Sasnauskas G, Gaizauskaite U, Tamulaitiene G
To Be Published
- Integration target, chain j (14 kDa, DNA from synthetic construct)
- Prespacer dna, chain h (7 kDa, DNA from synthetic construct)
- Magnesium ion (24 Da, Ligand)
- Cas1-cas2 crispr integrase bound to prespacer and target dna, streptococcus thermophilus dgcc 7710 crispr3 system (Complex from Streptococcus thermophilus DGCC 7710)
- Crispr-associated endonuclease cas1 (35 kDa, Protein from Streptococcus thermophilus DGCC 7710)
- Integration target, chain i (14 kDa, DNA from synthetic construct)
- Prespacer dna, chain g (8 kDa, DNA from synthetic construct)
- Crispr-associated endoribonuclease cas2 (13 kDa, Protein from Streptococcus thermophilus DGCC 7710)
Resolution: 3.17 Å
EM Method: Single-particle
Fitted PDBs: 8pk1
Q-score: 0.463
Sasnauskas G, Gaizauskaite U, Tamulaitiene G
To Be Published
- Magnesium ion (24 Da, Ligand)
- Crispr-associated endonuclease cas1 (35 kDa, Protein from Streptococcus thermophilus DGCC 7710)
- Chains: g,j (7 kDa, DNA from synthetic construct)
- Cas1-cas2 crispr integrase-prespacer dna complex (Complex from Streptococcus thermophilus DGCC 7710)
- Chains: h,k (8 kDa, DNA from synthetic construct)
- Crispr-associated endoribonuclease cas2 (13 kDa, Protein from Streptococcus thermophilus DGCC 7710)
Streptococcus thermophilus Cas1-Cas2- prespacer ternary complex
Resolution: 3.09 Å
EM Method: Single-particle
Fitted PDBs: 8hi1
Q-score: 0.473
Tang D, Jia T, Luo Y, Mou B, Cheng J, Qi S, Yao S, Su Z, Yu Y, Chen Q
Innovation (NY) (2023) 4 pp. 100495-100495 [ DOI: doi:10.1016/j.xinn.2023.100495 Pubmed: 37663930 ]
- Streptococcus thermophilus cas1-cas2- prespacer ternary complex (Complex from Streptococcus thermophilus DGCC 7710)
- Dna (31-mer) (11 kDa, DNA from Phage #D)
- Type i-e crispr-associated endoribonuclease cas2 (13 kDa, Protein from Streptococcus thermophilus DGCC 7710)
- Crispr-associated endonuclease cas1 (35 kDa, Protein from Streptococcus thermophilus DGCC 7710)
- Dna (26-mer) (11 kDa, DNA from Phage #D)
Cas1-Cas2/3 integrase and IHF bound to CRISPR leader, repeat and foreign DNA
Resolution: 3.48 Å
EM Method: Single-particle
Fitted PDBs: 8flj
Q-score: 0.35
Santiago-Frangos A, Henriques WS, Wiegand T, Gauvin CC, Buyukyoruk M, Graham AB, Wilkinson RA, Triem L, Neselu K, Eng ET, Lander GC, Wiedenheft B
Nat Struct Mol Biol (2023) 30 pp. 1675-1685 [ Pubmed: 37710013 DOI: doi:10.1038/s41594-023-01097-2 ]
- Cas1-cas2/3 heterohexameric integrase (Complex from Pseudomonas aeruginosa PA14)
- Crispr leader and repeat, anti-sense strand of dna (52 kDa, DNA from Pseudomonas aeruginosa PA14)
- Prespacer, anti-sense strand of dna (8 kDa, DNA from synthetic construct)
- Integration host factor subunit beta (10 kDa, Protein from Pseudomonas aeruginosa PA14)
- Crispr-associated endonuclease cas1 (38 kDa, Protein from Pseudomonas aeruginosa PA14)
- Crispr repeat and prespacer, sense strand of dna (18 kDa, DNA from synthetic construct)
- Integration host factor subunit alpha (11 kDa, Protein from Pseudomonas aeruginosa PA14)
- Crispr leader, sense strand of dna (42 kDa, DNA from Pseudomonas aeruginosa PA14)
- Crispr-associated nuclease/helicase cas3 subtype i-f/ypest (121 kDa, Protein from Pseudomonas aeruginosa PA14)
- Ihf heterodimer (Complex from Pseudomonas aeruginosa PA14)
- Crispr leader, repeat, and prespacer dna annealed to mimic a half-site integration intermediate. (Complex)
- Cas1-cas2/3 integrase and ihf bound to crispr leader, repeat and foreign dna (122 kDa, Complex)
Type I-C Cas4-Cas1-Cas2 complex bound to a PAM/PAM prespacer
Resolution: 3.49 Å
EM Method: Single-particle
Fitted PDBs: 8d3l
Q-score: 0.444
Dhingra Y, Suresh SK, Juneja P, Sashital DG
Mol Cell (2022) 82 pp. 4353- [ Pubmed: 36272411 DOI: doi:10.1016/j.molcel.2022.09.030 ]
- Pam/pam strand 1 (9 kDa, DNA from synthetic construct)
- Crispr-associated endonuclease cas1 (39 kDa, Protein from Alkalihalobacillus halodurans C-125)
- Crispr-associated endonuclease cas2 (11 kDa, Protein from Alkalihalobacillus halodurans C-125)
- Type i-c cas4-cas1-cas2 complex with a pam/pam substrate (Complex from Alkalihalobacillus halodurans C-125)
- Manganese (ii) ion (54 Da, Ligand)
- Water (18 Da, Ligand)
- Iron/sulfur cluster (351 Da, Ligand)
- Crispr-associated exonuclease cas4 (25 kDa, Protein from Alkalihalobacillus halodurans C-125)
- Pam/pam strand 2 (10 kDa, DNA from synthetic construct)
Type I-C Cas4-Cas1-Cas2 complex bound to a PAM/Processed prespacer
Resolution: 3.41 Å
EM Method: Single-particle
Fitted PDBs: 8d3m
Q-score: 0.449
Dhingra Y, Suresh SK, Juneja P, Sashital DG
Mol Cell (2022) 82 pp. 4353- [ Pubmed: 36272411 DOI: doi:10.1016/j.molcel.2022.09.030 ]
- Crispr-associated endonuclease cas1 (39 kDa, Protein from Alkalihalobacillus halodurans C-125)
- Crispr-associated endonuclease cas2 (11 kDa, Protein from Alkalihalobacillus halodurans C-125)
- Pam/processed strand 2 (10 kDa, DNA from synthetic construct)
- Manganese (ii) ion (54 Da, Ligand)
- Type i-c cas4-cas1-cas2 complex with a pam/processed substrate (Complex from Alkalihalobacillus halodurans C-125)
- Pam/processed strand 1 (9 kDa, DNA from synthetic construct)
- Iron/sulfur cluster (351 Da, Ligand)
- Crispr-associated exonuclease cas4 (25 kDa, Protein from Alkalihalobacillus halodurans C-125)
Type I-C Cas4-Cas1-Cas2 complex bound to half-site integration intermediate (HSI)
Resolution: 4.26 Å
EM Method: Single-particle
Fitted PDBs: 8d3p
Q-score: 0.278
Dhingra Y, Suresh SK, Juneja P, Sashital DG
Mol Cell (2022) 82 pp. 4353- [ Pubmed: 36272411 DOI: doi:10.1016/j.molcel.2022.09.030 ]
- Manganese (ii) ion (54 Da, Ligand)
- Crispr-associated endonuclease cas1 (39 kDa, Protein from Alkalihalobacillus halodurans C-125)
- Type i-c cas4-cas1-cas2 complex with a pam/processed substrate (Complex from Alkalihalobacillus halodurans C-125)
- Hsi strand 2- integrated prespacer strand plus repeat (17 kDa, DNA from synthetic construct)
- Iron/sulfur cluster (351 Da, Ligand)
- Hsi strand 4 top leader strand (3 kDa, DNA from synthetic construct)
- Crispr-associated endonuclease cas2 (11 kDa, Protein from Alkalihalobacillus halodurans C-125)
- Crispr-associated exonuclease cas4 (25 kDa, Protein from Alkalihalobacillus halodurans C-125)
- Hsi strand 2 (11 kDa, DNA from synthetic construct)
- Hsi strand 3 bottom strand leader-repeat (12 kDa, DNA from synthetic construct)
Type I-C Cas4-Cas1-Cas2 complex bound to a PAM/NoPAM prespacer
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 8d3q
Q-score: 0.257
Dhingra Y, Suresh SK, Juneja P, Sashital DG
Mol Cell (2022) 82 pp. 4353- [ Pubmed: 36272411 DOI: doi:10.1016/j.molcel.2022.09.030 ]
- Crispr-associated endonuclease cas1 (39 kDa, Protein from Alkalihalobacillus halodurans C-125)
- Crispr-associated endonuclease cas2 (11 kDa, Protein from Alkalihalobacillus halodurans C-125)
- Manganese (ii) ion (54 Da, Ligand)
- Type i-c cas4-cas1-cas2 complex with a pam/processed substrate (Complex from Alkalihalobacillus halodurans C-125)
- Iron/sulfur cluster (351 Da, Ligand)
- Crispr-associated exonuclease cas4 (25 kDa, Protein from Alkalihalobacillus halodurans C-125)
- Pam/nopam strand 2 (8 kDa, DNA from synthetic construct)
- Pam/nopam strand 1 (9 kDa, DNA from synthetic construct)
Cas1-Cas2-Csn2-DNA complex from the Type II-A CRISPR-Cas system
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 6qxf
Q-score: 0.309
Wilkinson M, Drabavicius G, Silanskas A, Gasiunas G, Siksnys V, Wigley DB
Mol Cell (2019) 75 pp. 90-101.e5 [ Pubmed: 31080012 DOI: doi:10.1016/j.molcel.2019.04.020 ]
- Cas1-cas2-csn2-dna monomer complex (530 kDa, Complex)
- Dna (25-mer) (7 kDa, DNA from Escherichia coli BL21(DE3))
- Crispr-associated endoribonuclease cas2 (13 kDa, Protein from Streptococcus thermophilus)
- Crispr-associated protein csn2 (25 kDa, Protein from Streptococcus thermophilus)
- Crispr-associated endonuclease cas1 (35 kDa, Protein from Streptococcus thermophilus)
- Dna (Complex from Escherichia coli BL21(DE3))
- Calcium ion (40 Da, Ligand)
- Cas1-cas2-csn2 (Complex from Streptococcus thermophilus)
- Dna (25-mer) (7 kDa, DNA from Escherichia coli BL21(DE3))
Cas1-Cas2-Csn2-DNA dimer complex from the Type II-A CRISPR-Cas system
Resolution: 8.9 Å
EM Method: Single-particle
Fitted PDBs: 6qxt
Q-score: 0.197
Wilkinson M, Drabavicius G, Silanskas A, Gasiunas G, Siksnys V, Wigley DB
Mol Cell (2019) 75 pp. 90-101.e5 [ Pubmed: 31080012 DOI: doi:10.1016/j.molcel.2019.04.020 ]
- Dna (64-mer) (20 kDa, DNA from Escherichia coli BL21(DE3))
- Cas1-cas2-csn2-dna monomer complex (1 MDa, Complex)
- Crispr-associated endoribonuclease cas2 (13 kDa, Protein from Streptococcus thermophilus)
- Crispr-associated protein csn2 (25 kDa, Protein from Streptococcus thermophilus)
- Crispr-associated endonuclease cas1 (35 kDa, Protein from Streptococcus thermophilus)
- Dna (64-mer) (19 kDa, DNA from Escherichia coli BL21(DE3))
- Dna (Complex from Escherichia coli BL21(DE3))
- Calcium ion (40 Da, Ligand)
- Cas1-cas2-csn2 (Complex from Streptococcus thermophilus)