Cryo-EM structure of human DICER-pre-miRNA in a dicing state
Resolution: 3.04 Å
EM Method: Single-particle
Fitted PDBs: 7xw2
Q-score: 0.444
Lee YY, Lee H, Kim H, Kim VN, Roh SH
Nature (2023) 615 pp. 331-338 [ Pubmed: 36813958 DOI: doi:10.1038/s41586-023-05723-3 ]
- Calcium ion (40 Da, Ligand)
- Pre-mirna (Complex)
- Rna (73-mer) (23 kDa, RNA from Homo sapiens)
- Endoribonuclease dicer (218 kDa, Protein from Homo sapiens)
- Human dicer (Complex from Homo sapiens)
- Protein-rna complex of human dicer with pre-mirna (Complex)
Cryo-EM structure of an apo-form of human DICER
Resolution: 4.04 Å
EM Method: Single-particle
Fitted PDBs: 7xw3
Q-score: 0.285
Lee YY, Lee H, Kim H, Kim VN, Roh SH
Nature (2023) 615 pp. 331-338 [ Pubmed: 36813958 DOI: doi:10.1038/s41586-023-05723-3 ]
- Endoribonuclease dicer (218 kDa, Protein from Homo sapiens)
- Monomeric form of human dicer (Complex from Homo sapiens)
Mammalian Dicer in the "pre-dicing state" with pre-miR-15a substrate and TARBP2 subunit
Resolution: 3.81 Å
EM Method: Single-particle
Fitted PDBs: 7zpj
Q-score: -0.01
Zapletal D, Taborska E, Pasulka J, Malik R, Kubicek K, Zanova M, Much C, Sebesta M, Buccheri V, Horvat F, Jenickova I, Prochazkova M, Prochazka J, Pinkas M, Novacek J, Joseph DF, Sedlacek R, Bernecky C, O'Carroll D, Stefl R, Svoboda P
Mol Cell (2022) 82 pp. 4064-4079.e13 [ DOI: doi:10.1016/j.molcel.2022.10.010 Pubmed: 36332606 ]
- Pre-dicing state of mouse somatic dicer with pre-mir-15a and tarbp2 (Complex)
- Risc-loading complex subunit tarbp2 isoform 1 (39 kDa, Protein from Mus musculus)
- 59-nt precursor of mir-15a (19 kDa, RNA from Mus musculus)
- Endoribonuclease dicer (226 kDa, Protein from Mus musculus)
- Pre-mir-15a (Complex from Mus musculus)
- Pre-dicing state of mouse somatic dicer with tarbp2 (Complex from Mus musculus)
Mammalian Dicer in the "pre-dicing state" with pre-miR-15a substrate
Resolution: 4.19 Å
EM Method: Single-particle
Fitted PDBs: 7yym
Q-score: 0.192
Zapletal D, Taborska E, Pasulka J, Malik R, Kubicek K, Zanova M, Much C, Sebesta M, Buccheri V, Horvat F, Jenickova I, Prochazkova M, Prochazka J, Pinkas M, Novacek J, Joseph DF, Sedlacek R, Bernecky C, O'Carroll D, Stefl R, Svoboda P
Mol Cell (2022) 82 pp. 4064-4079.e13 [ DOI: doi:10.1016/j.molcel.2022.10.010 Pubmed: 36332606 ]
- Endoribonuclease dicer (226 kDa, Protein from Mus musculus)
- Pre-mir-15a (Complex from synthetic construct)
- 59-nt precursor of mir-15a (19 kDa, RNA from synthetic construct)
- Pre-dicing state of mouse somatic dicer (Complex from Mus musculus)
- Pre-dicing state of mouse somatic dicer with pre-mir-15a (Complex)
Mammalian Dicer in the dicing state with pre-miR-15a substrate
Resolution: 6.21 Å
EM Method: Single-particle
Fitted PDBs: 7yyn
Q-score: 0.133
Zapletal D, Taborska E, Pasulka J, Malik R, Kubicek K, Zanova M, Much C, Sebesta M, Buccheri V, Horvat F, Jenickova I, Prochazkova M, Prochazka J, Pinkas M, Novacek J, Joseph DF, Sedlacek R, Bernecky C, O'Carroll D, Stefl R, Svoboda P
Mol Cell (2022) 82 pp. 4064-4079.e13 [ DOI: doi:10.1016/j.molcel.2022.10.010 Pubmed: 36332606 ]
- Isoform 2 of endoribonuclease dicer (200 kDa, Protein from Mus musculus)
- 59-nt precursor of mir-15a (Complex from Mus musculus)
- Isoform 2 of endoribonuclease dicer (Complex from Mus musculus)
- 59-nt precursor of mir-15a (19 kDa, RNA from Mus musculus)
- Dicing state of mouse oocyte dicer with pre-mir-15a (Complex)
Mouse endoribonuclease Dicer (composite structure)
Resolution: 3.84 Å
EM Method: Single-particle
Fitted PDBs: 7yz4
Q-score: 0.293
Zapletal D, Taborska E, Pasulka J, Malik R, Kubicek K, Zanova M, Much C, Sebesta M, Buccheri V, Horvat F, Jenickova I, Prochazkova M, Prochazka J, Pinkas M, Novacek J, Joseph DF, Sedlacek R, Bernecky C, O'Carroll D, Stefl R, Svoboda P
Mol Cell (2022) 82 pp. 4064-4079.e13 [ DOI: doi:10.1016/j.molcel.2022.10.010 Pubmed: 36332606 ]
- Free form of mouse somatic dicer (Cellular component from Mus musculus)
- Endoribonuclease dicer (226 kDa, Protein from Mus musculus)
Mammalian Dicer in the "dicing state" with pre-miR-15a substrate
Resolution: 5.91 Å
EM Method: Single-particle
Fitted PDBs: 7zpi
Q-score: 0.106
Zapletal D, Taborska E, Pasulka J, Malik R, Kubicek K, Zanova M, Much C, Sebesta M, Buccheri V, Horvat F, Jenickova I, Prochazkova M, Prochazka J, Pinkas M, Novacek J, Joseph DF, Sedlacek R, Bernecky C, O'Carroll D, Stefl R, Svoboda P
Mol Cell (2022) 82 pp. 4064-4079.e13 [ DOI: doi:10.1016/j.molcel.2022.10.010 Pubmed: 36332606 ]
- Dicing state of mouse somatic dicer (Complex from Mus musculus)
- 59-nt precursor of mir-15a (19 kDa, RNA from Mus musculus)
- Endoribonuclease dicer (226 kDa, Protein from Mus musculus)
- Dicing state of mouse somatic dicer with pre-mir-15a (Complex)
- Pre-mir-15a (Complex from Mus musculus)
Mammalian Dicer in the "pre-dicing state" with pre-miR-15a substrate and TARBP2 subunit
Resolution: 3.81 Å
EM Method: Single-particle
Fitted PDBs: 7zpk
Q-score: 0.112
Zapletal D, Taborska E, Pasulka J, Malik R, Kubicek K, Zanova M, Much C, Sebesta M, Buccheri V, Horvat F, Jenickova I, Prochazkova M, Prochazka J, Pinkas M, Novacek J, Joseph DF, Sedlacek R, Bernecky C, O'Carroll D, Stefl R, Svoboda P
Mol Cell (2022) 82 pp. 4064-4079.e13 [ DOI: doi:10.1016/j.molcel.2022.10.010 Pubmed: 36332606 ]
- Pre-dicing state of mouse somatic dicer with pre-mir-15a and tarbp2 (Complex)
- 59-nt precursor of mir-15a (19 kDa, RNA from Mus musculus)
- Endoribonuclease dicer (226 kDa, Protein from Mus musculus)
- Risc-loading complex subunit tarbp2 (39 kDa, Protein from Mus musculus)
- Pre-mir-15a (Complex from Mus musculus)
- Pre-dicing state of mouse somatic dicer with tarbp2 (Complex from Mus musculus)
Cryo-EM structure of Arabidopsis DCL1 in complex with pri-miRNA 166f
Resolution: 4.6 Å
EM Method: Single-particle
Fitted PDBs: 7eld
Q-score: 0.157
Wei X, Ke H, Wen A, Gao B, Shi J, Feng Y
Nat Plants (2021) 7 pp. 1389-1396 [ DOI: doi:10.1038/s41477-021-01000-1 Pubmed: 34593993 ]
- Pri-mirna 166f (47 kDa, RNA from Arabidopsis thaliana)
- Endoribonuclease dicer homolog 1 (213 kDa, Protein from Arabidopsis thaliana)
- Binary complex of dcl1 in complex with pri-mirna 166f (Complex from Arabidopsis thaliana)
Cryo-EM structure of Arabidopsis DCL1 in complex with pre-miRNA 166f
Resolution: 4.9 Å
EM Method: Single-particle
Fitted PDBs: 7ele
Q-score: 0.186
Wei X, Ke H, Wen A, Gao B, Shi J, Feng Y
Nat Plants (2021) 7 pp. 1389-1396 [ DOI: doi:10.1038/s41477-021-01000-1 Pubmed: 34593993 ]
- Endoribonuclease dicer homolog 1 (213 kDa, Protein from Arabidopsis thaliana)
- Pre-mirna 166f (28 kDa, RNA from Arabidopsis thaliana)
- Binary complex of dcl1 in complex with pre-mirna 166f (Complex from Arabidopsis thaliana)