CryoEM structure of Enterococcus italicus Csm-crRNA (3.2 complex)
Jungfer K, Moravcik S, Garcia-Doval C, Knorlein A, Hall J, Jinek M
Nucleic Acids Res (2025) 53 [ Pubmed: 39817514 DOI: doi:10.1093/nar/gkae1277 ]
- Crispr system cms protein csm2 (16 kDa, Protein from Enterococcus italicus DSM 15952)
- Crispr system cms endoribonuclease csm3 (23 kDa, Protein from Enterococcus italicus DSM 15952)
- Crispr system cms protein csm4 (34 kDa, Protein from Enterococcus italicus DSM 15952)
- Crispr system cms protein csm5 (43 kDa, Protein from Enterococcus italicus DSM 15952)
- Rna (30-mer) (14 kDa, RNA from Enterococcus italicus DSM 15952)
- Binary csm-crrna complex with (csm1)1(csm2)2(csm3)3(csm4)1(csm5)1 stoichiometry (260 kDa, Complex from Enterococcus italicus DSM 15952)
- Crispr system single-strand-specific deoxyribonuclease cas10/csm1 (subtype iii-a) (88 kDa, Protein from Enterococcus italicus DSM 15952)
CryoEM structure of Enterococcus italicus Csm-crRNA (4.3) complex
Jungfer K, Moravcik S, Garcia-Doval C, Knorlein A, Hall J, Jinek M
Nucleic Acids Res (2025) 53 [ Pubmed: 39817514 DOI: doi:10.1093/nar/gkae1277 ]
- Crispr system cms protein csm2 (16 kDa, Protein from Enterococcus italicus DSM 15952)
- Crispr system cms endoribonuclease csm3 (23 kDa, Protein from Enterococcus italicus DSM 15952)
- Crispr system cms protein csm4 (34 kDa, Protein from Enterococcus italicus DSM 15952)
- Crispr system cms protein csm5 (43 kDa, Protein from Enterococcus italicus DSM 15952)
- Rna (35-mer) (14 kDa, RNA from Enterococcus italicus DSM 15952)
- Crispr system single-strand-specific deoxyribonuclease cas10/csm1 (subtype iii-a) (88 kDa, Protein from Enterococcus italicus DSM 15952)
- Binary csm-crrna complex with (csm1)1(csm2)3(csm3)4(csm4)1(csm5)1 stoichiometry (300 kDa, Complex from Enterococcus italicus DSM 15952)
CryoEM structure of Enterococcus italicus Csm-crRNA-CTR1 complex (4.3) bound to AMPNPP
Jungfer K, Moravcik S, Garcia-Doval C, Knorlein A, Hall J, Jinek M
Nucleic Acids Res (2025) 53 [ Pubmed: 39817514 DOI: doi:10.1093/nar/gkae1277 ]
- Crispr system cms protein csm2 (16 kDa, Protein from Enterococcus italicus DSM 15952)
- Covid19 rna (Complex from Severe acute respiratory syndrome coronavirus 2)
- Crispr system cms endoribonuclease csm3 (23 kDa, Protein from Enterococcus italicus DSM 15952)
- Crispr system cms protein csm4 (34 kDa, Protein from Enterococcus italicus DSM 15952)
- Ternary csm-crrna-ctr complex bound to ampnpp in productive state with (csm1)1(csm2)3(csm3)4(csm4)1(csm5)1 stoichiometry (315 kDa, Complex)
- Crispr system cms protein csm5 (43 kDa, Protein from Enterococcus italicus DSM 15952)
- Ctr (15 kDa, RNA from Severe acute respiratory syndrome coronavirus 2)
- Rna (35-mer) (14 kDa, RNA from Enterococcus italicus DSM 15952)
- Enterococcus italicus csm-crrna-ctr1 complex (4.3) (Complex from Enterococcus italicus DSM 15952)
- 5'-o-[(s)-hydroxy{[(s)-hydroxy(phosphonooxy)phosphoryl]amino}phosphoryl]adenosine (506 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Crispr system single-strand-specific deoxyribonuclease cas10/csm1 (subtype iii-a) (88 kDa, Protein from Enterococcus italicus DSM 15952)
CryoEM structure of Enterococcus italicus Csm-crRNA-CTR1 complex bound to pNppA3 and AMPNPP
Jungfer K, Moravcik S, Garcia-Doval C, Knorlein A, Hall J, Jinek M
Nucleic Acids Res (2025) 53 [ Pubmed: 39817514 DOI: doi:10.1093/nar/gkae1277 ]
- Enterococcus italicus csm-crrna-ctr1 complex (Complex from Enterococcus italicus DSM 15952)
- Covid19 rna (Complex from Severe acute respiratory syndrome coronavirus 2)
- Crispr system cms protein csm5 (43 kDa, Protein from Enterococcus italicus DSM 15952)
- Crispr system cms protein csm2 (16 kDa, Protein from Enterococcus italicus DSM 15952)
- Crispr system cms endoribonuclease csm3 (23 kDa, Protein from Enterococcus italicus DSM 15952)
- Crispr system cms protein csm4 (34 kDa, Protein from Enterococcus italicus DSM 15952)
- Ctr (15 kDa, RNA from Severe acute respiratory syndrome coronavirus 2)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Adenosine-5'-[(beta,gamma)-imido]triphosphate-adenosine-monophosphate-adenosine-monophosphate (1 kDa, Ligand)
- Crrna (14 kDa, RNA from Enterococcus italicus DSM 15952)
- Magnesium ion (24 Da, Ligand)
- Crispr system single-strand-specific deoxyribonuclease cas10/csm1 (subtype iii-a) (88 kDa, Protein from Enterococcus italicus DSM 15952)
- Ternary csm-crrna-ctr complex bound to pnppa3 and amppnp in productive state with (csm1)1(csm2)2(csm3)3(csm4)1(csm5)1 stoichiometry (275 kDa, Complex)
Cryo-EM structure of CRISPR-Csm effector complex from Mycobacterium canettii
Resolution: 3.73 Å
EM Method: Single-particle
Fitted PDBs: 8wfx
Q-score: 0.39
Zhang H, Shi M, Ma X, Liu M, Wang N, Lu Q, Li Z, Zhao Y, Zhao H, Chen H, Zhang H, Jiang T, Ouyang S, Huo Y, Bi L
Int J Biol Macromol (2024) 260 pp. 129331-129331 [ DOI: doi:10.1016/j.ijbiomac.2024.129331 Pubmed: 38218299 ]
- Crispr system cms protein csm2 (15 kDa, Protein from Mycobacterium canettii)
- Csm effector complex (Complex from Mycobacterium canettii)
- Crispr system single-strand-specific deoxyribonuclease cas10/csm1 (subtype iii-a) (91 kDa, Protein from Mycobacterium canettii)
- Crispr system cms protein csm4 (32 kDa, Protein from Mycobacterium canettii)
- Crispr system cms endoribonuclease csm3 (25 kDa, Protein from Mycobacterium canettii)
- Rna (50-mer) (16 kDa, RNA from Mycobacterium canettii)
- Crispr system cms protein csm5 (42 kDa, Protein from Mycobacterium canettii)
The structure of S. epidermidis Cas10-Csm bound to target RNA
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 8do6
Q-score: 0.463
Paraan M, Nasef M, Chou-Zheng L, Khweis SA, Schoeffler AJ, Hatoum-Aslan A, Stagg SM, Dunkle JA
PLoS One (2023) 18 pp. e0287461-e0287461 [ DOI: doi:10.1371/journal.pone.0287461 Pubmed: 37352230 ]
- Cas10-csm complex bound to target rna (300 kDa, Complex from Staphylococcus epidermidis RP62A)
- Crispr system cms protein csm4 (34 kDa, Protein from Staphylococcus epidermidis RP62A)
- Crispr system cms endoribonuclease csm3 (24 kDa, Protein from Staphylococcus epidermidis RP62A)
- Target rna (13 kDa, RNA from Staphylococcus epidermidis RP62A)
- Crispr system cms protein csm2 (16 kDa, Protein from Staphylococcus epidermidis RP62A)
- Crispr system cms protein csm5 (39 kDa, Protein from Staphylococcus epidermidis RP62A)
- Crrna (11 kDa, RNA from Staphylococcus epidermidis RP62A)