Outer kinetochore Dam1 protomer dimer Ndc80-Nuf2 coiled-coil complex
Resolution: 3.15 Å
EM Method: Single-particle
Fitted PDBs: 8q84
Q-score: 0.294
Muir KW, Batters C, Dendooven T, Yang J, Zhang Z, Burt A, Barford D
Science (2023) 382 pp. 1184-1190 [ Pubmed: 38060647 DOI: doi:10.1126/science.adj8736 ]
- Dash complex subunit dam1 (38 kDa, Protein from Saccharomyces cerevisiae)
- Dash complex subunit duo1 (27 kDa, Protein from Saccharomyces cerevisiae)
- Outer kinetochore dam1 protomer dimer with staple and ndc80-nuf2 coiled-coils (673 GDa, Complex from Saccharomyces cerevisiae)
- Kinetochore protein nuf2 (53 kDa, Protein from Saccharomyces cerevisiae)
- Dash complex subunit spc19 (18 kDa, Protein from Saccharomyces cerevisiae)
- Kinetochore protein ndc80 (80 kDa, Protein from Saccharomyces cerevisiae)
- Dash complex subunit spc34 (34 kDa, Protein from Saccharomyces cerevisiae)
- Dash complex subunit hsk3 (8 kDa, Protein from Saccharomyces cerevisiae)
- Dash complex subunit dad3 (10 kDa, Protein from Saccharomyces cerevisiae)
- Dash complex subunit dad2 (15 kDa, Protein from Saccharomyces cerevisiae)
- Dash complex subunit dad4 (8 kDa, Protein from Saccharomyces cerevisiae)
- Dash complex subunit ask1 (32 kDa, Protein from Saccharomyces cerevisiae)
- Dash complex subunit dad1 (10 kDa, Protein from Saccharomyces cerevisiae)
Outer kinetochore Dam1 protomer monomer Ndc80-Nuf2 coiled-coil complex
Resolution: 3.97 Å
EM Method: Single-particle
Fitted PDBs: 8q85
Q-score: 0.277
Muir KW, Batters C, Dendooven T, Yang J, Zhang Z, Burt A, Barford D
Science (2023) 382 pp. 1184-1190 [ Pubmed: 38060647 DOI: doi:10.1126/science.adj8736 ]
- Dash complex subunit dam1 (38 kDa, Protein from Saccharomyces cerevisiae)
- Dash complex subunit duo1 (27 kDa, Protein from Saccharomyces cerevisiae)
- Kinetochore protein nuf2 (53 kDa, Protein from Saccharomyces cerevisiae)
- Dash complex subunit spc19 (18 kDa, Protein from Saccharomyces cerevisiae)
- Kinetochore protein ndc80 (80 kDa, Protein from Saccharomyces cerevisiae)
- Dash complex subunit spc34 (34 kDa, Protein from Saccharomyces cerevisiae)
- Dash complex subunit hsk3 (8 kDa, Protein from Saccharomyces cerevisiae)
- Dash complex subunit dad3 (10 kDa, Protein from Saccharomyces cerevisiae)
- Dash complex subunit dad2 (15 kDa, Protein from Saccharomyces cerevisiae)
- Dash complex subunit dad4 (8 kDa, Protein from Saccharomyces cerevisiae)
- Dash complex subunit ask1 (32 kDa, Protein from Saccharomyces cerevisiae)
- Outer kinetochore dam1 protomer monomer with staple and ndc80-nuf2 coiled-coils (673 GDa, Complex from Saccharomyces cerevisiae)
- Dash complex subunit dad1 (10 kDa, Protein from Saccharomyces cerevisiae)
CryoEM Structure INO80core Hexasome complex Rvb core refinement state1
Resolution: 2.93 Å
EM Method: Single-particle
Fitted PDBs: 8ooc
Q-score: 0.568
Zhang M, Jungblut A, Kunert F, Hauptmann L, Hoffmann T, Kolesnikova O, Metzner F, Moldt M, Weis F, DiMaio F, Hopfner KP, Eustermann S
Science (2023) 381 pp. 313-319 [ Pubmed: 37384673 DOI: doi:10.1126/science.adf6287 ]
- Chromatin-remodeling atpase ino80 (130 kDa, Protein from Thermochaetoides thermophila)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Ruvb-like helicase (53 kDa, Protein from Thermochaetoides thermophila)
- Dash complex subunit dad4 (87 kDa, Protein from Thermochaetoides thermophila)
- Ino80 core module in complex with hexasome (861 kDa, Complex from Thermochaetoides thermophila)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Ino80 complex subunit b-like conserved region domain-containing protein (53 kDa, Protein from Thermochaetoides thermophila)
- Ruvb-like helicase (50 kDa, Protein from Thermochaetoides thermophila)
- Vps72/yl1 c-terminal domain-containing protein (23 kDa, Protein from Thermochaetoides thermophila)
Negative stain EM 3D Reconstruction of the Dam1cBim1p complex.
Dudziak A, Engelhard L, Bourque C, Klink BU, Rombaut P, Kornakov N, Janen K, Herzog F, Gatsogiannis C, Westermann S
EMBO J (2021) 40 pp. e108004-e108004 [ Pubmed: 34313341 DOI: doi:10.15252/embj.2021108004 ]
- Dam1pbim1p complex (Complex from Saccharomyces cerevisiae)
- Spc19 protein of the dam1c from s. cerevisiae (Protein from Saccharomyces cerevisiae)
- Dad1p of the dam1c from s. cerevisiae (Protein from Saccharomyces cerevisiae)
- Dad2p of the dam1c from s. cerevisiae (Protein from Saccharomyces cerevisiae)
- Dad4p of the dam1c from s. cerevisiae (Protein from Saccharomyces cerevisiae)
- Dad3p of the dam1c from s. cerevisiae (Protein from Saccharomyces cerevisiae)
- Bim1p of s. cerevisiae (Protein from Saccharomyces cerevisiae)
- Dam1 protein of the dam1c from s. cerevisiae (Protein from Saccharomyces cerevisiae)
- Hsk3p of the dam1c from s. cerevisiae (Protein from Saccharomyces cerevisiae)
- Duo1 protein of the dam1c from s. cerevisiae (Protein from Saccharomyces cerevisiae)
- Ask1 protein of the dam1c from s. cerevisiae (Protein from Saccharomyces cerevisiae)
Negative stain EM 3D reconstruction of the Dam1 / DASH complex.
Dudziak A, Engelhard L, Bourque C, Klink BU, Rombaut P, Kornakov N, Janen K, Herzog F, Gatsogiannis C, Westermann S
EMBO J (2021) 40 pp. e108004-e108004 [ Pubmed: 34313341 DOI: doi:10.15252/embj.2021108004 ]
- A dimer of the dam1 / dash complex. (3 MDa, Complex from Saccharomyces cerevisiae)
- Hsk3p of the dam1c of s. cerevisiae (Protein from Saccharomyces cerevisiae)
- Spc34p of the dam1c of s. cerevisiae (Protein from Saccharomyces cerevisiae)
- Duo1p of the dam1c of s. cerevisiae (Protein from Saccharomyces cerevisiae)
- Dad1p of the dam1c of s. cerevisiae (Protein from Saccharomyces cerevisiae)
- Spc19p of the dam1c of s. cerevisiae (Protein from Saccharomyces cerevisiae)
- Dad2p of the dam1c of s. cerevisiae (Protein from Saccharomyces cerevisiae)
- Dad3p of the dam1c of s. cerevisiae (Protein from Saccharomyces cerevisiae)
- Dad4p of the dam1c of s. cerevisiae (Protein from Saccharomyces cerevisiae)
- Ask1p of the dam1c of s. cerevisiae (Protein from Saccharomyces cerevisiae)
- Dam1p of the dam1c of s. cerevisiae (Protein from Saccharomyces cerevisiae)
Science (2018) 360 pp. 552-558 [ DOI: doi:10.1126/science.aar6436 Pubmed: 29724956 ]
- Dad1 (Protein from Chaetomium thermophilum)
- Dad2 (Protein from Chaetomium thermophilum)
- Duo1 (Protein from Chaetomium thermophilum)
- Dad3 (Protein from Chaetomium thermophilum)
- Dad4 (Protein from Chaetomium thermophilum)
- Hsk3 (Protein from Chaetomium thermophilum)
- Dam1 (Protein from Chaetomium thermophilum)
- Dash/dam1 complex (115 kDa, Complex from Chaetomium thermophilum)
- Spc19 (Protein from Chaetomium thermophilum)
- Ask1 (Protein from Chaetomium thermophilum)
- Spc34 (Protein from Chaetomium thermophilum)
Resolution: 4.5 Å
EM Method: Single-particle
Fitted PDBs: 6cfz
Q-score: 0.208
Science (2018) 360 pp. 552-558 [ DOI: doi:10.1126/science.aar6436 Pubmed: 29724956 ]
- Spc34 (27 kDa, Protein from Chaetomium thermophilum)
- Ask1 (8 kDa, Protein from Chaetomium thermophilum)
- Spc19 (17 kDa, Protein from Chaetomium thermophilum)
- Dad1,dad1 (10 kDa, Protein from Chaetomium thermophilum)
- Dad4 (8 kDa, Protein from Chaetomium thermophilum)
- Dash/dam1 complex (115 kDa, Complex from Chaetomium thermophilum)
- Dad2 (10 kDa, Protein from Chaetomium thermophilum)
- Hsk3 (6 kDa, Protein from Chaetomium thermophilum)
- Dam1 (6 kDa, Protein from Chaetomium thermophilum)
- Duo1 (12 kDa, Protein from Chaetomium thermophilum)
- Dad3 (7 kDa, Protein from Chaetomium thermophilum)