Structure of Acidothermus cellulolyticus Cas9 bound with methylated DNA
Resolution: 2.88 Å
EM Method: Single-particle
Fitted PDBs: 9ar5
Q-score: -0.063
Roth MO, Shu Y, Zhao Y, Trasanidou D, Hoffman RD, Trasanidis N, Gelasco MK, Medina ML, Das D, Rai J, Wang B, Li H
To Be Published
- Dna/rna (5'-d(p*tp*ap*cp*a)-r(p*(5mc))-d(p*cp*ap*ap*gp*cp*t)-3') (3 kDa, DNA from Acidothermus cellulolyticus)
- Crispr-associated endonuclease, csn1 family (127 kDa, Protein from Acidothermus cellulolyticus)
- Crispr-cas9 (Complex from Acidothermus cellulolyticus)
- Rna (92-mer) (34 kDa, RNA from Acidothermus cellulolyticus)
- Dna (25-mer) (7 kDa, DNA from Acidothermus cellulolyticus)
Structure of CbCas9 bound to 20-nucleotide complementary DNA substrate
Resolution: 2.46 Å
EM Method: Single-particle
Fitted PDBs: 8iyq
Q-score: 0.513
Zhang S, Sun A, Qian JM, Lin S, Xing W, Yang Y, Zhu HZ, Zhou XY, Guo YS, Liu Y, Meng Y, Jin SL, Song W, Li CP, Li Z, Jin S, Wang JH, Dong MQ, Gao C, Chen C, Bai Y, Liu JG
Nature (2024) 630 pp. 484-492 [ Pubmed: 38811729 DOI: doi:10.1038/s41586-024-07486-x ]
- Ts (8 kDa, DNA from Chryseobacterium)
- Nts (8 kDa, DNA from Chryseobacterium)
- Sgrna (40 kDa, RNA from Chryseobacterium)
- Deadcbcas9 (170 kDa, Protein from Chryseobacterium)
- Structure of cbcas9 bound to 20-nucleotide complementary dna substrate (228 kDa, Complex from Chryseobacterium)
Cryo-EM structure of GeoCas9-sgRNA-dsDNA ternary complex
Resolution: 3.08 Å
EM Method: Single-particle
Fitted PDBs: 8jtj
Q-score: 0.421
Shen P, Zhang L, Liu B, Li X, Wang C, Min J, Huang JW, Chen CC, Guo RT
ACS Catal (2024) 14 pp. 13227-13235 [ DOI: doi:10.1021/acscatal.4c03278 ]
- Dna (5'-d(p*gp*gp*gp*cp*gp*cp*gp*ap*a)-3') (2 kDa, DNA from Geobacillus stearothermophilus)
- Type ii crispr rna-guided endonuclease cas9 (130 kDa, Complex from Geobacillus stearothermophilus)
- Dna (29-mer) (8 kDa, DNA from Geobacillus stearothermophilus)
- Rna (139-mer) (44 kDa, RNA from Geobacillus stearothermophilus)
- Crispr-associated endonuclease cas9 (128 kDa, Protein from Geobacillus stearothermophilus)
Cryo-EM structure of GeoCas9-sgRNA binary complex
Resolution: 3.21 Å
EM Method: Single-particle
Fitted PDBs: 8jtr
Q-score: 0.401
Shen P, Zhang L, Liu B, Li X, Wang C, Min J, Huang JW, Chen CC, Guo RT
ACS Catal (2024) 14 pp. 13227-13235 [ DOI: doi:10.1021/acscatal.4c03278 ]
- Crispr-associated endonuclease cas9 (128 kDa, Protein from Geobacillus stearothermophilus)
- Type ii crispr rna-guided endonuclease cas9 (130 kDa, Complex from Geobacillus stearothermophilus)
- Sgrna (139-bp) (44 kDa, RNA from Geobacillus stearothermophilus)
Structure of CbCas9 bound to 6-nucleotide complementary DNA substrate
Resolution: 2.6 Å
EM Method: Single-particle
Fitted PDBs: 8wmh
Q-score: 0.279
Zhang S, Sun A, Qian JM, Lin S, Xing W, Yang Y, Zhu HZ, Zhou XY, Guo YS, Liu Y, Meng Y, Jin SL, Song W, Li CP, Li Z, Jin S, Wang JH, Dong MQ, Gao C, Chen C, Bai Y, Liu JG
Nature (2024) 630 pp. 484-492 [ Pubmed: 38811729 DOI: doi:10.1038/s41586-024-07486-x ]
- Ts (8 kDa, DNA from Chryseobacterium)
- Structure of cbcas9 bound to 6-nucleotide complementary dna substrate (228 kDa, Complex from Chryseobacterium)
- Nts (8 kDa, DNA from Chryseobacterium)
- Sgrna (40 kDa, RNA from Chryseobacterium)
- Deadcbcas9 (170 kDa, Protein from Chryseobacterium)
Structure of CbCas9-PcrIIC1 complex bound to 28-bp DNA substrate (20-nt complementary)
Resolution: 2.98 Å
EM Method: Single-particle
Fitted PDBs: 8wmm
Q-score: 0.342
Zhang S, Sun A, Qian JM, Lin S, Xing W, Yang Y, Zhu HZ, Zhou XY, Guo YS, Liu Y, Meng Y, Jin SL, Song W, Li CP, Li Z, Jin S, Wang JH, Dong MQ, Gao C, Chen C, Bai Y, Liu JG
Nature (2024) 630 pp. 484-492 [ Pubmed: 38811729 DOI: doi:10.1038/s41586-024-07486-x ]
- Magnesium ion (24 Da, Ligand)
- Pcriic1 (15 kDa, Protein from Chryseobacterium)
- Ts (8 kDa, DNA from Chryseobacterium)
- Structure of cbcas9-pcriic1 complex bound to 28-bp dna substrate (20-nt complementary) (469 kDa, Complex from Chryseobacterium)
- Nts (8 kDa, DNA from Chryseobacterium)
- Sgrna (40 kDa, RNA from Chryseobacterium)
- Deadcbcas9 (170 kDa, Protein from Chryseobacterium)
Structure of CbCas9-PcrIIC1 complex bound to 62-bp DNA substrate (symmetric 20-nt complementary)
Resolution: 2.82 Å
EM Method: Single-particle
Fitted PDBs: 8wmn
Q-score: 0.274
Zhang S, Sun A, Qian JM, Lin S, Xing W, Yang Y, Zhu HZ, Zhou XY, Guo YS, Liu Y, Meng Y, Jin SL, Song W, Li CP, Li Z, Jin S, Wang JH, Dong MQ, Gao C, Chen C, Bai Y, Liu JG
Nature (2024) 630 pp. 484-492 [ Pubmed: 38811729 DOI: doi:10.1038/s41586-024-07486-x ]
- Magnesium ion (24 Da, Ligand)
- Sgrna (40 kDa, RNA from Chryseobacterium)
- Structure of cbcas9-pcriic1 complex bound to 62-bp dna substrate (symmetric 20-nt complementary) (471 kDa, Complex from Chryseobacterium)
- Pcriic1 (15 kDa, Protein from Chryseobacterium)
- Dna (62-mer) (19 kDa, DNA from Chryseobacterium)
- Deadcbcas9 (170 kDa, Protein from Chryseobacterium)
Structure of CbCas9-PcrIIC1 complex bound to 62-bp DNA substrate (non-targeting complex)
Resolution: 3.07 Å
EM Method: Single-particle
Fitted PDBs: 8wr4
Q-score: 0.289
Zhang S, Sun A, Qian JM, Lin S, Xing W, Yang Y, Zhu HZ, Zhou XY, Guo YS, Liu Y, Meng Y, Jin SL, Song W, Li CP, Li Z, Jin S, Wang JH, Dong MQ, Gao C, Chen C, Bai Y, Liu JG
Nature (2024) 630 pp. 484-492 [ Pubmed: 38811729 DOI: doi:10.1038/s41586-024-07486-x ]
- Dna (62-mer) (19 kDa, DNA from Chryseobacterium)
- Magnesium ion (24 Da, Ligand)
- Pcriic1 (15 kDa, Protein from Chryseobacterium)
- Dna (62-mer) (18 kDa, DNA from Chryseobacterium)
- Structure of cbcas9-pcriic1 complex bound to 62-bp dna substrate (non-targeting complex) (471 kDa, Complex from Chryseobacterium)
- Sgrna effector-2 (40 kDa, RNA from Chryseobacterium)
- Cbcas9 effector-1 (170 kDa, Protein from Chryseobacterium)
Cryo-EM structure of SaCas9-AcrIIA15 CTD-sgRNA complex
Resolution: 3.31 Å
EM Method: Single-particle
Fitted PDBs: 8jft
Q-score: 0.426
Deng X, Sun W, Li X, Wang J, Cheng Z, Sheng G, Wang Y
Nat Commun (2024) 15 pp. 1806-1806 [ DOI: doi:10.1038/s41467-024-45987-5 Pubmed: 38418450 ]
- Acriia15 (Complex from Staphylococcus delphini)
- Sacas9 (Complex from Staphylococcus aureus)
- Sgrna (Complex from Staphylococcus aureus)
- Crispr-associated endonuclease cas9 (124 kDa, Protein from Staphylococcus aureus)
- Sgrna of sacas9 (31 kDa, RNA from Staphylococcus aureus)
- Acriia15 (13 kDa, Protein from Staphylococcus delphini)
- Ternary complex of sacas9-acriia15 ctd-sgrna complex (Complex)
Cryo-EM structure of the SaCas9-sgRNA-AcrIIA15-promoter DNA dimer
Resolution: 3.82 Å
EM Method: Single-particle
Fitted PDBs: 8jg9
Q-score: 0.173
Deng X, Sun W, Li X, Wang J, Cheng Z, Sheng G, Wang Y
Nat Commun (2024) 15 pp. 1806-1806 [ DOI: doi:10.1038/s41467-024-45987-5 Pubmed: 38418450 ]
- Acriia15 (20 kDa, Protein from Staphylococcus delphini)
- Sgrna (31 kDa, RNA from Staphylococcus aureus)
- Acriia15 (Complex)
- Crispr-associated endonuclease cas9 (124 kDa, Protein from Staphylococcus aureus)
- Sacas9-sgrna-acriia15-promoter dna complex (Complex from Staphylococcus aureus)
- Dna (25-mer) (7 kDa, DNA from Staphylococcus delphini)
- Dna (Complex)
- Sgrna (Complex from Staphylococcus delphini)
- Dna (25-mer) (7 kDa, DNA from Staphylococcus delphini)
- Sacas9 (Complex)