Type III-B Cmr-beta Cryo-EM structure of the Apo state
Resolution: 2.75 Å
EM Method: Single-particle
Fitted PDBs: 6s6b
Q-score: 0.456
Sofos N, Feng M, Stella S, Pape T, Fuglsang A, Lin J, Huang Q, Li Y, She Q, Montoya G
Mol Cell (2020) 79 pp. 741-757.e7 [ DOI: doi:10.1016/j.molcel.2020.07.008 Pubmed: 32730741 ]
- Zinc ion (65 Da, Ligand)
- Crispr-associated ramp protein, cmr4 family (32 kDa, Protein from Sulfolobus islandicus (strain REY15A))
- Crispr-associated ramp protein, cmr6 family (33 kDa, Protein from Sulfolobus islandicus (strain REY15A))
- Crispr-associated protein, cmr2 family (120 kDa, Protein from Sulfolobus islandicus (strain REY15A))
- Crispr-associated protein cmrx (19 kDa, Protein from Sulfolobus islandicus (strain REY15A))
- Crispr-associated protein, cmr3 family (36 kDa, Protein from Sulfolobus islandicus (strain REY15A))
- Crrna (51-mer) (16 kDa, RNA from Sulfolobus islandicus REY15A)
- Cmr1 (55 kDa, Protein from Sulfolobus islandicus REY15A)
- Crispr-associated protein, cmr5 family (17 kDa, Protein from Sulfolobus islandicus (strain REY15A))
- Type iii-b cmr-beta binary complex, crrna-bound (900 kDa, Complex from Sulfolobus islandicus REY15A)
Cryo-EM structure of the Type III-B Cmr-beta bound to cognate target RNA and AMPPnP, state 1
Resolution: 2.41 Å
EM Method: Single-particle
Fitted PDBs: 6s8b
Q-score: 0.48
Sofos N, Feng M, Stella S, Pape T, Fuglsang A, Lin J, Huang Q, Li Y, She Q, Montoya G
Mol Cell (2020) 79 pp. 741-757.e7 [ DOI: doi:10.1016/j.molcel.2020.07.008 Pubmed: 32730741 ]
- Zinc ion (65 Da, Ligand)
- Crispr-associated ramp protein, cmr6 family (33 kDa, Protein from Sulfolobus islandicus (strain REY15A))
- Type iii-b cmr-beta ternary complex, cognate target rna and amppnp bound (900 kDa, Complex from Sulfolobus islandicus REY15A)
- Crispr-associated protein cmrx (19 kDa, Protein from Sulfolobus islandicus (strain REY15A))
- Crispr-associated protein, cmr2 family (120 kDa, Protein from Sulfolobus islandicus (strain REY15A))
- Crispr-associated protein, cmr3 family (36 kDa, Protein from Sulfolobus islandicus (strain REY15A))
- Crispr-associated ramp protein, cmr4 family (32 kDa, Protein from Sulfolobus islandicus (strain REY15A))
- Crrna (16 kDa, RNA from Sulfolobus islandicus REY15A)
- Cognate target rna (46-mer) (14 kDa, RNA from Sulfolobus islandicus REY15A)
- Cmr1 (55 kDa, Protein from Sulfolobus islandicus REY15A)
- Manganese (ii) ion (54 Da, Ligand)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Crispr-associated protein, cmr5 family (17 kDa, Protein from Sulfolobus islandicus (strain REY15A))
Cryo-EM structure of the type III-B Cmr-beta complex bound to non-cognate target RNA
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 6s8e
Q-score: 0.397
Sofos N, Feng M, Stella S, Pape T, Fuglsang A, Lin J, Huang Q, Li Y, She Q, Montoya G
Mol Cell (2020) 79 pp. 741-757.e7 [ DOI: doi:10.1016/j.molcel.2020.07.008 Pubmed: 32730741 ]
- Crispr-associated protein, cmr3 family (36 kDa, Protein from Sulfolobus islandicus REY15A)
- Crispr-associated ramp protein, cmr6 family (33 kDa, Protein from Sulfolobus islandicus REY15A)
- Zinc ion (65 Da, Ligand)
- Non-cognate target rna (15 kDa, RNA from synthetic construct)
- Crispr-associated ramp protein, cmr4 family (32 kDa, Protein from Sulfolobus islandicus REY15A)
- Crrna (16 kDa, RNA from Sulfolobus islandicus REY15A)
- Type iii-b cmr-beta complex bound to non-cognate target rna (900 kDa, Complex from Sulfolobus islandicus REY15A)
- Crispr-associated protein cmr7 (19 kDa, Protein from Sulfolobus islandicus REY15A)
- Cmr1,crispr-associated ramp protein, cmr1 family,crispr-associated ramp protein, cmr1 family (55 kDa, Protein from Sulfolobus islandicus REY15A)
- Crispr-associated protein, cmr2 family (120 kDa, Protein from Sulfolobus islandicus REY15A)
- Crispr-associated protein, cmr5 family (17 kDa, Protein from Sulfolobus islandicus REY15A)
Cryo-EM structure of the Type III-B Cmr-beta bound to cognate target RNA and AMPPnP, state 2
Resolution: 2.68 Å
EM Method: Single-particle
Fitted PDBs: 6s91
Q-score: 0.463
Sofos N, Feng M, Stella S, Pape T, Fuglsang A, Lin J, Huang Q, Li Y, She Q, Montoya G
Mol Cell (2020) 79 pp. 741-757.e7 [ DOI: doi:10.1016/j.molcel.2020.07.008 Pubmed: 32730741 ]
- Crispr-associated proteins cmr5, cmr3, cmr1, cmr2, cmr7 (Complex from Sulfolobus islandicus REY15A)
- Zinc ion (65 Da, Ligand)
- Cognate target rna (14 kDa, RNA from synthetic construct)
- Crispr-associated ramp protein, cmr6 family (33 kDa, Protein from Sulfolobus islandicus (strain REY15A))
- Crispr-associated protein, cmr2 family (120 kDa, Protein from Sulfolobus islandicus (strain REY15A))
- Crispr-associated protein cmrx (19 kDa, Protein from Sulfolobus islandicus (strain REY15A))
- Crispr-associated protein, cmr3 family (36 kDa, Protein from Sulfolobus islandicus (strain REY15A))
- Crispr-associated ramp protein, cmr4 family (32 kDa, Protein from Sulfolobus islandicus (strain REY15A))
- Cognate target rna (Complex from synthetic construct)
- Crrna (48-mer) (16 kDa, RNA from Sulfolobus islandicus REY15A)
- Type iii-b cmr-beta ternary complex, cognate target rna and amppnp bound (900 kDa, Complex)
- Cmr1,crispr-associated ramp protein, cmr1 family (55 kDa, Protein from Sulfolobus islandicus (strain REY15A))
- Manganese (ii) ion (54 Da, Ligand)
- Crispr-associated ramp proteins cmr4 and cmr6, and crrna (Complex from Sulfolobus islandicus REY15A)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Crispr-associated protein, cmr5 family (17 kDa, Protein from Sulfolobus islandicus (strain REY15A))
Resolution: 3.14 Å
EM Method: Single-particle
Fitted PDBs: 6sh8
Q-score: 0.437
Sofos N, Feng M, Stella S, Pape T, Fuglsang A, Lin J, Huang Q, Li Y, She Q, Montoya G
Mol Cell (2020) 79 pp. 741-757.e7 [ DOI: doi:10.1016/j.molcel.2020.07.008 Pubmed: 32730741 ]
- Crispr-associated protein, cmr3 family (Complex from Sulfolobus islandicus REY15A)
- Crispr-associated ramp protein, cmr4 family (32 kDa, Protein from Sulfolobus islandicus REY15A)
- Cmr1,crispr-associated ramp protein, cmr1 family (55 kDa, Protein from Sulfolobus islandicus REY15A)
- Crispr-associated protein, cmr2 family (Complex from Sulfolobus islandicus REY15A)
- Crispr-associated protein cmrx (Complex from Sulfolobus islandicus REY15A)
- Cmr1,crispr-associated ramp protein, cmr1 family (Complex from Sulfolobus islandicus REY15A)
- Manganese (ii) ion (54 Da, Ligand)
- Crispr-associated protein cmrx (19 kDa, Protein from Sulfolobus islandicus REY15A)
- Cognate target rna (Complex from synthetic construct)
- Type iii-b cmr-beta ternary complex, cognate target rna and amppnp bound, in the presence of ssdna (900 kDa, Complex)
- Crrna (Complex from Sulfolobus islandicus REY15A)
- Crispr-associated protein, cmr5 family (Complex from Sulfolobus islandicus REY15A)
- Crispr-associated protein, cmr3 family (36 kDa, Protein from Sulfolobus islandicus REY15A)
- Crispr-associated ramp protein, cmr6 family (33 kDa, Protein from Sulfolobus islandicus REY15A)
- Cognate target rna (14 kDa, RNA from synthetic construct)
- Crrna (16 kDa, RNA from Sulfolobus islandicus REY15A)
- Crispr-associated protein, cmr2 family (120 kDa, Protein from Sulfolobus islandicus REY15A)
- Crispr-associated protein, cmr5 family (17 kDa, Protein from Sulfolobus islandicus REY15A)
- Zinc ion (65 Da, Ligand)
- Crispr-associated ramp protein, cmr6 family (Complex from Sulfolobus islandicus REY15A)
- Crispr-associated ramp protein, cmr4 family (Complex from Sulfolobus islandicus REY15A)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
Resolution: 3.07 Å
EM Method: Single-particle
Fitted PDBs: 6shb
Q-score: 0.442
Sofos N, Feng M, Stella S, Pape T, Fuglsang A, Lin J, Huang Q, Li Y, She Q, Montoya G
Mol Cell (2020) 79 pp. 741-757.e7 [ DOI: doi:10.1016/j.molcel.2020.07.008 Pubmed: 32730741 ]
- Crispr-associated protein, cmr3 family (Complex from Sulfolobus islandicus REY15A)
- Crispr-associated ramp protein, cmr4 family (32 kDa, Protein from Sulfolobus islandicus REY15A)
- Cmr1,crispr-associated ramp protein, cmr1 family (55 kDa, Protein from Sulfolobus islandicus REY15A)
- Crispr-associated protein, cmr2 family (Complex from Sulfolobus islandicus REY15A)
- Crispr-associated protein cmrx (Complex from Sulfolobus islandicus REY15A)
- Cmr1,crispr-associated ramp protein, cmr1 family (Complex from Sulfolobus islandicus REY15A)
- Manganese (ii) ion (54 Da, Ligand)
- Crrna (49-mer) (Complex from Sulfolobus islandicus REY15A)
- Cognate target rna (43-mer) (Complex from synthetic construct)
- Crispr-associated protein cmrx (19 kDa, Protein from Sulfolobus islandicus REY15A)
- Crrna (49-mer) (16 kDa, RNA from Sulfolobus islandicus REY15A)
- Cognate target rna (43-mer) (14 kDa, RNA from synthetic construct)
- Type iii-b cmr-beta ternary complex, cognate target rna and amppnp bound, in the presence of ssdna (900 kDa, Complex)
- Crispr-associated protein, cmr5 family (Complex from Sulfolobus islandicus REY15A)
- Crispr-associated ramp protein, cmr6 family (33 kDa, Protein from Sulfolobus islandicus REY15A)
- Crispr-associated protein, cmr3 family (36 kDa, Protein from Sulfolobus islandicus REY15A)
- Crispr-associated protein, cmr2 family (120 kDa, Protein from Sulfolobus islandicus REY15A)
- Crispr-associated protein, cmr5 family (17 kDa, Protein from Sulfolobus islandicus REY15A)
- Zinc ion (65 Da, Ligand)
- Crispr-associated ramp protein, cmr6 family (Complex from Sulfolobus islandicus REY15A)
- Crispr-associated ramp protein, cmr4 family (Complex from Sulfolobus islandicus REY15A)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
Cryo-EM structure of the Type III-B Cmr-beta bound to cognate target RNA
Resolution: 3.52 Å
EM Method: Single-particle
Fitted PDBs: 6sic
Q-score: 0.343
Sofos N, Feng M, Stella S, Pape T, Fuglsang A, Lin J, Huang Q, Li Y, She Q, Montoya G
Mol Cell (2020) 79 pp. 741-757.e7 [ DOI: doi:10.1016/j.molcel.2020.07.008 Pubmed: 32730741 ]
- Crispr-associated ramp protein, cmr1 family (55 kDa, Protein from Sulfolobus islandicus REY15A)
- Cognate target rna (14 kDa, RNA from Sulfolobus islandicus REY15A)
- Crispr-associated protein, cmr3 family (36 kDa, Protein from Sulfolobus islandicus REY15A)
- Crispr-associated ramp protein, cmr6 family (33 kDa, Protein from Sulfolobus islandicus REY15A)
- Zinc ion (65 Da, Ligand)
- Crispr-associated ramp protein, cmr4 family (32 kDa, Protein from Sulfolobus islandicus REY15A)
- Type iii-b cmr-beta binary complex, crrna-bound (Complex from Sulfolobus islandicus REY15A)
- Crispr-associated protein cmrx (19 kDa, Protein from Sulfolobus islandicus REY15A)
- Crrna (16 kDa, RNA from Sulfolobus islandicus REY15A)
- Crispr-associated protein, cmr2 family (120 kDa, Protein from Sulfolobus islandicus REY15A)
- Crispr-associated protein, cmr5 family (17 kDa, Protein from Sulfolobus islandicus REY15A)
- Type iii-b cmr-beta binary complex, crrna-bound (900 kDa, Complex)
Structures of the CRISPR-Cmr complex reveal mode of RNA target positioning
Taylor DW, Zhu Y, Staals RH, Kornfeld JE, Shinkai A, van der Oost J, Nogales E, Doudna JA
Science (2015) 348 pp. 581-585 [ Pubmed: 25837515 DOI: doi:10.1126/science.aaa4535 ]
- Apo crispr-cmr complex (365 kDa, Sample)
- Crispr rna (RNA from Thermus thermophilus)
- Crispr-associated protein tm1791 (38 kDa, Protein from Thermus thermophilus)
- Crispr-associated protein tm1795 (44 kDa, Protein from Thermus thermophilus)
- Crispr-associated protein cmr3 (39 kDa, Protein from Thermus thermophilus)
- Crispr-associated ramp cmr4 (37 kDa, Protein from Thermus thermophilus)
- Crispr-associated protein crm2 (65 kDa, Protein from Thermus thermophilus)
- Crispr system cmr subunit cmr5 (12 kDa, Protein from Thermus thermophilus)
Structures of the CRISPR-Cmr complex reveal mode of RNA target positioning
Taylor DW, Zhu Y, Staals RH, Kornfeld JE, Shinkai A, van der Oost J, Nogales E, Doudna JA
Science (2015) 348 pp. 581-585 [ Pubmed: 25837515 DOI: doi:10.1126/science.aaa4535 ]
- Crispr rna (RNA from Thermus thermophilus)
- Crispr-associated protein tm1791 (38 kDa, Protein from Thermus thermophilus)
- Smaller apo crispr-cmr complex (316 kDa, Sample)
- Crispr-associated protein tm1795 (44 kDa, Protein from Thermus thermophilus)
- Crispr-associated protein cmr3 (39 kDa, Protein from Thermus thermophilus)
- Crispr-associated ramp cmr4 (37 kDa, Protein from Thermus thermophilus)
- Crispr-associated protein crm2 (65 kDa, Protein from Thermus thermophilus)
- Crispr system cmr subunit cmr5 (12 kDa, Protein from Thermus thermophilus)
Structures of the CRISPR-Cmr complex reveal mode of RNA target positioning
Taylor DW, Zhu Y, Staals RH, Kornfeld JE, Shinkai A, van der Oost J, Nogales E, Doudna JA
Science (2015) 348 pp. 581-585 [ Pubmed: 25837515 DOI: doi:10.1126/science.aaa4535 ]
- Target-bound crispr-cmr complex (381 kDa, Sample)
- Crispr rna (RNA from Thermus thermophilus)
- Crispr-associated protein tm1791 (38 kDa, Protein from Thermus thermophilus)
- Crispr-associated protein tm1795 (44 kDa, Protein from Thermus thermophilus)
- Crispr-associated protein cmr3 (39 kDa, Protein from Thermus thermophilus)
- Crispr-associated ramp cmr4 (37 kDa, Protein from Thermus thermophilus)
- Crispr system cmr subunit cmr5 (12 kDa, Protein from Thermus thermophilus)
- Crispr-associated protein crm2 (65 kDa, Protein from Thermus thermophilus)
- Target rna (16 kDa, RNA from Thermus thermophilus)