Human OCCM DNA licensing intermediate
Resolution: 6.68 Å
EM Method: Single-particle
Fitted PDBs: 8rwv
Q-score: 0.186
Wells JN, Edwardes LV, Leber V, Allyjaun S, Peach M, Tomkins J, Kefala-Stavridi A, Faull SV, Aramayo R, Pestana CM, Ranjha L, Speck C
Nat Commun (2025) 16 pp. 478-478 [ DOI: doi:10.1038/s41467-024-55772-z Pubmed: 39779677 ]
- Origin recognition complex subunit 2 (66 kDa, Protein from Homo sapiens)
- Dna replication licensing factor mcm2 (106 kDa, Protein from Homo sapiens)
- Isoform 2 of dna replication licensing factor mcm3 (96 kDa, Protein from Homo sapiens)
- Dna replication factor cdt1 (46 kDa, Protein from Homo sapiens)
- Dna replication licensing factor mcm4 (99 kDa, Protein from Homo sapiens)
- Dna replication licensing factor mcm7 (81 kDa, Protein from Homo sapiens)
- Isoform 2 of origin recognition complex subunit 3 (82 kDa, Protein from Homo sapiens)
- Dna replication licensing factor mcm6 (93 kDa, Protein from Homo sapiens)
- Origin recognition complex subunit 4 (50 kDa, Protein from Homo sapiens)
- Dna (11 kDa, DNA from Homo sapiens)
- Origin recognition complex subunit 1 (108 kDa, Protein from Homo sapiens)
- Cell division control protein 6 homolog (62 kDa, Protein from Homo sapiens)
- Dna (12 kDa, DNA from Homo sapiens)
- Hoccm (1 MDa, Complex from Homo sapiens)
- Origin recognition complex subunit 5 (50 kDa, Protein from Homo sapiens)
Cryo-EM structure of the MCM double hexamer loaded onto dsDNA.
Resolution: 2.79 Å
EM Method: Single-particle
Fitted PDBs: 8rif
Q-score: 0.516
Miller TCR, Lim CT, Diffley JFX, Costa A
To Be Published
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Dna replication licensing factor mcm4 (105 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm7 (95 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm6 (113 kDa, Protein from Saccharomyces cerevisiae)
- Minichromosome maintenance protein 5 (86 kDa, Protein from Saccharomyces cerevisiae)
- Mcm-double hexamer loaded onto double-stranded dna (1 MDa, Complex from Saccharomyces cerevisiae)
- Dna (53-mer) (16 kDa, DNA from Saccharomyces cerevisiae)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Dna replication licensing factor mcm3 (111 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm2 (98 kDa, Protein from Saccharomyces cerevisiae)
- Dna (53-mer) (16 kDa, DNA from Saccharomyces cerevisiae)
Cryo-EM structure of an MCM helicase single hexamer loaded onto dsDNA.
Resolution: 3.41 Å
EM Method: Single-particle
Fitted PDBs: 8rig
Q-score: 0.44
Miller TCR, Lim CT, Diffley JFX, Costa A
To Be Published
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Dna (5'-d(p*ap*tp*gp*cp*ap*tp*gp*cp*ap*tp*gp*cp*ap*tp*gp*cp*ap*t)-3') (5 kDa, DNA from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm4 (105 kDa, Protein from Saccharomyces cerevisiae S288C)
- Hexameric mcm helicase complex loaded onto duplex dna (610 kDa, Complex from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm3 (111 kDa, Protein from Saccharomyces cerevisiae S288C)
- Minichromosome maintenance protein 5 (86 kDa, Protein from Saccharomyces cerevisiae S288C)
- Dna (5'-d(p*gp*cp*ap*tp*gp*cp*ap*tp*gp*cp*ap*tp*gp*cp*ap*tp*gp*cp*ap*t)-3') (5 kDa, DNA from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm2 (98 kDa, Protein from Saccharomyces cerevisiae S288C)
- Dna replication licensing factor mcm6 (113 kDa, Protein from Saccharomyces cerevisiae S288C)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Dna replication licensing factor mcm7 (95 kDa, Protein from Saccharomyces cerevisiae S288C)
Resolution: 6.1 Å
EM Method: Single-particle
Fitted PDBs: 9bcx
Q-score: 0.133
Faull SV, Barbon M, Mossler A, Yuan Z, Bai L, Reuter LM, Riera A, Winkler C, Magdalou I, Peach M, Li H, Speck C
Nat Commun (2025) 16 pp. 14-14 [ Pubmed: 39747125 DOI: doi:10.1038/s41467-024-55479-1 ]
- Dna replication licensing factor mcm4 (105 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm6 (113 kDa, Protein from Saccharomyces cerevisiae)
- Origin recognition complex subunit 5 (55 kDa, Protein from Saccharomyces cerevisiae)
- Origin recognition complex subunit 3 (72 kDa, Protein from Saccharomyces cerevisiae)
- Origin recognition complex subunit 2 (71 kDa, Protein from Saccharomyces cerevisiae)
- Cell division control protein 6 (58 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm5 (86 kDa, Protein from Saccharomyces cerevisiae)
- Tah11 isoform 1 (68 kDa, Protein from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm7 (95 kDa, Protein from Saccharomyces cerevisiae)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Origin recognition complex subunit 1 (104 kDa, Protein from Saccharomyces cerevisiae)
- Origin recognition complex subunit 4 (60 kDa, Protein from Saccharomyces cerevisiae)
- Dna (39-mer) (12 kDa, DNA from synthetic construct)
- Dna replication licensing factor mcm3 (107 kDa, Protein from Saccharomyces cerevisiae)
- Magnesium ion (24 Da, Ligand)
- Dna (39-mer) (11 kDa, DNA from synthetic construct)
- Origin recognition complex subunit 6 (50 kDa, Protein from Saccharomyces cerevisiae)
- Orc-cdc6-mcm2-7-dsdna (Complex from Saccharomyces cerevisiae)
- Dna replication licensing factor mcm2 (98 kDa, Protein from Saccharomyces cerevisiae)
H. sapiens MCM2-7 double hexamer bound to double stranded DNA
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 8s09
Q-score: 0.448
Weissmann F, Greiwe JF, Puhringer T, Eastwood EL, Couves EC, Miller TCR, Diffley JFX, Costa A
Nature (2024) 636 pp. 499-508 [ DOI: doi:10.1038/s41586-024-08263-6 Pubmed: 39604733 ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Dna replication licensing factor mcm4 (96 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Dna replication licensing factor mcm2 (102 kDa, Protein from Homo sapiens)
- Dna replication licensing factor mcm7 (81 kDa, Protein from Homo sapiens)
- Dna (45-mer) (13 kDa, DNA from synthetic construct)
- Dna replication licensing factor mcm6 (93 kDa, Protein from Homo sapiens)
- Dna replication licensing factor mcm3 (91 kDa, Protein from Homo sapiens)
- Dna replication licensing factor mcm5 (82 kDa, Protein from Homo sapiens)
- H. sapiens mcm2-7 double hexamer bound to double stranded dna (1 MDa, Complex)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Dna (45-mer) (13 kDa, DNA from synthetic construct)
- H. sapiens mcm2-7 (Complex from Homo sapiens)
- Double stranded dna (Complex from synthetic construct)
H. sapiens MCM2-7 hexamer bound to double stranded DNA
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8s0a
Q-score: 0.442
Weissmann F, Greiwe JF, Puhringer T, Eastwood EL, Couves EC, Miller TCR, Diffley JFX, Costa A
Nature (2024) 636 pp. 499-508 [ DOI: doi:10.1038/s41586-024-08263-6 Pubmed: 39604733 ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Dna replication licensing factor mcm4 (96 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Dna (22-mer) (6 kDa, DNA from synthetic construct)
- Dna replication licensing factor mcm2 (102 kDa, Protein from Homo sapiens)
- Dna replication licensing factor mcm7 (81 kDa, Protein from Homo sapiens)
- Dna replication licensing factor mcm6 (93 kDa, Protein from Homo sapiens)
- Dna replication licensing factor mcm3 (91 kDa, Protein from Homo sapiens)
- Dna replication licensing factor mcm5 (82 kDa, Protein from Homo sapiens)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- H. sapiens mcm2-7 hexamer bound to double stranded dna (600 kDa, Complex)
- H. sapiens mcm2-7 (Complex from Homo sapiens)
- Double stranded dna (Complex from synthetic construct)
H. sapiens MCM bound to double stranded DNA and ORC6 as part of the MCM-ORC complex
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 8s0b
Q-score: 0.377
Weissmann F, Greiwe JF, Puhringer T, Eastwood EL, Couves EC, Miller TCR, Diffley JFX, Costa A
Nature (2024) 636 pp. 499-508 [ DOI: doi:10.1038/s41586-024-08263-6 Pubmed: 39604733 ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Origin recognition complex subunit 6 (28 kDa, Protein from Homo sapiens)
- Dna replication licensing factor mcm4 (96 kDa, Protein from Homo sapiens)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Dna (45-mer) (13 kDa, DNA from synthetic construct)
- Dna replication licensing factor mcm2 (102 kDa, Protein from Homo sapiens)
- Dna replication licensing factor mcm7 (81 kDa, Protein from Homo sapiens)
- Dna replication licensing factor mcm6 (93 kDa, Protein from Homo sapiens)
- H. sapiens orc6 (Complex from Homo sapiens)
- Dna replication licensing factor mcm3 (91 kDa, Protein from Homo sapiens)
- H. sapiens mcm bound to double stranded dna and orc6 as part of the mcm-orc complex (600 kDa, Complex)
- Dna replication licensing factor mcm5 (82 kDa, Protein from Homo sapiens)
- Dna (45-mer) (13 kDa, DNA from synthetic construct)
- Magnesium ion (24 Da, Ligand)
- H. sapiens mcm2-7 (Complex from Homo sapiens)
- Double stranded dna (Complex from synthetic construct)
H. sapiens MCM bound to double stranded DNA and ORC1-6
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 8s0d
Q-score: 0.346
Weissmann F, Greiwe JF, Puhringer T, Eastwood EL, Couves EC, Miller TCR, Diffley JFX, Costa A
Nature (2024) 636 pp. 499-508 [ DOI: doi:10.1038/s41586-024-08263-6 Pubmed: 39604733 ]
- H. sapiens mcm (Complex from Homo sapiens)
- Double stranded dna (Complex from synthetic construct)
- H. sapiens orc1-5 (Complex from Homo sapiens)
- Origin recognition complex subunit 2 (66 kDa, Protein from Homo sapiens)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Dna replication licensing factor mcm6 (93 kDa, Protein from Homo sapiens)
- Dna replication licensing factor mcm3 (91 kDa, Protein from Homo sapiens)
- Dna replication licensing factor mcm5 (82 kDa, Protein from Homo sapiens)
- Dna (58-mer) (17 kDa, DNA from synthetic construct)
- Isoform 2 of origin recognition complex subunit 3 (82 kDa, Protein from Homo sapiens)
- Origin recognition complex subunit 1 (97 kDa, Protein from Homo sapiens)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Dna replication licensing factor mcm7 (81 kDa, Protein from Homo sapiens)
- Origin recognition complex subunit 4 (50 kDa, Protein from Homo sapiens)
- H. sapiens orc6 (Complex from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Dna replication licensing factor mcm2 (101 kDa, Protein from Homo sapiens)
- Origin recognition complex subunit 5 (50 kDa, Protein from Homo sapiens)
- Origin recognition complex subunit 6 (28 kDa, Protein from Homo sapiens)
- Dna replication licensing factor mcm4 (96 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Dna (58-mer) (17 kDa, DNA from synthetic construct)
- H. sapiens mcm bound to double stranded dna and orc1-6 (1 MDa, Complex)
H. sapiens OCCM bound to double stranded DNA
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 8s0e
Q-score: 0.24
Weissmann F, Greiwe JF, Puhringer T, Eastwood EL, Couves EC, Miller TCR, Diffley JFX, Costa A
Nature (2024) 636 pp. 499-508 [ DOI: doi:10.1038/s41586-024-08263-6 Pubmed: 39604733 ]
- Cell division control protein 6 homolog (62 kDa, Protein from Homo sapiens)
- Dna replication licensing factor mcm2 (102 kDa, Protein from Homo sapiens)
- Double stranded dna (Complex from synthetic construct)
- Origin recognition complex subunit 2 (66 kDa, Protein from Homo sapiens)
- Dna replication licensing factor mcm6 (93 kDa, Protein from Homo sapiens)
- Dna replication licensing factor mcm3 (91 kDa, Protein from Homo sapiens)
- Dna replication licensing factor mcm5 (82 kDa, Protein from Homo sapiens)
- H. sapiens occm (Complex from Homo sapiens)
- Origin recognition complex subunit 1 (97 kDa, Protein from Homo sapiens)
- Dna (39-mer) (12 kDa, DNA from synthetic construct)
- Dna replication factor cdt1 (60 kDa, Protein from Homo sapiens)
- H. sapiens occm bound to double stranded dna (1 MDa, Complex)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Dna (39-mer) (12 kDa, DNA from synthetic construct)
- Dna replication licensing factor mcm7 (81 kDa, Protein from Homo sapiens)
- Origin recognition complex subunit 4 (50 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Origin recognition complex subunit 5 (50 kDa, Protein from Homo sapiens)
- Dna replication licensing factor mcm4 (96 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Origin recognition complex subunit 3 (82 kDa, Protein from Homo sapiens)
H. sapiens OC1M bound to double stranded DNA
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 8s0f
Q-score: 0.212
Weissmann F, Greiwe JF, Puhringer T, Eastwood EL, Couves EC, Miller TCR, Diffley JFX, Costa A
Nature (2024) 636 pp. 499-508 [ DOI: doi:10.1038/s41586-024-08263-6 Pubmed: 39604733 ]
- Dna replication licensing factor mcm2 (102 kDa, Protein from Homo sapiens)
- Dna (39-mer) (12 kDa, DNA from synthetic construct)
- Double stranded dna (Complex from synthetic construct)
- Origin recognition complex subunit 2 (66 kDa, Protein from Homo sapiens)
- H. sapiens oc1m bound to double stranded dna (1 MDa, Complex)
- Dna replication licensing factor mcm6 (93 kDa, Protein from Homo sapiens)
- Dna replication licensing factor mcm3 (91 kDa, Protein from Homo sapiens)
- Dna replication licensing factor mcm5 (82 kDa, Protein from Homo sapiens)
- Origin recognition complex subunit 1 (97 kDa, Protein from Homo sapiens)
- Dna replication factor cdt1 (60 kDa, Protein from Homo sapiens)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- H. sapiens oc1m (Complex from Homo sapiens)
- Dna replication licensing factor mcm7 (81 kDa, Protein from Homo sapiens)
- Origin recognition complex subunit 4 (50 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Origin recognition complex subunit 5 (50 kDa, Protein from Homo sapiens)
- Dna replication licensing factor mcm4 (96 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Origin recognition complex subunit 3 (82 kDa, Protein from Homo sapiens)
- Dna (39-mer) (12 kDa, DNA from synthetic construct)