CryoEM structure of ThermoCas9 in post-cleavage state bound with the DNA containing NNNNCCA PAM
Resolution: 2.2 Å
EM Method: Single-particle
Fitted PDBs: 9ar4
Q-score: 0.558
Roth M, Shu Y, Trasanidis D, Zhao Y, Hoffman R, van der Oost J, Li H
To Be Published
- Single guide rna (47 kDa, RNA from Geobacillus thermodenitrificans)
- Cleaved 5' target dna strand (4 kDa, DNA from synthetic construct)
- Cleaved 5' non-target dna strand (1 kDa, DNA from synthetic construct)
- Magnesium ion (24 Da, Ligand)
- Water (18 Da, Ligand)
- Thermocas9 post-cleavage complex (Complex from Geobacillus thermodenitrificans)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Cleaved 3' target dna strand (7 kDa, DNA from synthetic construct)
- Cleaved 3' non-target dna strand (4 kDa, DNA from synthetic construct)
- Crispr-associated endonuclease cas9 (126 kDa, Protein from Geobacillus thermodenitrificans)
Structure of Acidothermus cellulolyticus Cas9 bound with methylated DNA
Resolution: 2.88 Å
EM Method: Single-particle
Fitted PDBs: 9ar5
Q-score: -0.063
Roth MO, Shu Y, Zhao Y, Trasanidou D, Hoffman RD, Trasanidis N, Gelasco MK, Medina ML, Das D, Rai J, Wang B, Li H
To Be Published
- Dna/rna (5'-d(p*tp*ap*cp*a)-r(p*(5mc))-d(p*cp*ap*ap*gp*cp*t)-3') (3 kDa, DNA from Acidothermus cellulolyticus)
- Crispr-associated endonuclease, csn1 family (127 kDa, Protein from Acidothermus cellulolyticus)
- Crispr-cas9 (Complex from Acidothermus cellulolyticus)
- Rna (92-mer) (34 kDa, RNA from Acidothermus cellulolyticus)
- Dna (25-mer) (7 kDa, DNA from Acidothermus cellulolyticus)
CryoEM structure of ThermoCas9 in pre-cleavage state bound with the DNA containing NNNNCCA PAM
Resolution: 2.77 Å
EM Method: Single-particle
Fitted PDBs: 9ar6
Q-score: 0.501
Roth M, Shu Y, Trasanidou D, Zhao Y, van der Oost J, Li H
To Be Published
- Ternary complex of thermocas9 with single guide rna and target dna (Complex from Geobacillus thermodenitrificans)
- Water (18 Da, Ligand)
- Sodium ion (22 Da, Ligand)
- Target-strand dna (12 kDa, DNA from Geobacillus thermodenitrificans)
- Non-target dna (3 kDa, DNA from Geobacillus thermodenitrificans)
- Crispr-associated endonuclease cas9 (126 kDa, Protein from Geobacillus thermodenitrificans)
- Single guide rna (47 kDa, RNA from Geobacillus thermodenitrificans)
CryoEM structure of ThermoCas9 bound with target DNA strand only
Resolution: 2.52 Å
EM Method: Single-particle
Fitted PDBs: 9ar7
Q-score: 0.518
Roth M, Shu Y, Trasandidou D, Zhao Y, van der Oost J, Li H
To Be Published
- Magnesium ion (24 Da, Ligand)
- Water (18 Da, Ligand)
- Cleaved 5' target strand (4 kDa, DNA from Geobacillus thermodenitrificans)
- Cleaved 3' target dna strand (12 kDa, DNA from Geobacillus thermodenitrificans)
- Single guide rna (47 kDa, RNA from Geobacillus thermodenitrificans)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Ternary complex of thermocas9 with rna and dna (Complex from Geobacillus thermodenitrificans NG80-2)
- Crispr-associated endonuclease cas9 (126 kDa, Protein from Geobacillus thermodenitrificans)
Cryo-EM structure of SaCas9-sgRNA-DNA ternary complex
Du W, Zhu H, Qian J, Xue D, Zheng S, Huang Q
Int J Mol Sci (2023) 24 [ Pubmed: 36674715 DOI: doi:10.3390/ijms24021204 ]
- Single-guide rna (sgrna) (RNA from synthetic construct)
- Target dna strand(ts) (DNA from synthetic construct)
- Sacas9-sgrna-target dna ternary complex (Complex from Staphylococcus aureus)
- Sgrna-target dna (Complex)
- Non-target dna strand (nts) (DNA from synthetic construct)
- Sacas9 (Protein from Staphylococcus aureus)
- Sacas9 (Complex)
Cryo-EM structure of St1Cas9-sgRNA-tDNA20-AcrIIA6 monomeric assembly.
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 6rj9
Q-score: 0.542
Fuchsbauer O, Swuec P, Zimberger C, Amigues B, Levesque S, Agudelo D, Duringer A, Chaves-Sanjuan A, Spinelli S, Rousseau GM, Velimirovic M, Bolognesi M, Roussel A, Cambillau C, Moineau S, Doyon Y, Goulet A
Mol Cell (2019) 76 pp. 922- [ DOI: doi:10.1016/j.molcel.2019.09.012 Pubmed: 31604602 ]
- Crispr-associated endonuclease cas9 1 (129 kDa, Protein from Streptococcus thermophilus (strain ATCC BAA-491 / LMD-9))
- Ntpam (Complex from Streptococcus virus 2972)
- Crispr-associated endonuclease cas9 (Complex from Streptococcus thermophilus (strain ATCC BAA-491 / LMD-9))
- Acriia6 (21 kDa, Protein from Streptococcus phage D1811)
- Cryo-em structure of st1cas9-sgrna-tdna20-acriia6 monomeric assembly. (Complex)
- Sgrna (37 kDa, RNA from Streptococcus thermophilus)
- Acriia6 (Complex from Streptococcus phage D1811)
- Tdna20 (6 kDa, DNA from Streptococcus virus 2972)
- Sgrna (Complex from Streptococcus thermophilus)
Cryo-EM structure of St1Cas9-sgRNA-tDNA20-AcrIIA6 dimeric assembly.
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 6rja
Q-score: 0.508
Fuchsbauer O, Swuec P, Zimberger C, Amigues B, Levesque S, Agudelo D, Duringer A, Chaves-Sanjuan A, Spinelli S, Rousseau GM, Velimirovic M, Bolognesi M, Roussel A, Cambillau C, Moineau S, Doyon Y, Goulet A
Mol Cell (2019) 76 pp. 922- [ DOI: doi:10.1016/j.molcel.2019.09.012 Pubmed: 31604602 ]
- Crispr-associated endonuclease cas9 1 (129 kDa, Protein from Streptococcus thermophilus (strain ATCC BAA-491 / LMD-9))
- Crispr-associated endonuclease cas9 (Complex from Streptococcus thermophilus (strain ATCC BAA-491 / LMD-9))
- Acriia6 (21 kDa, Protein from Streptococcus phage D1811)
- Cryo-em structure of st1cas9-sgrna-tdna20-acriia6 dimeric assembly. (Complex)
- Tdna20 (Complex from Streptococcus virus 2972)
- Acriia6 (Complex from Streptococcus phage D1811)
- Rna (78-mer) (Complex from Streptococcus thermophilus)
- Rna (78-mer) (37 kDa, RNA from Streptococcus thermophilus)
- Tdna20 (6 kDa, DNA from Streptococcus virus 2972)
Cryo-EM structure of St1Cas9-sgRNA-tDNA59-ntPAM complex.
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 6rjd
Q-score: 0.5
Fuchsbauer O, Swuec P, Zimberger C, Amigues B, Levesque S, Agudelo D, Duringer A, Chaves-Sanjuan A, Spinelli S, Rousseau GM, Velimirovic M, Bolognesi M, Roussel A, Cambillau C, Moineau S, Doyon Y, Goulet A
Mol Cell (2019) 76 pp. 922- [ DOI: doi:10.1016/j.molcel.2019.09.012 Pubmed: 31604602 ]
- Ntpam (Complex from Streptococcus phage 2972)
- Tdna59 (18 kDa, DNA from Streptococcus phage D1811)
- Cryo-em structure of st1cas9-sgrna-tdna59-ntpam complex. (Complex)
- Sgrna (78-mer) (Complex from Streptococcus thermophilus)
- Ntpam (7 kDa, DNA from Streptococcus Phage 2972)
- Streptococcus thermophilus 1 cas9 (129 kDa, Protein from Streptococcus thermophilus DGCC 7710)
- Sgrna (78-mer) (37 kDa, RNA from Streptococcus thermophilus DGCC 7710)
- Streptococcus thermophilus 1 cas9 (Complex from Streptococcus thermophilus DGCC 7710)
- Tdna59 (Complex from Streptococcus phage D1811)
Cryo-EM structure of St1Cas9-sgRNA-AcrIIA6-tDNA59-ntPAM complex.
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 6rjg
Q-score: 0.522
Fuchsbauer O, Swuec P, Zimberger C, Amigues B, Levesque S, Agudelo D, Duringer A, Chaves-Sanjuan A, Spinelli S, Rousseau GM, Velimirovic M, Bolognesi M, Roussel A, Cambillau C, Moineau S, Doyon Y, Goulet A
Mol Cell (2019) 76 pp. 922- [ DOI: doi:10.1016/j.molcel.2019.09.012 Pubmed: 31604602 ]
- Ntpam (Complex from Streptococcus virus 2972)
- Acriia6 (21 kDa, Protein from Streptococcus phage D1811)
- Ntpam (7 kDa, DNA from Streptococcus virus 2972)
- Cas 9 (129 kDa, Protein from Streptococcus thermophilus DGCC 7710)
- Cas 9 (Complex from Streptococcus thermophilus DGCC 7710)
- Tdna59 (18 kDa, DNA from Streptococcus virus 2972)
- Tdna59 (Complex from Streptococcus virus 2972)
- Sgrna (37 kDa, RNA from Streptococcus thermophilus)
- Acriia6 (Complex from Streptococcus phage D1811)
- Cryo-em structure of st1cas9-sgrna-acriia6-tdna59-ntpam complex. (Complex)
- Sgrna (Complex from Streptococcus thermophilus)
Cryo-EM structure of SpCas9-sgRNA-DNA ternary complex
Resolution: 5.2 Å
EM Method: Single-particle
Fitted PDBs: 5y36
Q-score: 0.093