Cryo-EM structure of GeoCas9-sgRNA-dsDNA ternary complex
Resolution: 3.08 Å
EM Method: Single-particle
Fitted PDBs: 8jtj
Q-score: 0.421
Shen P, Zhang L, Liu B, Li X, Wang C, Min J, Huang JW, Chen CC, Guo RT
ACS Catal (2024) 14 pp. 13227-13235 [ DOI: doi:10.1021/acscatal.4c03278 ]
- Rna (139-mer) (44 kDa, RNA from Geobacillus stearothermophilus)
- Dna (5'-d(p*gp*gp*gp*cp*gp*cp*gp*ap*a)-3') (2 kDa, DNA from Geobacillus stearothermophilus)
- Type ii crispr rna-guided endonuclease cas9 (130 kDa, Complex from Geobacillus stearothermophilus)
- Crispr-associated endonuclease cas9 (128 kDa, Protein from Geobacillus stearothermophilus)
- Dna (29-mer) (8 kDa, DNA from Geobacillus stearothermophilus)
Cryo-EM structure of GeoCas9-sgRNA binary complex
Resolution: 3.21 Å
EM Method: Single-particle
Fitted PDBs: 8jtr
Q-score: 0.401
Shen P, Zhang L, Liu B, Li X, Wang C, Min J, Huang JW, Chen CC, Guo RT
ACS Catal (2024) 14 pp. 13227-13235 [ DOI: doi:10.1021/acscatal.4c03278 ]
- Crispr-associated endonuclease cas9 (128 kDa, Protein from Geobacillus stearothermophilus)
- Sgrna (139-bp) (44 kDa, RNA from Geobacillus stearothermophilus)
- Type ii crispr rna-guided endonuclease cas9 (130 kDa, Complex from Geobacillus stearothermophilus)
type I-B Cascade bound to a PAM-containing dsDNA target at 3.8 angstrom resolution.
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 8h67
Q-score: 0.307
Lu M, Yu C, Zhang Y, Ju W, Ye Z, Hua C, Mao J, Hu C, Yang Z, Xiao Y
Nat Commun (2024) 15 pp. 4126-4126 [ DOI: doi:10.1038/s41467-024-48598-2 Pubmed: 38750051 ]
- Cryo-em structure of synechocystis sp. pcc6714 cascade bound to a pam-containing dsdna target at 3.8 angstrom resolution. (Complex from Synechocystis sp. PCC 6714)
- Crispr associated protein cas7 (33 kDa, Protein from Synechocystis sp. PCC 6714)
- Crispr associated protein cas11b (14 kDa, Protein from Synechocystis sp. PCC 6714)
- Crispr rna (22 kDa, RNA from synthetic construct)
- Non target dna (2 kDa, DNA from synthetic construct)
- Crispr associated protein cas8 (69 kDa, Protein from Synechocystis sp. PCC 6714)
- Crispr associated protein cas5 (26 kDa, Protein from Synechocystis sp. PCC 6714)
- Target dna (3 kDa, DNA from synthetic construct)
Cryo-EM structure of Synechocystis sp. PCC6714 Cascade at 3.8 angstrom resolution
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 8h7q
Q-score: 0.346
To Be Published
- Target dna (10 kDa, DNA from synthetic construct)
- Crispr rna (11 kDa, RNA from synthetic construct)
- Crispr associated protein cas11b (14 kDa, Protein from Synechocystis sp. PCC 6714)
- Crispr associated protein cas8 (33 kDa, Protein from Synechocystis sp. PCC 6714)
- Type i-b cascade (Complex from Synechocystis sp. PCC 6714)
- Non target dna (3 kDa, DNA from synthetic construct)
- Crispr associated protein cas5 (26 kDa, Protein from Synechocystis sp. PCC 6714)
Cryo-EM structure of LshCas13a-crRNA-anti-tag RNA complex
Resolution: 3.06 Å
EM Method: Single-particle
Fitted PDBs: 7dmq
Q-score: 0.434
Wang B, Zhang T, Yin J, Yu Y, Xu W, Ding J, Patel DJ, Yang H
Mol Cell (2021) 81 pp. 1100- [ DOI: doi:10.1016/j.molcel.2020.12.033 Pubmed: 33472057 ]
- Lshcas13a (Complex)
- Anti-tag target rna (Complex)
- Anti-tag target rna (11 kDa, RNA from Escherichia coli)
- Lsh crrna (Complex)
- Crispr rna (18 kDa, RNA from Leptotrichia shahii)
- Lshcas13a-crrna-anti-tag rna complex (Complex from Leptotrichia shahii)
- Crispr/cas system cas13a (166 kDa, Protein from Leptotrichia shahii)