Cryo-EM structure of the FB1-bound Lac1-Lip1 complex
Resolution: 3.07 Å
EM Method: Single-particle
Fitted PDBs: 8y2m
Q-score: 0.499
Zhang Z, Fang Q, Xie T, Gong X
Structure (2024) 32 pp. 1419-1428.e4 [ Pubmed: 38964337 DOI: doi:10.1016/j.str.2024.06.002 ]
- Ceramide synthase subunit lip1 (17 kDa, Protein from Saccharomyces cerevisiae S288C)
- Fb1-bound lac1-lip1 complex (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Hexacosanoic acid (396 Da, Ligand)
- Ceramide synthase lac1 (50 kDa, Protein from Saccharomyces cerevisiae S288C)
- (2~{r})-2-[2-[(5~{r},6~{r},7~{s},9~{s},11~{r},16~{r},18~{s},19~{s})-19-azanyl-6-[(3~{r})-3-carboxy-5-oxidanyl-5-oxidanylidene-pentanoyl]oxy-5,9-dimethyl-11,16,18-tris(oxidanyl)icosan-7-yl]oxy-2-oxidanylidene-ethyl]butanedioic acid (721 Da, Ligand)
- (4s,7r)-4-hydroxy-n,n,n-trimethyl-9-oxo-7-[(palmitoyloxy)methyl]-3,5,8-trioxa-4-phosphahexacosan-1-aminium 4-oxide (763 Da, Ligand)
Cryo-EM structure of the apo Lac1-Lip1 complex
Resolution: 3.19 Å
EM Method: Single-particle
Fitted PDBs: 8y2n
Q-score: 0.492
Zhang Z, Fang Q, Xie T, Gong X
Structure (2024) 32 pp. 1419-1428.e4 [ Pubmed: 38964337 DOI: doi:10.1016/j.str.2024.06.002 ]
- Lac1-lip1 complex (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Ceramide synthase subunit lip1 (17 kDa, Protein from Saccharomyces cerevisiae S288C)
- (4s,7r)-4-hydroxy-n,n,n-trimethyl-9-oxo-7-[(palmitoyloxy)methyl]-3,5,8-trioxa-4-phosphahexacosan-1-aminium 4-oxide (763 Da, Ligand)
- Ceramide synthase lac1 (50 kDa, Protein from Saccharomyces cerevisiae S288C)
Cryo-EM structure of the C26-FB1-bound Lac1-Lip1 complex
Resolution: 2.86 Å
EM Method: Single-particle
Fitted PDBs: 8zb1
Q-score: 0.556
Zhang Z, Fang Q, Xie T, Gong X
Structure (2024) 32 pp. 1419-1428.e4 [ Pubmed: 38964337 DOI: doi:10.1016/j.str.2024.06.002 ]
- (2~{r})-2-[2-[(5~{r},6~{r},7~{s},9~{s},11~{r},16~{r},18~{s},19~{s})-6-[(3~{r})-3-carboxy-5-oxidanyl-5-oxidanylidene-pentanoyl]oxy-19-(hexacosanoylamino)-5,9-dimethyl-11,16,18-tris(oxidanyl)icosan-7-yl]oxy-2-oxidanylidene-ethyl]butanedioic acid (1 kDa, Ligand)
- Lac1-lip1 complex (Complex from Saccharomyces cerevisiae (strain YJM789))
- Ceramide synthase subunit lip1 (17 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Ceramide synthase lac1 (50 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- (4s,7r)-4-hydroxy-n,n,n-trimethyl-9-oxo-7-[(palmitoyloxy)methyl]-3,5,8-trioxa-4-phosphahexacosan-1-aminium 4-oxide (763 Da, Ligand)
Cryo-EM structure of the apo yeast Ceramide Synthase
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 8qtn
Q-score: 0.513
Schafer JH, Clausmeyer L, Korner C, Esch BM, Wolf VN, Sapia J, Ahmed Y, Walter S, Vanni S, Januliene D, Moeller A, Frohlich F
Nat Struct Mol Biol (2024) [ Pubmed: 39528796 DOI: doi:10.1038/s41594-024-01415-2 ]
- Ceramide synthase subunit lip1 (15 kDa, Protein from Saccharomyces cerevisiae)
- Ceramide synthase lag1 (37 kDa, Protein from Saccharomyces cerevisiae)
- [(2s)-1-hexadecanoyloxy-3-[hydroxy-[(2s,3r,5s,6r)-2,3,4,5,6-pentahydroxycyclohexyl]oxy-phosphoryl]oxy-propan-2-yl] heptadecanoate (825 Da, Ligand)
- Ceramide synthase lac1 (36 kDa, Protein from Saccharomyces cerevisiae)
- 1,2-distearoyl-sn-glycerophosphoethanolamine (748 Da, Ligand)
- Ammonium ion (18 Da, Ligand)
- Hexacosanoic acid (396 Da, Ligand)
- Heterotetrameric complex of yeast ceramide synthase with lag1, lac1 and lip1 (140 kDa, Complex from Saccharomyces cerevisiae)
Cryo-EM structure of the FB-bound yeast Ceramide Synthase
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8qtr
Q-score: 0.464
Schafer JH, Clausmeyer L, Korner C, Esch BM, Wolf VN, Sapia J, Ahmed Y, Walter S, Vanni S, Januliene D, Moeller A, Frohlich F
Nat Struct Mol Biol (2024) [ Pubmed: 39528796 DOI: doi:10.1038/s41594-024-01415-2 ]
- Ceramide synthase lac1 (35 kDa, Protein from Saccharomyces cerevisiae)
- Macrofusine (721 Da, Ligand)
- Ceramide synthase lag1 (37 kDa, Protein from Saccharomyces cerevisiae)
- [(2s)-1-hexadecanoyloxy-3-[hydroxy-[(2s,3r,5s,6r)-2,3,4,5,6-pentahydroxycyclohexyl]oxy-phosphoryl]oxy-propan-2-yl] heptadecanoate (825 Da, Ligand)
- Ammonium ion (18 Da, Ligand)
- 1,2-distearoyl-sn-glycerophosphoethanolamine (748 Da, Ligand)
- Ceramide synthase subunit lip1 (15 kDa, Protein from Saccharomyces cerevisiae)
- Hexacosanoic acid (396 Da, Ligand)
- Heterotetrameric complex of yeast ceramide synthase with lag1, lac1 and lip1 (140 kDa, Complex from Saccharomyces cerevisiae)
Cryo-EM structure of the C26-CoA-bound Lac1-Lip1 complex
Resolution: 3.09 Å
EM Method: Single-particle
Fitted PDBs: 8izd
Q-score: 0.562
Xie T, Fang Q, Zhang Z, Wang Y, Dong F, Gong X
EMBO J (2023) 42 pp. e114889-e114889 [ DOI: doi:10.15252/embj.2023114889 Pubmed: 37953642 ]
- Lac1-lip1 complex (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Ceramide synthase subunit lip1 (17 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Ceramide synthase lac1 (50 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- (4s,7r)-4-hydroxy-n,n,n-trimethyl-9-oxo-7-[(palmitoyloxy)methyl]-3,5,8-trioxa-4-phosphahexacosan-1-aminium 4-oxide (763 Da, Ligand)
- Hexacosanoyl-coa (1 kDa, Ligand)
Cryo-EM structure of the Lac1-Lip1 (Lip1-S74F) complex
Resolution: 3.85 Å
EM Method: Single-particle
Fitted PDBs: 8izf
Q-score: 0.396
Xie T, Fang Q, Zhang Z, Wang Y, Dong F, Gong X
EMBO J (2023) 42 pp. e114889-e114889 [ DOI: doi:10.15252/embj.2023114889 Pubmed: 37953642 ]
- Ceramide synthase lac1 (49 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Ceramide synthase subunit lip1 (19 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- (4s,7r)-4-hydroxy-n,n,n-trimethyl-9-oxo-7-[(palmitoyloxy)methyl]-3,5,8-trioxa-4-phosphahexacosan-1-aminium 4-oxide (763 Da, Ligand)
- Lac1-lip1 (lip1-s74f) complex (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))