Cryo-EM structure of Cascade-DNA (P23) complex in type I-B CAST system
Resolution: 2.83 Å
EM Method: Single-particle
Fitted PDBs: 8fcj
Q-score: 0.534
Wang S, Gabel C, Siddique R, Klose T, Chang L
Cell (2023) 186 pp. 4204-4215.e19 [ Pubmed: 37557170 DOI: doi:10.1016/j.cell.2023.07.010 ]
- Type i-b crispr-associated protein cas5 (24 kDa, Protein from Nostoc sp. 'Peltigera membranacea cyanobiont' 210A)
- Non-target dna strand (20 kDa, DNA from synthetic construct)
- Target dna strand (19 kDa, DNA from synthetic construct)
- Type i-b crispr-associated protein cas11 (16 kDa, Protein from Nostoc sp. 'Peltigera membranacea cyanobiont' 210A)
- Type i-b crispr-associated protein cas7 (37 kDa, Protein from Nostoc sp. 'Peltigera membranacea cyanobiont' 210A)
- Rna (22 kDa, RNA from synthetic construct)
- Type i-b crispr-associated protein cas6 (24 kDa, Protein from Nostoc sp. 'Peltigera membranacea cyanobiont' 210A)
- Ternary complex of cascade proteins, crrna and target dna with 23 bp recognized. (Complex from Peltigera membranacea)
- Type i-b crispr-associated protein cas8 (63 kDa, Protein from Nostoc sp. 'Peltigera membranacea cyanobiont' 210A)
Cryo-EM structure of Cascade-DNA-TniQ-TnsC complex in type I-B CAST system
Resolution: 3.19 Å
EM Method: Single-particle
Fitted PDBs: 8fcu
Q-score: 0.393
Wang S, Gabel C, Siddique R, Klose T, Chang L
Cell (2023) 186 pp. 4204-4215.e19 [ DOI: doi:10.1016/j.cell.2023.07.010 Pubmed: 37557170 ]
- Rna (22 kDa, RNA from synthetic construct)
- Type i-b crispr-associated protein cas11 (16 kDa, Protein from Nostoc sp. 'Peltigera membranacea cyanobiont' 210A)
- Type i-b crispr-associated protein cas6 (24 kDa, Protein from Nostoc sp. 'Peltigera membranacea cyanobiont' 210A)
- Type i-b crispr-associated protein cas5 (24 kDa, Protein from Nostoc sp. 'Peltigera membranacea cyanobiont' 210A)
- Non-target dna strand (26 kDa, DNA from synthetic construct)
- Type i-b crispr-associated protein cas7 (37 kDa, Protein from Nostoc sp. 'Peltigera membranacea cyanobiont' 210A)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Tnsc (43 kDa, Protein from Nostoc sp. 'Peltigera membranacea cyanobiont' 210A)
- Target dna strand (26 kDa, DNA from synthetic construct)
- Tniq (37 kDa, Protein from Nostoc sp. 'Peltigera membranacea cyanobiont' 210A)
- Cascade-dna-tniq-tnsc complex (Complex from Peltigera membranacea)
- Type i-myxan crispr-associated cas8a1/cmx1 (63 kDa, Protein from Nostoc sp. 'Peltigera membranacea cyanobiont' 210A)
Cryo-EM structure of Cascade complex in type I-B CAST system
Resolution: 3.65 Å
EM Method: Single-particle
Fitted PDBs: 8fd2
Q-score: 0.23
Wang S, Gabel C, Siddique R, Klose T, Chang L
Cell (2023) 186 pp. 4204-4215.e19 [ DOI: doi:10.1016/j.cell.2023.07.010 Pubmed: 37557170 ]
- Rna (22 kDa, RNA from synthetic construct)
- Type i-b crispr-associated protein cas11 (16 kDa, Protein from Nostoc sp. 'Peltigera membranacea cyanobiont' 210A)
- Type i-b crispr-associated protein cas6 (24 kDa, Protein from Nostoc sp. 'Peltigera membranacea cyanobiont' 210A)
- Type i-b crispr-associated protein cas7 (37 kDa, Protein from Nostoc sp. 'Peltigera membranacea cyanobiont' 210A)
- A type i-b crispr-associated protein cas5 (24 kDa, Protein from Nostoc sp. 'Peltigera membranacea cyanobiont' 210A)
- Cascade complex in type i-b cast system (Complex from Peltigera membranacea)
- Type i-b crispr-associated protein cas8 (63 kDa, Protein from Nostoc sp. 'Peltigera membranacea cyanobiont' 210A)
Cryo-EM structure of Cascade-PAM complex in type I-B CAST system
Resolution: 3.12 Å
EM Method: Single-particle
Fitted PDBs: 8fd3
Q-score: 0.347
Wang S, Gabel C, Siddique R, Klose T, Chang L
Cell (2023) 186 pp. 4204-4215.e19 [ DOI: doi:10.1016/j.cell.2023.07.010 Pubmed: 37557170 ]
- Rna (22 kDa, RNA from synthetic construct)
- Ternary complex of cascade proteins, crrna and pam only dna (Complex from Peltigera membranacea)
- Type i-b crispr-associated protein cas11 (16 kDa, Protein from Nostoc sp. 'Peltigera membranacea cyanobiont' 210A)
- Type i-b crispr-associated protein cas6 (24 kDa, Protein from Nostoc sp. 'Peltigera membranacea cyanobiont' 210A)
- Type i-b crispr-associated protein cas5 (24 kDa, Protein from Nostoc sp. 'Peltigera membranacea cyanobiont' 210A)
- Type i-b crispr-associated protein cas7 (37 kDa, Protein from Nostoc sp. 'Peltigera membranacea cyanobiont' 210A)
- Target dna strand (3 kDa, DNA from synthetic construct)
- Non-target dna strand (13 kDa, DNA from synthetic construct)
- Type i-myxan crispr-associated cas8a1/cmx1 (63 kDa, Protein from Nostoc sp. 'Peltigera membranacea cyanobiont' 210A)
Cryo-EM structure of Cascade-DNA-TniQ-TnsC complex (composite) in type I-B CAST system
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 8ff4
Q-score: 0.417
Wang S, Gabel C, Siddique R, Klose T, Chang L
Cell (2023) 186 pp. 4204-4215.e19 [ DOI: doi:10.1016/j.cell.2023.07.010 Pubmed: 37557170 ]
- Rna (22 kDa, RNA from synthetic construct)
- Type i-b crispr-associated protein cas11 (16 kDa, Protein from Nostoc sp. 'Peltigera membranacea cyanobiont' 210A)
- Type i-b crispr-associated protein cas6 (24 kDa, Protein from Nostoc sp. 'Peltigera membranacea cyanobiont' 210A)
- Type i-b crispr-associated protein cas5 (24 kDa, Protein from Nostoc sp. 'Peltigera membranacea cyanobiont' 210A)
- Non-target dna strand (26 kDa, DNA from synthetic construct)
- Type i-b crispr-associated protein cas7 (37 kDa, Protein from Nostoc sp. 'Peltigera membranacea cyanobiont' 210A)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Type i-b crispr-associated protein cas8 (63 kDa, Protein from Nostoc sp. 'Peltigera membranacea cyanobiont' 210A)
- Tnsc (43 kDa, Protein from Nostoc sp. 'Peltigera membranacea cyanobiont' 210A)
- Target dna strand (26 kDa, DNA from synthetic construct)
- Tniq (37 kDa, Protein from Nostoc sp. 'Peltigera membranacea cyanobiont' 210A)
- Structure of cascade-dna-tniq-tnsc complex (composite) in type i-b cast system (Complex from Peltigera membranacea)
Cryo-EM structure of Cascade-DNA-fullRloop in type I-B CAST system
Resolution: 3.13 Å
EM Method: Single-particle
Fitted PDBs: 8ff5
Q-score: 0.372
Wang S, Gabel C, Siddique R, Klose T, Chang L
Cell (2023) 186 pp. 4204-4215.e19 [ DOI: doi:10.1016/j.cell.2023.07.010 Pubmed: 37557170 ]
- Ternary complex of cascade proteins, crrna and target dna with the complete r loop formed. (Complex from Peltigera membranacea)
- Nostoc sp. 'peltigera membranacea cyanobiont' 210a (24 kDa, Protein from Peltigera membranacea)
- Rna (22 kDa, RNA from synthetic construct)
- Target dna strand (19 kDa, DNA from synthetic construct)
- Non-target dna strand (20 kDa, DNA from synthetic construct)
- Type i-b crispr-associated protein cas11 (16 kDa, Protein from Nostoc sp. 'Peltigera membranacea cyanobiont' 210A)
- Type i-b crispr-associated protein cas6 (24 kDa, Protein from Nostoc sp. 'Peltigera membranacea cyanobiont' 210A)
- Type i-b crispr-associated protein cas7 (37 kDa, Protein from Nostoc sp. 'Peltigera membranacea cyanobiont' 210A)
- Type i-b crispr-associated protein cas8 (63 kDa, Protein from Nostoc sp. 'Peltigera membranacea cyanobiont' 210A)
CryoEM structure of Cascade-TniQ-dsDNA complex
Resolution: 3.18 Å
EM Method: Single-particle
Fitted PDBs: 6lnb
Q-score: 0.441
Cell Res (2020) 30 pp. 185-187 [ Pubmed: 31913359 DOI: doi:10.1038/s41422-020-0274-0 ]
- Target dna strand (51-mer) (15 kDa, DNA from Escherichia coli)
- Cryoem structure of cascade-tniq-dsdna (Complex from Vibrio cholerae)
- Crispr-associated protein cas6 (23 kDa, Protein from Vibrio cholerae)
- Transposition protein tniq (45 kDa, Protein from Vibrio cholerae)
- Crispr-associated protein cas8 (72 kDa, Protein from Vibrio cholerae)
- Crispr-associated protein cas7 (40 kDa, Protein from Vibrio cholerae)
- Crispr rna (60-mer) (19 kDa, RNA from Vibrio cholerae)
- Non-target dna strand (51-mer) (15 kDa, DNA from Escherichia coli)
CryoEM structure of Cascade-TniQ complex
Resolution: 3.21 Å
EM Method: Single-particle
Fitted PDBs: 6lnc
Q-score: 0.433
Cell Res (2020) 30 pp. 185-187 [ Pubmed: 31913359 DOI: doi:10.1038/s41422-020-0274-0 ]
- Crispr-associated protein cas6 (23 kDa, Protein from Vibrio cholerae)
- Crispr-associated protein cas8 (72 kDa, Protein from Vibrio cholerae)
- Crispr rna (60-mer) (19 kDa, RNA from Vibrio cholerae)
- Crispr-associated protein cas7 (40 kDa, Protein from Vibrio cholerae)
- Transposition protein tniq (45 kDa, Protein from Vibrio cholerae)
- Cryoem structure of cascade-tniq complex (Complex from Vibrio cholerae)