Cryo-EM structure of GeoCas9-sgRNA-dsDNA ternary complex
Resolution: 3.08 Å
EM Method: Single-particle
Fitted PDBs: 8jtj
Q-score: 0.421
Shen P, Zhang L, Liu B, Li X, Wang C, Min J, Huang JW, Chen CC, Guo RT
ACS Catal (2024) 14 pp. 13227-13235 [ DOI: doi:10.1021/acscatal.4c03278 ]
- Rna (139-mer) (44 kDa, RNA from Geobacillus stearothermophilus)
- Dna (5'-d(p*gp*gp*gp*cp*gp*cp*gp*ap*a)-3') (2 kDa, DNA from Geobacillus stearothermophilus)
- Type ii crispr rna-guided endonuclease cas9 (130 kDa, Complex from Geobacillus stearothermophilus)
- Crispr-associated endonuclease cas9 (128 kDa, Protein from Geobacillus stearothermophilus)
- Dna (29-mer) (8 kDa, DNA from Geobacillus stearothermophilus)
Cryo-EM structure of GeoCas9-sgRNA binary complex
Resolution: 3.21 Å
EM Method: Single-particle
Fitted PDBs: 8jtr
Q-score: 0.401
Shen P, Zhang L, Liu B, Li X, Wang C, Min J, Huang JW, Chen CC, Guo RT
ACS Catal (2024) 14 pp. 13227-13235 [ DOI: doi:10.1021/acscatal.4c03278 ]
- Crispr-associated endonuclease cas9 (128 kDa, Protein from Geobacillus stearothermophilus)
- Sgrna (139-bp) (44 kDa, RNA from Geobacillus stearothermophilus)
- Type ii crispr rna-guided endonuclease cas9 (130 kDa, Complex from Geobacillus stearothermophilus)
CRISPR-Cas type III-D effector complex
Resolution: 2.52 Å
EM Method: Single-particle
Fitted PDBs: 8s9t
Q-score: 0.623
Schwartz EA, Bravo JPK, Ahsan M, Macias LA, McCafferty CL, Dangerfield TL, Walker JN, Brodbelt JS, Palermo G, Fineran PC, Fagerlund RD, Taylor DW
Nat Commun (2024) 15 pp. 3324-3324 [ DOI: doi:10.1038/s41467-024-47506-y Pubmed: 38637512 ]
- Crispr rna (11 kDa, RNA from Synechocystis sp. PCC 6803)
- Cas7-2x (56 kDa, Protein from Synechocystis sp. PCC 6803)
- Crispr-cas type iii-d effector complex (332 kDa, Complex from Synechocystis sp. PCC 6803)
- Tigr03984 family crispr-associated protein (21 kDa, Protein from Synechocystis sp. PCC 6803)
- Cas10 (64 kDa, Protein from Synechocystis sp. PCC 6803)
- Cas7-cas5-cas11 (87 kDa, Protein from Synechocystis sp. PCC 6803)
- Tigr03986 family crispr-associated ramp protein (90 kDa, Protein from Synechocystis sp. PCC 6803)
CRISPR-Cas type III-D effector complex bound to a target RNA
Resolution: 2.77 Å
EM Method: Single-particle
Fitted PDBs: 8s9u
Q-score: 0.584
Schwartz EA, Bravo JPK, Ahsan F, Macias LA, Brodbelt JS, Brodbelt JS, Palermo G, Fineran PC, Fagerlund RD, Taylor DW
To Be Published
- Crispr rna (11 kDa, RNA from Synechocystis sp. PCC 6803)
- Crispr-cas type iii-d effector complex bound to target rna (351 kDa, Complex from Synechocystis sp. PCC 6803)
- Cas7-2x (56 kDa, Protein from Synechocystis sp. PCC 6803)
- Tigr03984 family crispr-associated protein (21 kDa, Protein from Synechocystis sp. PCC 6803)
- Cas10 (64 kDa, Protein from Synechocystis sp. PCC 6803)
- Cas7-cas5-cas11 (87 kDa, Protein from Synechocystis sp. PCC 6803)
- Target rna (19 kDa, RNA from Synechocystis sp. PCC 6803)
- Tigr03986 family crispr-associated ramp protein (90 kDa, Protein from Synechocystis sp. PCC 6803)
CRISPR-Cas type III-D effector complex bound to a self-target RNA in the pre-cleavage state
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 8s9v
Q-score: 0.561
Schwartz EA, Bravo JPK, Ahsan M, Macias LA, McCafferty CL, Dangerfield TL, Walker JN, Brodbelt JS, Palermo G, Fineran PC, Fagerlund RD, Taylor DW
Nat Commun (2024) 15 pp. 3324-3324 [ DOI: doi:10.1038/s41467-024-47506-y Pubmed: 38637512 ]
- Crispr rna (11 kDa, RNA from Synechocystis sp. PCC 6803)
- Cas7-2x (56 kDa, Protein from Synechocystis sp. PCC 6803)
- Crispr-cas type iii-d effector complex (332 kDa, Complex from Synechocystis sp. PCC 6803)
- Tigr03984 family crispr-associated protein (21 kDa, Protein from Synechocystis sp. PCC 6803)
- Cas10 (64 kDa, Protein from Synechocystis sp. PCC 6803)
- Magnesium ion (24 Da, Ligand)
- Cas7-cas5-cas11 (87 kDa, Protein from Synechocystis sp. PCC 6803)
- Tigr03986 family crispr-associated ramp protein (90 kDa, Protein from Synechocystis sp. PCC 6803)
- Water (18 Da, Ligand)
- Self-target rna (19 kDa, RNA from Synechocystis sp. PCC 6803)
CRISPR-Cas type III-D effector complex bound to self-target RNA in a post-cleavage state
Resolution: 3.44 Å
EM Method: Single-particle
Fitted PDBs: 8s9x
Q-score: 0.456
Schwartz EA, Bravo JPK, Ahsan M, Macias LA, McCafferty CL, Dangerfield TL, Walker JN, Brodbelt JS, Palermo G, Fineran PC, Fagerlund RD, Taylor DW
Nat Commun (2024) 15 pp. 3324-3324 [ DOI: doi:10.1038/s41467-024-47506-y Pubmed: 38637512 ]
- Crispr rna (11 kDa, RNA from Synechocystis sp. PCC 6803)
- Self-target rna (19 kDa, RNA from Synechocystis sp. PCC 6803)
- Cas7-2x (56 kDa, Protein from Synechocystis sp. PCC 6803)
- Crispr-cas type iii-d effector complex (332 kDa, Complex from Synechocystis sp. PCC 6803)
- Tigr03984 family crispr-associated protein (21 kDa, Protein from Synechocystis sp. PCC 6803)
- Cas10 (64 kDa, Protein from Synechocystis sp. PCC 6803)
- Cas7-cas5-cas11 (87 kDa, Protein from Synechocystis sp. PCC 6803)
- Tigr03986 family crispr-associated ramp protein (90 kDa, Protein from Synechocystis sp. PCC 6803)
Staphylococcus epidermidis RP62a CRISPR effector subcomplex with non-self target RNA bound
Resolution: 3.49 Å
EM Method: Single-particle
Fitted PDBs: 7uzx
Q-score: 0.371
Smith EM, Ferrell S, Tokars VL, Mondragon A
Structure (2022) 30 pp. 1109-1128.e6 [ Pubmed: 35714601 DOI: doi:10.1016/j.str.2022.05.013 ]
- Crispr system cms protein csm4 (34 kDa, Protein from Staphylococcus epidermidis RP62A)
- Staphylococcus epidermidis rp62a crispr effector subcomplex with target rna bound (Complex from Staphylococcus epidermidis)
- Crispr system cms endoribonuclease csm3 (24 kDa, Protein from Staphylococcus epidermidis RP62A)
- Crispr system single-strand-specific deoxyribonuclease cas10/csm1 (subtype iii-a) (87 kDa, Protein from Staphylococcus epidermidis RP62A)
- Crispr non-self rna target (12 kDa, RNA from Staphylococcus epidermidis RP62A)
- Staphylococcus epidermidis rp62a crispr rna: repeat plus spacer sequence 2 (11 kDa, RNA from Staphylococcus epidermidis RP62A)