Structure of s. pombe RNA polymerase II in complex with DSIF and Rat1/Rai1
Resolution: 2.67 Å
EM Method: Single-particle
Fitted PDBs: 8qsz
Q-score: 0.539
Kus K, Carrique L, Kecman T, Fournier M, Hassanein SS, Aydin E, Kilchert C, Grimes JM, Vasiljeva L
Nat Commun (2025) 16 pp. 10-10 [ DOI: doi:10.1038/s41467-024-55063-7 Pubmed: 39746995 ]
- Dna-directed rna polymerase ii subunit rpb1 (194 kDa, Protein from Schizosaccharomyces pombe)
- Dna-directed rna polymerase ii subunit rpb3 (33 kDa, Protein from Schizosaccharomyces pombe)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc2 (15 kDa, Protein from Schizosaccharomyces pombe)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase ii subunit rpb2 (138 kDa, Protein from Schizosaccharomyces pombe)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc5 (8 kDa, Protein from Schizosaccharomyces pombe)
- Dna template (40-mer) (14 kDa, DNA from Schizosaccharomyces pombe)
- Dna-directed rna polymerase ii subunit rpb7 (19 kDa, Protein from Schizosaccharomyces pombe)
- Rna (5'-r(p*ap*up*cp*gp*ap*gp*ap*gp*gp*u)-3') (8 kDa, RNA from Schizosaccharomyces pombe)
- Dna-directed rna polymerase ii subunit rpb11 (14 kDa, Protein from Schizosaccharomyces pombe)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc4 (7 kDa, Protein from Schizosaccharomyces pombe)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc3 (14 kDa, Protein from Schizosaccharomyces pombe)
- S. pombe rna polymerase ii in complex with dsif and rat1/rai1 (Complex from Schizosaccharomyces pombe)
- Dna none template (34-mer) (14 kDa, DNA from Schizosaccharomyces pombe)
- Dna-directed rna polymerase ii subunit rpb9,rbp9 (16 kDa, Protein from Schizosaccharomyces pombe)
- Transcription elongation factor spt5 (123 kDa, Protein from Schizosaccharomyces pombe)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc1 (23 kDa, Protein from Schizosaccharomyces pombe)
- 5'-3' exoribonuclease 2 (102 kDa, Protein from Schizosaccharomyces pombe)
Human holo-PIC in the open state
Resolution: 8.6 Å
EM Method: Single-particle
Fitted PDBs: 5iy7
Q-score: 0.105
He Y, Yan C, Fang J, Inouye C, Tjian R, Ivanov I, Nogales E
Nature (2016) 533 pp. 359-365 [ DOI: doi:10.1038/nature17970 Pubmed: 27193682 ]
- Dna-directed rna polymerase ii subunit rpb4 (16 kDa, Protein from Homo sapiens)
- General transcription factor iif subunit 1 (58 kDa, Protein from Homo sapiens)
- General transcription factor iih subunit 4 (52 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb6 (14 kDa, Protein from Homo sapiens)
- Transcription initiation factor iib (34 kDa, Protein from Homo sapiens)
- Rna polymerase ii (520 kDa, Complex from Homo sapiens)
- Scp-y (23 kDa, DNA from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb7 (19 kDa, Protein from Homo sapiens)
- Tfiih basal transcription factor complex helicase xpb subunit (89 kDa, Protein from Homo sapiens)
- Super core promoter (60 kDa, Complex from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb10 (7 kDa, Protein from Homo sapiens)
- Scp-x (23 kDa, DNA from Homo sapiens)
- General transcription factor iif subunit 2 (28 kDa, Protein from Homo sapiens)
- General transcription factor iie subunit 1 (49 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb5 (24 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb11-a (13 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb9 (14 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb2 (134 kDa, Protein from Homo sapiens)
- Transcription elongation factor tfiis (34 kDa, Protein from Homo sapiens)
- Tfiih basal transcription factor complex helicase xpd subunit (87 kDa, Protein from Homo sapiens)
- General transcription factor iih subunit 5 (8 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Transcription initiation factor iia subunit 2 (12 kDa, Protein from Homo sapiens)
- Human holo-pic in the open state (1 MDa, Complex)
- Transcription initiation factor iie subunit beta (33 kDa, Protein from Homo sapiens)
- General transcription factor iih subunit 3 (34 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Transcription initiation factor iia subunit 1 (41 kDa, Protein from Homo sapiens)
- General transcription factor iih subunit 2 (44 kDa, Protein from Homo sapiens)
- General transcription factors (820 kDa, Complex from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb12 (7 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb3 (31 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb1 (217 kDa, Protein from Homo sapiens)
- Tata-box-binding protein (37 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb8 (17 kDa, Protein from Homo sapiens)
Human holo-PIC in the initial transcribing state
Resolution: 7.9 Å
EM Method: Single-particle
Fitted PDBs: 5iy8
Q-score: 0.111
He Y, Yan C, Fang J, Inouye C, Tjian R, Ivanov I, Nogales E
Nature (2016) 533 pp. 359-365 [ DOI: doi:10.1038/nature17970 Pubmed: 27193682 ]
- Dna-directed rna polymerase ii subunit rpb4 (16 kDa, Protein from Homo sapiens)
- General transcription factor iif subunit 1 (58 kDa, Protein from Homo sapiens)
- General transcription factor iih subunit 4 (52 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb6 (14 kDa, Protein from Homo sapiens)
- Transcription initiation factor iib (34 kDa, Protein from Homo sapiens)
- Rna polymerase ii (520 kDa, Complex from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb7 (19 kDa, Protein from Homo sapiens)
- Tfiih basal transcription factor complex helicase xpb subunit (89 kDa, Protein from Homo sapiens)
- Super core promoter (60 kDa, Complex from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb10 (7 kDa, Protein from Homo sapiens)
- Scp-y (25 kDa, DNA from Homo sapiens)
- Transcription elongation factor a protein 1 (34 kDa, Protein from Homo sapiens)
- Human holo-pic in the initial transcribing state (1 MDa, Complex)
- General transcription factor iif subunit 2 (28 kDa, Protein from Homo sapiens)
- General transcription factor iie subunit 1 (49 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb5 (24 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb11-a (13 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb9 (14 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb2 (134 kDa, Protein from Homo sapiens)
- Tfiih basal transcription factor complex helicase xpd subunit (87 kDa, Protein from Homo sapiens)
- General transcription factor iih subunit 5 (8 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Transcription initiation factor iia subunit 2 (12 kDa, Protein from Homo sapiens)
- Transcription initiation factor iie subunit beta (33 kDa, Protein from Homo sapiens)
- General transcription factor iih subunit 3 (34 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Transcription initiation factor iia subunit 1 (41 kDa, Protein from Homo sapiens)
- General transcription factor iih subunit 2 (44 kDa, Protein from Homo sapiens)
- General transcription factors (820 kDa, Complex from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb12 (7 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb3 (31 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb1 (217 kDa, Protein from Homo sapiens)
- Scp-x (25 kDa, DNA from Homo sapiens)
- Tata-box-binding protein (37 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb8 (17 kDa, Protein from Homo sapiens)
Human holo-PIC in the initial transcribing state (no IIS)
Resolution: 6.3 Å
EM Method: Single-particle
Fitted PDBs: 5iy9
Q-score: 0.132
He Y, Yan C, Fang J, Inouye C, Tjian R, Ivanov I, Nogales E
Nature (2016) 533 pp. 359-365 [ DOI: doi:10.1038/nature17970 Pubmed: 27193682 ]
- Dna-directed rna polymerase ii subunit rpb4 (16 kDa, Protein from Homo sapiens)
- General transcription factor iif subunit 1 (58 kDa, Protein from Homo sapiens)
- General transcription factor iih subunit 4 (52 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb6 (14 kDa, Protein from Homo sapiens)
- Transcription initiation factor iib (34 kDa, Protein from Homo sapiens)
- Rna (1 kDa, RNA from Homo sapiens)
- Rna polymerase ii (520 kDa, Complex from Homo sapiens)
- Human holo-pic in the initial transcribing state (no iis) (1 MDa, Complex)
- Dna-directed rna polymerase ii subunit rpb7 (19 kDa, Protein from Homo sapiens)
- Tfiih basal transcription factor complex helicase xpb subunit (89 kDa, Protein from Homo sapiens)
- Super core promoter (60 kDa, Complex from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb10 (7 kDa, Protein from Homo sapiens)
- Scp-y (25 kDa, DNA from Homo sapiens)
- General transcription factor iif subunit 2 (28 kDa, Protein from Homo sapiens)
- General transcription factors (790 kDa, Complex from Homo sapiens)
- General transcription factor iie subunit 1 (49 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb5 (24 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb11-a (13 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb9 (14 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb2 (134 kDa, Protein from Homo sapiens)
- Tfiih basal transcription factor complex helicase xpd subunit (87 kDa, Protein from Homo sapiens)
- General transcription factor iih subunit 5 (8 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Transcription initiation factor iia subunit 2 (12 kDa, Protein from Homo sapiens)
- Transcription initiation factor iie subunit beta (33 kDa, Protein from Homo sapiens)
- General transcription factor iih subunit 3 (34 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Transcription initiation factor iia subunit 1 (41 kDa, Protein from Homo sapiens)
- General transcription factor iih subunit 2 (44 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb12 (7 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb3 (31 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb1 (217 kDa, Protein from Homo sapiens)
- Scp-x (25 kDa, DNA from Homo sapiens)
- Tata-box-binding protein (37 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb8 (17 kDa, Protein from Homo sapiens)
Human core-PIC in the closed state
Resolution: 5.4 Å
EM Method: Single-particle
Fitted PDBs: 5iya
Q-score: 0.169
He Y, Yan C, Fang J, Inouye C, Tjian R, Ivanov I, Nogales E
Nature (2016) 533 pp. 359-365 [ DOI: doi:10.1038/nature17970 Pubmed: 27193682 ]
- Dna-directed rna polymerase ii subunit rpb4 (16 kDa, Protein from Homo sapiens)
- General transcription factor iif subunit 1 (58 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb6 (14 kDa, Protein from Homo sapiens)
- Transcription initiation factor iib (34 kDa, Protein from Homo sapiens)
- Rna polymerase ii (520 kDa, Complex from Homo sapiens)
- General transcription factors (330 kDa, Complex from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb7 (19 kDa, Protein from Homo sapiens)
- Super core promoter (60 kDa, Complex from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb10 (7 kDa, Protein from Homo sapiens)
- Transcription elongation factor a protein 1 (34 kDa, Protein from Homo sapiens)
- General transcription factor iif subunit 2 (28 kDa, Protein from Homo sapiens)
- Scp-x (18 kDa, DNA from Homo sapiens)
- General transcription factor iie subunit 1 (49 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb5 (24 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb11-a (13 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb9 (14 kDa, Protein from Homo sapiens)
- Human holo-pic in the closed state (900 kDa, Complex from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb2 (134 kDa, Protein from Homo sapiens)
- Scp-y (18 kDa, DNA from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Transcription initiation factor iia subunit 2 (12 kDa, Protein from Homo sapiens)
- Transcription initiation factor iie subunit beta (33 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Transcription initiation factor iia subunit 1 (41 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb12 (7 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb3 (31 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb1 (217 kDa, Protein from Homo sapiens)
- Tata-box-binding protein (37 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb8 (17 kDa, Protein from Homo sapiens)
Human core-PIC in the open state
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 5iyb
Q-score: 0.307
He Y, Yan C, Fang J, Inouye C, Tjian R, Ivanov I, Nogales E
Nature (2016) 533 pp. 359-365 [ DOI: doi:10.1038/nature17970 Pubmed: 27193682 ]
- Dna-directed rna polymerase ii subunit rpb4 (16 kDa, Protein from Homo sapiens)
- General transcription factor iif subunit 1 (58 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb6 (14 kDa, Protein from Homo sapiens)
- Transcription initiation factor iib (34 kDa, Protein from Homo sapiens)
- Rna polymerase ii (520 kDa, Complex from Homo sapiens)
- General transcription factors (330 kDa, Complex from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb7 (19 kDa, Protein from Homo sapiens)
- Super core promoter (60 kDa, Complex from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb10 (7 kDa, Protein from Homo sapiens)
- Transcription elongation factor a protein 1 (34 kDa, Protein from Homo sapiens)
- General transcription factor iif subunit 2 (28 kDa, Protein from Homo sapiens)
- General transcription factor iie subunit 1 (49 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb5 (24 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb11-a (13 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb9 (14 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb2 (134 kDa, Protein from Homo sapiens)
- Human core-pic in the open state (900 kDa, Complex from Homo sapiens)
- Scp-y (22 kDa, DNA from Homo sapiens)
- Scp-x (23 kDa, DNA from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Transcription initiation factor iia subunit 2 (12 kDa, Protein from Homo sapiens)
- Transcription initiation factor iie subunit beta (33 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Transcription initiation factor iia subunit 1 (41 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb12 (7 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb3 (31 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb1 (217 kDa, Protein from Homo sapiens)
- Tata-box-binding protein (37 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb8 (17 kDa, Protein from Homo sapiens)
Human core-PIC in the initial transcribing state
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 5iyc
Q-score: 0.306
He Y, Yan C, Fang J, Inouye C, Tjian R, Ivanov I, Nogales E
Nature (2016) 533 pp. 359-365 [ DOI: doi:10.1038/nature17970 Pubmed: 27193682 ]
- Dna-directed rna polymerase ii subunit rpb4 (16 kDa, Protein from Homo sapiens)
- General transcription factor iif subunit 1 (58 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb6 (14 kDa, Protein from Homo sapiens)
- Transcription initiation factor iib (34 kDa, Protein from Homo sapiens)
- Scp-y (24 kDa, DNA from Homo sapiens)
- Rna polymerase ii (520 kDa, Complex from Homo sapiens)
- General transcription factors (330 kDa, Complex from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb7 (19 kDa, Protein from Homo sapiens)
- Super core promoter (60 kDa, Complex from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb10 (7 kDa, Protein from Homo sapiens)
- Transcription elongation factor a protein 1 (34 kDa, Protein from Homo sapiens)
- General transcription factor iif subunit 2 (28 kDa, Protein from Homo sapiens)
- General transcription factor iie subunit 1 (49 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb5 (24 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb11-a (13 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb9 (14 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb2 (134 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Human core-pic in the initial transcribing state (900 kDa, Complex from Homo sapiens)
- Transcription initiation factor iia subunit 2 (12 kDa, Protein from Homo sapiens)
- Transcription initiation factor iie subunit beta (33 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Transcription initiation factor iia subunit 1 (41 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb12 (7 kDa, Protein from Homo sapiens)
- Scp-x (24 kDa, DNA from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb3 (31 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb1 (217 kDa, Protein from Homo sapiens)
- Tata-box-binding protein (37 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb8 (17 kDa, Protein from Homo sapiens)
Human core-PIC in the initial transcribing state (no IIS)
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 5iyd
Q-score: 0.322
He Y, Yan C, Fang J, Inouye C, Tjian R, Ivanov I, Nogales E
Nature (2016) 533 pp. 359-365 [ DOI: doi:10.1038/nature17970 Pubmed: 27193682 ]
- Dna-directed rna polymerase ii subunit rpb4 (16 kDa, Protein from Homo sapiens)
- General transcription factor iif subunit 1 (58 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb6 (14 kDa, Protein from Homo sapiens)
- Transcription initiation factor iib (34 kDa, Protein from Homo sapiens)
- Scp-y (24 kDa, DNA from Homo sapiens)
- Rna (1 kDa, RNA from Homo sapiens)
- Rna polymerase ii (520 kDa, Complex from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb7 (19 kDa, Protein from Homo sapiens)
- Super core promoter (60 kDa, Complex from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb10 (7 kDa, Protein from Homo sapiens)
- General transcription factor iif subunit 2 (28 kDa, Protein from Homo sapiens)
- General transcription factor iie subunit 1 (49 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb5 (24 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb11-a (13 kDa, Protein from Homo sapiens)
- General transcription factors (300 kDa, Complex from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb2 (134 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb9 (14 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Transcription initiation factor iia subunit 2 (12 kDa, Protein from Homo sapiens)
- Transcription initiation factor iie subunit beta (33 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Transcription initiation factor iia subunit 1 (41 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb12 (7 kDa, Protein from Homo sapiens)
- Scp-x (24 kDa, DNA from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb3 (31 kDa, Protein from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb1 (217 kDa, Protein from Homo sapiens)
- Tata-box-binding protein (37 kDa, Protein from Homo sapiens)
- Human core-pic in the initial transcribing state (no iis) (870 kDa, Complex from Homo sapiens)
- Dna-directed rna polymerase ii subunit rpb8 (17 kDa, Protein from Homo sapiens)
Architecture of the RNA polymerase II-Mediator core transcription initiation complex
Resolution: 6.6 Å
EM Method: Single-particle
Fitted PDBs: 4v1m
Q-score: 0.151
Plaschka C, Lariviere L, Wenzeck L, Hemann M, Tegunov D, Petrotchenko EV, Borchers CH, Baumeister W, Herzog F, Villa E, Cramer P
Nature (2015) 518 pp. 376-380 [ DOI: doi:10.1038/nature14229 Pubmed: 25652824 ]
- Template dna (DNA from synthetic construct)
- Dna-directed rna polymerase ii subunit rpb11 (13 kDa, Protein from Saccharomyces cerevisiae)
- Dna-directed rna polymerase ii subunit rpb3 (35 kDa, Protein from Saccharomyces cerevisiae)
- Nontemplate dna (DNA from synthetic construct)
- Rna (RNA from synthetic construct)
- Pol ii-dna/rna (570 kDa, Sample)
- Dna-directed rna polymerase ii subunit rpb7 (19 kDa, Protein from Saccharomyces cerevisiae)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc 5 (8 kDa, Protein from Saccharomyces cerevisiae)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc 3 (17 kDa, Protein from Saccharomyces cerevisiae)
- Dna-directed rna polymerase ii subunit rpb9 (14 kDa, Protein from Saccharomyces cerevisiae)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc 1 (25 kDa, Protein from Saccharomyces cerevisiae)
- Dna-directed rna polymerase ii subunit rpb1 (192 kDa, Protein from Saccharomyces cerevisiae)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc 2 (18 kDa, Protein from Saccharomyces cerevisiae)
- Dna-directed rna polymerase ii subunit rpb4 (25 kDa, Protein from Saccharomyces cerevisiae)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc 4 (8 kDa, Protein from Saccharomyces cerevisiae)
- Dna-directed rna polymerase ii subunit rpb2 (139 kDa, Protein from Saccharomyces cerevisiae)
Architecture of the RNA polymerase II-Mediator core transcription initiation complex
Resolution: 7.8 Å
EM Method: Single-particle
Fitted PDBs: 4v1n
Q-score: 0.129
Plaschka C, Lariviere L, Wenzeck L, Hemann M, Tegunov D, Petrotchenko EV, Borchers CH, Baumeister W, Herzog F, Villa E, Cramer P
Nature (2015) 518 pp. 376-380 [ DOI: doi:10.1038/nature14229 Pubmed: 25652824 ]
- Transcription initiation factor iif subunit alpha (82 kDa, Protein from Saccharomyces mikatae)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc 1 (25 kDa, Protein from Saccharomyces cerevisiae)
- Transcription initiation factor iib (38 kDa, Protein from Saccharomyces cerevisiae)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc 3 (17 kDa, Protein from Saccharomyces cerevisiae)
- Dna-directed rna polymerase ii subunit rpb4 (25 kDa, Protein from Saccharomyces cerevisiae)
- Dna-directed rna polymerase ii subunit rpb2 (139 kDa, Protein from Saccharomyces cerevisiae)
- Template dna (DNA from synthetic construct)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc 5 (8 kDa, Protein from Saccharomyces cerevisiae)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc 4 (8 kDa, Protein from Saccharomyces cerevisiae)
- Tata-box-binding protein (20 kDa, Protein from Saccharomyces cerevisiae)
- Rna (RNA from synthetic construct)
- Dna-directed rna polymerases i, ii, and iii subunit rpabc 2 (18 kDa, Protein from Saccharomyces cerevisiae)
- Nontemplate dna (DNA from synthetic construct)
- Citc (750 kDa, Sample)
- Dna-directed rna polymerase ii subunit rpb7 (19 kDa, Protein from Saccharomyces cerevisiae)
- Transcription initiation factor iif subunit beta (47 kDa, Protein from Saccharomyces cerevisiae)
- Dna-directed rna polymerase ii subunit rpb9 (14 kDa, Protein from Saccharomyces cerevisiae)
- Dna-directed rna polymerase ii subunit rpb3 (35 kDa, Protein from Saccharomyces cerevisiae)
- Dna-directed rna polymerase ii subunit rpb1 (192 kDa, Protein from Saccharomyces cerevisiae)
- Dna-directed rna polymerase ii subunit rpb11 (13 kDa, Protein from Saccharomyces cerevisiae)