type I-B Cascade bound to a PAM-containing dsDNA target at 3.8 angstrom resolution.
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 8h67
Q-score: 0.307
Lu M, Yu C, Zhang Y, Ju W, Ye Z, Hua C, Mao J, Hu C, Yang Z, Xiao Y
Nat Commun (2024) 15 pp. 4126-4126 [ DOI: doi:10.1038/s41467-024-48598-2 Pubmed: 38750051 ]
- Crispr associated protein cas11b (14 kDa, Protein from Synechocystis sp. PCC 6714)
- Crispr associated protein cas8 (69 kDa, Protein from Synechocystis sp. PCC 6714)
- Cryo-em structure of synechocystis sp. pcc6714 cascade bound to a pam-containing dsdna target at 3.8 angstrom resolution. (Complex from Synechocystis sp. PCC 6714)
- Non target dna (2 kDa, DNA from synthetic construct)
- Crispr associated protein cas5 (26 kDa, Protein from Synechocystis sp. PCC 6714)
- Crispr rna (22 kDa, RNA from synthetic construct)
- Target dna (3 kDa, DNA from synthetic construct)
- Crispr associated protein cas7 (33 kDa, Protein from Synechocystis sp. PCC 6714)
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 8ip0
Q-score: 0.326
Lu M, Yu C, Zhang Y, Ju W, Ye Z, Hua C, Mao J, Hu C, Yang Z, Xiao Y
Nat Commun (2024) 15 pp. 4126-4126 [ DOI: doi:10.1038/s41467-024-48598-2 Pubmed: 38750051 ]
- Dna (41-mer) (12 kDa, DNA from synthetic construct)
- Crispr associated protein cas11b (14 kDa, Protein from Synechocystis sp. PCC 6714)
- Rna (44-mer) (13 kDa, RNA from synthetic construct)
- Dna (5'-d(p*tp*cp*cp*ap*tp*gp*tp*tp*tp*ap*tp*cp*ap*cp*c)-3') (4 kDa, DNA from synthetic construct)
- Fruiting body developmental protein r-like protein (33 kDa, Protein from Synechocystis sp. PCC 6714)
- Type i-myxan crispr-associated protein cmx8 (63 kDa, Protein from Synechocystis sp. PCC 6714)
- Type i-myxan crispr-associated protein cas5/cmx5/devs (26 kDa, Protein from Synechocystis sp. PCC 6714)
- Dna (5'-d(p*ap*ap*ap*ap*ap*ap*ap*ap*ap*a)-3') (3 kDa, DNA from synthetic construct)
- Cryo-em structure of synechocystis sp. pcc6714 cascade bound to a pam-containing dsdna target presented as full r-loop formed state at 3.6 angstrom resolution (Complex from Synechocystis sp. PCC 6714)
Cryo-EM structure of Synechocystis sp. PCC6714 Cascade at 3.8 angstrom resolution
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 8h7q
Q-score: 0.346
To Be Published
- Type i-b cascade (Complex from Synechocystis sp. PCC 6714)
- Crispr associated protein cas5 (26 kDa, Protein from Synechocystis sp. PCC 6714)
- Crispr associated protein cas8 (33 kDa, Protein from Synechocystis sp. PCC 6714)
- Target dna (10 kDa, DNA from synthetic construct)
- Non target dna (3 kDa, DNA from synthetic construct)
- Crispr associated protein cas11b (14 kDa, Protein from Synechocystis sp. PCC 6714)
- Crispr rna (11 kDa, RNA from synthetic construct)
Cryo-EM structure of Cascade-DNA (P23) complex in type I-B CAST system
Resolution: 2.83 Å
EM Method: Single-particle
Fitted PDBs: 8fcj
Q-score: 0.534
Wang S, Gabel C, Siddique R, Klose T, Chang L
Cell (2023) 186 pp. 4204-4215.e19 [ Pubmed: 37557170 DOI: doi:10.1016/j.cell.2023.07.010 ]
- Type i-b crispr-associated protein cas7 (37 kDa, Protein from Nostoc sp. 'Peltigera membranacea cyanobiont' 210A)
- Type i-b crispr-associated protein cas8 (63 kDa, Protein from Nostoc sp. 'Peltigera membranacea cyanobiont' 210A)
- Type i-b crispr-associated protein cas11 (16 kDa, Protein from Nostoc sp. 'Peltigera membranacea cyanobiont' 210A)
- Target dna strand (19 kDa, DNA from synthetic construct)
- Type i-b crispr-associated protein cas5 (24 kDa, Protein from Nostoc sp. 'Peltigera membranacea cyanobiont' 210A)
- Non-target dna strand (20 kDa, DNA from synthetic construct)
- Type i-b crispr-associated protein cas6 (24 kDa, Protein from Nostoc sp. 'Peltigera membranacea cyanobiont' 210A)
- Ternary complex of cascade proteins, crrna and target dna with 23 bp recognized. (Complex from Peltigera membranacea)
- Rna (22 kDa, RNA from synthetic construct)
Cryo-EM structure of Cascade-DNA-TniQ-TnsC complex in type I-B CAST system
Resolution: 3.19 Å
EM Method: Single-particle
Fitted PDBs: 8fcu
Q-score: 0.393
Wang S, Gabel C, Siddique R, Klose T, Chang L
Cell (2023) 186 pp. 4204-4215.e19 [ Pubmed: 37557170 DOI: doi:10.1016/j.cell.2023.07.010 ]
- Type i-b crispr-associated protein cas7 (37 kDa, Protein from Nostoc sp. 'Peltigera membranacea cyanobiont' 210A)
- Target dna strand (26 kDa, DNA from synthetic construct)
- Tniq (37 kDa, Protein from Nostoc sp. 'Peltigera membranacea cyanobiont' 210A)
- Non-target dna strand (26 kDa, DNA from synthetic construct)
- Type i-b crispr-associated protein cas11 (16 kDa, Protein from Nostoc sp. 'Peltigera membranacea cyanobiont' 210A)
- Magnesium ion (24 Da, Ligand)
- Tnsc (43 kDa, Protein from Nostoc sp. 'Peltigera membranacea cyanobiont' 210A)
- Type i-b crispr-associated protein cas5 (24 kDa, Protein from Nostoc sp. 'Peltigera membranacea cyanobiont' 210A)
- Type i-b crispr-associated protein cas6 (24 kDa, Protein from Nostoc sp. 'Peltigera membranacea cyanobiont' 210A)
- Type i-myxan crispr-associated cas8a1/cmx1 (63 kDa, Protein from Nostoc sp. 'Peltigera membranacea cyanobiont' 210A)
- Cascade-dna-tniq-tnsc complex (Complex from Peltigera membranacea)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Rna (22 kDa, RNA from synthetic construct)
Cryo-EM structure of Cascade complex in type I-B CAST system
Resolution: 3.65 Å
EM Method: Single-particle
Fitted PDBs: 8fd2
Q-score: 0.23
Wang S, Gabel C, Siddique R, Klose T, Chang L
Cell (2023) 186 pp. 4204-4215.e19 [ Pubmed: 37557170 DOI: doi:10.1016/j.cell.2023.07.010 ]
- Type i-b crispr-associated protein cas7 (37 kDa, Protein from Nostoc sp. 'Peltigera membranacea cyanobiont' 210A)
- Type i-b crispr-associated protein cas8 (63 kDa, Protein from Nostoc sp. 'Peltigera membranacea cyanobiont' 210A)
- Type i-b crispr-associated protein cas11 (16 kDa, Protein from Nostoc sp. 'Peltigera membranacea cyanobiont' 210A)
- Type i-b crispr-associated protein cas6 (24 kDa, Protein from Nostoc sp. 'Peltigera membranacea cyanobiont' 210A)
- A type i-b crispr-associated protein cas5 (24 kDa, Protein from Nostoc sp. 'Peltigera membranacea cyanobiont' 210A)
- Cascade complex in type i-b cast system (Complex from Peltigera membranacea)
- Rna (22 kDa, RNA from synthetic construct)
Cryo-EM structure of Cascade-PAM complex in type I-B CAST system
Resolution: 3.12 Å
EM Method: Single-particle
Fitted PDBs: 8fd3
Q-score: 0.347
Wang S, Gabel C, Siddique R, Klose T, Chang L
Cell (2023) 186 pp. 4204-4215.e19 [ Pubmed: 37557170 DOI: doi:10.1016/j.cell.2023.07.010 ]
- Type i-b crispr-associated protein cas7 (37 kDa, Protein from Nostoc sp. 'Peltigera membranacea cyanobiont' 210A)
- Target dna strand (3 kDa, DNA from synthetic construct)
- Ternary complex of cascade proteins, crrna and pam only dna (Complex from Peltigera membranacea)
- Type i-b crispr-associated protein cas11 (16 kDa, Protein from Nostoc sp. 'Peltigera membranacea cyanobiont' 210A)
- Type i-b crispr-associated protein cas5 (24 kDa, Protein from Nostoc sp. 'Peltigera membranacea cyanobiont' 210A)
- Type i-b crispr-associated protein cas6 (24 kDa, Protein from Nostoc sp. 'Peltigera membranacea cyanobiont' 210A)
- Type i-myxan crispr-associated cas8a1/cmx1 (63 kDa, Protein from Nostoc sp. 'Peltigera membranacea cyanobiont' 210A)
- Non-target dna strand (13 kDa, DNA from synthetic construct)
- Rna (22 kDa, RNA from synthetic construct)
Cryo-EM structure of Cascade-DNA-TniQ-TnsC complex (composite) in type I-B CAST system
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 8ff4
Q-score: 0.417
Wang S, Gabel C, Siddique R, Klose T, Chang L
Cell (2023) 186 pp. 4204-4215.e19 [ Pubmed: 37557170 DOI: doi:10.1016/j.cell.2023.07.010 ]
- Type i-b crispr-associated protein cas7 (37 kDa, Protein from Nostoc sp. 'Peltigera membranacea cyanobiont' 210A)
- Target dna strand (26 kDa, DNA from synthetic construct)
- Type i-b crispr-associated protein cas8 (63 kDa, Protein from Nostoc sp. 'Peltigera membranacea cyanobiont' 210A)
- Tniq (37 kDa, Protein from Nostoc sp. 'Peltigera membranacea cyanobiont' 210A)
- Non-target dna strand (26 kDa, DNA from synthetic construct)
- Type i-b crispr-associated protein cas11 (16 kDa, Protein from Nostoc sp. 'Peltigera membranacea cyanobiont' 210A)
- Magnesium ion (24 Da, Ligand)
- Tnsc (43 kDa, Protein from Nostoc sp. 'Peltigera membranacea cyanobiont' 210A)
- Type i-b crispr-associated protein cas5 (24 kDa, Protein from Nostoc sp. 'Peltigera membranacea cyanobiont' 210A)
- Type i-b crispr-associated protein cas6 (24 kDa, Protein from Nostoc sp. 'Peltigera membranacea cyanobiont' 210A)
- Structure of cascade-dna-tniq-tnsc complex (composite) in type i-b cast system (Complex from Peltigera membranacea)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Rna (22 kDa, RNA from synthetic construct)
Cryo-EM structure of Cascade-DNA-fullRloop in type I-B CAST system
Resolution: 3.13 Å
EM Method: Single-particle
Fitted PDBs: 8ff5
Q-score: 0.372
Wang S, Gabel C, Siddique R, Klose T, Chang L
Cell (2023) 186 pp. 4204-4215.e19 [ Pubmed: 37557170 DOI: doi:10.1016/j.cell.2023.07.010 ]
- Type i-b crispr-associated protein cas7 (37 kDa, Protein from Nostoc sp. 'Peltigera membranacea cyanobiont' 210A)
- Type i-b crispr-associated protein cas8 (63 kDa, Protein from Nostoc sp. 'Peltigera membranacea cyanobiont' 210A)
- Type i-b crispr-associated protein cas11 (16 kDa, Protein from Nostoc sp. 'Peltigera membranacea cyanobiont' 210A)
- Ternary complex of cascade proteins, crrna and target dna with the complete r loop formed. (Complex from Peltigera membranacea)
- Nostoc sp. 'peltigera membranacea cyanobiont' 210a (24 kDa, Protein from Peltigera membranacea)
- Target dna strand (19 kDa, DNA from synthetic construct)
- Non-target dna strand (20 kDa, DNA from synthetic construct)
- Type i-b crispr-associated protein cas6 (24 kDa, Protein from Nostoc sp. 'Peltigera membranacea cyanobiont' 210A)
- Rna (22 kDa, RNA from synthetic construct)
Structure of type I-D Cascade bound to a dsDNA target
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7sba
Q-score: 0.453
Schwartz EA, McBride TM, Bravo JPK, Wrapp D, Fineran PC, Fagerlund RD, Taylor DW
Nat Commun (2022) 13 pp. 2829-2829 [ DOI: doi:10.1038/s41467-022-30402-8 Pubmed: 35595728 ]
- Crrna (13 kDa, RNA from Synechocystis sp. PCC 6803)
- Cas10d (112 kDa, Protein from Synechocystis sp. PCC 6803)
- Dna non-target strand (3 kDa, DNA from synthetic construct)
- Cas7d (36 kDa, Protein from Synechocystis sp. PCC 6803)
- Cas11d (17 kDa, Protein from Synechocystis sp. PCC 6803)
- Dna target strand (4 kDa, DNA from synthetic construct)
- Type i-d cascade bound to a dsdna target (480 kDa, Complex)
- Cas5d (28 kDa, Protein from Synechocystis sp. PCC 6803)