Cas1-Cas2/3 integrase and IHF bound to CRISPR leader, repeat and foreign DNA
Resolution: 3.48 Å
EM Method: Single-particle
Fitted PDBs: 8flj
Q-score: 0.35
Santiago-Frangos A, Henriques WS, Wiegand T, Gauvin CC, Buyukyoruk M, Graham AB, Wilkinson RA, Triem L, Neselu K, Eng ET, Lander GC, Wiedenheft B
Nat Struct Mol Biol (2023) 30 pp. 1675-1685 [ DOI: doi:10.1038/s41594-023-01097-2 Pubmed: 37710013 ]
- Crispr repeat and prespacer, sense strand of dna (18 kDa, DNA from synthetic construct)
- Cas1-cas2/3 heterohexameric integrase (Complex from Pseudomonas aeruginosa PA14)
- Crispr-associated nuclease/helicase cas3 subtype i-f/ypest (121 kDa, Protein from Pseudomonas aeruginosa PA14)
- Integration host factor subunit alpha (11 kDa, Protein from Pseudomonas aeruginosa PA14)
- Crispr leader, repeat, and prespacer dna annealed to mimic a half-site integration intermediate. (Complex)
- Ihf heterodimer (Complex from Pseudomonas aeruginosa PA14)
- Cas1-cas2/3 integrase and ihf bound to crispr leader, repeat and foreign dna (122 kDa, Complex)
- Integration host factor subunit beta (10 kDa, Protein from Pseudomonas aeruginosa PA14)
- Crispr leader, sense strand of dna (42 kDa, DNA from Pseudomonas aeruginosa PA14)
- Crispr leader and repeat, anti-sense strand of dna (52 kDa, DNA from Pseudomonas aeruginosa PA14)
- Crispr-associated endonuclease cas1 (38 kDa, Protein from Pseudomonas aeruginosa PA14)
- Prespacer, anti-sense strand of dna (8 kDa, DNA from synthetic construct)
Cas1-Cas2-Csn2-DNA complex from the Type II-A CRISPR-Cas system
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 6qxf
Q-score: 0.309
Wilkinson M, Drabavicius G, Silanskas A, Gasiunas G, Siksnys V, Wigley DB
Mol Cell (2019) 75 pp. 90-101.e5 [ Pubmed: 31080012 DOI: doi:10.1016/j.molcel.2019.04.020 ]
- Dna (25-mer) (7 kDa, DNA from Escherichia coli BL21(DE3))
- Calcium ion (40 Da, Ligand)
- Cas1-cas2-csn2-dna monomer complex (530 kDa, Complex)
- Dna (Complex from Escherichia coli BL21(DE3))
- Crispr-associated endonuclease cas1 (35 kDa, Protein from Streptococcus thermophilus)
- Cas1-cas2-csn2 (Complex from Streptococcus thermophilus)
- Dna (25-mer) (7 kDa, DNA from Escherichia coli BL21(DE3))
- Crispr-associated endoribonuclease cas2 (13 kDa, Protein from Streptococcus thermophilus)
- Crispr-associated protein csn2 (25 kDa, Protein from Streptococcus thermophilus)
Cas1-Cas2-Csn2-DNA dimer complex from the Type II-A CRISPR-Cas system
Resolution: 8.9 Å
EM Method: Single-particle
Fitted PDBs: 6qxt
Q-score: 0.197
Wilkinson M, Drabavicius G, Silanskas A, Gasiunas G, Siksnys V, Wigley DB
Mol Cell (2019) 75 pp. 90-101.e5 [ Pubmed: 31080012 DOI: doi:10.1016/j.molcel.2019.04.020 ]
- Calcium ion (40 Da, Ligand)
- Cas1-cas2-csn2-dna monomer complex (1 MDa, Complex)
- Dna (Complex from Escherichia coli BL21(DE3))
- Dna (64-mer) (19 kDa, DNA from Escherichia coli BL21(DE3))
- Crispr-associated endonuclease cas1 (35 kDa, Protein from Streptococcus thermophilus)
- Cas1-cas2-csn2 (Complex from Streptococcus thermophilus)
- Dna (64-mer) (20 kDa, DNA from Escherichia coli BL21(DE3))
- Crispr-associated endoribonuclease cas2 (13 kDa, Protein from Streptococcus thermophilus)
- Crispr-associated protein csn2 (25 kDa, Protein from Streptococcus thermophilus)
Segment of the Cas1-Cas2-Csn2-DNA filament complex from the Type II-A CRISPR-Cas system
Resolution: 9.1 Å
EM Method: Single-particle
Fitted PDBs: 6qy3
Q-score: 0.163
Wilkinson M, Drabavicius G, Silanskas A, Gasiunas G, Siksnys V, Wigley DB
Mol Cell (2019) 75 pp. 90-101.e5 [ Pubmed: 31080012 DOI: doi:10.1016/j.molcel.2019.04.020 ]
- Calcium ion (40 Da, Ligand)
- Minimal unit of the cas1-cas2-csn2 (Complex from Streptococcus thermophilus)
- Cas1-cas2-csn2-dna monomer complex (530 kDa, Complex)
- Dna (Complex from Escherichia coli BL21(DE3))
- Dna (66-mer) (20 kDa, DNA from Escherichia coli BL21(DE3))
- Crispr-associated endonuclease cas1 (35 kDa, Protein from Streptococcus thermophilus)
- Dna (66-mer) (20 kDa, DNA from Escherichia coli BL21(DE3))
- Crispr-associated endoribonuclease cas2 (13 kDa, Protein from Streptococcus thermophilus)
- Crispr-associated protein csn2 (25 kDa, Protein from Streptococcus thermophilus)
Cas1-Cas2-IHF-DNA holo-complex
Resolution: 3.64 Å
EM Method: Single-particle
Fitted PDBs: 5wfe
Q-score: 0.334
Wright AV, Liu JJ, Knott GJ, Doxzen KW, Nogales E, Doudna JA
Science (2017) 357 pp. 1113-1118 [ DOI: doi:10.1126/science.aao0679 Pubmed: 28729350 ]
- Dna (61-mer) (19 kDa, DNA from synthetic construct)
- Cas1-cas2-ihf-dna holo complex (Complex from Escherichia coli)
- Crispr-associated endonuclease cas1 (33 kDa, Protein from Escherichia coli K-12)
- Crispr-associated endoribonuclease cas2 (11 kDa, Protein from Escherichia coli K-12)
- Integration host factor subunit alpha (11 kDa, Protein from Escherichia coli S88)
- Integration host factor subunit beta (10 kDa, Protein from Escherichia coli S88)
- Dna (28-mer) (8 kDa, DNA from synthetic construct)
- Dna (76-mer) (29 kDa, DNA from synthetic construct)
- Dna (45-mer) (18 kDa, DNA from synthetic construct)