CRISPR-Cas type III-D effector complex bound to a self-target RNA in the pre-cleavage state
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 8s9v
Q-score: 0.561
Schwartz EA, Bravo JPK, Ahsan M, Macias LA, McCafferty CL, Dangerfield TL, Walker JN, Brodbelt JS, Palermo G, Fineran PC, Fagerlund RD, Taylor DW
Nat Commun (2024) 15 pp. 3324-3324 [ DOI: doi:10.1038/s41467-024-47506-y Pubmed: 38637512 ]
- Crispr-cas type iii-d effector complex (332 kDa, Complex from Synechocystis sp. PCC 6803)
- Magnesium ion (24 Da, Ligand)
- Cas10 (64 kDa, Protein from Synechocystis sp. PCC 6803)
- Cas7-cas5-cas11 (87 kDa, Protein from Synechocystis sp. PCC 6803)
- Self-target rna (19 kDa, RNA from Synechocystis sp. PCC 6803)
- Tigr03986 family crispr-associated ramp protein (90 kDa, Protein from Synechocystis sp. PCC 6803)
- Cas7-2x (56 kDa, Protein from Synechocystis sp. PCC 6803)
- Tigr03984 family crispr-associated protein (21 kDa, Protein from Synechocystis sp. PCC 6803)
- Crispr rna (11 kDa, RNA from Synechocystis sp. PCC 6803)
- Water (18 Da, Ligand)
Cryo-EM structure of Cascade-DNA-TniQ-TnsC complex in type I-B CAST system
Resolution: 3.19 Å
EM Method: Single-particle
Fitted PDBs: 8fcu
Q-score: 0.393
Wang S, Gabel C, Siddique R, Klose T, Chang L
Cell (2023) 186 pp. 4204-4215.e19 [ Pubmed: 37557170 DOI: doi:10.1016/j.cell.2023.07.010 ]
- Type i-b crispr-associated protein cas7 (37 kDa, Protein from Nostoc sp. 'Peltigera membranacea cyanobiont' 210A)
- Target dna strand (26 kDa, DNA from synthetic construct)
- Tniq (37 kDa, Protein from Nostoc sp. 'Peltigera membranacea cyanobiont' 210A)
- Non-target dna strand (26 kDa, DNA from synthetic construct)
- Type i-b crispr-associated protein cas11 (16 kDa, Protein from Nostoc sp. 'Peltigera membranacea cyanobiont' 210A)
- Magnesium ion (24 Da, Ligand)
- Tnsc (43 kDa, Protein from Nostoc sp. 'Peltigera membranacea cyanobiont' 210A)
- Type i-b crispr-associated protein cas5 (24 kDa, Protein from Nostoc sp. 'Peltigera membranacea cyanobiont' 210A)
- Type i-b crispr-associated protein cas6 (24 kDa, Protein from Nostoc sp. 'Peltigera membranacea cyanobiont' 210A)
- Type i-myxan crispr-associated cas8a1/cmx1 (63 kDa, Protein from Nostoc sp. 'Peltigera membranacea cyanobiont' 210A)
- Cascade-dna-tniq-tnsc complex (Complex from Peltigera membranacea)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Rna (22 kDa, RNA from synthetic construct)
Cryo-EM structure of Cascade-DNA-TniQ-TnsC complex (composite) in type I-B CAST system
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 8ff4
Q-score: 0.417
Wang S, Gabel C, Siddique R, Klose T, Chang L
Cell (2023) 186 pp. 4204-4215.e19 [ Pubmed: 37557170 DOI: doi:10.1016/j.cell.2023.07.010 ]
- Type i-b crispr-associated protein cas7 (37 kDa, Protein from Nostoc sp. 'Peltigera membranacea cyanobiont' 210A)
- Target dna strand (26 kDa, DNA from synthetic construct)
- Type i-b crispr-associated protein cas8 (63 kDa, Protein from Nostoc sp. 'Peltigera membranacea cyanobiont' 210A)
- Tniq (37 kDa, Protein from Nostoc sp. 'Peltigera membranacea cyanobiont' 210A)
- Non-target dna strand (26 kDa, DNA from synthetic construct)
- Type i-b crispr-associated protein cas11 (16 kDa, Protein from Nostoc sp. 'Peltigera membranacea cyanobiont' 210A)
- Magnesium ion (24 Da, Ligand)
- Tnsc (43 kDa, Protein from Nostoc sp. 'Peltigera membranacea cyanobiont' 210A)
- Type i-b crispr-associated protein cas5 (24 kDa, Protein from Nostoc sp. 'Peltigera membranacea cyanobiont' 210A)
- Type i-b crispr-associated protein cas6 (24 kDa, Protein from Nostoc sp. 'Peltigera membranacea cyanobiont' 210A)
- Structure of cascade-dna-tniq-tnsc complex (composite) in type i-b cast system (Complex from Peltigera membranacea)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Rna (22 kDa, RNA from synthetic construct)