Cryo-EM structure of GeoCas9 in complex with sgRNA and target DNA
Resolution: 3.17 Å
EM Method: Single-particle
Fitted PDBs: 8uza
Q-score: 0.516
Eggers AR, Chen K, Soczek KM, Tuck OT, Doherty EE, Xu B, Trinidad MI, Thornton BW, Yoon PH, Doudna JA
Cell (2024) 187 pp. 3249- [ DOI: doi:10.1016/j.cell.2024.04.031 Pubmed: 38781968 ]
- Non-target strand dna (15 kDa, DNA from Mus musculus)
- Ternary complex of deactivated wild type geocas9 with sgrna and target dna (203 kDa, Complex from Geobacillus stearothermophilus)
- Crispr-associated endonuclease cas9 (127 kDa, Protein from Geobacillus stearothermophilus)
- Target strand dna (15 kDa, DNA from Mus musculus)
- Sgrna (107-mer) (44 kDa, RNA from Geobacillus stearothermophilus)
Cryo-EM structure of iGeoCas9 in complex with sgRNA and target DNA
Resolution: 2.63 Å
EM Method: Single-particle
Fitted PDBs: 8uzb
Q-score: 0.561
Eggers AR, Chen K, Soczek KM, Tuck OT, Doherty EE, Xu B, Trinidad MI, Thornton BW, Yoon PH, Doudna JA
Cell (2024) 187 pp. 3249- [ DOI: doi:10.1016/j.cell.2024.04.031 Pubmed: 38781968 ]
- Non-target strand dna (15 kDa, DNA from Mus musculus)
- Crispr-associated endonuclease cas9 (127 kDa, Protein from Geobacillus stearothermophilus)
- Ternary complex of deactivated igeocas9 with sgrna and target dna (203 kDa, Complex from Geobacillus stearothermophilus)
- Target strand dna (39-mer) (15 kDa, DNA from Mus musculus)
- Rna (107-mer) (44 kDa, RNA from Geobacillus stearothermophilus)
Cryo-EM structure of a riboendonclease
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 8ewg
Q-score: 0.518
Cryo-EM structure of a riboendonuclease
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 8h4u
Q-score: 0.439
CryoEM structure of AcrIIA2 in complex with CRISPR-Cas9
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 6mcb
Q-score: 0.438
Jiang F, Liu JJ, Osuna BA, Xu M, Berry JD, Rauch BJ, Nogales E, Bondy-Denomy J, Doudna JA
Mol Cell (2019) 73 pp. 601- [ Pubmed: 30595438 DOI: doi:10.1016/j.molcel.2018.11.016 ]
- Crispr-associated endonuclease cas9 (158 kDa, Protein from Streptococcus pyogenes M1 GAS)
- Anti-crispr protein acriia2 (14 kDa, Protein from Listeria phage LP-101)
- Acriia2 (Complex from Listeria phage LP-101)
- Single guide rna (116-mer) (37 kDa, RNA from Streptococcus pyogenes M1 GAS)
- Acriia2 interacting with sgrna-bound streptococcus pyogenes cas9 (Complex)
- Sgrna-bound streptococcus pyogenes cas9 (Complex from Streptococcus pyogenes M1 GAS)
CryoEM structure of AcrIIA2 homolog in complex with CRISPR-Cas9
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 6mcc
Q-score: 0.385
Jiang F, Liu JJ, Osuna BA, Xu M, Berry JD, Rauch BJ, Nogales E, Bondy-Denomy J, Doudna JA
Mol Cell (2019) 73 pp. 601- [ Pubmed: 30595438 DOI: doi:10.1016/j.molcel.2018.11.016 ]
- Crispr-associated endonuclease cas9 (158 kDa, Protein from Streptococcus pyogenes M1 GAS)
- Acriia2 homolog (acriia2b) (Complex from Listeria phage LP-101)
- Single guide rna (116-mer) (37 kDa, RNA from Streptococcus pyogenes M1 GAS)
- Acriia2 homolog (acriia2b) bound to sgrna-bound streptococcus pyogenes cas9 (Complex)
- Anti-crispr acriia2 homolog (15 kDa, Protein from Listeria phage LP-101)
- Sgrna-bound streptococcus pyogenes cas9 (Complex from Streptococcus pyogenes M1 GAS)