CryoEM structure of Enterococcus italicus Csm-crRNA-CTR1 complex bound to pNppA3 and AMPNPP
Jungfer K, Moravcik S, Garcia-Doval C, Knorlein A, Hall J, Jinek M
Nucleic Acids Res (2025) 53 [ Pubmed: 39817514 DOI: doi:10.1093/nar/gkae1277 ]
- Crispr system cms protein csm4 (34 kDa, Protein from Enterococcus italicus DSM 15952)
- Crispr system cms protein csm5 (43 kDa, Protein from Enterococcus italicus DSM 15952)
- Adenosine-5'-[(beta,gamma)-imido]triphosphate-adenosine-monophosphate-adenosine-monophosphate (1 kDa, Ligand)
- Enterococcus italicus csm-crrna-ctr1 complex (Complex from Enterococcus italicus DSM 15952)
- Crrna (14 kDa, RNA from Enterococcus italicus DSM 15952)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Ternary csm-crrna-ctr complex bound to pnppa3 and amppnp in productive state with (csm1)1(csm2)2(csm3)3(csm4)1(csm5)1 stoichiometry (275 kDa, Complex)
- Magnesium ion (24 Da, Ligand)
- Crispr system single-strand-specific deoxyribonuclease cas10/csm1 (subtype iii-a) (88 kDa, Protein from Enterococcus italicus DSM 15952)
- Crispr system cms protein csm2 (16 kDa, Protein from Enterococcus italicus DSM 15952)
- Ctr (15 kDa, RNA from Severe acute respiratory syndrome coronavirus 2)
- Crispr system cms endoribonuclease csm3 (23 kDa, Protein from Enterococcus italicus DSM 15952)
- Covid19 rna (Complex from Severe acute respiratory syndrome coronavirus 2)
Cryo-EM structure of type III-A Csm-CTR1 complex, AMPPNP bound
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 6ifk
Q-score: 0.511
You L, Ma J, Wang J, Artamonova D, Wang M, Liu L, Xiang H, Severinov K, Zhang X, Wang Y
Cell (2019) 176 pp. 239-253.e16 [ DOI: doi:10.1016/j.cell.2018.10.052 Pubmed: 30503210 ]
- Type iii-a crispr-associated ramp protein csm3 (24 kDa, Protein from Streptococcus thermophilus ND03)
- Ctr1 (13 kDa, RNA from Streptococcus thermophilus ND03)
- Magnesium ion (24 Da, Ligand)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Type iii-a crispr-associated protein csm1 (86 kDa, Protein from Streptococcus thermophilus ND03)
- Type iii-a crispr-associated ramp protein csm5 (41 kDa, Protein from Streptococcus thermophilus ND03)
- Type iii-a crispr-associated ramp protein csm4 (33 kDa, Protein from Streptococcus thermophilus ND03)
- Type iii-a crispr-associated protein csm2 (14 kDa, Protein from Streptococcus thermophilus ND03)
- Zinc ion (65 Da, Ligand)
- Csm-ctr1 complex, amppnp bound (290 kDa, Complex from Streptococcus thermophilus ND03)
- Crrna (10 kDa, RNA from Streptococcus thermophilus ND03)
Type III-A Csm complex, Cryo-EM structure of Csm-CTR1
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 6ify
Q-score: 0.379
You L, Ma J, Wang J, Artamonova D, Wang M, Liu L, Xiang H, Severinov K, Zhang X, Wang Y
Cell (2019) 176 pp. 239-253.e16 [ DOI: doi:10.1016/j.cell.2018.10.052 Pubmed: 30503210 ]
- Type iii-a crispr-associated ramp protein csm3 (24 kDa, Protein from Streptococcus thermophilus ND03)
- Ctr1 (13 kDa, RNA from Streptococcus thermophilus ND03)
- Crrna (11 kDa, RNA from Streptococcus thermophilus ND03)
- Csm1 (Complex from Streptococcus thermophilus ND03)
- Csm-ctr1 complex (87 kDa, Complex from Streptococcus thermophilus ND03)
- Type iii-a crispr-associated protein csm2 (14 kDa, Protein from Streptococcus thermophilus ND03)
- Zinc ion (65 Da, Ligand)
- Type iii-a crispr-associated protein csm1 (86 kDa, Protein from Streptococcus thermophilus ND03)
- Type iii-a crispr-associated ramp protein csm4 (33 kDa, Protein from Streptococcus thermophilus)
- Type iii-a crispr-associated ramp protein csm5 (41 kDa, Protein from Streptococcus thermophilus ND03)
Type III-A Csm complex, Cryo-EM structure of Csm-CTR1, ATP bound
Resolution: 3.37 Å
EM Method: Single-particle
Fitted PDBs: 6ig0
Q-score: 0.461
You L, Ma J, Wang J, Artamonova D, Wang M, Liu L, Xiang H, Severinov K, Zhang X, Wang Y
Cell (2019) 176 pp. 239-253.e16 [ DOI: doi:10.1016/j.cell.2018.10.052 Pubmed: 30503210 ]
- Type iii-a crispr-associated ramp protein csm3 (24 kDa, Protein from Streptococcus thermophilus ND03)
- Ctr1 (13 kDa, RNA from Streptococcus thermophilus ND03)
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Type iii-a crispr-associated protein csm1 (86 kDa, Protein from Streptococcus thermophilus ND03)
- Csm-ctr1 complex, atp bound (290 kDa, Complex from Streptococcus thermophilus ND03)
- Type iii-a crispr-associated ramp protein csm5 (41 kDa, Protein from Streptococcus thermophilus ND03)
- Type iii-a crispr-associated ramp protein csm4 (33 kDa, Protein from Streptococcus thermophilus ND03)
- Crrna (11 kDa, RNA from Streptococcus thermophilus ND03)
- Type iii-a crispr-associated protein csm2 (14 kDa, Protein from Streptococcus thermophilus ND03)
- Zinc ion (65 Da, Ligand)