Cholecystokinin 1 receptor (CCK1R) Y140A mutant, Gq chimera (mGsqi) complex
Harikumar KG, Zhao P, Cary BP, Xu X, Desai AJ, Dong M, Mobbs JI, Toufaily C, Furness SGB, Christopoulos A, Belousoff MJ, Wootten D, Sexton PM, Miller LJ
PLoS Biol (2024) 22 pp. e3002673-e3002673 [ Pubmed: 39083706 DOI: doi:10.1371/journal.pbio.3002673 ]
- Cholecystokinin receptor type a (47 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1,guanine nucleotide-binding protein g(s) subunit alpha isoforms short (29 kDa, Protein from Homo sapiens)
- Complex of cck1r (y140a mutant) bound to minigs (gq, gi chimera), g-beta1, g-gamma-2 and cck8s. (150 kDa, Complex from Homo sapiens)
- Cholecystokinin-8 (1 kDa, Protein from Homo sapiens)
- Amino group (16 Da, Ligand)
Cholecystokinin 1 receptor (CCK1R) sterol 7M mutant, Gq chimera (mGsqi) complex
Harikumar KG, Zhao P, Cary BP, Xu X, Desai AJ, Dong M, Mobbs JI, Toufaily C, Furness SGB, Christopoulos A, Belousoff MJ, Wootten D, Sexton PM, Miller LJ
PLoS Biol (2024) 22 pp. e3002673-e3002673 [ Pubmed: 39083706 DOI: doi:10.1371/journal.pbio.3002673 ]
- Cholecystokinin-8 (1 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms xlas (29 kDa, Protein from Homo sapiens)
- Complex of cck1r (sterol 7m mutant) bound to minigs (gq, gi chimera), g-beta1, g-gamma-2 and cck8s. (150 kDa, Complex from Homo sapiens)
- Cholecystokinin receptor type a (47 kDa, Protein from Homo sapiens)
Cryo-EM structure of an activated Cholecystokinin A receptor (CCKAR)-Gi complex
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7ezh
Q-score: 0.42
Liu Q, Yang D, Zhuang Y, Croll TI, Cai X, Dai A, He X, Duan J, Yin W, Ye C, Zhou F, Wu B, Zhao Q, Xu HE, Wang MW, Jiang Y
Nat Chem Biol (2021) 17 pp. 1238-1244 [ DOI: doi:10.1038/s41589-021-00841-3 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Scfv16 (26 kDa, Protein from synthetic construct)
- Cholecystokinin receptor type a (47 kDa, Protein from Homo sapiens)
- Cryo-em structure of an activated cholecystokinin a receptor (cckar)-gi complex (Complex from Homo sapiens)
- Cholecystokinin-8 (1 kDa, Protein from Homo sapiens)
Cryo-EM structure of an activated Cholecystokinin A receptor (CCKAR)-Gs complex
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7ezk
Q-score: 0.435
Liu Q, Yang D, Zhuang Y, Croll TI, Cai X, Dai A, He X, Duan J, Yin W, Ye C, Zhou F, Wu B, Zhao Q, Xu HE, Wang MW, Jiang Y
Nat Chem Biol (2021) 17 pp. 1238-1244 [ DOI: doi:10.1038/s41589-021-00841-3 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Chimera of guanine nucleotide-binding protein g(i) subunit alpha-1 and guanine nucleotide-binding protein g(s) subunit alpha isoforms short (41 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Cholecystokinin receptor type a (47 kDa, Protein from Homo sapiens)
- Cryo-em structure of an activated cholecystokinin a receptor (cckar)-gs complex (Complex from Homo sapiens)
- Cholecystokinin-8 (1 kDa, Protein from Homo sapiens)
Cryo-EM structure of an activated Cholecystokinin A receptor (CCKAR)-Gq complex
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7ezm
Q-score: 0.486
Liu Q, Yang D, Zhuang Y, Croll TI, Cai X, Dai A, He X, Duan J, Yin W, Ye C, Zhou F, Wu B, Zhao Q, Xu HE, Wang MW, Jiang Y
Nat Chem Biol (2021) 17 pp. 1238-1244 [ DOI: doi:10.1038/s41589-021-00841-3 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Fusion protein of guanine nucleotide-binding protein g(i) subunit alpha-1 and guanine nucleotide-binding protein g(q) subunit alpha-q (41 kDa, Protein from Homo sapiens)
- Cryo-em structure of an activated cholecystokinin a receptor (cckar)-gq complex (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Scfv16 (26 kDa, Protein from synthetic construct)
- Cholecystokinin receptor type a (47 kDa, Protein from Homo sapiens)
- Cholecystokinin-8 (1 kDa, Protein from Homo sapiens)
Human Cholecystokinin 1 receptor (CCK1R) Gs complex
Resolution: 1.95 Å
EM Method: Single-particle
Fitted PDBs: 7mbx
Q-score: 0.619
Mobbs JI, Belousoff MJ, Harikumar KG, Piper SJ, Xu X, Furness SGB, Venugopal H, Christopoulos A, Danev R, Wootten D, Thal DM, Miller LJ, Sexton PM
PLoS Biol (2021) 19 pp. e3001295-e3001295 [ Pubmed: 34086670 DOI: doi:10.1371/journal.pbio.3001295 ]
- Cholecystokinin receptor type a (48 kDa, Protein from Homo sapiens)
- Cholecystokinin-8 (1 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Cholecystokinin receptor type a (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Guanine nucleotide-binding protein g(s) subunits alpha and gamma (Complex from Homo sapiens)
- Cholecystokinin-8 (Complex from Homo sapiens)
- Cholesterol hemisuccinate (486 Da, Ligand)
- Nanobody35 (15 kDa, Protein from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (Complex from Rattus norvegicus)
- Nanobody35 (Complex from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Rattus norvegicus)
- Cck1r/cck-8/gas complex (140 MDa, Complex)
Human Cholecystokinin 1 receptor (CCK1R) Gq chimera (mGsqi) complex
Resolution: 2.44 Å
EM Method: Single-particle
Fitted PDBs: 7mby
Q-score: 0.413
Mobbs JI, Belousoff MJ, Harikumar KG, Piper SJ, Xu X, Furness SGB, Venugopal H, Christopoulos A, Danev R, Wootten D, Thal DM, Miller LJ, Sexton PM
PLoS Biol (2021) 19 pp. e3001295-e3001295 [ Pubmed: 34086670 DOI: doi:10.1371/journal.pbio.3001295 ]
- Cholecystokinin-8 (1 kDa, Protein from Homo sapiens)
- Cck-8 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1,guanine nucleotide-binding protein g(s) subunit alpha isoforms short,with certain residues mutated to match guanine nucleotide-binding protein g(q) subunit (29 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Cholesterol hemisuccinate (486 Da, Ligand)
- G protein subunit beta-1 (Complex from Rattus norvegicus)
- G(sqi)-alpha/g protein subunit gamma-2 (Complex from Homo sapiens)
- Cck1r (Complex from Homo sapiens)
- Scfv16 (Complex from Mus musculus)
- Cholecystokinin receptor type a (47 kDa, Protein from Homo sapiens)
- Cck1r/cck-8/mgsqi complex (150 MDa, Complex)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Rattus norvegicus)