Structure of GABAAR determined by cryoEM at 100 keV
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 8pvb
Q-score: 0.486
McMullan G, Naydenova K, Mihaylov D, Yamashita K, Peet MJ, Wilson H, Dickerson JL, Chen S, Cannone G, Lee Y, Hutchings KA, Gittins O, Sobhy MA, Wells T, El-Gomati MM, Dalby J, Meffert M, Schulze-Briese C, Henderson R, Russo CJ
PNAS (2023) 120 pp. e2312905120-e2312905120 [ Pubmed: 38011573 DOI: doi:10.1073/pnas.2312905120 ]
- Human gaba(a)r-beta3 homopentamer in complex with megabody-25 in lipid nanodisc (Complex from Homo sapiens)
- Histamine (111 Da, Ligand)
- Hexadecane (226 Da, Ligand)
- Decane (142 Da, Ligand)
- Gamma-aminobutyric acid receptor subunit beta-3 (45 kDa, Protein from Homo sapiens)
- Megabody mb25 (56 kDa, Protein from Lama glama)
- Etomidate (244 Da, Ligand)
- Chloride ion (35 Da, Ligand)
Resolution: 2.5 Å
EM Method: Single-particle
Fitted PDBs: 7qn5
Q-score: 0.58
Sente A, Desai R, Naydenova K, Malinauskas T, Jounaidi Y, Miehling J, Zhou X, Masiulis S, Hardwick SW, Chirgadze DY, Miller KW, Aricescu AR
Nature (2022) 604 pp. 190-194 [ Pubmed: 35355020 DOI: doi:10.1038/s41586-022-04517-3 ]
- 1,2-dipalmitoyl-sn-glycero-3-phosphate (647 Da, Ligand)
- Gamma-aminobutyric acid receptor subunit beta-3 (54 kDa, Protein from Homo sapiens)
- Nanobody nb25 (13 kDa, Protein from Lama glama)
- Human full-length extrasynaptic alpha4beta3delta gaba(a)r in complex with nanobody nb25 (Complex from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Gamma-aminobutyric acid receptor subunit alpha-4 (61 kDa, Protein from Homo sapiens)
- 4-(2-hydroxyethyl)-1-piperazine ethanesulfonic acid (238 Da, Ligand)
- Decane (142 Da, Ligand)
- N-octane (114 Da, Ligand)
- Gamma-aminobutyric acid receptor subunit delta (52 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Chloride ion (35 Da, Ligand)
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7qnc
Q-score: 0.523
Sente A, Desai R, Naydenova K, Malinauskas T, Jounaidi Y, Miehling J, Zhou X, Masiulis S, Hardwick SW, Chirgadze DY, Miller KW, Aricescu AR
Nature (2022) 604 pp. 190-194 [ Pubmed: 35355020 DOI: doi:10.1038/s41586-022-04517-3 ]
- Gamma-aminobutyric acid receptor subunit beta-3 (54 kDa, Protein from Homo sapiens)
- Nanobody nb25 (13 kDa, Protein from Lama glama)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Human full-length extrasynaptic alpha4beta3delta gaba(a)r in complex with thip (gaboxadol), histamine and nanobody nb25 (Complex from Homo sapiens)
- Gamma-aminobutyric acid receptor subunit alpha-4 (61 kDa, Protein from Homo sapiens)
- Histamine (111 Da, Ligand)
- Decane (142 Da, Ligand)
- Gamma-aminobutyric acid receptor subunit delta (52 kDa, Protein from Homo sapiens)
- 4,5,6,7-tetrahydro-[1,2]oxazolo[5,4-c]pyridin-3-one (140 Da, Ligand)
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 7qne
Q-score: 0.606
Sente A, Desai R, Naydenova K, Malinauskas T, Jounaidi Y, Miehling J, Zhou X, Masiulis S, Hardwick SW, Chirgadze DY, Miller KW, Aricescu AR
Nature (2022) 604 pp. 190-194 [ Pubmed: 35355020 DOI: doi:10.1038/s41586-022-04517-3 ]
- Gamma-aminobutyric acid receptor subunit beta-3 (54 kDa, Protein from Homo sapiens)
- Megabody mb38 (13 kDa, Protein from Lama glama)
- [(2r)-2-octanoyloxy-3-[oxidanyl-[(1r,2r,3s,4r,5r,6s)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate (746 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Ethyl 8-[(azanylidene-$l^{4}-azanylidene)amino]-5-methyl-6-oxidanylidene-4~{h}-imidazo[1,5-a][1,4]benzodiazepine-3-carboxylate (326 Da, Ligand)
- Gaba(a) receptor subunit gamma-2 (56 kDa, Protein from Homo sapiens)
- Decane (142 Da, Ligand)
- N-octane (114 Da, Ligand)
- Gaba(a) receptor subunit alpha-1 (51 kDa, Protein from Homo sapiens)
- Human full-length synaptic alpha1beta3gamma2 gaba(a)r in complex with ro15-4513 and megabody mb38 (Complex from Homo sapiens)
- Chloride ion (35 Da, Ligand)
Resolution: 2.79 Å
EM Method: Single-particle
Fitted PDBs: 7pbz
Q-score: 0.548
Kasaragod VB, Mortensen M, Hardwick SW, Wahid AA, Dorovykh V, Chirgadze DY, Smart TG, Miller PS
Nature (2022) 602 pp. 529-533 [ DOI: doi:10.1038/s41586-022-04402-z Pubmed: 35140402 ]
- Gamma-aminobutyric acid receptor subunit alpha-1 (47 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Hexane (86 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Gamma-aminobutyric acid receptor subunit alpha-1 (41 kDa, Protein from Homo sapiens)
- Gaba-a receptor - megabody 25 complex (220 kDa, Complex)
- Undecane (156 Da, Ligand)
- Megabody 25 (Complex from Lama glama)
- Gamma-amino-butanoic acid (103 Da, Ligand)
- Histamine (111 Da, Ligand)
- Decane (142 Da, Ligand)
- N-octane (114 Da, Ligand)
- Gamma-aminobutyric acid receptor subunit beta-3 (51 kDa, Protein from Homo sapiens)
- Gaba-a receptor (Complex from Homo sapiens)
- Megabody 25 (56 kDa, Protein from Lama glama)
Resolution: 1.7 Å
EM Method: Single-particle
Fitted PDBs: 7a5v
Q-score: 0.75
Nakane T, Kotecha A, Sente A, McMullan G, Masiulis S, Brown PMGE, Grigoras IT, Malinauskaite L, Malinauskas T, Miehling J, Uchanski T, Yu L, Karia D, Pechnikova EV, de Jong E, Keizer J, Bischoff M, McCormack J, Tiemeijer P, Hardwick SW, Chirgadze DY, Murshudov G, Aricescu AR, Scheres SHW
Nature (2020) 587 pp. 152-156 [ DOI: doi:10.1038/s41586-020-2829-0 Pubmed: 33087931 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Megabody-25 (Complex from Lama glama)
- Human gaba(a)r-beta3 homopentamer (Complex from Homo sapiens)
- Histamine (111 Da, Ligand)
- Hexadecane (226 Da, Ligand)
- Gamma-aminobutyric acid receptor subunit beta-3,gamma-aminobutyric acid receptor subunit beta-3 (45 kDa, Protein from Homo sapiens)
- Decane (142 Da, Ligand)
- N-octane (114 Da, Ligand)
- Human gaba(a)r-beta3 homopentamer in complex with megabody-25 in lipid nanodisc (485 kDa, Complex)
- Megabody mb25 (56 kDa, Protein from Lama glama)
- Water (18 Da, Ligand)
- Chloride ion (35 Da, Ligand)