70S ribosome complex with dnaX mRNA stemloop and E-site tRNA ("in" conformation)
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 5uq7
Q-score: 0.397
Zhang Y, Hong S, Ruangprasert A, Skiniotis G, Dunham CM
Structure (2018) 26 pp. 437-445.e3 [ Pubmed: 29456023 DOI: doi:10.1016/j.str.2018.01.013 ]
- 50s ribosomal protein l21 (11 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s4 (24 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Iron/sulfur cluster (351 Da, Ligand)
- 50s ribosomal protein l19 (15 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s7 (17 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l17 (13 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l34 (5 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s8 (15 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l24 (11 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l14 (13 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- E-site trna phe (24 kDa, RNA from Escherichia coli K-12)
- Messenger rna (17 kDa, RNA from synthetic construct)
- 50s ribosomal protein l30 (6 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 16s rrna (493 kDa, RNA from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l32 (6 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s16 (9 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l36 (4 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s15 (10 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l23 (10 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 23s rrna (948 kDa, RNA from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s14 type z (7 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l35 (7 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l22 (12 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 70s-dnax complex (Complex from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l27 (8 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Zinc ion (65 Da, Ligand)
- 50s ribosomal protein l13 (15 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l18 (12 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l28 (10 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s3 (22 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l4 (22 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l25 (21 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l2 (30 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s17 (11 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s10 (11 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l3 (22 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l15 (16 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s20 (10 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s9 (14 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l20 (13 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein thx (2 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l29 (8 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l5 (20 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 5s rrna (38 kDa, RNA from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s12 (13 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s13 (13 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l1 (24 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s18 (7 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l33 (6 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- P-site trna fmet (24 kDa, RNA from Escherichia coli K-12)
- 30s ribosomal protein s2 (26 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s6 (11 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l9 (16 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s11 (12 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s19 (9 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l31 (8 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s5 (16 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l6 (18 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l16 (15 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
70S ribosome complex with dnaX mRNA stem-loop and E-site tRNA ("out" conformation)
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 5uq8
Q-score: 0.446
Zhang Y, Hong S, Ruangprasert A, Skiniotis G, Dunham CM
Structure (2018) 26 pp. 437-445.e3 [ Pubmed: 29456023 DOI: doi:10.1016/j.str.2018.01.013 ]
- 50s ribosomal protein l21 (11 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s4 (24 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Iron/sulfur cluster (351 Da, Ligand)
- 50s ribosomal protein l19 (15 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s7 (17 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l17 (13 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l34 (5 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s8 (15 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l24 (11 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l14 (13 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- E-site trna phe (24 kDa, RNA from Escherichia coli K-12)
- Messenger rna (17 kDa, RNA from synthetic construct)
- 50s ribosomal protein l30 (6 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 16s rrna (493 kDa, RNA from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l32 (6 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s16 (9 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l36 (4 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s15 (10 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l23 (10 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 23s rrna (948 kDa, RNA from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s14 type z (7 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l35 (7 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l22 (12 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 70s-dnax complex (Complex from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l27 (8 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Zinc ion (65 Da, Ligand)
- 50s ribosomal protein l13 (15 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l18 (12 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l28 (10 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s3 (22 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l4 (22 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l25 (21 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l2 (30 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s17 (11 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s10 (11 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l3 (22 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l15 (16 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s20 (10 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s9 (14 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l20 (13 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein thx (2 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l29 (8 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l5 (20 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 5s rrna (38 kDa, RNA from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s12 (13 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s13 (13 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l1 (24 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s18 (7 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l33 (6 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- P-site trna fmet (24 kDa, RNA from Escherichia coli K-12)
- 30s ribosomal protein s2 (26 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s6 (11 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l9 (16 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s11 (12 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s19 (9 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l31 (8 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s5 (16 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l6 (18 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l16 (15 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
Structure of RelA bound to the 70S ribosome (overall map, class 1)
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 5iqr
Q-score: 0.45
Brown A, Fernandez IS, Gordiyenko Y, Ramakrishnan V
Nature (2016) 534 pp. 277-280 [ Pubmed: 27279228 DOI: doi:10.1038/nature17675 ]
- 50s ribosomal protein l11 (14 kDa, Protein from Escherichia coli K-12)
- Gtp pyrophosphokinase (83 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s3 (25 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s8 (14 kDa, Protein from Escherichia coli K-12)
- E-site trna(phe) (24 kDa, RNA from Escherichia coli K-12)
- 30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli K-12)
- Ssu rrna (497 kDa, RNA from Escherichia coli K-12)
- 50s ribosomal protein l19 (13 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s14 (11 kDa, Protein from Escherichia coli K-12)
- 5s rrna (38 kDa, RNA from Escherichia coli K-12)
- 50s ribosomal protein l22 (12 kDa, Protein from Escherichia coli K-12)
- Ribosome (Complex from Escherichia coli K-12)
- 50s ribosomal protein l35 (7 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l33 (6 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l10 (17 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s5 (17 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l2 (29 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l28 (9 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l13 (16 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l21 (11 kDa, Protein from Escherichia coli K-12)
- Water (18 Da, Ligand)
- 50s ribosomal protein l9 (15 kDa, Protein from Escherichia coli K-12)
- P-site fmet-trna(fmet) (24 kDa, RNA from Escherichia coli K-12)
- Zinc ion (65 Da, Ligand)
- Mrna (3 kDa, RNA from synthetic construct)
- 30s ribosomal protein s7 (20 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l23 (11 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l4 (22 kDa, Protein from Escherichia coli K-12)
- A/t trna(phe) (24 kDa, RNA from Escherichia coli K-12)
- 50s ribosomal protein l30 (6 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l24 (11 kDa, Protein from Escherichia coli K-12)
- Magnesium ion (24 Da, Ligand)
- 50s ribosomal protein l16 (15 kDa, Protein from Escherichia coli K-12)
- Paromomycin (615 Da, Ligand)
- 30s ribosomal protein s11 (13 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s17 (9 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l17 (14 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s2 (26 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s9 (14 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l3 (22 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s6 (15 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s13 (13 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l32 (6 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s18 (9 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s21 (8 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l31 (7 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l25 (10 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s15 (10 kDa, Protein from Escherichia coli K-12)
- Methionine (149 Da, Ligand)
- Lsu rrna (941 kDa, RNA from Escherichia coli K-12)
- 30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s19 (10 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l20 (13 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l27 (9 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l36 (4 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l5 (20 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l15 (15 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s20 (9 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l14 (13 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l34 (5 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l6 (18 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s4 (23 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s12 (13 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l29 (7 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l18 (12 kDa, Protein from Escherichia coli K-12)