Structure of GABAAR determined by cryoEM at 100 keV
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 8pvb
Q-score: 0.486
McMullan G, Naydenova K, Mihaylov D, Yamashita K, Peet MJ, Wilson H, Dickerson JL, Chen S, Cannone G, Lee Y, Hutchings KA, Gittins O, Sobhy MA, Wells T, El-Gomati MM, Dalby J, Meffert M, Schulze-Briese C, Henderson R, Russo CJ
PNAS (2023) 120 pp. e2312905120-e2312905120 [ Pubmed: 38011573 DOI: doi:10.1073/pnas.2312905120 ]
- Human gaba(a)r-beta3 homopentamer in complex with megabody-25 in lipid nanodisc (Complex from Homo sapiens)
- Histamine (111 Da, Ligand)
- Hexadecane (226 Da, Ligand)
- Decane (142 Da, Ligand)
- Gamma-aminobutyric acid receptor subunit beta-3 (45 kDa, Protein from Homo sapiens)
- Megabody mb25 (56 kDa, Protein from Lama glama)
- Etomidate (244 Da, Ligand)
- Chloride ion (35 Da, Ligand)
Transport protein in an occluded state
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7m5v
Q-score: 0.49
Tillinghast J, Drury S, Bowser D, Benn A, Lee KPK
Mol Cell (2021) 81 pp. 4650-4662.e4 [ Pubmed: 34715014 DOI: doi:10.1016/j.molcel.2021.10.002 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Water (18 Da, Ligand)
- Polyamine-transporting atpase 13a2 (129 kDa, Protein from Homo sapiens)
- (2r)-3-{[(s)-hydroxy{[(1s,2r,3r,4s,5s,6r)-2,4,6-trihydroxy-3,5-bis(phosphonooxy)cyclohexyl]oxy}phosphoryl]oxy}propane-1,2-diyl dioctanoate (746 Da, Ligand)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Decane (142 Da, Ligand)
- Transport protein in an occluded state (130 kDa, Complex from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Cholesterol hemisuccinate (486 Da, Ligand)
- Dodecyl-beta-d-maltoside (510 Da, Ligand)
- 1-dodecanol (186 Da, Ligand)
- Dodecane (170 Da, Ligand)
Transport protein in inhibited state 1
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 7m5x
Q-score: 0.484
Tillinghast J, Drury S, Bowser D, Benn A, Lee KPK
Mol Cell (2021) 81 pp. 4650-4662.e4 [ Pubmed: 34715014 DOI: doi:10.1016/j.molcel.2021.10.002 ]
- Tetradecane (198 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- (2r)-3-{[(s)-hydroxy{[(1s,2r,3r,4s,5s,6r)-2,4,6-trihydroxy-3,5-bis(phosphonooxy)cyclohexyl]oxy}phosphoryl]oxy}propane-1,2-diyl dioctanoate (746 Da, Ligand)
- Spermine (fully protonated form) (206 Da, Ligand)
- Dodecane (170 Da, Ligand)
- Polyamine-transporting atpase 13a2 (129 kDa, Protein from Homo sapiens)
- Decane (142 Da, Ligand)
- Transport protein in inhibited state 1 (130 kDa, Complex from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Beryllium trifluoride ion (66 Da, Ligand)
- Cholesterol hemisuccinate (486 Da, Ligand)
- Dodecyl-beta-d-maltoside (510 Da, Ligand)
- Water (18 Da, Ligand)
- Cholesterol (386 Da, Ligand)
Transport protein in inhibited state 2
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7m5y
Q-score: 0.452
Tillinghast J, Drury S, Bowser D, Benn A, Lee KPK
Mol Cell (2021) 81 pp. 4650-4662.e4 [ Pubmed: 34715014 DOI: doi:10.1016/j.molcel.2021.10.002 ]
- Tetradecane (198 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- (2r)-3-{[(s)-hydroxy{[(1s,2r,3r,4s,5s,6r)-2,4,6-trihydroxy-3,5-bis(phosphonooxy)cyclohexyl]oxy}phosphoryl]oxy}propane-1,2-diyl dioctanoate (746 Da, Ligand)
- Spermine (fully protonated form) (206 Da, Ligand)
- Transport protein in inhibited state 2 monomer (130 kDa, Complex from Homo sapiens)
- Dodecane (170 Da, Ligand)
- Polyamine-transporting atpase 13a2 (129 kDa, Protein from Homo sapiens)
- Decane (142 Da, Ligand)
- Tetrafluoromagnesate(2-) (100 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Cholesterol hemisuccinate (486 Da, Ligand)
- Dodecyl-beta-d-maltoside (510 Da, Ligand)
- Water (18 Da, Ligand)
- Cholesterol (386 Da, Ligand)
afTMEM16 reconstituted in nanodiscs in the presence of Ca2+ and ceramide 24:0
Resolution: 3.59 Å
EM Method: Single-particle
Fitted PDBs: 6e1o
Q-score: 0.471
Falzone ME, Rheinberger J, Lee BC, Peyear T, Sasset L, Raczkowski AM, Eng ET, Di Lorenzo A, Andersen OS, Nimigean CM, Accardi A
eLife (2019) 8 [ Pubmed: 30648972 DOI: doi:10.7554/eLife.43229 ]
- Hexane (86 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- Octadecane (254 Da, Ligand)
- Aftmem16 reconstituted in nanodiscs in the presence of ca2+ and ceramide 24:0 (168 MDa, Complex from Aspergillus fumigatus)
- Plasma membrane channel protein (aqy1), putative (84 kDa, Protein from Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100))
- Decane (142 Da, Ligand)
- Dodecane (170 Da, Ligand)