D. melanogaster CMG-DNA with ATP, State 2B
Resolution: 4.46 Å
EM Method: Single-particle
Fitted PDBs: 6raz
Q-score: 0.246
Eickhoff P, Kose HB, Martino F, Petojevic T, Abid Ali F, Locke J, Tamberg N, Nans A, Berger JM, Botchan MR, Yardimci H, Costa A
Cell Rep (2019) 28 pp. 2673-2688.e8 [ DOI: doi:10.1016/j.celrep.2019.07.104 Pubmed: 31484077 ]
- Cdc45l (65 kDa, Protein from Drosophila melanogaster)
- Dna replication licensing factor mcm3 (91 kDa, Protein from Drosophila melanogaster)
- Dna replication licensing factor mcm7 (81 kDa, Protein from Drosophila melanogaster)
- Dna (6 kDa, DNA from Drosophila melanogaster)
- Dna replication licensing factor mcm5 (82 kDa, Protein from Drosophila melanogaster)
- Cmg helicase bound to forked dna in the presence of atp, state 2b (Complex)
- Dna replication licensing factor mcm6 (92 kDa, Protein from Drosophila melanogaster)
- Dna replication licensing factor mcm4 (96 kDa, Protein from Drosophila melanogaster)
- Dna replication complex gins protein sld5 (26 kDa, Protein from Drosophila melanogaster)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Dna (573 Da, DNA from Drosophila melanogaster)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Dna (Complex from Drosophila melanogaster)
- At18545p (24 kDa, Protein from Drosophila melanogaster)
- Dna replication licensing factor mcm2 (100 kDa, Protein from Drosophila melanogaster)
- Cmg helicase (Complex from Drosophila melanogaster)
- Ip07275p (23 kDa, Protein from Drosophila melanogaster)
- Probable dna replication complex gins protein psf2 (23 kDa, Protein from Drosophila melanogaster)
D. melanogaster CMG-DNA with ATP, State 1A
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 6raw
Q-score: 0.215
Eickhoff P, Kose HB, Martino F, Petojevic T, Abid Ali F, Locke J, Tamberg N, Nans A, Berger JM, Botchan MR, Yardimci H, Costa A
Cell Rep (2019) 28 pp. 2673-2688.e8 [ DOI: doi:10.1016/j.celrep.2019.07.104 Pubmed: 31484077 ]
- Cdc45l (65 kDa, Protein from Drosophila melanogaster)
- Cmg helicase bound to forked dna in the presence of atp, state 1a (Complex)
- Dna replication licensing factor mcm3 (91 kDa, Protein from Drosophila melanogaster)
- Dna replication licensing factor mcm7 (81 kDa, Protein from Drosophila melanogaster)
- Dna replication licensing factor mcm4 (96 kDa, Protein from Drosophila melanogaster)
- Dna replication licensing factor mcm5 (82 kDa, Protein from Drosophila melanogaster)
- Dna replication licensing factor mcm6 (92 kDa, Protein from Drosophila melanogaster)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Dna replication complex gins protein sld5 (26 kDa, Protein from Drosophila melanogaster)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Dna (Complex from Drosophila melanogaster)
- Dna replication licensing factor mcm2 (100 kDa, Protein from Drosophila melanogaster)
- Probable dna replication complex gins protein psf2 (23 kDa, Protein from Drosophila melanogaster)
- At18545p (24 kDa, Protein from Drosophila melanogaster)
- Dna (5'-d(p*tp*cp*gp*ap*tp*cp*gp*ap*tp*cp*gp*ap*t)-3') (3 kDa, DNA from Drosophila melanogaster)
- Cmg helicase (Complex from Drosophila melanogaster)
- Ip07275p (23 kDa, Protein from Drosophila melanogaster)
- Dna (7 kDa, DNA from Drosophila melanogaster)
D. melanogaster CMG-DNA with ATP, State 1B
Resolution: 3.99 Å
EM Method: Single-particle
Fitted PDBs: 6rax
Q-score: 0.255
Eickhoff P, Kose HB, Martino F, Petojevic T, Abid Ali F, Locke J, Tamberg N, Nans A, Berger JM, Botchan MR, Yardimci H, Costa A
Cell Rep (2019) 28 pp. 2673-2688.e8 [ DOI: doi:10.1016/j.celrep.2019.07.104 Pubmed: 31484077 ]
- Cdc45l (65 kDa, Protein from Drosophila melanogaster)
- Dna replication licensing factor mcm3 (91 kDa, Protein from Drosophila melanogaster)
- Dna replication licensing factor mcm7 (81 kDa, Protein from Drosophila melanogaster)
- Dna replication licensing factor mcm4 (96 kDa, Protein from Drosophila melanogaster)
- Dna replication licensing factor mcm5 (82 kDa, Protein from Drosophila melanogaster)
- Cmg helicase bound to forked dna in the presence of atp, state 1b (Complex)
- Dna replication licensing factor mcm6 (92 kDa, Protein from Drosophila melanogaster)
- Dna (2 kDa, DNA from Drosophila melanogaster)
- Dna replication complex gins protein sld5 (26 kDa, Protein from Drosophila melanogaster)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Dna (Complex from Drosophila melanogaster)
- Dna replication licensing factor mcm2 (100 kDa, Protein from Drosophila melanogaster)
- Probable dna replication complex gins protein psf2 (23 kDa, Protein from Drosophila melanogaster)
- At18545p (24 kDa, Protein from Drosophila melanogaster)
- Cmg helicase (Complex from Drosophila melanogaster)
- Ip07275p (23 kDa, Protein from Drosophila melanogaster)
- Dna (6 kDa, DNA from Drosophila melanogaster)
D. melanogaster CMG-DNA with ATP, State 2A
Resolution: 4.28 Å
EM Method: Single-particle
Fitted PDBs: 6ray
Q-score: 0.235
Eickhoff P, Kose HB, Martino F, Petojevic T, Abid Ali F, Locke J, Tamberg N, Nans A, Berger JM, Botchan MR, Yardimci H, Costa A
Cell Rep (2019) 28 pp. 2673-2688.e8 [ DOI: doi:10.1016/j.celrep.2019.07.104 Pubmed: 31484077 ]
- Cdc45l (65 kDa, Protein from Drosophila melanogaster)
- Dna replication licensing factor mcm3 (91 kDa, Protein from Drosophila melanogaster)
- Cmg helicase bound to forked dna in the presence of atp, state 2a (Complex)
- Dna replication licensing factor mcm4 (96 kDa, Protein from Drosophila melanogaster)
- Dna replication licensing factor mcm7 (81 kDa, Protein from Drosophila melanogaster)
- Dna replication licensing factor mcm6 (92 kDa, Protein from Drosophila melanogaster)
- Dna replication licensing factor mcm5 (82 kDa, Protein from Drosophila melanogaster)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Dna replication complex gins protein sld5 (26 kDa, Protein from Drosophila melanogaster)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Dna (Complex from Drosophila melanogaster)
- Dna replication licensing factor mcm2 (100 kDa, Protein from Drosophila melanogaster)
- Probable dna replication complex gins protein psf2 (23 kDa, Protein from Drosophila melanogaster)
- At18545p (24 kDa, Protein from Drosophila melanogaster)
- Dna (5'-d(p*ap*tp*tp*tp*tp*tp*tp*tp*tp*tp*tp*tp*t)-3') (3 kDa, DNA from Drosophila melanogaster)
- Cmg helicase (Complex from Drosophila melanogaster)
- Ip07275p (23 kDa, Protein from Drosophila melanogaster)
S. cerevisiae CMG-Pol epsilon-DNA
Resolution: 4.98 Å
EM Method: Single-particle
Fitted PDBs: 6hv9
Q-score: 0.231
Goswami P, Abid Ali F, Douglas ME, Locke J, Purkiss A, Janska A, Eickhoff P, Early A, Nans A, Cheung AMC, Diffley JFX, Costa A
Nat Commun (2018) 9 pp. 5061-5061 [ Pubmed: 30498216 DOI: doi:10.1038/s41467-018-07417-1 ]
- Dna polymerase epsilon catalytic subunit (104 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm3 (107 kDa, Protein from Saccharomyces cerevisiae)
- Zinc ion (65 Da, Ligand)
- Dna replication licensing factor mcm2 (98 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm6 (113 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication complex gins protein psf1 (24 kDa, Protein from Saccharomyces cerevisiae)
- Cmg-pol epsilon (Complex from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm5 (86 kDa, Protein from Saccharomyces cerevisiae)
- Dna (5'-d(p*tp*tp*tp*tp*tp*tp*t)-3') (2 kDa, DNA from Saccharomyces cerevisiae)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Nucleic acid (Complex from Saccharomyces cerevisiae)
- Dna replication complex gins protein sld5 (33 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication complex gins protein psf3 (21 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm7 (95 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication complex gins protein psf2 (25 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm4 (105 kDa, Protein from Saccharomyces cerevisiae)
- Dna polymerase epsilon subunit b (78 kDa, Protein from Saccharomyces cerevisiae)
- Cell division control protein 45 (74 kDa, Protein from Saccharomyces cerevisiae)
- Dna (5'-d(p*tp*ap*ap*ap*ap*cp*gp*gp*cp*cp*ap*gp*cp*gp*tp*gp*gp*cp*tp*gp*c)-3') (6 kDa, DNA from Saccharomyces cerevisiae)
- Dna (5'-d(*gp*cp*ap*gp*cp*cp*ap*cp*gp*cp*tp*gp*gp*cp*cp*gp*tp*tp*tp*tp*a)-3') (6 kDa, DNA from Saccharomyces cerevisiae)
- Cmg-pol epsilon-dna (Complex)