In-situ structure of typeA supercomplex with lipids in respiratory chain (composite)
Resolution: 2.1 Å
EM Method: Single-particle
Fitted PDBs: 8ugh
Q-score: 0.464
Zheng W, Chai P, Zhu J, Zhang K
Nature (2024) 631 pp. 232-239 [ Pubmed: 38811722 DOI: doi:10.1038/s41586-024-07488-9 ]
- Cytochrome c1 (35 kDa, Protein from Sus scrofa)
- Ubiquinol-cytochrome c reductase complex 7.2 kda protein (7 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial (50 kDa, Protein from Sus scrofa)
- Nadh-ubiquinone oxidoreductase chain 3 (13 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 isoform x1 (13 kDa, Protein from Sus scrofa)
- Cytochrome b-c1 complex subunit 1, mitochondrial (52 kDa, Protein from Sus scrofa)
- Nadh-ubiquinone oxidoreductase chain 4l (10 kDa, Protein from Sus scrofa)
- Zinc ion (65 Da, Ligand)
- Cytochrome c oxidase subunit 2 (26 kDa, Protein from Sus scrofa)
- Cytochrome b-c1 complex subunit 8 (9 kDa, Protein from Sus scrofa)
- Heme-a (852 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial (17 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 (9 kDa, Protein from Sus scrofa)
- Cytochrome c oxidase subunit 1 (57 kDa, Protein from Sus scrofa)
- Iron/sulfur cluster (351 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 (20 kDa, Protein from Sus scrofa)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial (27 kDa, Protein from Sus scrofa)
- Cytochrome c oxidase subunit 3 (29 kDa, Protein from Sus scrofa)
- ~{s}-[2-[3-[[(2~{r})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{s})-3-oxidanyltetradecanethioate (584 Da, Ligand)
- Cytochrome b-c1 complex subunit 6, mitochondrial (10 kDa, Protein from Sus scrofa)
- Nadph dihydro-nicotinamide-adenine-dinucleotide phosphate (745 Da, Ligand)
- (1s)-2-{[(2-aminoethoxy)(hydroxy)phosphoryl]oxy}-1-[(stearoyloxy)methyl]ethyl (5e,8e,11e,14e)-icosa-5,8,11,14-tetraenoate (768 Da, Ligand)
- N-acetylalanine (131 Da, Ligand)
- Cytochrome c oxidase subunit ndufa4 (9 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial (21 kDa, Protein from Sus scrofa)
- Cytochrome b-c1 complex subunit 10 (6 kDa, Protein from Sus scrofa)
- 1,2-diacyl-sn-glycero-3-phosphocholine (790 Da, Ligand)
- Cytochrome c oxidase subunit 4 (19 kDa, Protein from Sus scrofa)
- Cytochrome c oxidase subunit 6c (8 kDa, Protein from Sus scrofa)
- Protoporphyrin ix containing fe (616 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial (40 kDa, Protein from Sus scrofa)
- Sodium ion (22 Da, Ligand)
- Copper (ii) ion (63 Da, Ligand)
- Cytochrome b-c1 complex subunit rieske, mitochondrial (29 kDa, Protein from Sus scrofa)
- Cytochrome c oxidase subunit 8 (7 kDa, Protein from Sus scrofa)
- In-situ cryo-em structure of respiratory supercomplex by directly imaging mitochondria (Complex from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial (27 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 [sus scrofa] (15 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 (12 kDa, Protein from Sus scrofa)
- Cytochrome c oxidase subunit 6b1 (10 kDa, Protein from Sus scrofa)
- Nadh-ubiquinone oxidoreductase chain 1 (35 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 subunit c1, mitochondrial (8 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 (8 kDa, Protein from Sus scrofa)
- (7r,17e,20e)-4-hydroxy-n,n,n-trimethyl-9-oxo-7-[(palmitoyloxy)methyl]-3,5,8-trioxa-4-phosphahexacosa-17,20-dien-1-aminium 4-oxide (759 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 (20 kDa, Protein from Sus scrofa)
- 1,2-distearoyl-sn-glycerophosphoethanolamine (748 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 (15 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial (52 kDa, Protein from Sus scrofa)
- Myristic acid (228 Da, Ligand)
- (1r)-2-{[{[(2s)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(palmitoyloxy)methyl]ethyl (11e)-octadec-11-enoate (749 Da, Ligand)
- Nadh-ubiquinone oxidoreductase chain 4 (51 kDa, Protein from Sus scrofa)
- Phosphate ion (94 Da, Ligand)
- Dinuclear copper ion (127 Da, Ligand)
- Cytochrome b (42 kDa, Protein from Sus scrofa)
- Cytochrome c oxidase subunit 6a2 (10 kDa, Protein from Sus scrofa)
- Cytochrome c oxidase subunit 7c, mitochondrial (7 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 5 (12 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial (21 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial (48 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial (13 kDa, Protein from Sus scrofa)
- Nadh:ubiquinone oxidoreductase subunit a9 (42 kDa, Protein from Sus scrofa)
- Potassium ion (39 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 (14 kDa, Protein from Sus scrofa)
- Cytochrome c oxidase subunit 5b, mitochondrial (13 kDa, Protein from Sus scrofa)
- Heme c (618 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial (30 kDa, Protein from Sus scrofa)
- Nadh:ubiquinone oxidoreductase subunit ab1 (17 kDa, Protein from Sus scrofa)
- Flavin mononucleotide (456 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 (16 kDa, Protein from Sus scrofa)
- Nadh-ubiquinone oxidoreductase chain 6 (19 kDa, Protein from Sus scrofa)
- Nadh:ubiquinone oxidoreductase subunit b2 (11 kDa, Protein from Sus scrofa)
- Cytochrome c oxidase subunit 7a1, mitochondrial (9 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 (11 kDa, Protein from Sus scrofa)
- Nadh:ubiquinone oxidoreductase subunit a13 (16 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 subunit c2 (14 kDa, Protein from Sus scrofa)
- Cytochrome c oxidase subunit 5a, mitochondrial (16 kDa, Protein from Sus scrofa)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 (14 kDa, Protein from Sus scrofa)
- Cardiolipin (1 kDa, Ligand)
- Nadh-ubiquinone oxidoreductase chain 2 (39 kDa, Protein from Sus scrofa)
- (1s)-2-{[{[(2r)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(palmitoyloxy)methyl]ethyl stearate (751 Da, Ligand)
- Cytochrome c oxidase subunit 7b (9 kDa, Protein from Sus scrofa)
- Magnesium ion (24 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial (19 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 (21 kDa, Protein from Sus scrofa)
- Nadh-ubiquinone oxidoreductase chain 5 (68 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 (17 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 (11 kDa, Protein from Sus scrofa)
- Nadh-ubiquinone oxidoreductase 75 kda subunit, mitochondrial (79 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial (28 kDa, Protein from Sus scrofa)
- Cytochrome b-c1 complex subunit 7 (13 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 (15 kDa, Protein from Sus scrofa)
- Cytochrome b-c1 complex subunit 2, mitochondrial (48 kDa, Protein from Sus scrofa)
- N-acetylmethionine (191 Da, Ligand)
High resolution in-situ structure of typeA supercomplex in respiratory chain (I1III2IV1,composite)
Resolution: 2.1 Å
EM Method: Single-particle
Fitted PDBs: 8ugi
Q-score: 0.629
Zheng W, Chai P, Zhu J, Zhang K
Nature (2024) 631 pp. 232-239 [ DOI: doi:10.1038/s41586-024-07488-9 Pubmed: 38811722 ]
- Cytochrome b-c1 complex subunit 10 (6 kDa, Protein from Sus scrofa)
- N-acetylmethionine (191 Da, Ligand)
- Cytochrome c oxidase subunit 8 (7 kDa, Protein from Sus scrofa)
- Nadh:ubiquinone oxidoreductase subunit a13 (16 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 (21 kDa, Protein from Sus scrofa)
- Cytochrome b (42 kDa, Protein from Sus scrofa)
- Protoporphyrin ix containing fe (616 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 subunit c2 (14 kDa, Protein from Sus scrofa)
- Heme c (618 Da, Ligand)
- Nadh:ubiquinone oxidoreductase subunit ab1 (17 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial (30 kDa, Protein from Sus scrofa)
- Nadh:ubiquinone oxidoreductase subunit b2 (11 kDa, Protein from Sus scrofa)
- Phosphate ion (94 Da, Ligand)
- Cardiolipin (1 kDa, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 (20 kDa, Protein from Sus scrofa)
- Nadh-ubiquinone oxidoreductase chain 2 (39 kDa, Protein from Sus scrofa)
- Cytochrome b-c1 complex subunit 6, mitochondrial (10 kDa, Protein from Sus scrofa)
- Copper (ii) ion (63 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 (14 kDa, Protein from Sus scrofa)
- Cytochrome c oxidase subunit 7a1, mitochondrial (9 kDa, Protein from Sus scrofa)
- Nadh-ubiquinone oxidoreductase chain 1 (35 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial (21 kDa, Protein from Sus scrofa)
- Cytochrome b-c1 complex subunit 1, mitochondrial (52 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial (27 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 isoform x1 (13 kDa, Protein from Sus scrofa)
- Sodium ion (22 Da, Ligand)
- Cytochrome c oxidase subunit 2 (26 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial (13 kDa, Protein from Sus scrofa)
- (1s)-2-{[(2-aminoethoxy)(hydroxy)phosphoryl]oxy}-1-[(stearoyloxy)methyl]ethyl (5e,8e,11e,14e)-icosa-5,8,11,14-tetraenoate (768 Da, Ligand)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Cytochrome c oxidase subunit 5a, mitochondrial (16 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 (17 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 (11 kDa, Protein from Sus scrofa)
- Nadh:ubiquinone oxidoreductase subunit a9 (42 kDa, Protein from Sus scrofa)
- Cytochrome c oxidase subunit 5b, mitochondrial (13 kDa, Protein from Sus scrofa)
- Magnesium ion (24 Da, Ligand)
- Cytochrome b-c1 complex subunit rieske, mitochondrial (29 kDa, Protein from Sus scrofa)
- Cytochrome c oxidase subunit 7c, mitochondrial (7 kDa, Protein from Sus scrofa)
- Iron/sulfur cluster (351 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial (27 kDa, Protein from Sus scrofa)
- Cytochrome c oxidase subunit 7b (9 kDa, Protein from Sus scrofa)
- Ubiquinol-cytochrome c reductase complex 7.2 kda protein (7 kDa, Protein from Sus scrofa)
- Flavin mononucleotide (456 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial (48 kDa, Protein from Sus scrofa)
- Cytochrome b-c1 complex subunit 2, mitochondrial (48 kDa, Protein from Sus scrofa)
- Cytochrome c oxidase subunit 1 (56 kDa, Protein from Sus scrofa)
- Nadh-ubiquinone oxidoreductase chain 4l (10 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 (14 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 (15 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial (19 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 (20 kDa, Protein from Sus scrofa)
- 1,2-distearoyl-sn-glycerophosphoethanolamine (748 Da, Ligand)
- ~{s}-[2-[3-[[(2~{r})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{s})-3-oxidanyltetradecanethioate (584 Da, Ligand)
- Dinuclear copper ion (127 Da, Ligand)
- Nadh-ubiquinone oxidoreductase chain 6 (19 kDa, Protein from Sus scrofa)
- Cytochrome c oxidase subunit ndufa4 (9 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 subunit c1, mitochondrial (8 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 (16 kDa, Protein from Sus scrofa)
- Nadh-ubiquinone oxidoreductase chain 3 (13 kDa, Protein from Sus scrofa)
- Nadh-ubiquinone oxidoreductase 75 kda subunit, mitochondrial (79 kDa, Protein from Sus scrofa)
- (1r)-2-{[{[(2s)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(palmitoyloxy)methyl]ethyl (11e)-octadec-11-enoate (749 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 5 (12 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 (8 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial (50 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 (9 kDa, Protein from Sus scrofa)
- Cytochrome c oxidase subunit 4 (19 kDa, Protein from Sus scrofa)
- Cytochrome c1, heme protein, mitochondrial (35 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 (15 kDa, Protein from Sus scrofa)
- (7r,17e,20e)-4-hydroxy-n,n,n-trimethyl-9-oxo-7-[(palmitoyloxy)methyl]-3,5,8-trioxa-4-phosphahexacosa-17,20-dien-1-aminium 4-oxide (759 Da, Ligand)
- Potassium ion (39 Da, Ligand)
- Cytochrome b-c1 complex subunit 8 (9 kDa, Protein from Sus scrofa)
- Zinc ion (65 Da, Ligand)
- Nadh-ubiquinone oxidoreductase chain 5 (68 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial (17 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial (21 kDa, Protein from Sus scrofa)
- Water (18 Da, Ligand)
- 1,2-diacyl-sn-glycero-3-phosphocholine (790 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial (54 kDa, Protein from Sus scrofa)
- Nadh-ubiquinone oxidoreductase chain 4 (51 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial (28 kDa, Protein from Sus scrofa)
- Cytochrome c oxidase subunit 6b1 (10 kDa, Protein from Sus scrofa)
- In-situ cryo-em structure of respiratory supercomplex by directly imaging mitochondria (Complex from Sus scrofa)
- Nadph dihydro-nicotinamide-adenine-dinucleotide phosphate (745 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 (12 kDa, Protein from Sus scrofa)
- Heme-a (852 Da, Ligand)
- Cytochrome c oxidase subunit 6c (8 kDa, Protein from Sus scrofa)
- Cytochrome c oxidase subunit 3 (29 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial (40 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 [sus scrofa] (15 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 (11 kDa, Protein from Sus scrofa)
- Cytochrome c oxidase subunit 6a2 (10 kDa, Protein from Sus scrofa)
- Myristic acid (228 Da, Ligand)
- Cytochrome b-c1 complex subunit 7 (13 kDa, Protein from Sus scrofa)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
In-situ structure of typeB supercomplex in respiratory chain (composite)
Resolution: 2.3 Å
EM Method: Single-particle
Fitted PDBs: 8ugj
Q-score: 0.495
Zheng W, Chai P, Zhu J, Zhang K
Nature (2024) 631 pp. 232-239 [ DOI: doi:10.1038/s41586-024-07488-9 Pubmed: 38811722 ]
- Cytochrome b-c1 complex subunit 10 (6 kDa, Protein from Sus scrofa)
- N-acetylmethionine (191 Da, Ligand)
- Cytochrome c oxidase subunit 8 (7 kDa, Protein from Sus scrofa)
- Nadh:ubiquinone oxidoreductase subunit a13 (16 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 (21 kDa, Protein from Sus scrofa)
- Cytochrome b (42 kDa, Protein from Sus scrofa)
- Protoporphyrin ix containing fe (616 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 subunit c2 (14 kDa, Protein from Sus scrofa)
- Heme c (618 Da, Ligand)
- Nadh:ubiquinone oxidoreductase subunit ab1 (17 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial (30 kDa, Protein from Sus scrofa)
- Nadh:ubiquinone oxidoreductase subunit b2 (11 kDa, Protein from Sus scrofa)
- Phosphate ion (94 Da, Ligand)
- Cardiolipin (1 kDa, Ligand)
- Cytochrome b-c1 complex subunit rieske, mitochondrial (29 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 (20 kDa, Protein from Sus scrofa)
- Nadh-ubiquinone oxidoreductase chain 2 (39 kDa, Protein from Sus scrofa)
- Cytochrome b-c1 complex subunit 6, mitochondrial (10 kDa, Protein from Sus scrofa)
- N-acetylalanine (131 Da, Ligand)
- Copper (ii) ion (63 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 (14 kDa, Protein from Sus scrofa)
- Cytochrome c oxidase subunit 7a1, mitochondrial (9 kDa, Protein from Sus scrofa)
- Nadh-ubiquinone oxidoreductase chain 1 (35 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial (21 kDa, Protein from Sus scrofa)
- Cytochrome b-c1 complex subunit 1, mitochondrial (52 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial (27 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 isoform x1 (13 kDa, Protein from Sus scrofa)
- Sodium ion (22 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial (13 kDa, Protein from Sus scrofa)
- (1s)-2-{[(2-aminoethoxy)(hydroxy)phosphoryl]oxy}-1-[(stearoyloxy)methyl]ethyl (5e,8e,11e,14e)-icosa-5,8,11,14-tetraenoate (768 Da, Ligand)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Cytochrome c oxidase subunit 5a, mitochondrial (16 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 (17 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 (11 kDa, Protein from Sus scrofa)
- Nadh:ubiquinone oxidoreductase subunit a9 (42 kDa, Protein from Sus scrofa)
- Cytochrome c oxidase subunit 5b, mitochondrial (13 kDa, Protein from Sus scrofa)
- Magnesium ion (24 Da, Ligand)
- Cytochrome c oxidase subunit 7c, mitochondrial (7 kDa, Protein from Sus scrofa)
- Iron/sulfur cluster (351 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial (27 kDa, Protein from Sus scrofa)
- Cytochrome c oxidase subunit 7b (9 kDa, Protein from Sus scrofa)
- Flavin mononucleotide (456 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial (48 kDa, Protein from Sus scrofa)
- Cytochrome b-c1 complex subunit 2, mitochondrial (48 kDa, Protein from Sus scrofa)
- Cytochrome c oxidase subunit 1 (57 kDa, Protein from Sus scrofa)
- Nadh-ubiquinone oxidoreductase chain 4l (10 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 (14 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 (15 kDa, Protein from Sus scrofa)
- Cytochrome c oxidase subunit 2 (26 kDa, Protein from Sus scrofa)
- Cholic acid (408 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial (19 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 (20 kDa, Protein from Sus scrofa)
- 1,2-distearoyl-sn-glycerophosphoethanolamine (748 Da, Ligand)
- ~{s}-[2-[3-[[(2~{r})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{s})-3-oxidanyltetradecanethioate (584 Da, Ligand)
- Dinuclear copper ion (127 Da, Ligand)
- Nadh-ubiquinone oxidoreductase chain 6 (19 kDa, Protein from Sus scrofa)
- Cytochrome c oxidase subunit ndufa4 (9 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 subunit c1, mitochondrial (8 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 (16 kDa, Protein from Sus scrofa)
- Nadh-ubiquinone oxidoreductase chain 3 (13 kDa, Protein from Sus scrofa)
- Nadh-ubiquinone oxidoreductase 75 kda subunit, mitochondrial (79 kDa, Protein from Sus scrofa)
- (1r)-2-{[{[(2s)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(palmitoyloxy)methyl]ethyl (11e)-octadec-11-enoate (749 Da, Ligand)
- Cytochrome c1 (35 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 5 (12 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 (8 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial (50 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 (9 kDa, Protein from Sus scrofa)
- Cytochrome c oxidase subunit 4 (19 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 (15 kDa, Protein from Sus scrofa)
- (7r,17e,20e)-4-hydroxy-n,n,n-trimethyl-9-oxo-7-[(palmitoyloxy)methyl]-3,5,8-trioxa-4-phosphahexacosa-17,20-dien-1-aminium 4-oxide (759 Da, Ligand)
- Potassium ion (39 Da, Ligand)
- Cytochrome b-c1 complex subunit 8 (9 kDa, Protein from Sus scrofa)
- Zinc ion (65 Da, Ligand)
- Nadh-ubiquinone oxidoreductase chain 5 (68 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial (17 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial (21 kDa, Protein from Sus scrofa)
- 1,2-diacyl-sn-glycero-3-phosphocholine (790 Da, Ligand)
- Complex iii subunit 9 (6 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial (54 kDa, Protein from Sus scrofa)
- Nadh-ubiquinone oxidoreductase chain 4 (51 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial (28 kDa, Protein from Sus scrofa)
- Cytochrome c oxidase subunit 6b1 (10 kDa, Protein from Sus scrofa)
- In-situ cryo-em structure of respiratory supercomplex by directly imaging mitochondria (Complex from Sus scrofa)
- Nadph dihydro-nicotinamide-adenine-dinucleotide phosphate (745 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 (12 kDa, Protein from Sus scrofa)
- Heme-a (852 Da, Ligand)
- Cytochrome c oxidase subunit 6c (8 kDa, Protein from Sus scrofa)
- Cytochrome c oxidase subunit 3 (29 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial (40 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 [sus scrofa] (15 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 (11 kDa, Protein from Sus scrofa)
- Cytochrome c oxidase subunit 6a2 (10 kDa, Protein from Sus scrofa)
- Myristic acid (228 Da, Ligand)
- Cytochrome b-c1 complex subunit 7 (13 kDa, Protein from Sus scrofa)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
High resolution in-situ structure of complex IV in respiratory supercomplex
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 8ugl
Q-score: 0.592
Zheng W, Chai P, Zhu J, Zhang K
Nature (2024) 631 pp. 232-239 [ DOI: doi:10.1038/s41586-024-07488-9 Pubmed: 38811722 ]
- Cytochrome c oxidase subunit 1 (56 kDa, Protein from Sus scrofa)
- Cytochrome c oxidase subunit 8 (7 kDa, Protein from Sus scrofa)
- Cytochrome c oxidase subunit 2 (26 kDa, Protein from Sus scrofa)
- Zinc ion (65 Da, Ligand)
- Sodium ion (22 Da, Ligand)
- Water (18 Da, Ligand)
- Dinuclear copper ion (127 Da, Ligand)
- (1s)-2-{[(2-aminoethoxy)(hydroxy)phosphoryl]oxy}-1-[(stearoyloxy)methyl]ethyl (5e,8e,11e,14e)-icosa-5,8,11,14-tetraenoate (768 Da, Ligand)
- Cytochrome c oxidase subunit ndufa4 (9 kDa, Protein from Sus scrofa)
- Cytochrome c oxidase subunit 5a, mitochondrial (16 kDa, Protein from Sus scrofa)
- Phosphate ion (94 Da, Ligand)
- Cardiolipin (1 kDa, Ligand)
- (7r,17e,20e)-4-hydroxy-n,n,n-trimethyl-9-oxo-7-[(palmitoyloxy)methyl]-3,5,8-trioxa-4-phosphahexacosa-17,20-dien-1-aminium 4-oxide (759 Da, Ligand)
- (1r)-2-{[{[(2s)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(palmitoyloxy)methyl]ethyl (11e)-octadec-11-enoate (749 Da, Ligand)
- Cytochrome c oxidase subunit 5b, mitochondrial (13 kDa, Protein from Sus scrofa)
- Copper (ii) ion (63 Da, Ligand)
- Cytochrome c oxidase subunit 6b1 (10 kDa, Protein from Sus scrofa)
- Cytochrome c oxidase subunit 7a1, mitochondrial (9 kDa, Protein from Sus scrofa)
- Heme-a (852 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Cytochrome c oxidase subunit 3 (29 kDa, Protein from Sus scrofa)
- Cytochrome c oxidase subunit 6c (8 kDa, Protein from Sus scrofa)
- Cytochrome c oxidase subunit 7c, mitochondrial (7 kDa, Protein from Sus scrofa)
- Cytochrome c oxidase subunit 4 (19 kDa, Protein from Sus scrofa)
- In-situ cryo-em structure of respiratory supercomplex by directly imaging mitochondria (Cellular component from Sus scrofa)
- Cytochrome c oxidase subunit 7b (9 kDa, Protein from Sus scrofa)
- Cytochrome c oxidase subunit 6a2 (10 kDa, Protein from Sus scrofa)
In-situ structure of typeO supercomplex in respiratory chain (composite)
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 8ugn
Q-score: 0.536
Zheng W, Chai P, Zhu J, Zhang K
Nature (2024) 631 pp. 232-239 [ DOI: doi:10.1038/s41586-024-07488-9 Pubmed: 38811722 ]
- Cytochrome b-c1 complex subunit 10 (6 kDa, Protein from Sus scrofa)
- N-acetylmethionine (191 Da, Ligand)
- Cytochrome c oxidase subunit 8 (7 kDa, Protein from Sus scrofa)
- Nadh:ubiquinone oxidoreductase subunit a13 (16 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 (21 kDa, Protein from Sus scrofa)
- Cytochrome b (42 kDa, Protein from Sus scrofa)
- Protoporphyrin ix containing fe (616 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 subunit c2 (14 kDa, Protein from Sus scrofa)
- Heme c (618 Da, Ligand)
- Ubiquinol-cytochrome c reductase complex 7.2 kda protein 53 complext iii (7 kDa, Protein from Sus scrofa)
- Nadh:ubiquinone oxidoreductase subunit ab1 (17 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial (30 kDa, Protein from Sus scrofa)
- Nadh:ubiquinone oxidoreductase subunit b2 (11 kDa, Protein from Sus scrofa)
- Phosphate ion (94 Da, Ligand)
- Cardiolipin (1 kDa, Ligand)
- Cytochrome b-c1 complex subunit rieske, mitochondrial (29 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 (20 kDa, Protein from Sus scrofa)
- Nadh-ubiquinone oxidoreductase chain 2 (39 kDa, Protein from Sus scrofa)
- Cytochrome b-c1 complex subunit 6, mitochondrial (10 kDa, Protein from Sus scrofa)
- N-acetylalanine (131 Da, Ligand)
- Copper (ii) ion (63 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 (14 kDa, Protein from Sus scrofa)
- Cytochrome c oxidase subunit 7a1, mitochondrial (9 kDa, Protein from Sus scrofa)
- Nadh-ubiquinone oxidoreductase chain 1 (35 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial (21 kDa, Protein from Sus scrofa)
- Cytochrome b-c1 complex subunit 1, mitochondrial (52 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial (27 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 isoform x1 (13 kDa, Protein from Sus scrofa)
- Sodium ion (22 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial (13 kDa, Protein from Sus scrofa)
- (1s)-2-{[(2-aminoethoxy)(hydroxy)phosphoryl]oxy}-1-[(stearoyloxy)methyl]ethyl (5e,8e,11e,14e)-icosa-5,8,11,14-tetraenoate (768 Da, Ligand)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Cytochrome c oxidase subunit 5a, mitochondrial (16 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 (17 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 (11 kDa, Protein from Sus scrofa)
- Nadh:ubiquinone oxidoreductase subunit a9 (42 kDa, Protein from Sus scrofa)
- Cytochrome c oxidase subunit 5b, mitochondrial (13 kDa, Protein from Sus scrofa)
- Magnesium ion (24 Da, Ligand)
- Cytochrome c oxidase subunit 7c, mitochondrial (7 kDa, Protein from Sus scrofa)
- Iron/sulfur cluster (351 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial (27 kDa, Protein from Sus scrofa)
- Cytochrome c oxidase subunit 7b (9 kDa, Protein from Sus scrofa)
- Flavin mononucleotide (456 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial (48 kDa, Protein from Sus scrofa)
- Cytochrome b-c1 complex subunit 2, mitochondrial (48 kDa, Protein from Sus scrofa)
- Cytochrome c oxidase subunit 1 (57 kDa, Protein from Sus scrofa)
- Nadh-ubiquinone oxidoreductase chain 4l (10 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 (14 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 (15 kDa, Protein from Sus scrofa)
- Cytochrome c oxidase subunit 2 (26 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial (19 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 (20 kDa, Protein from Sus scrofa)
- 1,2-distearoyl-sn-glycerophosphoethanolamine (748 Da, Ligand)
- ~{s}-[2-[3-[[(2~{r})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{s})-3-oxidanyltetradecanethioate (584 Da, Ligand)
- Dinuclear copper ion (127 Da, Ligand)
- Nadh-ubiquinone oxidoreductase chain 6 (19 kDa, Protein from Sus scrofa)
- Cytochrome c oxidase subunit ndufa4 (9 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 subunit c1, mitochondrial (8 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 (16 kDa, Protein from Sus scrofa)
- Nadh-ubiquinone oxidoreductase chain 3 (13 kDa, Protein from Sus scrofa)
- Nadh-ubiquinone oxidoreductase 75 kda subunit, mitochondrial (79 kDa, Protein from Sus scrofa)
- (1r)-2-{[{[(2s)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(palmitoyloxy)methyl]ethyl (11e)-octadec-11-enoate (749 Da, Ligand)
- Cytochrome c1 (35 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 5 (12 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 (15 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 (8 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial (50 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 (9 kDa, Protein from Sus scrofa)
- Cytochrome c oxidase subunit 4 (19 kDa, Protein from Sus scrofa)
- (7r,17e,20e)-4-hydroxy-n,n,n-trimethyl-9-oxo-7-[(palmitoyloxy)methyl]-3,5,8-trioxa-4-phosphahexacosa-17,20-dien-1-aminium 4-oxide (759 Da, Ligand)
- Potassium ion (39 Da, Ligand)
- Cytochrome b-c1 complex subunit 8 (9 kDa, Protein from Sus scrofa)
- Zinc ion (65 Da, Ligand)
- Nadh-ubiquinone oxidoreductase chain 5 (68 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial (17 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial (21 kDa, Protein from Sus scrofa)
- 1,2-diacyl-sn-glycero-3-phosphocholine (790 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial (54 kDa, Protein from Sus scrofa)
- Nadh-ubiquinone oxidoreductase chain 4 (51 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial (28 kDa, Protein from Sus scrofa)
- Cytochrome c oxidase subunit 6b1 (10 kDa, Protein from Sus scrofa)
- In-situ cryo-em structure of respiratory supercomplex by directly imaging mitochondria (Complex from Sus scrofa)
- Nadph dihydro-nicotinamide-adenine-dinucleotide phosphate (745 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 (12 kDa, Protein from Sus scrofa)
- Heme-a (852 Da, Ligand)
- Cytochrome c oxidase subunit 6c (8 kDa, Protein from Sus scrofa)
- Cytochrome c oxidase subunit 3 (29 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial (40 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 [sus scrofa] (15 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 (11 kDa, Protein from Sus scrofa)
- Cytochrome c oxidase subunit 6a2 (10 kDa, Protein from Sus scrofa)
- Myristic acid (228 Da, Ligand)
- Cytochrome b-c1 complex subunit 7 (13 kDa, Protein from Sus scrofa)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
In-situ structure of typeX supercomplex in respiratory chain (composite)
Resolution: 6.5 Å
EM Method: Single-particle
Fitted PDBs: 8ugr
Q-score: 0.164
Zheng W, Chai P, Zhu J, Zhang K
Nature (2024) 631 pp. 232-239 [ DOI: doi:10.1038/s41586-024-07488-9 Pubmed: 38811722 ]
- Cytochrome b-c1 complex subunit 10 (6 kDa, Protein from Sus scrofa)
- N-acetylmethionine (191 Da, Ligand)
- Cytochrome c oxidase subunit 8 (7 kDa, Protein from Sus scrofa)
- Nadh:ubiquinone oxidoreductase subunit a13 (16 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 (21 kDa, Protein from Sus scrofa)
- Cytochrome b (42 kDa, Protein from Sus scrofa)
- Protoporphyrin ix containing fe (616 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 subunit c2 (14 kDa, Protein from Sus scrofa)
- Heme c (618 Da, Ligand)
- Nadh:ubiquinone oxidoreductase subunit ab1 (17 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial (30 kDa, Protein from Sus scrofa)
- Nadh:ubiquinone oxidoreductase subunit b2 (11 kDa, Protein from Sus scrofa)
- Phosphate ion (94 Da, Ligand)
- Cardiolipin (1 kDa, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 (20 kDa, Protein from Sus scrofa)
- Nadh-ubiquinone oxidoreductase chain 2 (39 kDa, Protein from Sus scrofa)
- Cytochrome b-c1 complex subunit 6, mitochondrial (10 kDa, Protein from Sus scrofa)
- Ubiquinol-cytochrome c reductase complex 7.2 kda protein (7 kDa, Protein from Sus scrofa)
- N-acetylalanine (131 Da, Ligand)
- Copper (ii) ion (63 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 (14 kDa, Protein from Sus scrofa)
- Cytochrome c oxidase subunit 7a1, mitochondrial (9 kDa, Protein from Sus scrofa)
- Nadh-ubiquinone oxidoreductase chain 1 (35 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial (21 kDa, Protein from Sus scrofa)
- Cytochrome b-c1 complex subunit 1, mitochondrial (52 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial (27 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 isoform x1 (13 kDa, Protein from Sus scrofa)
- Sodium ion (22 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial (13 kDa, Protein from Sus scrofa)
- (1s)-2-{[(2-aminoethoxy)(hydroxy)phosphoryl]oxy}-1-[(stearoyloxy)methyl]ethyl (5e,8e,11e,14e)-icosa-5,8,11,14-tetraenoate (768 Da, Ligand)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Cytochrome c oxidase subunit 5a, mitochondrial (16 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 (17 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 (11 kDa, Protein from Sus scrofa)
- Nadh:ubiquinone oxidoreductase subunit a9 (42 kDa, Protein from Sus scrofa)
- Cytochrome c oxidase subunit 5b, mitochondrial (13 kDa, Protein from Sus scrofa)
- Magnesium ion (24 Da, Ligand)
- Cytochrome b-c1 complex subunit rieske, mitochondrial (29 kDa, Protein from Sus scrofa)
- Cytochrome c oxidase subunit 7c, mitochondrial (7 kDa, Protein from Sus scrofa)
- Iron/sulfur cluster (351 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial (27 kDa, Protein from Sus scrofa)
- Cytochrome c oxidase subunit 7b (9 kDa, Protein from Sus scrofa)
- Flavin mononucleotide (456 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial (48 kDa, Protein from Sus scrofa)
- Cytochrome b-c1 complex subunit 2, mitochondrial (48 kDa, Protein from Sus scrofa)
- Cytochrome c oxidase subunit 1 (57 kDa, Protein from Sus scrofa)
- Nadh-ubiquinone oxidoreductase chain 4l (10 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 (14 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 (15 kDa, Protein from Sus scrofa)
- Cytochrome c oxidase subunit 2 (26 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial (19 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 (20 kDa, Protein from Sus scrofa)
- 1,2-distearoyl-sn-glycerophosphoethanolamine (748 Da, Ligand)
- ~{s}-[2-[3-[[(2~{r})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{s})-3-oxidanyltetradecanethioate (584 Da, Ligand)
- Dinuclear copper ion (127 Da, Ligand)
- Nadh-ubiquinone oxidoreductase chain 6 (19 kDa, Protein from Sus scrofa)
- Cytochrome c oxidase subunit ndufa4 (9 kDa, Protein from Sus scrofa)
- In-situ cryo-em structure of respiratory supercomplex by directly imaging mitochondria (Complex from Sus scrofaSus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 subunit c1, mitochondrial (8 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 (16 kDa, Protein from Sus scrofa)
- Nadh-ubiquinone oxidoreductase chain 3 (13 kDa, Protein from Sus scrofa)
- Nadh-ubiquinone oxidoreductase 75 kda subunit, mitochondrial (79 kDa, Protein from Sus scrofa)
- (1r)-2-{[{[(2s)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(palmitoyloxy)methyl]ethyl (11e)-octadec-11-enoate (749 Da, Ligand)
- Cytochrome c1 (35 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 5 (12 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 (15 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 (8 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial (50 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 (9 kDa, Protein from Sus scrofa)
- Cytochrome c oxidase subunit 4 (19 kDa, Protein from Sus scrofa)
- (7r,17e,20e)-4-hydroxy-n,n,n-trimethyl-9-oxo-7-[(palmitoyloxy)methyl]-3,5,8-trioxa-4-phosphahexacosa-17,20-dien-1-aminium 4-oxide (759 Da, Ligand)
- Potassium ion (39 Da, Ligand)
- Cytochrome b-c1 complex subunit 8 (9 kDa, Protein from Sus scrofa)
- Zinc ion (65 Da, Ligand)
- Nadh-ubiquinone oxidoreductase chain 5 (68 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial (17 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial (21 kDa, Protein from Sus scrofa)
- 1,2-diacyl-sn-glycero-3-phosphocholine (790 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial (54 kDa, Protein from Sus scrofa)
- Nadh-ubiquinone oxidoreductase chain 4 (51 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial (28 kDa, Protein from Sus scrofa)
- Cytochrome c oxidase subunit 6b1 (10 kDa, Protein from Sus scrofa)
- Nadph dihydro-nicotinamide-adenine-dinucleotide phosphate (745 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 (12 kDa, Protein from Sus scrofa)
- Heme-a (852 Da, Ligand)
- Cytochrome c oxidase subunit 6c (8 kDa, Protein from Sus scrofa)
- Cytochrome c oxidase subunit 3 (29 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial (40 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 [sus scrofa] (15 kDa, Protein from Sus scrofa)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 (11 kDa, Protein from Sus scrofa)
- Cytochrome c oxidase subunit 6a2 (10 kDa, Protein from Sus scrofa)
- Myristic acid (228 Da, Ligand)
- Cytochrome b-c1 complex subunit 7 (13 kDa, Protein from Sus scrofa)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
Cryo-EM structure of the I-II-III2-IV2 respiratory supercomplex from Tetrahymena thermophila
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 8bqs
Q-score: 0.356
Muhleip A, Flygaard RK, Baradaran R, Haapanen O, Gruhl T, Tobiasson V, Marechal A, Sharma V, Amunts A
Nature (2023) 615 pp. 934-938 [ DOI: doi:10.1038/s41586-023-05817-y Pubmed: 36949187 ]
- Surf1-like protein (35 kDa, Protein from Tetrahymena thermophila SB210)
- Nd5a (25 kDa, Protein from Tetrahymena thermophila SB210)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 (22 kDa, Protein from Tetrahymena thermophila SB210)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Ymf65 (43 kDa, Protein from Tetrahymena thermophila SB210)
- Coxtt28 (11 kDa, Protein from Tetrahymena thermophila SB210)
- Transmembrane protein, putative (15 kDa, Protein from Tetrahymena thermophila SB210)
- Ndutt11 (15 kDa, Protein from Tetrahymena thermophila SB210)
- Uqcrtt3 (5 kDa, Protein from Tetrahymena thermophila SB210)
- Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial (70 kDa, Protein from Tetrahymena thermophila SB210)
- Ndub8 (24 kDa, Protein from Tetrahymena thermophila SB210)
- Protein phosphatase 2c, putative (53 kDa, Protein from Tetrahymena thermophila SB210)
- Ndub6 (15 kDa, Protein from Tetrahymena thermophila SB210)
- Transmembrane protein, putative (11 kDa, Protein from Tetrahymena thermophila SB210)
- Chain 77 (23 kDa, Protein from Tetrahymena thermophila SB210)
- Lysozyme (12 kDa, Protein from Tetrahymena thermophila SB210)
- Potassium ion (39 Da, Ligand)
- Transmembrane protein, putative (11 kDa, Protein from Tetrahymena thermophila SB210)
- Transmembrane protein, putative (15 kDa, Protein from Tetrahymena thermophila SB210)
- Phage protein (14 kDa, Protein from Tetrahymena thermophila SB210)
- Annexin (8 kDa, Protein from Tetrahymena thermophila SB210)
- Chch domain-containing protein (13 kDa, Protein from Tetrahymena thermophila SB210)
- Ymf57 (12 kDa, Protein from Tetrahymena thermophila SB210)
- Acyl carrier protein (15 kDa, Protein from Tetrahymena thermophila SB210)
- Trab family protein (45 kDa, Protein from Tetrahymena thermophila SB210)
- Transmembrane protein, putative (26 kDa, Protein from Tetrahymena thermophila SB210)
- Nadh dehydrogenase subunit 10 (18 kDa, Protein from Tetrahymena thermophila SB210)
- Transmembrane protein, putative (8 kDa, Protein from Tetrahymena thermophila SB210)
- Nadph dihydro-nicotinamide-adenine-dinucleotide phosphate (745 Da, Ligand)
- Transmembrane protein, putative (15 kDa, Protein from Tetrahymena thermophila SB210)
- Transcription factor apfi protein, putative (39 kDa, Protein from Tetrahymena thermophila SB210)
- Cox assembly mitochondrial protein (17 kDa, Protein from Tetrahymena thermophila SB210)
- Transmembrane protein (20 kDa, Protein from Tetrahymena thermophila SB210)
- Succinate dehydrogenase (36 kDa, Protein from Tetrahymena thermophila SB210)
- Thioredoxin (19 kDa, Protein from Tetrahymena thermophila SB210)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 (15 kDa, Protein from Tetrahymena thermophila SB210)
- Iron-binding zinc finger cdgsh type protein (21 kDa, Protein from Tetrahymena thermophila SB210)
- Magnesium ion (24 Da, Ligand)
- Transmembrane protein, putative (24 kDa, Protein from Tetrahymena thermophila SB210)
- Ymf58 (13 kDa, Protein from Tetrahymena thermophila SB210)
- Transmembrane protein, putative (15 kDa, Protein from Tetrahymena thermophila SB210)
- Transmembrane protein (24 kDa, Protein from Tetrahymena thermophila SB210)
- Acyl-coa synthetase (amp-forming)/amp-acid ligase ii (36 kDa, Protein from Tetrahymena thermophila SB210)
- Zinc-finger protein (15 kDa, Protein from Tetrahymena thermophila SB210)
- Carrier protein (37 kDa, Protein from Tetrahymena thermophila SB210)
- Transmembrane protein, putative (41 kDa, Protein from Tetrahymena thermophila SB210)
- Zinc ion (65 Da, Ligand)
- Cytochrome c oxidase subunit vb protein (75 kDa, Protein from Tetrahymena thermophila SB210)
- Cytochrome c oxidase subunit 1 (80 kDa, Protein from Tetrahymena thermophila SB210)
- Coxtt12,transmembrane protein,transmembrane protein,transmembrane protein (25 kDa, Protein from Tetrahymena thermophila SB210)
- Etc complex i subunit motif protein (24 kDa, Protein from Tetrahymena thermophila SB210)
- Transmembrane protein, putative (23 kDa, Protein from Tetrahymena thermophila SB210)
- Ndua13 (21 kDa, Protein from Tetrahymena thermophila SB210)
- Ubiquinone-8 (727 Da, Ligand)
- Ndub10 (22 kDa, Protein from Tetrahymena thermophila SB210)
- Transmembrane protein, putative (11 kDa, Protein from Tetrahymena thermophila SB210)
- Transmembrane protein, putative (9 kDa, Protein from Tetrahymena thermophila SB210)
- Flavin-adenine dinucleotide (785 Da, Ligand)
- Transmembrane protein, putative (35 kDa, Protein from Tetrahymena thermophila SB210)
- Cytochrome c oxidase subunit 2 (72 kDa, Protein from Tetrahymena thermophila SB210)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial (21 kDa, Protein from Tetrahymena thermophila SB210)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 (15 kDa, Protein from Tetrahymena thermophila SB210)
- Acyl carrier protein (16 kDa, Protein from Tetrahymena thermophila SB210)
- Coxtt9 (29 kDa, Protein from Tetrahymena thermophila SB210)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial (28 kDa, Protein from Tetrahymena thermophila SB210)
- Respiratory chain supercomplex from the ciliate tetrahymena thermophila (Complex from Tetrahymena thermophila SB210)
- Transmembrane protein, putative (26 kDa, Protein from Tetrahymena thermophila SB210)
- Nadh dehydrogenase subunit 9 (23 kDa, Protein from Tetrahymena thermophila SB210)
- Gamma-carbonic anhydrase (25 kDa, Protein from Tetrahymena thermophila SB210)
- M16 family peptidase, putative (53 kDa, Protein from Tetrahymena thermophila SB210)
- Transmembrane protein, putative (7 kDa, Protein from Tetrahymena thermophila SB210)
- Cardiolipin (1 kDa, Ligand)
- Nmra domain-containing protein (36 kDa, Protein from Tetrahymena thermophila SB210)
- Transmembrane protein, putative (29 kDa, Protein from Tetrahymena thermophila SB210)
- Ribosomal protein l51/s25/ci-b8 domain protein (12 kDa, Protein from Tetrahymena thermophila SB210)
- Zf-tim10_ddp domain-containing protein (8 kDa, Protein from Tetrahymena thermophila SB210)
- Rnase iii domain-containing protein (35 kDa, Protein from Tetrahymena thermophila SB210)
- 1,2-diacyl-sn-glycero-3-phosphocholine (790 Da, Ligand)
- Transmembrane protein, putative (16 kDa, Protein from Tetrahymena thermophila SB210)
- Coxtt10 (28 kDa, Protein from Tetrahymena thermophila SB210)
- S-[2-({n-[(2r)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate (540 Da, Ligand)
- Protoporphyrin ix containing fe (616 Da, Ligand)
- Uridine-5'-diphosphate (404 Da, Ligand)
- Transmembrane protein, putative (18 kDa, Protein from Tetrahymena thermophila SB210)
- Cx9c domain-containing protein (28 kDa, Protein from Tetrahymena thermophila SB210)
- Transmembrane protein, putative (8 kDa, Protein from Tetrahymena thermophila SB210)
- Cytochrome protein c1 (37 kDa, Protein from Tetrahymena thermophila SB210)
- Transmembrane protein, putative (12 kDa, Protein from Tetrahymena thermophila SB210)
- Nadh dehydrogenase subunit 7 (51 kDa, Protein from Tetrahymena thermophila SB210)
- Ymf67 (54 kDa, Protein from Tetrahymena thermophila SB210)
- Coxtt22 (17 kDa, Protein from Tetrahymena thermophila SB210)
- Succinate dehydrogenase complex iron-sulfur subunit d (5 kDa, Protein from Tetrahymena thermophila SB210)
- Heme c (618 Da, Ligand)
- Nad-dependent epimerase/dehydratase family protein (41 kDa, Protein from Tetrahymena thermophila SB210)
- Nadh-ubiquinone oxidoreductase complex i, 21 kda subunit (17 kDa, Protein from Tetrahymena thermophila SB210)
- Surf1-like protein (47 kDa, Protein from Tetrahymena thermophila SB210)
- 37s ribosomal protein s25, mitochondrial (33 kDa, Protein from Tetrahymena thermophila SB210)
- Ctf/nf-i domain-containing protein (116 kDa, Protein from Tetrahymena thermophila SB210)
- Complex iii subunit vii (19 kDa, Protein from Tetrahymena thermophila SB210)
- Rieske iron-sulfur protein, ubiquinol-cytochrome c reductase iron-sulfur subunit (30 kDa, Protein from Tetrahymena thermophila SB210)
- Calcium ion (40 Da, Ligand)
- Copper (ii) ion (63 Da, Ligand)
- Lipid-a-disaccharide synthase (59 kDa, Protein from Tetrahymena thermophila SB210)
- Sdhtt11 (5 kDa, Protein from Tetrahymena thermophila SB210)
- Transposase (11 kDa, Protein from Tetrahymena thermophila SB210)
- Transmembrane protein (7 kDa, Protein from Tetrahymena thermophila SB210)
- 2-oxoglutarate/malate carrier protein (36 kDa, Protein from Tetrahymena thermophila SB210)
- Transmembrane protein, putative (24 kDa, Protein from Tetrahymena thermophila SB210)
- Duf4885 domain-containing protein (12 kDa, Protein from Tetrahymena thermophila SB210)
- Ymf68 (72 kDa, Protein from Tetrahymena thermophila SB210)
- Ndub4 (14 kDa, Protein from Tetrahymena thermophila SB210)
- Abc transporter (10 kDa, Protein from Tetrahymena thermophila SB210)
- 39s ribosomal protein l9, mitochondrial (27 kDa, Protein from Tetrahymena thermophila SB210)
- Nadh dehydrogenase, putative (20 kDa, Protein from Tetrahymena thermophila SB210)
- Transmembrane protein, putative (14 kDa, Protein from Tetrahymena thermophila SB210)
- Ph domain-containing protein (15 kDa, Protein from Tetrahymena thermophila SB210)
- Gamma-carbonic anhydrase (28 kDa, Protein from Tetrahymena thermophila SB210)
- Transmembrane protein, putative (9 kDa, Protein from Tetrahymena thermophila SB210)
- Fe3-s4 cluster (295 Da, Ligand)
- Heme-a (852 Da, Ligand)
- 2 iron, 2 sulfur cluster-binding protein (19 kDa, Protein from Tetrahymena thermophila SB210)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial (22 kDa, Protein from Tetrahymena thermophila SB210)
- 2-(hexadecanoyloxy)-1-[(phosphonooxy)methyl]ethyl hexadecanoate (648 Da, Ligand)
- Nadh-ubiquinone oxidoreductase 24 kda subunit (31 kDa, Protein from Tetrahymena thermophila SB210)
- Nadh dehydrogenase subunit 1 (7 kDa, Protein from Tetrahymena thermophila SB210)
- Peptidase m16 inactive domain protein (58 kDa, Protein from Tetrahymena thermophila SB210)
- Zf-tim10_ddp domain-containing protein (10 kDa, Protein from Tetrahymena thermophila SB210)
- Nadh dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial (52 kDa, Protein from Tetrahymena thermophila SB210)
- Cullin domain-containing protein (8 kDa, Protein from Tetrahymena thermophila SB210)
- Ymf62 (30 kDa, Protein from Tetrahymena thermophila SB210)
- Nduph2 (22 kDa, Protein from Tetrahymena thermophila SB210)
- Nadh-ubiquinone oxidoreductase 1, chain, putative (28 kDa, Protein from Tetrahymena thermophila SB210)
- Ubiquinol-cytochrome c reductase hinge protein (9 kDa, Protein from Tetrahymena thermophila SB210)
- Decapping nuclease (10 kDa, Protein from Tetrahymena thermophila SB210)
- Nadh-ubiquinone oxidoreductase chain 4 (58 kDa, Protein from Tetrahymena thermophila SB210)
- Oxoglutarate/malate translocator protein, putative (40 kDa, Protein from Tetrahymena thermophila SB210)
- Transmembrane protein, putative (16 kDa, Protein from Tetrahymena thermophila SB210)
- Diphthamide synthesis protein (22 kDa, Protein from Tetrahymena thermophila SB210)
- Tim10/ddp family zinc finger protein (8 kDa, Protein from Tetrahymena thermophila SB210)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8, mitochondrial (37 kDa, Protein from Tetrahymena thermophila SB210)
- Nadh dehydrogenase subunit 2 (20 kDa, Protein from Tetrahymena thermophila SB210)
- Apocytochrome b (50 kDa, Protein from Tetrahymena thermophila SB210)
- Ymf70 (10 kDa, Protein from Tetrahymena thermophila SB210)
- Transposase (8 kDa, Protein from Tetrahymena thermophila SB210)
- Chromosome condensation regulator rcc1 repeat protein,chromosome condensation regulator rcc1 repeat protein,chromosome condensation regulator rcc1 repeat protein,chromosome condensation regulator rcc1 repeat protein,chain 150 (50 kDa, Protein from Tetrahymena thermophila SB210)
- Nadh-ubiquinone oxidoreductase chain 1 (32 kDa, Protein from Tetrahymena thermophila SB210)
- Dinuclear copper ion (127 Da, Ligand)
- Dnaj domain protein (36 kDa, Protein from Tetrahymena thermophila SB210)
- Nadh dehydrogenase subunit 5 (88 kDa, Protein from Tetrahymena thermophila SB210)
- Structural protein (27 kDa, Protein from Tetrahymena thermophila SB210)
- Uqcrtt2 (4 kDa, Protein from Tetrahymena thermophila SB210)
- Nadh-ubiquinone oxidoreductase 75 kda subunit (80 kDa, Protein from Tetrahymena thermophila SB210)
- Mobilization protein (23 kDa, Protein from Tetrahymena thermophila SB210)
- Transmembrane protein, putative (20 kDa, Protein from Tetrahymena thermophila SB210)
- Transmembrane protein, putative (18 kDa, Protein from Tetrahymena thermophila SB210)
- Ndutt10 (14 kDa, Protein from Tetrahymena thermophila SB210)
- Nadh-ubiquinone oxidoreductase chain 3 (14 kDa, Protein from Tetrahymena thermophila SB210)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Transmembrane protein, putative (14 kDa, Protein from Tetrahymena thermophila SB210)
- Iron/sulfur cluster (351 Da, Ligand)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Flavin mononucleotide (456 Da, Ligand)
- Uqcrtt1 (39 kDa, Protein from Tetrahymena thermophila SB210)
- Ndutt12 (13 kDa, Protein from Tetrahymena thermophila SB210)
- Transmembrane protein, putative (7 kDa, Protein from Tetrahymena thermophila SB210)
- Yflt domain-containing protein (19 kDa, Protein from Tetrahymena thermophila SB210)
- 1,2-distearoyl-sn-glycerophosphoethanolamine (748 Da, Ligand)
- Transmembrane protein, putative (13 kDa, Protein from Tetrahymena thermophila SB210)
- Coxtt27 (23 kDa, Protein from Tetrahymena thermophila SB210)
- Atp synthase subunit e, mitochondrial (11 kDa, Protein from Tetrahymena thermophila SB210)
- Complex i-mnll (12 kDa, Protein from Tetrahymena thermophila SB210)
- Gram domain protein (10 kDa, Protein from Tetrahymena thermophila SB210)
- Transmembrane protein, putative (57 kDa, Protein from Tetrahymena thermophila SB210)
- Ymf75 (23 kDa, Protein from Tetrahymena thermophila SB210)
Resolution: 2.6 Å
EM Method: Single-particle
Fitted PDBs: 8b6h
Q-score: 0.531
Muhleip A, Flygaard RK, Baradaran R, Haapanen O, Gruhl T, Tobiasson V, Marechal A, Sharma V, Amunts A
Nature (2023) 615 pp. 934-938 [ DOI: doi:10.1038/s41586-023-05817-y Pubmed: 36949187 ]
- 1,2-diacyl-sn-glycero-3-phosphocholine (790 Da, Ligand)
- Cytochrome c oxidase dimer (complex-iv) from respiratory supercomplex of tetrahymena thermophila (3 MDa, Complex from Tetrahymena thermophila)
- Cytochrome c oxidase subunit 2 (72 kDa, Protein from Tetrahymena thermophila SB210)
- Zf-tim10_ddp domain-containing protein (8 kDa, Protein from Tetrahymena thermophila SB210)
- Mobilization protein (23 kDa, Protein from Tetrahymena thermophila SB210)
- Transmembrane protein, putative (18 kDa, Protein from Tetrahymena thermophila SB210)
- Dinuclear copper ion (127 Da, Ligand)
- Protoporphyrin ix containing fe (616 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial (28 kDa, Protein from Tetrahymena thermophila SB210)
- Transmembrane protein, putative (12 kDa, Protein from Tetrahymena thermophila SB210)
- Iron-binding zinc finger cdgsh type protein (21 kDa, Protein from Tetrahymena thermophila SB210)
- Yflt domain-containing protein (19 kDa, Protein from Tetrahymena thermophila SB210)
- Abc transporter (10 kDa, Protein from Tetrahymena thermophila SB210)
- Copper (ii) ion (63 Da, Ligand)
- Coxtt10 (28 kDa, Protein from Tetrahymena thermophila SB210)
- Surf1-like protein (35 kDa, Protein from Tetrahymena thermophila SB210)
- Structural protein (27 kDa, Protein from Tetrahymena thermophila SB210)
- Carrier protein (37 kDa, Protein from Tetrahymena thermophila SB210)
- Coxtt27 (23 kDa, Protein from Tetrahymena thermophila SB210)
- Cytochrome c oxidase subunit vb protein (75 kDa, Protein from Tetrahymena thermophila SB210)
- Transmembrane protein, putative (15 kDa, Protein from Tetrahymena thermophila SB210)
- Coxtt12,transmembrane protein,transmembrane protein (25 kDa, Protein from Tetrahymena thermophila SB210)
- Annexin (8 kDa, Protein from Tetrahymena thermophila SB210)
- Cardiolipin (1 kDa, Ligand)
- Zinc ion (65 Da, Ligand)
- Phage protein (14 kDa, Protein from Tetrahymena thermophila SB210)
- Surf1-like protein (47 kDa, Protein from Tetrahymena thermophila SB210)
- Zf-tim10_ddp domain-containing protein (10 kDa, Protein from Tetrahymena thermophila SB210)
- Heme-a (852 Da, Ligand)
- Protein phosphatase 2c, putative (53 kDa, Protein from Tetrahymena thermophila SB210)
- Complex iii subunit vii (19 kDa, Protein from Tetrahymena thermophila SB210)
- Transmembrane protein, putative (57 kDa, Protein from Tetrahymena thermophila SB210)
- Transmembrane protein (20 kDa, Protein from Tetrahymena thermophila SB210)
- Decapping nuclease (10 kDa, Protein from Tetrahymena thermophila SB210)
- Lysozyme (12 kDa, Protein from Tetrahymena thermophila SB210)
- Calcium ion (40 Da, Ligand)
- Transmembrane protein, putative (14 kDa, Protein from Tetrahymena thermophila SB210)
- Coxtt9 (29 kDa, Protein from Tetrahymena thermophila SB210)
- Potassium ion (39 Da, Ligand)
- Coxtt28 (11 kDa, Protein from Tetrahymena thermophila SB210)
- Magnesium ion (24 Da, Ligand)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Ymf75 (23 kDa, Protein from Tetrahymena thermophila SB210)
- 39s ribosomal protein l9, mitochondrial (27 kDa, Protein from Tetrahymena thermophila SB210)
- Trab family protein (45 kDa, Protein from Tetrahymena thermophila SB210)
- Transmembrane protein, putative (41 kDa, Protein from Tetrahymena thermophila SB210)
- Cullin domain-containing protein (8 kDa, Protein from Tetrahymena thermophila SB210)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Cytochrome c oxidase subunit 1 (80 kDa, Protein from Tetrahymena thermophila SB210)
- Ctf/nf-i domain-containing protein (116 kDa, Protein from Tetrahymena thermophila SB210)
- Transmembrane protein, putative (24 kDa, Protein from Tetrahymena thermophila SB210)
- Coxbp,chromosome condensation regulator rcc1 repeat protein,chromosome condensation regulator rcc1 repeat protein (58 kDa, Protein from Tetrahymena thermophila SB210)
- Transmembrane protein, putative (16 kDa, Protein from Tetrahymena thermophila SB210)
- Ymf67 (54 kDa, Protein from Tetrahymena thermophila SB210)
- 2-oxoglutarate/malate carrier protein (36 kDa, Protein from Tetrahymena thermophila SB210)
- Tim10/ddp family zinc finger protein (8 kDa, Protein from Tetrahymena thermophila SB210)
- Transmembrane protein, putative (20 kDa, Protein from Tetrahymena thermophila SB210)
- 1,2-distearoyl-sn-glycerophosphoethanolamine (748 Da, Ligand)
- Ubiquinone-8 (727 Da, Ligand)
- Transposase (8 kDa, Protein from Tetrahymena thermophila SB210)
- Transmembrane protein, putative (15 kDa, Protein from Tetrahymena thermophila SB210)
- Ymf68 (72 kDa, Protein from Tetrahymena thermophila SB210)
- Transmembrane protein, putative (26 kDa, Protein from Tetrahymena thermophila SB210)
- Coxtt22 (17 kDa, Protein from Tetrahymena thermophila SB210)
- Transmembrane protein, putative (18 kDa, Protein from Tetrahymena thermophila SB210)
- Ymf70 (10 kDa, Protein from Tetrahymena thermophila SB210)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8, mitochondrial (37 kDa, Protein from Tetrahymena thermophila SB210)
- 2-(hexadecanoyloxy)-1-[(phosphonooxy)methyl]ethyl hexadecanoate (648 Da, Ligand)
- Oxoglutarate/malate translocator protein, putative (40 kDa, Protein from Tetrahymena thermophila SB210)
Murine supercomplex CIII2CIV in the assembled locked conformation
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7o37
Q-score: 0.515
Nature (2021) 598 pp. 364-367 [ Pubmed: 34616041 DOI: doi:10.1038/s41586-021-03927-z ]
- Cytochrome b-c1 complex subunit 6, mitochondrial (9 kDa, Protein from Mus musculus)
- Cytochrome c oxidase subunit 7b, mitochondrial (6 kDa, Protein from Mus musculus)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Cytochrome b-c1 complex subunit 7 (13 kDa, Protein from Mus musculus)
- Cytochrome c oxidase subunit 6a2, mitochondrial (9 kDa, Protein from Mus musculus)
- Protoporphyrin ix containing fe (616 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Cytochrome c oxidase subunit 5b, mitochondrial (10 kDa, Protein from Mus musculus)
- Sodium ion (22 Da, Ligand)
- Heme c (618 Da, Ligand)
- 1,2-distearoyl-sn-glycerophosphoethanolamine (748 Da, Ligand)
- Dinuclear copper ion (127 Da, Ligand)
- Cytochrome b-c1 complex subunit 1, mitochondrial (49 kDa, Protein from Mus musculus)
- Cytochrome b-c1 complex subunit 2, mitochondrial (46 kDa, Protein from Mus musculus)
- Murine full supercomplex ciii2civ in the locked conformation (700 kDa, Complex from Mus musculus)
- Cytochrome b-c1 complex subunit 8 (9 kDa, Protein from Mus musculus)
- Cytochrome b-c1 complex subunit 10 (6 kDa, Protein from Mus musculus)
- Tristearoylglycerol (891 Da, Ligand)
- 1,2-diacyl-sn-glycero-3-phosphocholine (790 Da, Ligand)
- Cytochrome c oxidase subunit 3 (29 kDa, Protein from Mus musculus)
- Cardiolipin (1 kDa, Ligand)
- Cytochrome c oxidase subunit 2 (25 kDa, Protein from Mus musculus)
- Cytochrome c oxidase subunit 5a, mitochondrial (12 kDa, Protein from Mus musculus)
- Cytochrome c1, heme protein, mitochondrial (27 kDa, Protein from Mus musculus)
- Cytochrome c oxidase subunit 6c (8 kDa, Protein from Mus musculus)
- Cytochrome b-c1 complex subunit rieske, mitochondrial (21 kDa, Protein from Mus musculus)
- Cytochrome b-c1 complex subunit 9 (7 kDa, Protein from Mus musculus)
- Copper (ii) ion (63 Da, Ligand)
- Cytochrome b-c1 complex subunit 9 (7 kDa, Protein from Mus musculus)
- Cytochrome c oxidase subunit 4 isoform 1, mitochondrial (17 kDa, Protein from Mus musculus)
- Heme-a (852 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Cytochrome c oxidase subunit 8b, mitochondrial (4 kDa, Protein from Mus musculus)
- Cox7a2l protein (12 kDa, Protein from Mus musculus)
- Cytochrome b (43 kDa, Protein from Mus musculus)
- Cytochrome c oxidase subunit 6b1 (9 kDa, Protein from Mus musculus)
- Cytochrome c oxidase subunit 1 (56 kDa, Protein from Mus musculus)
- Cytochrome c oxidase subunit 7c, mitochondrial (5 kDa, Protein from Mus musculus)
Murine supercomplex CIII2CIV in the mature unlocked conformation
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7o3c
Q-score: 0.493
Nature (2021) 598 pp. 364-367 [ Pubmed: 34616041 DOI: doi:10.1038/s41586-021-03927-z ]
- Cytochrome b-c1 complex subunit 6, mitochondrial (9 kDa, Protein from Mus musculus)
- Cytochrome c oxidase subunit 7b, mitochondrial (6 kDa, Protein from Mus musculus)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Cytochrome b-c1 complex subunit 7 (13 kDa, Protein from Mus musculus)
- Cytochrome c oxidase subunit 6a2, mitochondrial (9 kDa, Protein from Mus musculus)
- Protoporphyrin ix containing fe (616 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Cytochrome c oxidase subunit 5b, mitochondrial (10 kDa, Protein from Mus musculus)
- Sodium ion (22 Da, Ligand)
- Heme c (618 Da, Ligand)
- 1,2-distearoyl-sn-glycerophosphoethanolamine (748 Da, Ligand)
- Murine full supercomplex ciii2civ in the unlocked conformation (700 kDa, Complex from Mus musculus)
- Dinuclear copper ion (127 Da, Ligand)
- Cytochrome b-c1 complex subunit 1, mitochondrial (49 kDa, Protein from Mus musculus)
- Cytochrome b-c1 complex subunit 2, mitochondrial (46 kDa, Protein from Mus musculus)
- Cytochrome b-c1 complex subunit 8 (9 kDa, Protein from Mus musculus)
- Cytochrome b-c1 complex subunit 10 (6 kDa, Protein from Mus musculus)
- Tristearoylglycerol (891 Da, Ligand)
- 1,2-diacyl-sn-glycero-3-phosphocholine (790 Da, Ligand)
- Cytochrome c oxidase subunit 3 (29 kDa, Protein from Mus musculus)
- Cardiolipin (1 kDa, Ligand)
- Cytochrome c oxidase subunit 2 (25 kDa, Protein from Mus musculus)
- Cytochrome c oxidase subunit 5a, mitochondrial (12 kDa, Protein from Mus musculus)
- Cytochrome c1, heme protein, mitochondrial (27 kDa, Protein from Mus musculus)
- Cytochrome c oxidase subunit 6c (8 kDa, Protein from Mus musculus)
- Cytochrome b-c1 complex subunit rieske, mitochondrial (21 kDa, Protein from Mus musculus)
- Cytochrome b-c1 complex subunit 9 (7 kDa, Protein from Mus musculus)
- Copper (ii) ion (63 Da, Ligand)
- Cytochrome b-c1 complex subunit 9 (7 kDa, Protein from Mus musculus)
- Cytochrome c oxidase subunit 4 isoform 1, mitochondrial (17 kDa, Protein from Mus musculus)
- Heme-a (852 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Cytochrome c oxidase subunit 8b, mitochondrial (4 kDa, Protein from Mus musculus)
- Cox7a2l protein (12 kDa, Protein from Mus musculus)
- Cytochrome b (43 kDa, Protein from Mus musculus)
- Cytochrome c oxidase subunit 6b1 (9 kDa, Protein from Mus musculus)
- Cytochrome c oxidase subunit 1 (56 kDa, Protein from Mus musculus)
- Cytochrome c oxidase subunit 7c, mitochondrial (5 kDa, Protein from Mus musculus)