Cryo-EM structure of GeoCas9 in complex with sgRNA and target DNA
Resolution: 3.17 Å
EM Method: Single-particle
Fitted PDBs: 8uza
Q-score: 0.516
Eggers AR, Chen K, Soczek KM, Tuck OT, Doherty EE, Xu B, Trinidad MI, Thornton BW, Yoon PH, Doudna JA
Cell (2024) 187 pp. 3249- [ DOI: doi:10.1016/j.cell.2024.04.031 Pubmed: 38781968 ]
- Non-target strand dna (15 kDa, DNA from Mus musculus)
- Ternary complex of deactivated wild type geocas9 with sgrna and target dna (203 kDa, Complex from Geobacillus stearothermophilus)
- Crispr-associated endonuclease cas9 (127 kDa, Protein from Geobacillus stearothermophilus)
- Target strand dna (15 kDa, DNA from Mus musculus)
- Sgrna (107-mer) (44 kDa, RNA from Geobacillus stearothermophilus)
Cryo-EM structure of iGeoCas9 in complex with sgRNA and target DNA
Resolution: 2.63 Å
EM Method: Single-particle
Fitted PDBs: 8uzb
Q-score: 0.561
Eggers AR, Chen K, Soczek KM, Tuck OT, Doherty EE, Xu B, Trinidad MI, Thornton BW, Yoon PH, Doudna JA
Cell (2024) 187 pp. 3249- [ DOI: doi:10.1016/j.cell.2024.04.031 Pubmed: 38781968 ]
- Non-target strand dna (15 kDa, DNA from Mus musculus)
- Crispr-associated endonuclease cas9 (127 kDa, Protein from Geobacillus stearothermophilus)
- Ternary complex of deactivated igeocas9 with sgrna and target dna (203 kDa, Complex from Geobacillus stearothermophilus)
- Target strand dna (39-mer) (15 kDa, DNA from Mus musculus)
- Rna (107-mer) (44 kDa, RNA from Geobacillus stearothermophilus)
Cas9 with double-stranded guide RNA and target DNA
Resolution: 3.03 Å
EM Method: Single-particle
Fitted PDBs: 8fzt
Q-score: 0.427
To Be Published
- E2_ts (12 kDa, DNA from Homo sapiens)
- Tracrrna (22 kDa, RNA from Streptococcus pyogenes)
- Crispr-associated endonuclease cas9/csn1 (160 kDa, Protein from Streptococcus pyogenes)
- E2-nts (12 kDa, DNA from Homo sapiens)
- Crispr-associated endonuclease cas9 complex with double stranded guide rna and target dna (Complex from Streptococcus pyogenes)
- Cre2 (13 kDa, RNA from Streptococcus pyogenes)
SpCas9 with sgRNA and target DNA
Resolution: 3.12 Å
EM Method: Single-particle
Fitted PDBs: 8g1i
Q-score: 0.392
To Be Published
- Crispr-associated endonuclease cas9/csn1 (160 kDa, Protein from Streptococcus pyogenes)
- Crispr-associated endonuclease cas9 complex with sgrna and target dna (Complex from Streptococcus pyogenes)
- Dna e2_nts (12 kDa, DNA from Renilla reniformis)
- Sgrna (31 kDa, RNA from Streptococcus pyogenes)
- Dna e2_ts (12 kDa, DNA from Renilla reniformis)
Cryo-EM structure of Cas9 in complex with 12-14MM DNA substrate, 5 minute time-point
Resolution: 3.56 Å
EM Method: Single-particle
Fitted PDBs: 7s4u
Q-score: 0.419
Bravo JPK, Liu MS, Hibshman GN, Dangerfield TL, Jung K, McCool RS, Johnson KA, Taylor DW
Nature (2022) 603 pp. 343-347 [ DOI: doi:10.1038/s41586-022-04470-1 Pubmed: 35236982 ]
- Target strand (10 kDa, DNA from synthetic construct)
- Sars-cov-2 rdrp complex with template:primer and four rmp (Complex from Streptococcus pyogenes)
- Crispr-associated endonuclease cas9/csn1 (158 kDa, Protein from Streptococcus pyogenes)
- Grna (31 kDa, RNA from synthetic construct)
- Non-target strand (5 kDa, DNA from synthetic construct)
Cas9 bound to 12-14MM DNA, 60 min time-point, kinked conformation
Resolution: 3.28 Å
EM Method: Single-particle
Fitted PDBs: 7s4v
Q-score: 0.448
Bravo JPK, Liu MS, Hibshman GN, Dangerfield TL, Jung K, McCool RS, Johnson KA, Taylor DW
Nature (2022) 603 pp. 343-347 [ DOI: doi:10.1038/s41586-022-04470-1 Pubmed: 35236982 ]
- Ts (10 kDa, DNA from synthetic construct)
- Crispr-associated endonuclease cas9/csn1 (158 kDa, Protein from Streptococcus pyogenes)
- Nts (9 kDa, DNA from synthetic construct)
- Cas9 bound to 12-14mm dna, 60 min time-point, kinked conformation (Complex from Streptococcus pyogenes)
- Grna (31 kDa, RNA from synthetic construct)
Cas9 in complex with 15-17MM DNA, 60 min time-point
Bravo JPK, Liu MS, Hibshman GN, Dangerfield TL, Jung K, McCool RS, Johnson KA, Taylor DW
Nature (2022) 603 pp. 343-347 [ DOI: doi:10.1038/s41586-022-04470-1 Pubmed: 35236982 ]
Cas9 in complex with 18-20MM DNA, 1 min time-point, linear conformation
Bravo JPK, Liu MS, Hibshman GN, Dangerfield TL, Jung K, McCool RS, Johnson KA, Taylor DW
Nature (2022) 603 pp. 343-347 [ DOI: doi:10.1038/s41586-022-04470-1 Pubmed: 35236982 ]
Cas9:gRNA in complex with 18-20MM DNA, 1 minute time-point, kinked active conformation
Resolution: 2.76 Å
EM Method: Single-particle
Fitted PDBs: 7s4x
Q-score: 0.551
Bravo JPK, Liu MS, Hibshman GN, Dangerfield TL, Jung K, McCool RS, Johnson KA, Taylor DW
Nature (2022) 603 pp. 343-347 [ DOI: doi:10.1038/s41586-022-04470-1 Pubmed: 35236982 ]
- Magnesium ion (24 Da, Ligand)
- Water (18 Da, Ligand)
- Nts (10 kDa, DNA from synthetic construct)
- Ts (4 kDa, DNA from synthetic construct)
- Crispr-associated endonuclease cas9/csn1 (158 kDa, Protein from Streptococcus pyogenes)
- Cas9 bound to 18-20mm dna, 1 min time-point, kinked active conformation (Complex from Streptococcus pyogenes)
- Grna (31 kDa, RNA from synthetic construct)
- Ts (6 kDa, DNA from synthetic construct)
Structures of a CRISPR-Cas9 R-loop complex primed for DNA cleavage
Jiang F, Taylor DW, Chen JS, Kornfeld JE, Zhou K, Thompson AJ, Nogales E, Doudna JA
Science (2016) 351 pp. 867-871 [ DOI: doi:10.1126/science.aad8282 Pubmed: 26841432 ]
- Cas9 bound to single guide-rna (194 kDa, Sample)
- Crispr-associated endonuclease cas9/csn1 (158 kDa, Protein from Streptococcus pyogenes)
- Single guide-rna (36 kDa, RNA from Streptococcus pyogenes)