Resolution: 2.5 Å
EM Method: Single-particle
Fitted PDBs: 7qn5
Q-score: 0.58
Sente A, Desai R, Naydenova K, Malinauskas T, Jounaidi Y, Miehling J, Zhou X, Masiulis S, Hardwick SW, Chirgadze DY, Miller KW, Aricescu AR
Nature (2022) 604 pp. 190-194 [ Pubmed: 35355020 DOI: doi:10.1038/s41586-022-04517-3 ]
- 1,2-dipalmitoyl-sn-glycero-3-phosphate (647 Da, Ligand)
- Human full-length extrasynaptic alpha4beta3delta gaba(a)r in complex with nanobody nb25 (Complex from Homo sapiens)
- Water (18 Da, Ligand)
- Gamma-aminobutyric acid receptor subunit alpha-4 (61 kDa, Protein from Homo sapiens)
- N-octane (114 Da, Ligand)
- Nanobody nb25 (13 kDa, Protein from Lama glama)
- Chloride ion (35 Da, Ligand)
- Decane (142 Da, Ligand)
- 4-(2-hydroxyethyl)-1-piperazine ethanesulfonic acid (238 Da, Ligand)
- Gamma-aminobutyric acid receptor subunit beta-3 (54 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Gamma-aminobutyric acid receptor subunit delta (52 kDa, Protein from Homo sapiens)
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7qn7
Q-score: 0.548
Sente A, Desai R, Naydenova K, Malinauskas T, Jounaidi Y, Miehling J, Zhou X, Masiulis S, Hardwick SW, Chirgadze DY, Miller KW, Aricescu AR
Nature (2022) 604 pp. 190-194 [ Pubmed: 35355020 DOI: doi:10.1038/s41586-022-04517-3 ]
- 1,2-dipalmitoyl-sn-glycero-3-phosphate (647 Da, Ligand)
- Hexadecane (226 Da, Ligand)
- Water (18 Da, Ligand)
- Nanobody nb25 (13 kDa, Protein from Homo sapiens)
- Gamma-aminobutyric acid receptor subunit alpha-4 (61 kDa, Protein from Homo sapiens)
- Human full-length extrasynaptic alpha4beta3delta gaba(a)r in complex with gaba, histamine and nanobody nb25 (Complex from Homo sapiens)
- Histamine (111 Da, Ligand)
- Gamma-amino-butanoic acid (103 Da, Ligand)
- Decane (142 Da, Ligand)
- Gamma-aminobutyric acid receptor subunit beta-3 (54 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Gamma-aminobutyric acid receptor subunit delta (52 kDa, Protein from Homo sapiens)
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7qn9
Q-score: 0.505
Sente A, Desai R, Naydenova K, Malinauskas T, Jounaidi Y, Miehling J, Zhou X, Masiulis S, Hardwick SW, Chirgadze DY, Miller KW, Aricescu AR
Nature (2022) 604 pp. 190-194 [ Pubmed: 35355020 DOI: doi:10.1038/s41586-022-04517-3 ]
- Hexadecane (226 Da, Ligand)
- Nanobody nb25 (13 kDa, Protein from Homo sapiens)
- Gamma-aminobutyric acid receptor subunit alpha-4 (61 kDa, Protein from Homo sapiens)
- Histamine (111 Da, Ligand)
- Decane (142 Da, Ligand)
- Gamma-aminobutyric acid receptor subunit beta-3 (54 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Human full-length extrasynaptic alpha4beta3delta gaba(a)r in complex with histamine and nanobody nb25 (Complex from Homo sapiens)
- Gamma-aminobutyric acid receptor subunit delta (52 kDa, Protein from Homo sapiens)
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7qnc
Q-score: 0.523
Sente A, Desai R, Naydenova K, Malinauskas T, Jounaidi Y, Miehling J, Zhou X, Masiulis S, Hardwick SW, Chirgadze DY, Miller KW, Aricescu AR
Nature (2022) 604 pp. 190-194 [ Pubmed: 35355020 DOI: doi:10.1038/s41586-022-04517-3 ]
- Human full-length extrasynaptic alpha4beta3delta gaba(a)r in complex with thip (gaboxadol), histamine and nanobody nb25 (Complex from Homo sapiens)
- Gamma-aminobutyric acid receptor subunit alpha-4 (61 kDa, Protein from Homo sapiens)
- Nanobody nb25 (13 kDa, Protein from Lama glama)
- Histamine (111 Da, Ligand)
- Decane (142 Da, Ligand)
- 4,5,6,7-tetrahydro-[1,2]oxazolo[5,4-c]pyridin-3-one (140 Da, Ligand)
- Gamma-aminobutyric acid receptor subunit beta-3 (54 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Gamma-aminobutyric acid receptor subunit delta (52 kDa, Protein from Homo sapiens)
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 7qne
Q-score: 0.606
Sente A, Desai R, Naydenova K, Malinauskas T, Jounaidi Y, Miehling J, Zhou X, Masiulis S, Hardwick SW, Chirgadze DY, Miller KW, Aricescu AR
Nature (2022) 604 pp. 190-194 [ Pubmed: 35355020 DOI: doi:10.1038/s41586-022-04517-3 ]
- Human full-length synaptic alpha1beta3gamma2 gaba(a)r in complex with ro15-4513 and megabody mb38 (Complex from Homo sapiens)
- Megabody mb38 (13 kDa, Protein from Lama glama)
- Gaba(a) receptor subunit alpha-1 (51 kDa, Protein from Homo sapiens)
- N-octane (114 Da, Ligand)
- Chloride ion (35 Da, Ligand)
- Gaba(a) receptor subunit gamma-2 (56 kDa, Protein from Homo sapiens)
- Decane (142 Da, Ligand)
- Gamma-aminobutyric acid receptor subunit beta-3 (54 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- [(2r)-2-octanoyloxy-3-[oxidanyl-[(1r,2r,3s,4r,5r,6s)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate (746 Da, Ligand)
- Ethyl 8-[(azanylidene-$l^{4}-azanylidene)amino]-5-methyl-6-oxidanylidene-4~{h}-imidazo[1,5-a][1,4]benzodiazepine-3-carboxylate (326 Da, Ligand)
Resolution: 2.79 Å
EM Method: Single-particle
Fitted PDBs: 7pbz
Q-score: 0.548
Kasaragod VB, Mortensen M, Hardwick SW, Wahid AA, Dorovykh V, Chirgadze DY, Smart TG, Miller PS
Nature (2022) 602 pp. 529-533 [ Pubmed: 35140402 DOI: doi:10.1038/s41586-022-04402-z ]
- Hexane (86 Da, Ligand)
- Gaba-a receptor - megabody 25 complex (220 kDa, Complex)
- Gamma-aminobutyric acid receptor subunit alpha-1 (41 kDa, Protein from Homo sapiens)
- Megabody 25 (56 kDa, Protein from Lama glama)
- N-octane (114 Da, Ligand)
- Gamma-aminobutyric acid receptor subunit beta-3 (51 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Histamine (111 Da, Ligand)
- Gamma-amino-butanoic acid (103 Da, Ligand)
- Decane (142 Da, Ligand)
- Gaba-a receptor (Complex from Homo sapiens)
- Undecane (156 Da, Ligand)
- Gamma-aminobutyric acid receptor subunit alpha-1 (47 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Megabody 25 (Complex from Lama glama)
Resolution: 1.7 Å
EM Method: Single-particle
Fitted PDBs: 7a5v
Q-score: 0.75
Nakane T, Kotecha A, Sente A, McMullan G, Masiulis S, Brown PMGE, Grigoras IT, Malinauskaite L, Malinauskas T, Miehling J, Uchanski T, Yu L, Karia D, Pechnikova EV, de Jong E, Keizer J, Bischoff M, McCormack J, Tiemeijer P, Hardwick SW, Chirgadze DY, Murshudov G, Aricescu AR, Scheres SHW
Nature (2020) 587 pp. 152-156 [ DOI: doi:10.1038/s41586-020-2829-0 Pubmed: 33087931 ]
- Hexadecane (226 Da, Ligand)
- Water (18 Da, Ligand)
- Human gaba(a)r-beta3 homopentamer (Complex from Homo sapiens)
- Megabody-25 (Complex from Lama glama)
- N-octane (114 Da, Ligand)
- Human gaba(a)r-beta3 homopentamer in complex with megabody-25 in lipid nanodisc (485 kDa, Complex)
- Histamine (111 Da, Ligand)
- Chloride ion (35 Da, Ligand)
- Decane (142 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Megabody mb25 (56 kDa, Protein from Lama glama)
- Gamma-aminobutyric acid receptor subunit beta-3,gamma-aminobutyric acid receptor subunit beta-3 (45 kDa, Protein from Homo sapiens)