A 3.7-angstrom structure of Yarrowia lipolytica complex I with an R121M mutation in NUCM.
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 7b0n
Q-score: 0.467
Hameedi MA, Grba DN, Richardson KH, Jones AJY, Song W, Roessler MM, Wright JJ, Hirst J
J Biol Chem (2021) 296 pp. 100474-100474 [ Pubmed: 33640456 DOI: doi:10.1016/j.jbc.2021.100474 ]
- Subunit nuam of nadh:ubiquinone oxidoreductase (complex i) (79 kDa, Protein from Yarrowia lipolytica)
- Subunit nipm of nadh:ubiquinone oxidoreductase (complex i) (10 kDa, Protein from Yarrowia lipolytica)
- Nucm protein (52 kDa, Protein from Yarrowia lipolytica)
- Subunit nunm of protein nadh:ubiquinone oxidoreductase (complex i) (15 kDa, Protein from Yarrowia lipolytica)
- Subunit nukm of nadh:ubiquinone oxidoreductase (complex i) (23 kDa, Protein from Yarrowia lipolytica)
- Grim-19 (13 kDa, Protein from Yarrowia lipolytica)
- Subunit ni8m of nadh:ubiquinone oxidoreductase (complex i) (9 kDa, Protein from Yarrowia lipolytica)
- Cardiolipin (1 kDa, Ligand)
- Nadh-ubiquinone oxidoreductase chain 4 (54 kDa, Protein from Yarrowia lipolytica)
- Subunit nb4m of protein nadh:ubiquinone oxidoreductase (complex i) (14 kDa, Protein from Yarrowia lipolytica)
- Subunit nuvm of nadh:ubiquinone oxidoreductase (complex i) (7 kDa, Protein from Yarrowia lipolytica)
- Yarrowia lipolytica mitochondrial complex i (nadh:ubiquinone oxidoreductase) with nucm r121m mutation (Complex from Yarrowia lipolytica)
- ~{s}-[2-[3-[[(2~{r})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{s})-3-oxidanyltetradecanethioate (584 Da, Ligand)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Acyl carrier protein (8 kDa, Protein from Yarrowia lipolytica)
- Subunit nebm of protein nadh:ubiquinone oxidoreductase (complex i) [yarrowia lipolytica] (7 kDa, Protein from Yarrowia lipolytica)
- Subunit nesm of nadh:ubiquinone oxidoreductase (complex i) (28 kDa, Protein from Yarrowia lipolytica)
- Flavin mononucleotide (456 Da, Ligand)
- Dodecyl-beta-d-maltoside (510 Da, Ligand)
- Nadh dehydrogenase subunit 2 (53 kDa, Protein from Yarrowia lipolytica)
- Nadh-ubiquinone oxidoreductase chain 5 (73 kDa, Protein from Yarrowia lipolytica)
- Subunit niam of nadh:ubiquinone oxidoreductase (complex i) (17 kDa, Protein from Yarrowia lipolytica)
- Subunit nupm of nadh:ubiquinone oxidoreductase (complex i) (19 kDa, Protein from Yarrowia lipolytica)
- 1,2-distearoyl-sn-glycerophosphoethanolamine (748 Da, Ligand)
- Nadh-ubiquinone oxidoreductase chain 1 (38 kDa, Protein from Yarrowia lipolytica)
- Zf-chcc domain-containing protein (13 kDa, Protein from Yarrowia lipolytica)
- Subunit nufm of nadh:ubiquinone oxidoreductase (complex i) (16 kDa, Protein from Yarrowia lipolytica)
- Subunit nb8m of nadh:ubiquinone oxidoreductase (complex i) (11 kDa, Protein from Yarrowia lipolytica)
- Subunit nb5m of nadh:ubiquinone oxidoreductase (complex i) (10 kDa, Protein from Yarrowia lipolytica)
- Subunit nuhm of nadh:ubiquinone oxidoreductase (complex i) (27 kDa, Protein from Yarrowia lipolytica)
- Nadh-ubiquinone oxidoreductase chain 4l (9 kDa, Protein from Yarrowia lipolytica)
- Subunit n7bm of nadh:ubiquinone oxidoreductase (complex i) (16 kDa, Protein from Yarrowia lipolytica)
- Subunit nuxm of nadh:ubiquinone oxidoreductase (complex i) (18 kDa, Protein from Yarrowia lipolytica)
- Subunit ni2m of nadh:ubiquinone oxidoreductase (complex i) (12 kDa, Protein from Yarrowia lipolytica)
- Acyl carrier protein (9 kDa, Protein from Yarrowia lipolytica)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 (9 kDa, Protein from Yarrowia lipolytica)
- Subunit ni9m of protein nadh:ubiquinone oxidoreductase (complex i) (8 kDa, Protein from Yarrowia lipolytica)
- Subunit nubm of nadh:ubiquinone oxidoreductase (complex i) (53 kDa, Protein from Yarrowia lipolytica)
- Zinc ion (65 Da, Ligand)
- Subunit nidm of nadh:ubiquinone oxidoreductase (complex i) (11 kDa, Protein from Yarrowia lipolytica)
- Nadph dihydro-nicotinamide-adenine-dinucleotide phosphate (745 Da, Ligand)
- Subunit nuym of nadh:ubiquinone oxidoreductase (complex i) (18 kDa, Protein from Yarrowia lipolytica)
- Subunit nuum of nadh:ubiquinone oxidoreductase (complex i) (9 kDa, Protein from Yarrowia lipolytica)
- Nadh-ubiquinone oxidoreductase chain 6 (20 kDa, Protein from Yarrowia lipolytica)
- Subunit nb2m of nadh:ubiquinone oxidoreductase (complex i) (6 kDa, Protein from Yarrowia lipolytica)
- Diundecyl phosphatidyl choline (622 Da, Ligand)
- Subunit nuzm of nadh:ubiquinone oxidoreductase (complex i) (19 kDa, Protein from Yarrowia lipolytica)
- Nugm protein (33 kDa, Protein from Yarrowia lipolytica)
- Nadh-ubiquinone oxidoreductase chain 3 (14 kDa, Protein from Yarrowia lipolytica)
- Iron/sulfur cluster (351 Da, Ligand)
- Epimerase domain-containing protein (40 kDa, Protein from Yarrowia lipolytica)
- Subunit nuim of protein nadh:ubiquinone oxidoreductase (complex i) (21 kDa, Protein from Yarrowia lipolytica)
- Complex i-b14.7 (18 kDa, Protein from Yarrowia lipolytica)
Entire ovine respiratory complex I, Combined Map
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 5lnk
Q-score: 0.408
Fiedorczuk K, Letts JA, Degliesposti G, Kaszuba K, Skehel M, Sazanov LA
Nature (2016) 538 pp. 406-410 [ Pubmed: 27595392 DOI: doi:10.1038/nature19794 ]
- Mitochondrial complex i, 49 kda subunit (47 kDa, Protein from Sheep)
- Cardiolipin (1 kDa, Ligand)
- Mitochondrial complex i, b15 subunit (14 kDa, Protein from Sheep)
- Mitochondrial complex i, 24 kda subunit (23 kDa, Protein from Sheep)
- Mitochondrial complex i, b22 subunit (21 kDa, Protein from Sheep)
- Mitochondrial complex i, kfyi subunit (5 kDa, Protein from Sheep)
- Mitochondrial complex i (970 kDa, Complex from Ovis aries)
- Mitochondrial complex i, nd1 subunit (35 kDa, Protein from Sheep)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Flavin mononucleotide (456 Da, Ligand)
- 1,2-diacyl-sn-glycero-3-phosphoethanolamine (748 Da, Ligand)
- 4'-phosphopantetheine (358 Da, Ligand)
- Mitochondrial complex i, b14.7 subunit (10 kDa, Protein from Sheep)
- Mitochondrial complex i, b16.6 subunit (16 kDa, Protein from Sheep)
- Mitochondrial complex i, aggg subunit (8 kDa, Protein from Sheep)
- Mitochondrial complex i, nd4 subunit (52 kDa, Protein from Sheep)
- Mitochondrial complex i, 39 kda subunit (38 kDa, Protein from Sheep)
- Mitochondrial complex i, nd2 subunit (39 kDa, Protein from Sheep)
- Mitochondrial complex i, nd4l subunit (10 kDa, Protein from Sheep)
- Mitochondrial complex i, 18 kda subunit (15 kDa, Protein from Sheep)
- Acyl carrier protein (10 kDa, Protein from Sheep)
- Mitochondrial complex i, 15 kda subunit (12 kDa, Protein from Sheep)
- Mitochondrial complex i, b14.5b subunit (14 kDa, Protein from Sheep)
- Mitochondrial complex i, pdsw subunit (20 kDa, Protein from Sheep)
- Mitochondrial complex i, 30 kda subunit (26 kDa, Protein from Sheep)
- Mitochondrial complex i, nd3 subunit (13 kDa, Protein from Sheep)
- Mitochondrial complex i, nd6 subunit (19 kDa, Protein from Sheep)
- Mitochondrial complex i, mwfe subunit (8 kDa, Protein from Sheep)
- Mitochondrial complex i, sgdh subunit (16 kDa, Protein from Sheep)
- Mitochondrial complex i, mnll subunit (6 kDa, Protein from Sheep)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial (18 kDa, Protein from Sheep)
- Mitochondrial complex i, esss subunit (14 kDa, Protein from Sheep)
- Mitochondrial complex i, b17.2 subunit (17 kDa, Protein from Sheep)
- Mitochondrial complex i, b8 subunit (10 kDa, Protein from Sheep)
- Mitochondrial complex i, b14 subunit (14 kDa, Protein from Sheep)
- Mitochondrial complex i, 51 kda subunit (48 kDa, Protein from Sheep)
- Mitochondrial complex i, b14.5a subunit (12 kDa, Protein from Sheep)
- Mitochondrial complex i, 10 kda subunit (8 kDa, Protein from Sheep)
- Mitochondrial complex i, b9 subunit (9 kDa, Protein from Sheep)
- Mitochondrial complex i, b17 subunit (15 kDa, Protein from Sheep)
- Zinc ion (65 Da, Ligand)
- Nadph dihydro-nicotinamide-adenine-dinucleotide phosphate (745 Da, Ligand)
- Mitochondrial complex i, b13 subunit (13 kDa, Protein from Sheep)
- Mitochondrial complex i, 13 kda subunit (10 kDa, Protein from Sheep)
- Mitochondrial complex i, 42 kda subunit (36 kDa, Protein from Sheep)
- Mitochondrial complex i, b18 subunit (16 kDa, Protein from Sheep)
- 1,2-diacyl-sn-glycero-3-phosphocholine (790 Da, Ligand)
- Mitochondrial complex i, 75 kda subunit (77 kDa, Protein from Sheep)
- Mitochondrial complex i, psst subunit (20 kDa, Protein from Sheep)
- Iron/sulfur cluster (351 Da, Ligand)
- Mitochondrial complex i, pgiv subunit (20 kDa, Protein from Sheep)
- Mitochondrial complex i, tyky subunit (20 kDa, Protein from Sheep)
- Mitochondrial complex i, nd5 subunit (67 kDa, Protein from Sheep)
- Mitochondrial complex i, b12 subunit (11 kDa, Protein from Sheep)
- S-[2-({n-[(2s)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate (568 Da, Ligand)