particulate methane monooxygenase crosslinked with 2,2,2-trifluoroethanol bound
Resolution: 2.16 Å
EM Method: Single-particle
Fitted PDBs: 8sqw
Q-score: 0.609
Tucci FJ, Jodts RJ, Hoffman BM, Rosenzweig AC
Nat Catal (2023) 6 pp. 1194-1204 [ DOI: doi:10.1038/s41929-023-01051-x Pubmed: 38187819 ]
- Particulate methane monooxygenase (337 kDa, Complex from Methylococcus capsulatus str. Bath)
- Diundecyl phosphatidyl choline (622 Da, Ligand)
- Trifluoroethanol (100 Da, Ligand)
- Ammonia monooxygenase/methane monooxygenase, subunit c family protein (27 kDa, Protein from Methylococcus capsulatus)
- Decane (142 Da, Ligand)
- Particulate methane monooxygenase beta subunit (27 kDa, Protein from Methylococcus capsulatus)
- 1,2-dihexanoyl-sn-glycero-3-phosphocholine (454 Da, Ligand)
- Copper (ii) ion (63 Da, Ligand)
- Particulate methane monooxygenase alpha subunit (42 kDa, Protein from Methylococcus capsulatus)
- 1,2-didecanoyl-sn-glycero-3-phosphocholine (566 Da, Ligand)
- Water (18 Da, Ligand)
Resolution: 2.14 Å
EM Method: Single-particle
Fitted PDBs: 7s4h
Q-score: 0.684
Koo CW, Tucci FJ, He Y, Rosenzweig AC
Science (2022) 375 pp. 1287-1291 [ DOI: doi:10.1126/science.abm3282 Pubmed: 35298269 ]
- Particulate methane monooxygenase alpha subunit (46 kDa, Protein from Methylococcus capsulatus str. Bath)
- Pmmo complex in a native lipid nanodisc (Complex from Methylococcus capsulatus (strain ATCC 33009 / NCIMB 11132 / Bath))
- Diundecyl phosphatidyl choline (622 Da, Ligand)
- Ammonia monooxygenase/methane monooxygenase, subunit c family protein (29 kDa, Protein from Methylococcus capsulatus str. Bath)
- Particulate methane monooxygenase beta subunit (28 kDa, Protein from Methylococcus capsulatus str. Bath)
- Decane (142 Da, Ligand)
- 1,2-dihexanoyl-sn-glycero-3-phosphocholine (454 Da, Ligand)
- Copper (ii) ion (63 Da, Ligand)
- 1,2-didecanoyl-sn-glycero-3-phosphocholine (566 Da, Ligand)
- Water (18 Da, Ligand)
Resolution: 2.26 Å
EM Method: Single-particle
Fitted PDBs: 7s4i
Q-score: 0.679
Koo CW, Tucci FJ, He Y, Rosenzweig AC
Science (2022) 375 pp. 1287-1291 [ DOI: doi:10.1126/science.abm3282 Pubmed: 35298269 ]
- Particulate methane monooxygenase alpha subunit (46 kDa, Protein from Methylococcus capsulatus str. Bath)
- Pmmo complex in a native lipid nanodisc (Complex from Methylococcus capsulatus (strain ATCC 33009 / NCIMB 11132 / Bath))
- Diundecyl phosphatidyl choline (622 Da, Ligand)
- Ammonia monooxygenase/methane monooxygenase, subunit c family protein (29 kDa, Protein from Methylococcus capsulatus str. Bath)
- Particulate methane monooxygenase beta subunit (28 kDa, Protein from Methylococcus capsulatus str. Bath)
- Decane (142 Da, Ligand)
- 1,2-dihexanoyl-sn-glycero-3-phosphocholine (454 Da, Ligand)
- Copper (ii) ion (63 Da, Ligand)
- 1,2-didecanoyl-sn-glycero-3-phosphocholine (566 Da, Ligand)
- Water (18 Da, Ligand)
Resolution: 2.16 Å
EM Method: Single-particle
Fitted PDBs: 7s4j
Q-score: 0.66
Koo CW, Tucci FJ, He Y, Rosenzweig AC
Science (2022) 375 pp. 1287-1291 [ DOI: doi:10.1126/science.abm3282 Pubmed: 35298269 ]
- Particulate methane monooxygenase alpha subunit (46 kDa, Protein from Methylococcus capsulatus str. Bath)
- Pmmo complex in a native lipid nanodisc (Complex from Methylococcus capsulatus (strain ATCC 33009 / NCIMB 11132 / Bath))
- Diundecyl phosphatidyl choline (622 Da, Ligand)
- Ammonia monooxygenase/methane monooxygenase, subunit c family protein (29 kDa, Protein from Methylococcus capsulatus str. Bath)
- Particulate methane monooxygenase beta subunit (28 kDa, Protein from Methylococcus capsulatus str. Bath)
- Decane (142 Da, Ligand)
- 1,2-dihexanoyl-sn-glycero-3-phosphocholine (454 Da, Ligand)
- Copper (ii) ion (63 Da, Ligand)
- 1,2-didecanoyl-sn-glycero-3-phosphocholine (566 Da, Ligand)
- Water (18 Da, Ligand)
Resolution: 2.36 Å
EM Method: Single-particle
Fitted PDBs: 7s4k
Q-score: 0.636
Koo CW, Tucci FJ, He Y, Rosenzweig AC
Science (2022) 375 pp. 1287-1291 [ DOI: doi:10.1126/science.abm3282 Pubmed: 35298269 ]
- Particulate methane monooxygenase alpha subunit (46 kDa, Protein from Methylococcus capsulatus str. Bath)
- Pmmo complex in a native lipid nanodisc (Complex from Methylococcus capsulatus (strain ATCC 33009 / NCIMB 11132 / Bath))
- Diundecyl phosphatidyl choline (622 Da, Ligand)
- Ammonia monooxygenase/methane monooxygenase, subunit c family protein (29 kDa, Protein from Methylococcus capsulatus str. Bath)
- Particulate methane monooxygenase beta subunit (28 kDa, Protein from Methylococcus capsulatus str. Bath)
- Decane (142 Da, Ligand)
- 1,2-dihexanoyl-sn-glycero-3-phosphocholine (454 Da, Ligand)
- Copper (ii) ion (63 Da, Ligand)
- 1,2-didecanoyl-sn-glycero-3-phosphocholine (566 Da, Ligand)
- Water (18 Da, Ligand)
Resolution: 2.46 Å
EM Method: Single-particle
Fitted PDBs: 7s4l
Q-score: 0.564
Koo CW, Tucci FJ, He Y, Rosenzweig AC
Science (2022) 375 pp. 1287-1291 [ DOI: doi:10.1126/science.abm3282 Pubmed: 35298269 ]
- (s)-2,3-bis(hexanoyloxy)propyl(2-(trimethylammonio)ethyl)phosphate (454 Da, Ligand)
- Particulate methane monooxygenase, a subunit (28 kDa, Protein from Methylomicrobium alcaliphilum (strain DSM 19304 / NCIMB 14124 / VKM B-2133 / 20Z))
- Particulate methane monooxygenase, b subunit (45 kDa, Protein from Methylomicrobium alcaliphilum (strain DSM 19304 / NCIMB 14124 / VKM B-2133 / 20Z))
- Pmmo complex in a popc nanodisc (Complex from Methylotuvimicrobium alcaliphilum 20Z)
- Decane (142 Da, Ligand)
- 1,2-dihexanoyl-sn-glycero-3-phosphocholine (454 Da, Ligand)
- Copper (ii) ion (63 Da, Ligand)
- Water (18 Da, Ligand)
- Particulate methane monooxygenase, c subunit (28 kDa, Protein from Methylomicrobium alcaliphilum (strain DSM 19304 / NCIMB 14124 / VKM B-2133 / 20Z))
Resolution: 2.42 Å
EM Method: Single-particle
Fitted PDBs: 7s4m
Q-score: 0.461
Koo CW, Tucci FJ, He Y, Rosenzweig AC
Science (2022) 375 pp. 1287-1291 [ DOI: doi:10.1126/science.abm3282 Pubmed: 35298269 ]
- Pmmo complex in a native lipid nanodisc (Complex from Methylocystis sp. ATCC 49242)
- Ammonia monooxygenase/methane monooxygenase, subunit c family protein (27 kDa, Protein from Methylocystis sp. ATCC 49242)
- Decane (142 Da, Ligand)
- Particulate methane monooxygenase alpha subunit (42 kDa, Protein from Methylocystis sp. ATCC 49242)
- Particulate methane monooxygenase beta subunit (27 kDa, Protein from Methylocystis sp. ATCC 49242)
- Copper (ii) ion (63 Da, Ligand)
- 1,2-dihexanoyl-sn-glycero-3-phosphocholine (454 Da, Ligand)
- Unidentified helix (1 kDa, Protein from Methylocystis sp. ATCC 49242)
- Water (18 Da, Ligand)
- Dodecane (170 Da, Ligand)
Resolution: 3.65 Å
EM Method: Single-particle
Fitted PDBs: 7t4o
Q-score: 0.428
Koo CW, Tucci FJ, He Y, Rosenzweig AC
Science (2022) 375 pp. 1287-1291 [ DOI: doi:10.1126/science.abm3282 Pubmed: 35298269 ]
- Particulate methane monooxygenase alpha subunit (46 kDa, Protein from Methylococcus capsulatus str. Bath)
- Pmmo complex in a native lipid nanodisc (Complex from Methylococcus capsulatus str. Bath)
- Diundecyl phosphatidyl choline (622 Da, Ligand)
- Ammonia monooxygenase/methane monooxygenase, subunit c family protein (29 kDa, Protein from Methylococcus capsulatus str. Bath)
- Particulate methane monooxygenase beta subunit (28 kDa, Protein from Methylococcus capsulatus str. Bath)
- Decane (142 Da, Ligand)
- Copper (ii) ion (63 Da, Ligand)
- 1,2-didecanoyl-sn-glycero-3-phosphocholine (566 Da, Ligand)
Resolution: 3.62 Å
EM Method: Single-particle
Fitted PDBs: 7t4p
Q-score: 0.509
Koo CW, Tucci FJ, He Y, Rosenzweig AC
Science (2022) 375 pp. 1287-1291 [ DOI: doi:10.1126/science.abm3282 Pubmed: 35298269 ]
- Particulate methane monooxygenase alpha subunit (46 kDa, Protein from Methylococcus capsulatus str. Bath)
- Pmmo complex in a native lipid nanodisc (Complex from Methylococcus capsulatus str. Bath)
- Diundecyl phosphatidyl choline (622 Da, Ligand)
- Ammonia monooxygenase/methane monooxygenase, subunit c family protein (29 kDa, Protein from Methylococcus capsulatus str. Bath)
- Particulate methane monooxygenase beta subunit (28 kDa, Protein from Methylococcus capsulatus str. Bath)
- Decane (142 Da, Ligand)
- 1,2-dihexanoyl-sn-glycero-3-phosphocholine (454 Da, Ligand)
- Copper (ii) ion (63 Da, Ligand)
- 1,2-didecanoyl-sn-glycero-3-phosphocholine (566 Da, Ligand)