CryoEM structure of human full-length alpha1beta3gamma2L GABA(A)R in complex with GABA and puerarin
Resolution: 2.4 Å
EM Method: Single-particle
Fitted PDBs: 9eqg
Q-score: 0.627
Lyu Q, Xue W, Liu R, Ma Q, Kasaragod VB, Sun S, Li Q, Chen Y, Yuan M, Yang Y, Zhang B, Nie A, Jia S, Shen C, Gao P, Rong W, Yu C, Bi Y, Zhang C, Nan F, Ning G, Rao Z, Yang X, Wang J, Wang W
Nature (2024) 634 pp. 936-943 [ Pubmed: 39261733 DOI: doi:10.1038/s41586-024-07929-5 ]
- Gamma-aminobutyric acid receptor subunit beta-3 (54 kDa, Protein from Homo sapiens)
- Hexane (86 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- 1,2-dilauroyl-sn-glycero-3-phosphate (535 Da, Ligand)
- Water (18 Da, Ligand)
- Palmitic acid (256 Da, Ligand)
- Gamma-amino-butanoic acid (103 Da, Ligand)
- Gamma-aminobutyric acid receptor subunit gamma-2 (56 kDa, Protein from Homo sapiens)
- [(2r)-1-octadecanoyloxy-3-[oxidanyl-[(1r,2r,3s,4r,5r,6s)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phospho ryl]oxy-propan-2-yl] (8z)-icosa-5,8,11,14-tetraenoate (1 kDa, Ligand)
- Hexadecane (226 Da, Ligand)
- Decane (142 Da, Ligand)
- (1r)-2-{[(s)-{[(2s)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9z)-octadec-9-enoate (749 Da, Ligand)
- Puerarin (416 Da, Ligand)
- Cryoem structure of human full-length alpha1beta3gamma2l gaba(a)r in complex with gaba and puerarin (250 kDa, Complex from Homo sapiens)
- Chloride ion (35 Da, Ligand)
- Gamma-aminobutyric acid receptor subunit alpha-1 (52 kDa, Protein from Homo sapiens)
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 7ldd
Q-score: 0.25
Yu J, Rao P, Clark S, Mitra J, Ha T, Gouaux E
Nature (2021) 594 pp. 448-453 [ DOI: doi:10.1038/s41586-021-03540-0 Pubmed: 33981040 ]
- Glutamate receptor (98 kDa, Protein from Mus musculus)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Glua1/a2-symmetric-conformation bound to antibody fragments (Complex)
- Hexadecane (226 Da, Ligand)
- Nonane (128 Da, Ligand)
- 6-[2-chloro-6-(trifluoromethoxy)phenyl]-1h-benzimidazol-2-ol (328 Da, Ligand)
- 15f1 fab light chain (25 kDa, Protein from Mus musculus)
- 11b8 scfv (Complex from Mus musculus)
- Protein cornichon homolog 2 (18 kDa, Protein from Mus musculus)
- N-octane (114 Da, Ligand)
- 11b8 scfv (27 kDa, Protein from Mus musculus)
- 15f1 fab heavy chain (27 kDa, Protein from Mus musculus)
- Glua1/a2-symmetric-conformation (Complex from Mus musculus)
- Decane (142 Da, Ligand)
- Voltage-dependent calcium channel gamma-8 subunit (43 kDa, Protein from Mus musculus)
- Dodecane (170 Da, Ligand)
- Tetradecane (198 Da, Ligand)
- {[7-morpholin-4-yl-2,3-dioxo-6-(trifluoromethyl)-3,4-dihydroquinoxalin-1(2h)-yl]methyl}phosphonic acid (409 Da, Ligand)
- 15f1 fab (Complex from Mus musculus)
- Heptane (100 Da, Ligand)
- Glutamate receptor 1 (101 kDa, Protein from Mus musculus)
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 7lde
Q-score: 0.221
Yu J, Rao P, Clark S, Mitra J, Ha T, Gouaux E
Nature (2021) 594 pp. 448-453 [ DOI: doi:10.1038/s41586-021-03540-0 Pubmed: 33981040 ]
- Glutamate receptor (98 kDa, Protein from Mus musculus)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Hexadecane (226 Da, Ligand)
- Nonane (128 Da, Ligand)
- 6-[2-chloro-6-(trifluoromethoxy)phenyl]-1h-benzimidazol-2-ol (328 Da, Ligand)
- 15f1 fab light chain (25 kDa, Protein from Mus musculus)
- 11b8 scfv (Complex from Mus musculus)
- Glua1/a2-asymmetric-conformation bound to antibody fragments (Complex)
- Protein cornichon homolog 2 (18 kDa, Protein from Mus musculus)
- Glua1/a2-asymmetric-conformation (Complex from Mus musculus)
- N-octane (114 Da, Ligand)
- 11b8 scfv (27 kDa, Protein from Mus musculus)
- 15f1 fab heavy chain (27 kDa, Protein from Mus musculus)
- Decane (142 Da, Ligand)
- Voltage-dependent calcium channel gamma-8 subunit (43 kDa, Protein from Mus musculus)
- Dodecane (170 Da, Ligand)
- Tetradecane (198 Da, Ligand)
- {[7-morpholin-4-yl-2,3-dioxo-6-(trifluoromethyl)-3,4-dihydroquinoxalin-1(2h)-yl]methyl}phosphonic acid (409 Da, Ligand)
- 15f1 fab (Complex from Mus musculus)
- Heptane (100 Da, Ligand)
- Glutamate receptor 1 (101 kDa, Protein from Mus musculus)
Resolution: 3.25 Å
EM Method: Single-particle
Fitted PDBs: 7lep
Q-score: 0.498
Yu J, Rao P, Clark S, Mitra J, Ha T, Gouaux E
Nature (2021) 594 pp. 448-453 [ DOI: doi:10.1038/s41586-021-03540-0 Pubmed: 33981040 ]
- Tetradecane (198 Da, Ligand)
- {[7-morpholin-4-yl-2,3-dioxo-6-(trifluoromethyl)-3,4-dihydroquinoxalin-1(2h)-yl]methyl}phosphonic acid (409 Da, Ligand)
- Protein cornichon homolog 2 (18 kDa, Protein from Mus musculus)
- Glutamate receptor 2 (47 kDa, Protein from Mus musculus)
- Mix of ampar subunits (glua1, glua3, and glua4) (43 kDa, Protein from Mus musculus)
- Mix of ampar subunits (glua1, glua3, and gluax) (43 kDa, Protein from Mus musculus)
- Composite lbd-tmd complex from the combined hippocampal ampar subtypes (Complex from Mus musculus)
- Dodecane (170 Da, Ligand)
- Decane (142 Da, Ligand)
- Voltage-dependent calcium channel gamma-8 subunit (23 kDa, Protein from Mus musculus)
- 6-[2-chloro-6-(trifluoromethoxy)phenyl]-1h-benzimidazol-2-ol (328 Da, Ligand)
- N-octane (114 Da, Ligand)
- (2s)-3-(hexadecanoyloxy)-2-[(9z)-octadec-9-enoyloxy]propyl 2-(trimethylammonio)ethyl phosphate (760 Da, Ligand)