Cryo-EM structure of GeoCas9-sgRNA-dsDNA ternary complex
Resolution: 3.08 Å
EM Method: Single-particle
Fitted PDBs: 8jtj
Q-score: 0.421
Shen P, Zhang L, Liu B, Li X, Wang C, Min J, Huang JW, Chen CC, Guo RT
ACS Catal (2024) 14 pp. 13227-13235 [ DOI: doi:10.1021/acscatal.4c03278 ]
- Dna (5'-d(p*gp*gp*gp*cp*gp*cp*gp*ap*a)-3') (2 kDa, DNA from Geobacillus stearothermophilus)
- Type ii crispr rna-guided endonuclease cas9 (130 kDa, Complex from Geobacillus stearothermophilus)
- Dna (29-mer) (8 kDa, DNA from Geobacillus stearothermophilus)
- Rna (139-mer) (44 kDa, RNA from Geobacillus stearothermophilus)
- Crispr-associated endonuclease cas9 (128 kDa, Protein from Geobacillus stearothermophilus)
Cryo-EM structure of GeoCas9-sgRNA binary complex
Resolution: 3.21 Å
EM Method: Single-particle
Fitted PDBs: 8jtr
Q-score: 0.401
Shen P, Zhang L, Liu B, Li X, Wang C, Min J, Huang JW, Chen CC, Guo RT
ACS Catal (2024) 14 pp. 13227-13235 [ DOI: doi:10.1021/acscatal.4c03278 ]
- Crispr-associated endonuclease cas9 (128 kDa, Protein from Geobacillus stearothermophilus)
- Type ii crispr rna-guided endonuclease cas9 (130 kDa, Complex from Geobacillus stearothermophilus)
- Sgrna (139-bp) (44 kDa, RNA from Geobacillus stearothermophilus)
Cryo-EM structure of GeoCas9 in complex with sgRNA and target DNA
Resolution: 3.17 Å
EM Method: Single-particle
Fitted PDBs: 8uza
Q-score: 0.516
Eggers AR, Chen K, Soczek KM, Tuck OT, Doherty EE, Xu B, Trinidad MI, Thornton BW, Yoon PH, Doudna JA
Cell (2024) 187 pp. 3249- [ DOI: doi:10.1016/j.cell.2024.04.031 Pubmed: 38781968 ]
- Non-target strand dna (15 kDa, DNA from Mus musculus)
- Ternary complex of deactivated wild type geocas9 with sgrna and target dna (203 kDa, Complex from Geobacillus stearothermophilus)
- Crispr-associated endonuclease cas9 (127 kDa, Protein from Geobacillus stearothermophilus)
- Target strand dna (15 kDa, DNA from Mus musculus)
- Sgrna (107-mer) (44 kDa, RNA from Geobacillus stearothermophilus)
Cryo-EM structure of iGeoCas9 in complex with sgRNA and target DNA
Resolution: 2.63 Å
EM Method: Single-particle
Fitted PDBs: 8uzb
Q-score: 0.561
Eggers AR, Chen K, Soczek KM, Tuck OT, Doherty EE, Xu B, Trinidad MI, Thornton BW, Yoon PH, Doudna JA
Cell (2024) 187 pp. 3249- [ DOI: doi:10.1016/j.cell.2024.04.031 Pubmed: 38781968 ]
- Non-target strand dna (15 kDa, DNA from Mus musculus)
- Crispr-associated endonuclease cas9 (127 kDa, Protein from Geobacillus stearothermophilus)
- Ternary complex of deactivated igeocas9 with sgrna and target dna (203 kDa, Complex from Geobacillus stearothermophilus)
- Target strand dna (39-mer) (15 kDa, DNA from Mus musculus)
- Rna (107-mer) (44 kDa, RNA from Geobacillus stearothermophilus)
CryoEM structure of HpaCas9-sgRNA-dsDNA in the presence of AcrIIC4
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 8hnv
Q-score: 0.464
Sun W, Cheng Z, Wang J, Yang J, Li X, Wang J, Chen M, Yang X, Sheng G, Lou J, Wang Y
PNAS (2023) 120 pp. e2303675120-e2303675120 [ DOI: doi:10.1073/pnas.2303675120 Pubmed: 37494395 ]
- Sgrna (40 kDa, RNA from synthetic construct)
- Rna/dna (Complex)
- Crispr-associated endonuclease cas9 (121 kDa, Protein from Haemophilus parainfluenzae)
- Target strand (10 kDa, DNA from Haemophilus parainfluenzae)
- Non-target strand (10 kDa, DNA from Haemophilus parainfluenzae)
- Complex of hpacas9-sgrna-dna in the presence of acriic4 (194 kDa, Complex from Haemophilus parainfluenzae)
- Anti-crispr protein acriic4 (10 kDa, Protein from Haemophilus parainfluenzae)
- Hpacas9/acriic4 (Complex)
Cryo-EM structure of spyCas9-sgRNA-DNA dimer
Resolution: 5.26 Å
EM Method: Single-particle
Fitted PDBs: 7v59
Q-score: 0.153
Yang M, Sun R, Deng P, Yang Y, Wang W, Liu JG, Chen C
Chem Sci (2021) 12 pp. 12776-12784 [ DOI: doi:10.1039/d1sc02633j Pubmed: 34703564 ]
- Dna (49-mer) (15 kDa, DNA from Streptococcus pyogenes)
- Rna (115-mer) (37 kDa, RNA from Streptococcus pyogenes)
- Ternary complex of spycas9 with sgrna and dna (Complex from Streptococcus pyogenes)
- Crispr-associated endonuclease cas9/csn1 (158 kDa, Protein from Streptococcus pyogenes serotype M1)
Structures of a CRISPR-Cas9 R-loop complex primed for DNA cleavage
Jiang F, Taylor DW, Chen JS, Kornfeld JE, Zhou K, Thompson AJ, Nogales E, Doudna JA
Science (2016) 351 pp. 867-871 [ DOI: doi:10.1126/science.aad8282 Pubmed: 26841432 ]
- Cas9 bound to single guide-rna (194 kDa, Sample)
- Crispr-associated endonuclease cas9/csn1 (158 kDa, Protein from Streptococcus pyogenes)
- Single guide-rna (36 kDa, RNA from Streptococcus pyogenes)
Structures of a CRISPR-Cas9 R-loop complex primed for DNA cleavage
Jiang F, Taylor DW, Chen JS, Kornfeld JE, Zhou K, Thompson AJ, Nogales E, Doudna JA
Science (2016) 351 pp. 867-871 [ DOI: doi:10.1126/science.aad8282 Pubmed: 26841432 ]
- Cas9 bound to single guide-rna and 40-bp target dna (218 kDa, Sample)
- Single guide-rna (36 kDa, RNA from Streptococcus pyogenes)
- Target 40-bp double stranded dna (24 kDa, DNA)
- Crispr-associated endonuclease cas9/csn1 (158 kDa, Protein from Streptococcus pyogenes)