Cryo-EM structure of the human mPSF with PAPOA C-terminus peptide (PAPOAc)
Resolution: 2.79 Å
EM Method: Single-particle
Fitted PDBs: 8r8r
Q-score: 0.578
Todesca S, Sandmeir F, Keidel A, Conti E
RNA (2024) 30 pp. 795-806 [ Pubmed: 38538052 DOI: doi:10.1261/rna.079915.123 ]
- Zinc ion (65 Da, Ligand)
- Cdna flj50397, highly similar to poly(a) polymerase alpha (2 kDa, Protein from Homo sapiens)
- Pre-mrna 3' end processing protein wdr33 (47 kDa, Protein from Homo sapiens)
- Mpsf with papoa c-terminus peptide (papoac) (Complex from Homo sapiens)
- Rna (5'-r(p*ap*ap*up*ap*ap*a)-3') (1 kDa, RNA from Homo sapiens)
- Cleavage and polyadenylation specificity factor subunit 4 (31 kDa, Protein from Homo sapiens)
- Cleavage and polyadenylation specificity factor subunit 1 (161 kDa, Protein from Homo sapiens)
CRYO-EM STRUCTURE OF the human MPSF IN COMPLEX WITH THE AUUAAA poly(A) signal
Resolution: 2.53 Å
EM Method: Single-particle
Fitted PDBs: 8e3i
Q-score: 0.632
Gutierrez PA, Wei J, Sun Y, Tong L
RNA (2022) 28 pp. 1534-1541 [ Pubmed: 36130077 DOI: doi:10.1261/rna.079322.122 ]
- Zinc ion (65 Da, Ligand)
- Cleavage and polyadenylation specificity factor subunit 4 (27 kDa, Protein from Homo sapiens)
- Rna (5'-r(p*cp*ap*up*up*ap*ap*ap*cp*ap*ap*c)-3') (3 kDa, RNA from Homo sapiens)
- Human mpsf-auuaaa rna complex (Complex from Homo sapiens)
- Pre-mrna 3' end processing protein wdr33 (65 kDa, Protein from Homo sapiens)
- Cleavage and polyadenylation specificity factor subunit 1 (161 kDa, Protein from Homo sapiens)
CRYO-EM STRUCTURE OF the human MPSF
Resolution: 2.68 Å
EM Method: Single-particle
Fitted PDBs: 8e3q
Q-score: 0.588
Gutierrez PA, Wei J, Sun Y, Tong L
RNA (2022) 28 pp. 1534-1541 [ Pubmed: 36130077 DOI: doi:10.1261/rna.079322.122 ]
- Zinc ion (65 Da, Ligand)
- Cleavage and polyadenylation specificity factor subunit 4 (27 kDa, Protein from Homo sapiens)
- Pre-mrna 3' end processing protein wdr33 (65 kDa, Protein from Homo sapiens)
- Cleavage and polyadenylation specificity factor subunit 1 (161 kDa, Protein from Homo sapiens)
- Human mpsf (Complex from Homo sapiens)
Human RBBP6-bound CPSF complex
Schmidt M, Kluge F, Sandmeir F, Kuhn U, Schafer P, Tuting C, Ihling C, Conti E, Wahle E
Genes Dev (2022) 36 pp. 195-209 [ Pubmed: 35177537 DOI: doi:10.1101/gad.349217.121 ]
- Cleavage and polyadenylation specificity factor subunit 4 (Protein from Homo sapiens)
- Cleavage stimulation factor subunit 2 (Protein from Homo sapiens)
- Cleavage and polyadenylation specificity factor subunit 3 (Protein from Homo sapiens)
- Pre-mrna 3' end processing protein wdr33 (Protein from Homo sapiens)
- Complex of rbbp6-bound cpsf (Complex from Homo sapiens)
- Cleavage and polyadenylation specificity factor subunit 1 (Protein from Homo sapiens)
- E3 ubiquitin-protein ligase rbbp6 (Protein from Homo sapiens)
- Symplekin (Protein from Homo sapiens)
- Cleavage and polyadenylation specificity factor subunit 2 (Protein from Homo sapiens)
Cryo-EM structure of human CPSF160-WDR33-CPSF30-CPSF100 PIM complex
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 6urg
Q-score: 0.543
Zhang Y, Sun Y, Shi Y, Walz T, Tong L
Mol Cell (2020) 77 pp. 800- [ DOI: doi:10.1016/j.molcel.2019.11.005 Pubmed: 31810758 ]
- Zinc ion (65 Da, Ligand)
- The complex of human mpsf-cpsf100 pim (Complex from Homo sapiens)
- Cleavage and polyadenylation specificity factor subunit 4 (28 kDa, Protein from Homo sapiens)
- Pre-mrna 3' end processing protein wdr33 (67 kDa, Protein from Homo sapiens)
- Cleavage and polyadenylation specificity factor subunit 2 (88 kDa, Protein from Homo sapiens)
- Cleavage and polyadenylation specificity factor subunit 1 (161 kDa, Protein from Homo sapiens)
Cryo-EM structure of human CPSF160-WDR33-CPSF30-PAS RNA-CstF77 complex
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 6uro
Q-score: 0.348
Zhang Y, Sun Y, Shi Y, Walz T, Tong L
Mol Cell (2020) 77 pp. 800- [ DOI: doi:10.1016/j.molcel.2019.11.005 Pubmed: 31810758 ]
- Zinc ion (65 Da, Ligand)
- Cleavage stimulation factor subunit 3 (83 kDa, Protein from Homo sapiens)
- Cleavage and polyadenylation specificity factor subunit 4 (28 kDa, Protein from Homo sapiens)
- The complex of human mpsf-pas rna-cstf (Complex from Homo sapiens)
- Pre-mrna 3' end processing protein wdr33 (67 kDa, Protein from Homo sapiens)
- Pas rna (14 kDa, RNA from Macaca mulatta polyomavirus 1)
- Cleavage and polyadenylation specificity factor subunit 1 (161 kDa, Protein from Homo sapiens)
Clerici M, Faini M, Muckenfuss LM, Aebersold R, Jinek M
Nat Struct Mol Biol (2018) 25 pp. 135-138 [ DOI: doi:10.1038/s41594-017-0020-6 Pubmed: 29358758 ]
- Cleavage and polyadenylation specificity factor subunit 4 (20 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Rna (5'-r(p*ap*ap*up*ap*ap*ap*gp*g)-3') (3 kDa, RNA from unidentified adenovirus)
- Pre-mrna 3' end processing protein wdr33 (47 kDa, Protein from Homo sapiens)
- Human cpsf160-wdr33-cpsf30 heterotrimer bound to the aauaaa motif of the polyadenylation signal (220 kDa, Complex)
- Cleavage and polyadenylation specificity factor subunit 1 (161 kDa, Protein from Homo sapiens)
- Human cpsf160-wdr33-cpsf30 heterotrimer (Complex from Homo sapiens)
- Aauaaa motif of the polyadenylation signal (Complex from unidentified adenovirus)
Cryo-EM structure of human CPSF-160-WDR33-CPSF-30-PAS RNA complex at 3.4 A resolution
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 6dnh
Q-score: 0.512
Sun Y, Zhang Y, Hamilton K, Manley JL, Shi Y, Walz T, Tong L
PNAS (2018) 115 pp. E1419-E1428 [ Pubmed: 29208711 DOI: doi:10.1073/pnas.1718723115 ]
- Zinc ion (65 Da, Ligand)
- Cleavage and polyadenylation specificity factor subunit 4 (27 kDa, Protein from Homo sapiens)
- Quaternary complex of human cpsf-160-wdr33-cpsf-30 with aauaaa polyadenylation signal (255 kDa, Complex)
- Cpsf-30 (27 kDa, Complex from Homo sapiens)
- Rna (5'-r(p*ap*ap*up*ap*ap*ap*c)-3') (5 kDa, RNA from Simian virus 40)
- Cleavage and polyadenylation specificity factor subunit 1 (161 kDa, Protein from Homo sapiens)
- Cpsf-160-wdr33 (225 kDa, Complex from Homo sapiens)
- Pre-mrna 3' end processing protein wdr33 (67 kDa, Protein from Homo sapiens)
- Aauaaa polyadenylation signal (5 kDa, Complex from Simian virus 40)
Cryo-EM structure of human CPSF-160-WDR33 complex at 3.36 A resolution
Resolution: 3.36 Å
EM Method: Single-particle
Fitted PDBs: 6bly
Q-score: 0.513
Sun Y, Zhang Y, Hamilton K, Manley JL, Shi Y, Walz T, Tong L
PNAS (2018) 115 pp. E1419-E1428 [ Pubmed: 29208711 DOI: doi:10.1073/pnas.1718723115 ]
- Binary complex of human cpsf-160-wdr33 (225 kDa, Complex from Homo sapiens)
- Cleavage and polyadenylation specificity factor subunit 1 (161 kDa, Protein from Homo sapiens)
- Pre-mrna 3' end processing protein wdr33 (67 kDa, Protein from Homo sapiens)
Cryo-EM structure of human CPSF-160-WDR33 complex at 3.8 A resolution
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 6bm0
Q-score: 0.431
Sun Y, Zhang Y, Hamilton K, Manley JL, Shi Y, Walz T, Tong L
PNAS (2018) 115 pp. E1419-E1428 [ Pubmed: 29208711 DOI: doi:10.1073/pnas.1718723115 ]
- Binary complex of human cpsf-160-wdr33 (225 kDa, Complex from Homo sapiens)
- Cleavage and polyadenylation specificity factor subunit 1 (161 kDa, Protein from Homo sapiens)
- Pre-mrna 3' end processing protein wdr33 (67 kDa, Protein from Homo sapiens)