Structure of the SpCas9 DNA adenine base editor - ABE8e
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 6vpc
Q-score: 0.37
Lapinaite A, Knott GJ, Palumbo CM, Lin-Shiao E, Richter MF, Zhao KT, Beal PA, Liu DR, Doudna JA
Science (2020) 369 pp. 566-571 [ DOI: doi:10.1126/science.abb1390 Pubmed: 32732424 ]
- Cas9 (spcas9) single-guide rna (sgrna) (26 kDa, RNA from Streptococcus pyogenes)
- Crispr-associated endonuclease cas9 (157 kDa, Protein from Streptococcus pyogenes)
- Dna target strand (ts) (15 kDa, DNA from synthetic construct)
- Magnesium ion (24 Da, Ligand)
- Dna non-target strand (nts) (15 kDa, DNA from synthetic construct)
- Zinc ion (65 Da, Ligand)
- T-rna adenine deaminase a v8e (tada-8e) (24 kDa, Protein from Escherichia coli)
- Crispr-cas9 abe8e in a substrate-bound state (Complex from Streptococcus pyogenes)
Structures of a CRISPR-Cas9 R-loop complex primed for DNA cleavage
Jiang F, Taylor DW, Chen JS, Kornfeld JE, Zhou K, Thompson AJ, Nogales E, Doudna JA
Science (2016) 351 pp. 867-871 [ DOI: doi:10.1126/science.aad8282 Pubmed: 26841432 ]
- Cas9 bound to single guide-rna (194 kDa, Sample)
- Crispr-associated endonuclease cas9/csn1 (158 kDa, Protein from Streptococcus pyogenes)
- Single guide-rna (36 kDa, RNA from Streptococcus pyogenes)
Structures of a CRISPR-Cas9 R-loop complex primed for DNA cleavage
Jiang F, Taylor DW, Chen JS, Kornfeld JE, Zhou K, Thompson AJ, Nogales E, Doudna JA
Science (2016) 351 pp. 867-871 [ DOI: doi:10.1126/science.aad8282 Pubmed: 26841432 ]
- Cas9 bound to single guide-rna and 40-bp target dna (218 kDa, Sample)
- Single guide-rna (36 kDa, RNA from Streptococcus pyogenes)
- Target 40-bp double stranded dna (24 kDa, DNA)
- Crispr-associated endonuclease cas9/csn1 (158 kDa, Protein from Streptococcus pyogenes)
Structures of Cas9 endonucleases reveal RNA-mediated conformational activation
Jinek M, Jiang F, Taylor DW, Sternberg SH, Kaya E, Ma E, Anders C, Hauer M, Zhou K, Lin S, Kaplan M, Iavarone AT, Charpentier E, Nogales E, Doudna JA
Science (2014) 343 pp. 1247997- [ Pubmed: 24505130 DOI: doi:10.1126/science.1247997 ]
- Apo-cas9 (160 kDa, Sample)
- Crispr-associated endonuclease cas9 (159 kDa, Protein from Streptococcus pyogenes)
Structures of Cas9 endonucleases reveal RNA-mediated conformational activation
Jinek M, Jiang F, Taylor DW, Sternberg SH, Kaya E, Ma E, Anders C, Hauer M, Zhou K, Lin S, Kaplan M, Iavarone AT, Charpentier E, Nogales E, Doudna JA
Science (2014) 343 pp. 1247997- [ Pubmed: 24505130 DOI: doi:10.1126/science.1247997 ]
- Crispr rna (14 kDa, RNA from Streptococcus pyogenes)
- Crispr-associated endonuclease cas9 (159 kDa, Protein from Streptococcus pyogenes)
- Trans-activating crispr rna (24 kDa, RNA from Streptococcus pyogenes)
- Cas9 loaded with crrna:tracrrna (196 kDa, Sample)
Structures of Cas9 endonucleases reveal RNA-mediated conformational activation
Jinek M, Jiang F, Taylor DW, Sternberg SH, Kaya E, Ma E, Anders C, Hauer M, Zhou K, Lin S, Kaplan M, Iavarone AT, Charpentier E, Nogales E, Doudna JA
Science (2014) 343 pp. 1247997- [ Pubmed: 24505130 DOI: doi:10.1126/science.1247997 ]
- Crispr-associated endonuclease cas9 (159 kDa, Protein from Streptococcus pyogenes)
- 55 bp dna duplex non-target strand (17 kDa, DNA from Enterobacteria phage lambda)
- 55 bp dna duplex target strand (17 kDa, DNA from Enterobacteria phage lambda)
- Crispr rna (14 kDa, RNA from Streptococcus pyogenes)
- Trans-activating crispr rna (24 kDa, RNA from Streptococcus pyogenes)
- Cas9 loaded with crrna:tracrrna and bound to target dna duplex (230 kDa, Sample)