Structure of outer-arm dyneins bound to microtubule with microtubule binding state 1(MTBS-1)
Resolution: 4.0 Å
EM Method: Single-particle
Fitted PDBs: 7k58
Q-score: 0.357
Rao Q, Han L, Wang Y, Chai P, Kuo YW, Yang R, Hu F, Yang Y, Howard J, Zhang K
Nat Struct Mol Biol (2021) 28 pp. 799-810 [ Pubmed: 34556869 DOI: doi:10.1038/s41594-021-00656-9 ]
- Dynein light chain roadblock (10 kDa, Protein from Tetrahymena thermophila)
- Outer-arm dynein (2 MDa, Complex from Tetrahymena thermophila)
- Dynein light chain tctex-type 1 protein (12 kDa, Protein from Tetrahymena thermophila)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Dynein light chain (11 kDa, Protein from Tetrahymena thermophila)
- Dynein light chain (10 kDa, Protein from Tetrahymena thermophila)
- Dynein light chain roadblock-type 2 protein (12 kDa, Protein from Tetrahymena thermophila)
- Dynein light chain (11 kDa, Protein from Tetrahymena thermophila)
- Dynein light chain (11 kDa, Protein from Tetrahymena thermophila)
- Magnesium ion (24 Da, Ligand)
- Gamma heavy chain (450 kDa, Protein from Tetrahymena thermophila)
- Thioredoxin (12 kDa, Protein from Tetrahymena thermophila)
- Dynein light chain (10 kDa, Protein from Tetrahymena thermophila)
- Dynein heavy chain, outer arm protein (533 kDa, Protein from Tetrahymena thermophila)
- Dynein intermediate chain 2 (69 kDa, Protein from Tetrahymena thermophila)
- Dynein light chain 1 (21 kDa, Protein from Tetrahymena thermophila)
- Dynein light chain (10 kDa, Protein from Tetrahymena thermophila)
- Dynein light chain 2a (14 kDa, Protein from Tetrahymena thermophila)
- Flagellar outer dynein arm intermediate protein, putative (63 kDa, Protein from Tetrahymena thermophila)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Outer arm dynein beta heavy chain (529 kDa, Protein from Tetrahymena thermophila)
Structure of outer-arm dynein bound to microtubule doublet in microtubule binding state 2 (MTBS-2)
Resolution: 4.5 Å
EM Method: Single-particle
Fitted PDBs: 7k5b
Q-score: 0.264
Rao Q, Han L, Wang Y, Chai P, Kuo YW, Yang R, Hu F, Yang Y, Howard J, Zhang K
Nat Struct Mol Biol (2021) 28 pp. 799-810 [ Pubmed: 34556869 DOI: doi:10.1038/s41594-021-00656-9 ]
- Dynein light chain (11 kDa, Protein from Tetrahymena thermophila)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Dynein light chain (11 kDa, Protein from Tetrahymena thermophila)
- Dynein light chain (12 kDa, Protein from Tetrahymena thermophila)
- Dynein light chain (10 kDa, Protein from Tetrahymena thermophila)
- Dynein heavy chain, outer arm protein (533 kDa, Protein from Tetrahymena thermophila)
- Dynein light chain 4a (16 kDa, Protein from Tetrahymena thermophila)
- Dynein light chain 1 (21 kDa, Protein from Tetrahymena thermophila)
- Dynein light chain roadblock-type 2 protein (17 kDa, Protein from Tetrahymena thermophila)
- Dynein light chain roadblock-type 2 protein (14 kDa, Protein from Tetrahymena thermophila)
- Magnesium ion (24 Da, Ligand)
- Thioredoxin (12 kDa, Protein from Tetrahymena thermophila)
- Outer arm dynein beta heavy chain (529 kDa, Protein from Tetrahymena thermophila)
- Dynein light chain (10 kDa, Protein from Tetrahymena thermophila)
- Dynein light chain tctex-type 1 protein (12 kDa, Protein from Tetrahymena thermophila)
- Dynein intermediate chain 2 (69 kDa, Protein from Tetrahymena thermophila)
- Outer-arm dynein bound to microtubule doublet in microtubule binding state 2 (mtbs-2) (Complex from Tetrahymena thermophila)
- Dynein light chain (10 kDa, Protein from Tetrahymena thermophila)
- Dynein light chain 2a (14 kDa, Protein from Tetrahymena thermophila)
- Flagellar outer dynein arm intermediate protein, putative (63 kDa, Protein from Tetrahymena thermophila)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Gamma heavy chain (450 kDa, Protein from Tetrahymena thermophila)
The structure of the Tetrahymena thermophila outer dynein arm on doublet microtubule
Resolution: 8.0 Å
EM Method: Single-particle
Fitted PDBs: 7moq
Q-score: 0.067
Kubo S, Yang SK, Black CS, Dai D, Valente-Paterno M, Gaertig J, Ichikawa M, Bui KH
EMBO Rep (2021) 22 pp. e52911-e52911 [ DOI: doi:10.15252/embr.202152911 Pubmed: 34338432 ]
- Docking complex 1 protein (64 kDa, Protein from Tetrahymena thermophila CU428)
- Dynein-1-alpha heavy chain, flagellar inner arm i1 complex protein, putative (475 kDa, Protein from Tetrahymena thermophila CU428)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Dynein light chain (10 kDa, Protein from Tetrahymena thermophila CU428)
- Dynein light chain (13 kDa, Protein from Tetrahymena thermophila CU428)
- Outer dynein arm complex from tetrahymena thermophila (2 MDa, Cellular component from Tetrahymena thermophila CU428)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Tubulin alpha chain (49 kDa, Protein from Tetrahymena thermophila CU428)
- Tubulin beta chain (49 kDa, Protein from Tetrahymena thermophila CU428)
- Dynein light chain 2a (15 kDa, Protein from Tetrahymena thermophila CU428)
- Dynein light chain roadblock-type 2 protein (18 kDa, Protein from Tetrahymena thermophila CU428)
- Dynein light chain (12 kDa, Protein from Tetrahymena thermophila CU428)
- Flagellar outer dynein arm intermediate protein, putative (77 kDa, Protein from Tetrahymena thermophila CU428)
- Outer dynein arm docking complex protein oda protein (35 kDa, Protein from Tetrahymena thermophila CU428)
- Outer dynein arm docking complex protein oda protein (63 kDa, Protein from Tetrahymena thermophila CU428)
- Outer arm dynein beta heavy chain (530 kDa, Protein from Tetrahymena thermophila CU428)
- Docking complex 1/2 protein (11 kDa, Protein from Tetrahymena thermophila CU428)
- Dynein light chain (12 kDa, Protein from Tetrahymena thermophila CU428)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Dynein light chain (10 kDa, Protein from Tetrahymena thermophila CU428)
- Dynein heavy chain, outer arm protein (534 kDa, Protein from Tetrahymena thermophila CU428)
- Dynein light chain (10 kDa, Protein from Tetrahymena thermophila CU428)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Thioredoxin (12 kDa, Protein from Tetrahymena thermophila CU428)
- Dynein light chain roadblock-type 2 protein (14 kDa, Protein from Tetrahymena thermophila CU428)
- Dynein light chain tctex-type 1 protein (13 kDa, Protein from Tetrahymena thermophila CU428)
- Dynein intermediate chain 2 (77 kDa, Protein from Tetrahymena thermophila CU428)
Outer Dynein Arm-Shulin complex - Dyh3 motor region (Tetrahymena thermophila)
Resolution: 4.4 Å
EM Method: Single-particle
Fitted PDBs: 6zyy
Q-score: 0.388
Mali GR, Ali FA, Lau CK, Begum F, Boulanger J, Howe JD, Chen ZA, Rappsilber J, Skehel M, Carter AP
Science (2021) 371 pp. 910-916 [ DOI: doi:10.1126/science.abe0526 Pubmed: 33632841 ]
- Dynein heavy chain, outer arm protein (534 kDa, Protein from Tetrahymena thermophila (strain SB210))
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Shulin (139 kDa, Protein from Tetrahymena thermophila (strain SB210))
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Tetrahymena thermophila oda dyh3 motor region (includes shulin c3 finger) (1 MDa, Complex from Tetrahymena thermophila)
Outer Dynein Arm-Shulin complex - overall structure (Tetrahymena thermophila)
Resolution: 8.78 Å
EM Method: Single-particle
Fitted PDBs: 6zyw
Q-score: 0.103
Mali GR, Ali FA, Lau CK, Begum F, Boulanger J, Howe JD, Chen ZA, Rappsilber J, Skehel M, Carter AP
Science (2021) 371 pp. 910-916 [ DOI: doi:10.1126/science.abe0526 Pubmed: 33632841 ]
- Outer arm dynein beta heavy chain (530 kDa, Protein from Tetrahymena thermophila SB210)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Tetrahymena thermophila oda dyh4 motor (1 MDa, Complex)
- Dynein light chain roadblock-type 2 protein (14 kDa, Protein from Tetrahymena thermophila SB210)
- Dynein heavy chain, outer arm protein (534 kDa, Protein from Tetrahymena thermophila SB210)
- Flagellar outer dynein arm intermediate protein, putative (77 kDa, Protein from Tetrahymena thermophila SB210)
- Dynein intermediate chain 2 (77 kDa, Protein from Tetrahymena thermophila SB210)
- Dynein light chain (10 kDa, Protein from Tetrahymena thermophila SB210)
- Dynein light chain (12 kDa, Protein from Tetrahymena thermophila SB210)
- Dynein light chain 2a (15 kDa, Protein from Tetrahymena thermophila SB210)
- Dynein-1-alpha heavy chain, flagellar inner arm i1 complex protein, putative (475 kDa, Protein from Tetrahymena thermophila SB210)
- Shulin (Complex from Tetrahymena thermophila (strain SB210))
- Dynein light chain (10 kDa, Protein from Tetrahymena thermophila SB210)
- Tetrahymena thermophila oda dyh4 motor (Complex from Tetrahymena thermophila (strain SB210))
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Dynein light chain (13 kDa, Protein from Tetrahymena thermophila SB210)
- Dynein light chain roadblock-type 2 protein (11 kDa, Protein from Tetrahymena thermophila SB210)
- Dynein light chain (10 kDa, Protein from Tetrahymena thermophila SB210)
- Dynein light chain tctex-type 1 protein (13 kDa, Protein from Tetrahymena thermophila SB210)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Thioredoxin (12 kDa, Protein from Tetrahymena thermophila SB210)
- Dynein light chain (12 kDa, Protein from Tetrahymena thermophila SB210)
- Shulin (139 kDa, Protein from Tetrahymena thermophila SB210)
Outer Dynein Arm-Shulin complex - Shulin region from Tetrahymena thermophila
Resolution: 4.3 Å
EM Method: Single-particle
Fitted PDBs: 6zyx
Q-score: 0.398
Mali GR, Ali FA, Lau CK, Begum F, Boulanger J, Howe JD, Chen ZA, Rappsilber J, Skehel M, Carter AP
Science (2021) 371 pp. 910-916 [ DOI: doi:10.1126/science.abe0526 Pubmed: 33632841 ]
- Dynein (Complex from Tetrahymena thermophila CU428)
- Shulin (Complex from Tetrahymena thermophila CU428)
- Dynein light chain (10 kDa, Protein from Tetrahymena thermophila CU428)
- Dynein light chain (12 kDa, Protein from Tetrahymena thermophila CU428)
- Flagellar outer dynein arm intermediate protein, putative (77 kDa, Protein from Tetrahymena thermophila CU428)
- Dynein heavy chain, outer arm protein (534 kDa, Protein from Tetrahymena thermophila CU428)
- Dynein light chain (10 kDa, Protein from Tetrahymena thermophila CU428)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Shulin region from outer dynein arm-shulin complex (tetrahymena thermophila). contains shulin (excluding c3 finger), lc8e, lc8f, lc8d, lc8, lc2a, lc9, dic n-termini and portion of dyh3 tail. (Complex)
- Dynein light chain (13 kDa, Protein from Tetrahymena thermophila CU428)
- Shulin (139 kDa, Protein from Tetrahymena thermophila CU428)
- Dynein light chain tctex-type 1 protein (13 kDa, Protein from Tetrahymena thermophila CU428)
- Dynein light chain 2a (15 kDa, Protein from Tetrahymena thermophila CU428)
- Dynein intermediate chain 2 (77 kDa, Protein from Tetrahymena thermophila CU428)
Complex of yeast cytoplasmic dynein MTBD-High and MT without DTT
Resolution: 3.94 Å
EM Method: Helical reconstruction
Fitted PDBs: 6kio
Q-score: 0.414
Nishida N, Komori Y, Takarada O, Watanabe A, Tamura S, Kubo S, Shimada I, Kikkawa M
Nat Commun (2020) 11 pp. 1038-1038 [ DOI: doi:10.1038/s41467-020-14842-8 Pubmed: 32098965 ]
- Dynein heavy chain, cytoplasmic (15 kDa, Protein from Saccharomyces cerevisiae S288c)
- Tubulin alpha-1a chain (45 kDa, Protein from Sus scrofa)
- Mtbd-high/mt complex without dtt (Cell from Sus scrofa)
- Tubulin beta chain (47 kDa, Protein from Sus scrofa)
Complex of yeast cytoplasmic dynein MTBD-High and MT with DTT
Resolution: 3.62 Å
EM Method: Helical reconstruction
Fitted PDBs: 6kiq
Q-score: 0.455
Nishida N, Komori Y, Takarada O, Watanabe A, Tamura S, Kubo S, Shimada I, Kikkawa M
Nat Commun (2020) 11 pp. 1038-1038 [ DOI: doi:10.1038/s41467-020-14842-8 Pubmed: 32098965 ]
- Alpha tubulin (45 kDa, Protein from Sus scrofa)
- Dynein heavy chain, cytoplasmic (15 kDa, Protein from Saccharomyces cerevisiae S288c)
- Mtbd-high/mt complex with dtt (Cell from Sus scrofa)
- Tubulin beta chain (47 kDa, Protein from Sus scrofa)
Resolution: 4.5 Å
EM Method: Single-particle
Fitted PDBs: 6rza
Q-score: 0.228
Lacey SE, He S, Scheres SH, Carter AP
eLife (2019) 8 [ DOI: doi:10.7554/eLife.47145 Pubmed: 31264960 ]
- Magnesium ion (24 Da, Ligand)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Tubulin beta chain (47 kDa, Protein from Sus scrofa)
- Tubulin alpha-1b chain (48 kDa, Protein from Sus scrofa)
- Taxol (853 Da, Ligand)
- Dnah7 (Complex from Homo sapiens)
- Dnah7 microtubule binding domain bound to microtubules (Complex)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Tubulin (Complex from Sus scrofa)
- Cytoplasmic dynein 1 heavy chain 1,dynein heavy chain 7, axonemal,cytoplasmic dynein 1 heavy chain 1 (18 kDa, Protein from Mus musculus)
Cryo-EM structure of two dynein tail domains bound to dynactin and BICDR1
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 6f1t
Q-score: 0.269
Urnavicius L, Lau CK, Elshenawy MM, Morales-Rios E, Motz C, Yildiz A, Carter AP
Nature (2018) 554 pp. 202-206 [ Pubmed: 29420470 DOI: doi:10.1038/nature25462 ]
- Dynactin subunit 1 (4 kDa, Protein from Sus scrofa)
- Bicd family-like cargo adapter 1,bicd family-like cargo adapter 1,bicd family-like cargo adapter 1,bicdr-1 (42 kDa, Protein from Mus musculus)
- Dynactin subunit 3 (5 kDa, Protein from Sus scrofa)
- Dynactin subunit 2 (10 kDa, Protein from Sus scrofa)
- Cytoplasmic dynein 1 intermediate chain 2 (68 kDa, Protein from Homo sapiens)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Dynactin subunit 1 (4 kDa, Protein from Sus scrofa)
- Cryo-em map of two dynein tail domains bound to dynactin and bicdr1 (Complex)
- Dynactin subunit 2 (5 kDa, Protein from Sus scrofa)
- Cytoplasmic dynein 1 light intermediate chain 2 (54 kDa, Protein from Homo sapiens)
- Dynactin subunit 2 (2 kDa, Protein from Sus scrofa)
- Bicd family-like cargo adapter 1 (Complex from Mus musculus)
- Dynactin subunit 5 (20 kDa, Protein from Sus scrofa)
- Capping protein (actin filament) muscle z-line, alpha 1 (33 kDa, Protein from Sus scrofa)
- Arp1 actin related protein 1 homolog a (42 kDa, Protein from Sus scrofa)
- Dynactin (Complex from Sus scrofa)
- Dynactin subunit 2 (52 kDa, Protein from Sus scrofa)
- Dynactin subunit 4 (22 kDa, Protein from Sus scrofa)
- Dynein light chain roadblock-type 1 (10 kDa, Protein from Homo sapiens)
- Dynactin subunit 2 (7 kDa, Protein from Sus scrofa)
- Dynactin subunit 2 (7 kDa, Protein from Sus scrofa)
- Bicd family-like cargo adapter 1,bicd family-like cargo adapter 1,bicd family-like cargo adapter 1 (42 kDa, Protein from Mus musculus)
- Cytoplasmic dynein 1 heavy chain 1,cytoplasmic dynein 1 heavy chain 1,cytoplasmic dynein 1 heavy chain 1,dynein heavy chain,cytoplasmic dynein 1 heavy chain 1,cytoplasmic dynein 1 heavy chain 1,cytoplasmic dynein 1 heavy chain 1,cytoplasmic dynein 1 heavy chain 1,dynein heavy chain,cytoplasmic dynein 1 heavy chain 1,cytoplasmic dynein 1 heavy chain 1 (119 kDa, Protein from Homo sapiens)
- Actin, cytoplasmic 1 (41 kDa, Protein from Sus scrofa)
- F-actin capping protein beta subunit (30 kDa, Protein from Sus scrofa)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Dynein (Complex from Homo sapiens)
- Dynactin subunit 2 (50 kDa, Protein from Sus scrofa)
- Dynactin 6 (20 kDa, Protein from Sus scrofa)
- Actin related protein 10 homolog (43 kDa, Protein from Sus scrofa)