Middle assembly intermediate of the Trypanosoma brucei mitoribosomal small subunit
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 7pua
Q-score: 0.436
Lenarcic T, Niemann M, Ramrath DJF, Calderaro S, Flugel T, Saurer M, Leibundgut M, Boehringer D, Prange C, Horn EK, Schneider A, Ban N
PNAS (2022) 119 [ DOI: doi:10.1073/pnas.2114710118 Pubmed: 35042777 ]
- Ms29 (57 kDa, Protein from Trypanosoma brucei brucei)
- Ms54 (72 kDa, Protein from Trypanosoma brucei brucei)
- Unkc (783 Da, Protein from Trypanosoma brucei brucei)
- Ms74 (8 kDa, Protein from Trypanosoma brucei brucei)
- Water (18 Da, Ligand)
- Ms50 (131 kDa, Protein from Trypanosoma brucei brucei)
- Ppr_long domain-containing protein (116 kDa, Protein from Trypanosoma brucei brucei)
- Us14m (19 kDa, Protein from Trypanosoma brucei brucei)
- Unk8 (2 kDa, Protein from Trypanosoma brucei brucei)
- Unkf (3 kDa, Protein from Trypanosoma brucei brucei)
- Lmwpc domain-containing protein (30 kDa, Protein from Trypanosoma brucei brucei)
- Ms23 (36 kDa, Protein from Trypanosoma brucei brucei)
- Ubiquitin-like domain-containing protein (26 kDa, Protein from Trypanosoma brucei brucei)
- Acyl transferase-like protein, putative (60 kDa, Protein from Trypanosoma brucei brucei)
- Acyl carrier protein (16 kDa, Protein from Trypanosoma brucei brucei)
- Ms53 (75 kDa, Protein from Trypanosoma brucei brucei)
- Translation initiation factor if-2, putative (87 kDa, Protein from Trypanosoma brucei brucei)
- Ms35 (98 kDa, Protein from Trypanosoma brucei brucei)
- 9s rrna (198 kDa, RNA from Trypanosoma brucei brucei)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Bs16m (22 kDa, Protein from Trypanosoma brucei brucei)
- Ms59 (35 kDa, Protein from Trypanosoma brucei brucei)
- Unkp (2 kDa, Protein from Trypanosoma brucei brucei)
- Zinc ion (65 Da, Ligand)
- Mt-saf21 (39 kDa, Protein from Trypanosoma brucei brucei)
- Mt-saf37 (37 kDa, Protein from Trypanosoma brucei brucei)
- 9s rrna (919 Da, RNA from Trypanosoma brucei brucei)
- Us15m (50 kDa, Protein from Trypanosoma brucei brucei)
- Unkk (2 kDa, Protein from Trypanosoma brucei brucei)
- Magnesium ion (24 Da, Ligand)
- Unk (698 Da, Protein from Trypanosoma brucei brucei)
- Mt-saf23 (38 kDa, Protein from Trypanosoma brucei brucei)
- Ms37 (24 kDa, Protein from Trypanosoma brucei brucei)
- Ms72 (19 kDa, Protein from Trypanosoma brucei brucei)
- Ms70 (21 kDa, Protein from Trypanosoma brucei brucei)
- Mt-saf3 (106 kDa, Protein from Trypanosoma brucei brucei)
- Ms65 (30 kDa, Protein from Trypanosoma brucei brucei)
- Unka (869 Da, Protein from Trypanosoma brucei brucei)
- Ms26 (51 kDa, Protein from Trypanosoma brucei brucei)
- 30s ribosomal protein s17, putative (35 kDa, Protein from Trypanosoma brucei brucei)
- Ms58 (35 kDa, Protein from Trypanosoma brucei brucei)
- Protein fyv4, mitochondrial (22 kDa, Protein from Trypanosoma brucei brucei)
- Bs21m (23 kDa, Protein from Trypanosoma brucei brucei)
- Ms62 (32 kDa, Protein from Trypanosoma brucei brucei)
- Unki (528 Da, Protein from Trypanosoma brucei brucei)
- Ms34 (28 kDa, Protein from Trypanosoma brucei brucei)
- 30s ribosomal protein s8, putative (32 kDa, Protein from Trypanosoma brucei brucei)
- Ms38 (28 kDa, Protein from Trypanosoma brucei brucei)
- Ms71 (20 kDa, Protein from Trypanosoma brucei brucei)
- Us3m (9 kDa, Protein from Trypanosoma brucei brucei)
- Mt-saf9 (69 kDa, Protein from Trypanosoma brucei brucei)
- Mt-saf39 (89 kDa, Protein from Trypanosoma brucei brucei)
- Unkd (1 kDa, Protein from Trypanosoma brucei brucei)
- Duf4379 domain-containing protein (16 kDa, Protein from Trypanosoma brucei brucei)
- Mt-saf7 (76 kDa, Protein from Trypanosoma brucei brucei)
- Ms56 (47 kDa, Protein from Trypanosoma brucei brucei)
- Bs6m (18 kDa, Protein from Trypanosoma brucei brucei)
- Us12m (10 kDa, Protein from Trypanosoma brucei brucei)
- Dna photolyase, putative (95 kDa, Protein from Trypanosoma brucei brucei)
- Ms22 (73 kDa, Protein from Trypanosoma brucei brucei)
- Bs18m (36 kDa, Protein from Trypanosoma brucei brucei)
- Ms73 (11 kDa, Protein from Trypanosoma brucei brucei)
- Mt-saf29 (20 kDa, Protein from Trypanosoma brucei brucei)
- Mt-saf38 (69 kDa, Protein from Trypanosoma brucei brucei)
- Ms57 (45 kDa, Protein from Trypanosoma brucei brucei)
- Phosphate ion (94 Da, Ligand)
- Mt-saf5 (86 kDa, Protein from Trypanosoma brucei brucei)
- Mt-saf27 (26 kDa, Protein from Trypanosoma brucei brucei)
- Us11m (37 kDa, Protein from Trypanosoma brucei brucei)
- Unkg (1 kDa, Protein from Trypanosoma brucei brucei)
- Us19m (28 kDa, Protein from Trypanosoma brucei brucei)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Lysm domain-containing protein (94 kDa, Protein from Trypanosoma brucei brucei)
- Unkg (23 kDa, Protein from Trypanosoma brucei brucei)
- Ms55 (66 kDa, Protein from Trypanosoma brucei brucei)
- Ms51 (93 kDa, Protein from Trypanosoma brucei brucei)
- Ms49 (137 kDa, Protein from Trypanosoma brucei brucei)
- Ms52 (84 kDa, Protein from Trypanosoma brucei brucei)
- Dihydroflavine-adenine dinucleotide (787 Da, Ligand)
- S-(2-{[n-(2-hydroxy-4-{[hydroxy(oxido)phosphino]oxy}-3,3-dimethylbutanoyl)-beta-alanyl]amino}ethyl) decanethioate (496 Da, Ligand)
- Rhodanese domain-containing protein (30 kDa, Protein from Trypanosoma brucei brucei)
- Ms63 (31 kDa, Protein from Trypanosoma brucei brucei)
- Mt-saf22 (39 kDa, Protein from Trypanosoma brucei brucei)
- Duf4460 domain-containing protein (76 kDa, Protein from Trypanosoma brucei brucei)
- Ribosomal_s5_c domain-containing protein (50 kDa, Protein from Trypanosoma brucei brucei)
- Ms69 (21 kDa, Protein from Trypanosoma brucei brucei)
- Mt-saf31 (17 kDa, Protein from Trypanosoma brucei brucei)
- Middle assembly intermediate of the trypanosoma brucei mitoribosomal small subunit (Complex from Trypanosoma brucei brucei)
- Us9m (50 kDa, Protein from Trypanosoma brucei brucei)
- Ms33 (21 kDa, Protein from Trypanosoma brucei brucei)
State A of the Trypanosoma brucei mitoribosomal large subunit assembly intermediate
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 6yxx
Q-score: 0.389
Jaskolowski M, Ramrath DJF, Bieri P, Niemann M, Mattei S, Calderaro S, Leibundgut M, Horn EK, Boehringer D, Schneider A, Ban N
Mol Cell (2020) 79 pp. 629-644.e4 [ DOI: doi:10.1016/j.molcel.2020.06.030 Pubmed: 32679035 ]
- Spou_methylase domain-containing protein (40 kDa, Protein from Trypanosoma brucei brucei)
- L51_s25_ci-b8 domain-containing protein (31 kDa, Protein from Trypanosoma brucei brucei)
- Ml76 (39 kDa, Protein from Trypanosoma brucei brucei)
- Bl35m (21 kDa, Protein from Trypanosoma brucei brucei)
- Ribosomal protein l4/l1 family, putative (53 kDa, Protein from Trypanosoma brucei brucei)
- Mt-laf14 (82 kDa, Protein from Trypanosoma brucei brucei)
- Mt-laf7 (18 kDa, Protein from Trypanosoma brucei brucei)
- Ml63 (18 kDa, Protein from Trypanosoma brucei brucei)
- Mt-laf19 (12 kDa, Protein from Trypanosoma brucei brucei)
- Mt-laf25 (32 kDa, Protein from Trypanosoma brucei brucei)
- Ml80 (34 kDa, Protein from Trypanosoma brucei brucei)
- Ml78 (35 kDa, Protein from Trypanosoma brucei brucei)
- Ribosomal_l9 domain-containing protein (30 kDa, Protein from Trypanosoma brucei brucei)
- Chaperone protein dnaj, putative (43 kDa, Protein from Trypanosoma brucei brucei)
- Ml71 (50 kDa, Protein from Trypanosoma brucei brucei)
- Kripp9 (89 kDa, Protein from Trypanosoma brucei brucei)
- Ml84 (24 kDa, Protein from Trypanosoma brucei brucei)
- Ribosomal_l18e/l15p domain-containing protein (43 kDa, Protein from Trypanosoma brucei brucei)
- Ml74 (39 kDa, Protein from Trypanosoma brucei brucei)
- Ml98 (12 kDa, Protein from Trypanosoma brucei brucei)
- Unk (1 kDa, Protein from Trypanosoma brucei brucei)
- Ml93 (18 kDa, Protein from Trypanosoma brucei brucei)
- Mt-laf12 (77 kDa, Protein from Trypanosoma brucei brucei)
- Acyl carrier protein (16 kDa, Protein from Trypanosoma brucei brucei)
- Zinc ion (65 Da, Ligand)
- Mt-laf29 (38 kDa, Protein from Trypanosoma brucei brucei)
- Peptidyl-prolyl cis-trans isomerase (25 kDa, Protein from Trypanosoma brucei brucei)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Mt-laf15a (34 kDa, Protein from Trypanosoma brucei brucei)
- Ul24m (43 kDa, Protein from Trypanosoma brucei brucei)
- Unk (7 kDa, Protein from Trypanosoma brucei brucei)
- Ml41 (22 kDa, Protein from Trypanosoma brucei brucei)
- Ml85 (22 kDa, Protein from Trypanosoma brucei brucei)
- Ml49 (24 kDa, Protein from Trypanosoma brucei brucei)
- Pseudouridylate synthase, putative (46 kDa, Protein from Trypanosoma brucei brucei)
- Mt-laf4 (69 kDa, Protein from Trypanosoma brucei brucei)
- State b of the trypanosoma brucei mitoribosomal large subunit assembly intermediate (Complex from Trypanosoma brucei brucei)
- Bl21m (20 kDa, Protein from Trypanosoma brucei brucei)
- Dead-box helicase, putative (85 kDa, Protein from Trypanosoma brucei brucei)
- 50s ribosomal protein l13, putative (24 kDa, Protein from Trypanosoma brucei brucei)
- Unk (869 Da, Protein from Trypanosoma brucei brucei)
- Sodium ion (22 Da, Ligand)
- Mt-laf24 (49 kDa, Protein from Trypanosoma brucei brucei)
- S-(2-{[n-(2-hydroxy-4-{[hydroxy(oxido)phosphino]oxy}-3,3-dimethylbutanoyl)-beta-alanyl]amino}ethyl) decanethioate (496 Da, Ligand)
- Ml53 (35 kDa, Protein from Trypanosoma brucei brucei)
- Unk (698 Da, Protein from Trypanosoma brucei brucei)
- Water (18 Da, Ligand)
- Bl20m (24 kDa, Protein from Trypanosoma brucei brucei)
- Tetratricopeptide repeat (49 kDa, Protein from Trypanosoma brucei brucei)
- Ml94 (17 kDa, Protein from Trypanosoma brucei brucei)
- Ml67 (93 kDa, Protein from Trypanosoma brucei brucei)
- Nicotinamide-adenine-dinucleotide (663 Da, Ligand)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Ml86 (22 kDa, Protein from Trypanosoma brucei brucei)
- Ul22m (32 kDa, Protein from Trypanosoma brucei brucei)
- Bl19m (16 kDa, Protein from Trypanosoma brucei brucei)
- Ml88 (22 kDa, Protein from Trypanosoma brucei brucei)
- Unk (2 kDa, Protein from Trypanosoma brucei brucei)
- Mt-laf8 (68 kDa, Protein from Trypanosoma brucei brucei)
- Peptidyl-prolyl cis-trans isomerase (20 kDa, Protein from Trypanosoma brucei brucei)
- Gtp-binding protein, putative (50 kDa, Protein from Trypanosoma brucei brucei)
- Ml95 (17 kDa, Protein from Trypanosoma brucei brucei)
- Duf4379 domain-containing protein (63 kDa, Protein from Trypanosoma brucei brucei)
- Ml89 (22 kDa, Protein from Trypanosoma brucei brucei)
- Mt-laf27 (11 kDa, Protein from Trypanosoma brucei brucei)
- Ml87 (22 kDa, Protein from Trypanosoma brucei brucei)
- Mt-enga (65 kDa, Protein from Trypanosoma brucei brucei)
- Ml81 (29 kDa, Protein from Trypanosoma brucei brucei)
- Magnesium ion (24 Da, Ligand)
- Ml64 (28 kDa, Protein from Trypanosoma brucei brucei)
- Ml68 (62 kDa, Protein from Trypanosoma brucei brucei)
- 50s ribosomal protein l17, putative (35 kDa, Protein from Trypanosoma brucei brucei)
- Unk (2 kDa, Protein from Trypanosoma brucei brucei)
- Ul30m (25 kDa, Protein from Trypanosoma brucei brucei)
- Ribosomal protein l3 mitochondrial, putative (55 kDa, Protein from Trypanosoma brucei brucei)
- Ml42 (21 kDa, Protein from Trypanosoma brucei brucei)
- Mt-laf21 (56 kDa, Protein from Trypanosoma brucei brucei)
- Ul29m (54 kDa, Protein from Trypanosoma brucei brucei)
- Unk (6 kDa, Protein from Trypanosoma brucei brucei)
- Bl28m (28 kDa, Protein from Trypanosoma brucei brucei)
- Ribosomal protein l11, putative (40 kDa, Protein from Trypanosoma brucei brucei)
- Mt-laf23 (63 kDa, Protein from Trypanosoma brucei brucei)
- Ml70 (62 kDa, Protein from Trypanosoma brucei brucei)
- Ml75 (39 kDa, Protein from Trypanosoma brucei brucei)
- 12s ribosomal rna (365 kDa, RNA from Trypanosoma brucei brucei)
- Bl32m (9 kDa, Protein from Trypanosoma brucei brucei)
- Ul23m (28 kDa, Protein from Trypanosoma brucei brucei)
- Spou_methylase domain-containing protein (67 kDa, Protein from Trypanosoma brucei brucei)
- Unk (2 kDa, Protein from Trypanosoma brucei brucei)
- Unk (20 kDa, Protein from Trypanosoma brucei brucei)
- Ml52 (167 kDa, Protein from Trypanosoma brucei brucei)
- Kripp3 (58 kDa, Protein from Trypanosoma brucei brucei)
- Lipase (class 3) (58 kDa, Protein from Trypanosoma brucei brucei)