel slowpoke (130 kDa, Protein from Drosophila melanogaster)
- (4s,7r)-4-hydroxy-n,n,n-trimethyl-9-oxo-7-[(palmitoyloxy)methyl]-3,5,8-trioxa-4-phosphahexacosan-1-aminium 4-oxide (763 Da, Ligand)
Emodepside-bound Drosophila Slo channel
Resolution: 2.59 Å
EM Method: Single-particle
Fitted PDBs: 7pxh
Q-score: 0.569
Raisch T, Brockmann A, Ebbinghaus-Kintscher U, Freigang J, Gutbrod O, Kubicek J, Maertens B, Hofnagel O, Raunser S
Nat Commun (2021) 12 pp. 7164-7164 [ Pubmed: 34887422 DOI: doi:10.1038/s41467-021-27435-w ]
- Slo tetramer (Complex from Drosophila melanogaster)
- (3~{s},6~{r},9~{s},12~{r},15~{s},18~{r},21~{s},24~{r})-4,6,10,16,18,22-hexamethyl-3,9,15,21-tetrakis(2-methylpropyl)-12,24-bis[(4-morpholin-4-ylphenyl)methyl]-1,7,13,19-tetraoxa-4,10,16,22-tetrazacyclotetracosane-2,5,8,11,14,17,20,23-octone (1 kDa, Ligand)
- Calcium ion (40 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Potassium ion (39 Da, Ligand)
- Cholesterol (386 Da, Ligand)
- Isoform j of calcium-activated potassium channel slowpoke (130 kDa, Protein from Drosophila melanogaster)
- (4s,7r)-4-hydroxy-n,n,n-trimethyl-9-oxo-7-[(palmitoyloxy)methyl]-3,5,8-trioxa-4-phosphahexacosan-1-aminium 4-oxide (763 Da, Ligand)
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 7li7
Q-score: 0.364
Sci Adv (2021) 7 pp. eabl3857-eabl3857 [ DOI: doi:10.1126/sciadv.abl3857 Pubmed: 34851672 ]
- Dodecane (170 Da, Ligand)
- Pentane (72 Da, Ligand)
- Heptane (100 Da, Ligand)
- Variable domain of 15b8 antibody fab light chain (11 kDa, Protein from Mus musculus)
- Cholesterol (386 Da, Ligand)
- Variable domain of 15b8 antibody fab heavy chain (12 kDa, Protein from Mus musculus)
- Sodium-dependent serotonin transporter (60 kDa, Protein from Homo sapiens)
- Decane (142 Da, Ligand)
- Apo human serotonin transporter in complex with 15b8 fab in nacl (Complex from Homo sapiens)
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7lia
Q-score: 0.498
Sci Adv (2021) 7 pp. eabl3857-eabl3857 [ DOI: doi:10.1126/sciadv.abl3857 Pubmed: 34851672 ]
- Chloride ion (35 Da, Ligand)
- Sodium ion (22 Da, Ligand)
- Heptane (100 Da, Ligand)
- Variable domain of 15b8 antibody fab light chain (11 kDa, Protein from Mus musculus)
- Sodium-dependent serotonin transporter (60 kDa, Protein from Homo sapiens)
- Pentane (72 Da, Ligand)
- Human serotonin transporter in complex with 15b8 fab in presence of serotonin in nacl (Complex from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Variable domain of 15b8 antibody fab heavy chain (12 kDa, Protein from Mus musculus)
- Serotonin (176 Da, Ligand)
- Decane (142 Da, Ligand)
5-HT bound serotonin transporter reconstituted in lipid nanodisc in NaCl in occluded conformation
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 7mgw
Q-score: 0.362
Sci Adv (2021) 7 pp. eabl3857-eabl3857 [ DOI: doi:10.1126/sciadv.abl3857 Pubmed: 34851672 ]
- Chloride ion (35 Da, Ligand)
- Dodecane (170 Da, Ligand)
- Pentane (72 Da, Ligand)
- Heptane (100 Da, Ligand)
- Cholesterol (386 Da, Ligand)
- Variable domain of 15b8 antiboty fab light chain (11 kDa, Protein from Mus musculus)
- Variable domain of 15b8 antibody fab heavy chain (12 kDa, Protein from Mus musculus)
- Sodium-dependent serotonin transporter (60 kDa, Protein from Homo sapiens)
- Serotonin (176 Da, Ligand)
- Decane (142 Da, Ligand)
- Apo human serotonin transporter in complex with 15b8 fab in nacl (Complex from Homo sapiens)