Structure of CbCas9 bound to 20-nucleotide complementary DNA substrate
Resolution: 2.46 Å
EM Method: Single-particle
Fitted PDBs: 8iyq
Q-score: 0.513
Zhang S, Sun A, Qian JM, Lin S, Xing W, Yang Y, Zhu HZ, Zhou XY, Guo YS, Liu Y, Meng Y, Jin SL, Song W, Li CP, Li Z, Jin S, Wang JH, Dong MQ, Gao C, Chen C, Bai Y, Liu JG
Nature (2024) 630 pp. 484-492 [ Pubmed: 38811729 DOI: doi:10.1038/s41586-024-07486-x ]
- Ts (8 kDa, DNA from Chryseobacterium)
- Nts (8 kDa, DNA from Chryseobacterium)
- Sgrna (40 kDa, RNA from Chryseobacterium)
- Deadcbcas9 (170 kDa, Protein from Chryseobacterium)
- Structure of cbcas9 bound to 20-nucleotide complementary dna substrate (228 kDa, Complex from Chryseobacterium)
Cryo-EM structure of GeoCas9-sgRNA-dsDNA ternary complex
Resolution: 3.08 Å
EM Method: Single-particle
Fitted PDBs: 8jtj
Q-score: 0.421
Shen P, Zhang L, Liu B, Li X, Wang C, Min J, Huang JW, Chen CC, Guo RT
ACS Catal (2024) 14 pp. 13227-13235 [ DOI: doi:10.1021/acscatal.4c03278 ]
- Dna (5'-d(p*gp*gp*gp*cp*gp*cp*gp*ap*a)-3') (2 kDa, DNA from Geobacillus stearothermophilus)
- Type ii crispr rna-guided endonuclease cas9 (130 kDa, Complex from Geobacillus stearothermophilus)
- Dna (29-mer) (8 kDa, DNA from Geobacillus stearothermophilus)
- Rna (139-mer) (44 kDa, RNA from Geobacillus stearothermophilus)
- Crispr-associated endonuclease cas9 (128 kDa, Protein from Geobacillus stearothermophilus)
Cryo-EM structure of GeoCas9-sgRNA binary complex
Resolution: 3.21 Å
EM Method: Single-particle
Fitted PDBs: 8jtr
Q-score: 0.401
Shen P, Zhang L, Liu B, Li X, Wang C, Min J, Huang JW, Chen CC, Guo RT
ACS Catal (2024) 14 pp. 13227-13235 [ DOI: doi:10.1021/acscatal.4c03278 ]
- Crispr-associated endonuclease cas9 (128 kDa, Protein from Geobacillus stearothermophilus)
- Type ii crispr rna-guided endonuclease cas9 (130 kDa, Complex from Geobacillus stearothermophilus)
- Sgrna (139-bp) (44 kDa, RNA from Geobacillus stearothermophilus)
Structure of CbCas9 bound to 6-nucleotide complementary DNA substrate
Resolution: 2.6 Å
EM Method: Single-particle
Fitted PDBs: 8wmh
Q-score: 0.279
Zhang S, Sun A, Qian JM, Lin S, Xing W, Yang Y, Zhu HZ, Zhou XY, Guo YS, Liu Y, Meng Y, Jin SL, Song W, Li CP, Li Z, Jin S, Wang JH, Dong MQ, Gao C, Chen C, Bai Y, Liu JG
Nature (2024) 630 pp. 484-492 [ Pubmed: 38811729 DOI: doi:10.1038/s41586-024-07486-x ]
- Ts (8 kDa, DNA from Chryseobacterium)
- Structure of cbcas9 bound to 6-nucleotide complementary dna substrate (228 kDa, Complex from Chryseobacterium)
- Nts (8 kDa, DNA from Chryseobacterium)
- Sgrna (40 kDa, RNA from Chryseobacterium)
- Deadcbcas9 (170 kDa, Protein from Chryseobacterium)
Structure of CbCas9-PcrIIC1 complex bound to 28-bp DNA substrate (20-nt complementary)
Resolution: 2.98 Å
EM Method: Single-particle
Fitted PDBs: 8wmm
Q-score: 0.342
Zhang S, Sun A, Qian JM, Lin S, Xing W, Yang Y, Zhu HZ, Zhou XY, Guo YS, Liu Y, Meng Y, Jin SL, Song W, Li CP, Li Z, Jin S, Wang JH, Dong MQ, Gao C, Chen C, Bai Y, Liu JG
Nature (2024) 630 pp. 484-492 [ Pubmed: 38811729 DOI: doi:10.1038/s41586-024-07486-x ]
- Magnesium ion (24 Da, Ligand)
- Pcriic1 (15 kDa, Protein from Chryseobacterium)
- Ts (8 kDa, DNA from Chryseobacterium)
- Structure of cbcas9-pcriic1 complex bound to 28-bp dna substrate (20-nt complementary) (469 kDa, Complex from Chryseobacterium)
- Nts (8 kDa, DNA from Chryseobacterium)
- Sgrna (40 kDa, RNA from Chryseobacterium)
- Deadcbcas9 (170 kDa, Protein from Chryseobacterium)
Structure of CbCas9-PcrIIC1 complex bound to 62-bp DNA substrate (symmetric 20-nt complementary)
Resolution: 2.82 Å
EM Method: Single-particle
Fitted PDBs: 8wmn
Q-score: 0.274
Zhang S, Sun A, Qian JM, Lin S, Xing W, Yang Y, Zhu HZ, Zhou XY, Guo YS, Liu Y, Meng Y, Jin SL, Song W, Li CP, Li Z, Jin S, Wang JH, Dong MQ, Gao C, Chen C, Bai Y, Liu JG
Nature (2024) 630 pp. 484-492 [ Pubmed: 38811729 DOI: doi:10.1038/s41586-024-07486-x ]
- Magnesium ion (24 Da, Ligand)
- Sgrna (40 kDa, RNA from Chryseobacterium)
- Structure of cbcas9-pcriic1 complex bound to 62-bp dna substrate (symmetric 20-nt complementary) (471 kDa, Complex from Chryseobacterium)
- Pcriic1 (15 kDa, Protein from Chryseobacterium)
- Dna (62-mer) (19 kDa, DNA from Chryseobacterium)
- Deadcbcas9 (170 kDa, Protein from Chryseobacterium)
Structure of CbCas9-PcrIIC1 complex bound to 62-bp DNA substrate (non-targeting complex)
Resolution: 3.07 Å
EM Method: Single-particle
Fitted PDBs: 8wr4
Q-score: 0.289
Zhang S, Sun A, Qian JM, Lin S, Xing W, Yang Y, Zhu HZ, Zhou XY, Guo YS, Liu Y, Meng Y, Jin SL, Song W, Li CP, Li Z, Jin S, Wang JH, Dong MQ, Gao C, Chen C, Bai Y, Liu JG
Nature (2024) 630 pp. 484-492 [ Pubmed: 38811729 DOI: doi:10.1038/s41586-024-07486-x ]
- Dna (62-mer) (19 kDa, DNA from Chryseobacterium)
- Magnesium ion (24 Da, Ligand)
- Pcriic1 (15 kDa, Protein from Chryseobacterium)
- Dna (62-mer) (18 kDa, DNA from Chryseobacterium)
- Structure of cbcas9-pcriic1 complex bound to 62-bp dna substrate (non-targeting complex) (471 kDa, Complex from Chryseobacterium)
- Sgrna effector-2 (40 kDa, RNA from Chryseobacterium)
- Cbcas9 effector-1 (170 kDa, Protein from Chryseobacterium)
Cryo-EM structure of GeoCas9 in complex with sgRNA and target DNA
Resolution: 3.17 Å
EM Method: Single-particle
Fitted PDBs: 8uza
Q-score: 0.516
Eggers AR, Chen K, Soczek KM, Tuck OT, Doherty EE, Xu B, Trinidad MI, Thornton BW, Yoon PH, Doudna JA
Cell (2024) 187 pp. 3249- [ DOI: doi:10.1016/j.cell.2024.04.031 Pubmed: 38781968 ]
- Non-target strand dna (15 kDa, DNA from Mus musculus)
- Ternary complex of deactivated wild type geocas9 with sgrna and target dna (203 kDa, Complex from Geobacillus stearothermophilus)
- Crispr-associated endonuclease cas9 (127 kDa, Protein from Geobacillus stearothermophilus)
- Target strand dna (15 kDa, DNA from Mus musculus)
- Sgrna (107-mer) (44 kDa, RNA from Geobacillus stearothermophilus)
Cryo-EM structure of iGeoCas9 in complex with sgRNA and target DNA
Resolution: 2.63 Å
EM Method: Single-particle
Fitted PDBs: 8uzb
Q-score: 0.561
Eggers AR, Chen K, Soczek KM, Tuck OT, Doherty EE, Xu B, Trinidad MI, Thornton BW, Yoon PH, Doudna JA
Cell (2024) 187 pp. 3249- [ DOI: doi:10.1016/j.cell.2024.04.031 Pubmed: 38781968 ]
- Non-target strand dna (15 kDa, DNA from Mus musculus)
- Crispr-associated endonuclease cas9 (127 kDa, Protein from Geobacillus stearothermophilus)
- Ternary complex of deactivated igeocas9 with sgrna and target dna (203 kDa, Complex from Geobacillus stearothermophilus)
- Target strand dna (39-mer) (15 kDa, DNA from Mus musculus)
- Rna (107-mer) (44 kDa, RNA from Geobacillus stearothermophilus)
Cryo-EM structure of the EvCas9-sgRNA-target DNA ternary complex
Resolution: 3.43 Å
EM Method: Single-particle
Fitted PDBs: 8hud
Q-score: 0.404
Tang N, Wu Z, Gao Y, Chen W, Wang Z, Su M, Ji W, Ji Q
ACS Synth Biol (2024) 13 pp. 269-281 [ DOI: doi:10.1021/acssynbio.3c00501 Pubmed: 38061052 ]
- Target dna strand (8 kDa, DNA from synthetic construct)
- Sgrna (23 kDa, RNA from synthetic construct)
- Non-target dna strand (2 kDa, DNA from synthetic construct)
- Crispr-associated endonuclease cas9 (131 kDa, Protein from Eubacterium ventriosum ATCC 27560)
- Ternary complex of evcas9-sgrna-dsdna (156 kDa, Complex from Eubacterium ventriosum ATCC 27560)