Cryo-EM structure of human DICER-pre-miRNA in a dicing state
Resolution: 3.04 Å
EM Method: Single-particle
Fitted PDBs: 7xw2
Q-score: 0.444
Lee YY, Lee H, Kim H, Kim VN, Roh SH
Nature (2023) 615 pp. 331-338 [ DOI: doi:10.1038/s41586-023-05723-3 Pubmed: 36813958 ]
- Human dicer (Complex from Homo sapiens)
- Rna (73-mer) (23 kDa, RNA from Homo sapiens)
- Pre-mirna (Complex)
- Protein-rna complex of human dicer with pre-mirna (Complex)
- Calcium ion (40 Da, Ligand)
- Endoribonuclease dicer (218 kDa, Protein from Homo sapiens)
Mammalian Dicer in the "pre-dicing state" with pre-miR-15a substrate and TARBP2 subunit
Resolution: 3.81 Å
EM Method: Single-particle
Fitted PDBs: 7zpj
Q-score: -0.01
Zapletal D, Taborska E, Pasulka J, Malik R, Kubicek K, Zanova M, Much C, Sebesta M, Buccheri V, Horvat F, Jenickova I, Prochazkova M, Prochazka J, Pinkas M, Novacek J, Joseph DF, Sedlacek R, Bernecky C, O'Carroll D, Stefl R, Svoboda P
Mol Cell (2022) 82 pp. 4064-4079.e13 [ DOI: doi:10.1016/j.molcel.2022.10.010 Pubmed: 36332606 ]
- Endoribonuclease dicer (226 kDa, Protein from Mus musculus)
- Pre-mir-15a (Complex from Mus musculus)
- Risc-loading complex subunit tarbp2 isoform 1 (39 kDa, Protein from Mus musculus)
- 59-nt precursor of mir-15a (19 kDa, RNA from Mus musculus)
- Pre-dicing state of mouse somatic dicer with tarbp2 (Complex from Mus musculus)
- Pre-dicing state of mouse somatic dicer with pre-mir-15a and tarbp2 (Complex)
Mammalian Dicer in the "pre-dicing state" with pre-miR-15a substrate
Resolution: 4.19 Å
EM Method: Single-particle
Fitted PDBs: 7yym
Q-score: 0.192
Zapletal D, Taborska E, Pasulka J, Malik R, Kubicek K, Zanova M, Much C, Sebesta M, Buccheri V, Horvat F, Jenickova I, Prochazkova M, Prochazka J, Pinkas M, Novacek J, Joseph DF, Sedlacek R, Bernecky C, O'Carroll D, Stefl R, Svoboda P
Mol Cell (2022) 82 pp. 4064-4079.e13 [ DOI: doi:10.1016/j.molcel.2022.10.010 Pubmed: 36332606 ]
- 59-nt precursor of mir-15a (19 kDa, RNA from synthetic construct)
- Pre-dicing state of mouse somatic dicer (Complex from Mus musculus)
- Pre-dicing state of mouse somatic dicer with pre-mir-15a (Complex)
- Pre-mir-15a (Complex from synthetic construct)
- Endoribonuclease dicer (226 kDa, Protein from Mus musculus)
Mammalian Dicer in the dicing state with pre-miR-15a substrate
Resolution: 6.21 Å
EM Method: Single-particle
Fitted PDBs: 7yyn
Q-score: 0.133
Zapletal D, Taborska E, Pasulka J, Malik R, Kubicek K, Zanova M, Much C, Sebesta M, Buccheri V, Horvat F, Jenickova I, Prochazkova M, Prochazka J, Pinkas M, Novacek J, Joseph DF, Sedlacek R, Bernecky C, O'Carroll D, Stefl R, Svoboda P
Mol Cell (2022) 82 pp. 4064-4079.e13 [ DOI: doi:10.1016/j.molcel.2022.10.010 Pubmed: 36332606 ]
- Dicing state of mouse oocyte dicer with pre-mir-15a (Complex)
- Isoform 2 of endoribonuclease dicer (Complex from Mus musculus)
- 59-nt precursor of mir-15a (19 kDa, RNA from Mus musculus)
- 59-nt precursor of mir-15a (Complex from Mus musculus)
- Isoform 2 of endoribonuclease dicer (200 kDa, Protein from Mus musculus)
Mammalian Dicer in the "dicing state" with pre-miR-15a substrate
Resolution: 5.91 Å
EM Method: Single-particle
Fitted PDBs: 7zpi
Q-score: 0.106
Zapletal D, Taborska E, Pasulka J, Malik R, Kubicek K, Zanova M, Much C, Sebesta M, Buccheri V, Horvat F, Jenickova I, Prochazkova M, Prochazka J, Pinkas M, Novacek J, Joseph DF, Sedlacek R, Bernecky C, O'Carroll D, Stefl R, Svoboda P
Mol Cell (2022) 82 pp. 4064-4079.e13 [ DOI: doi:10.1016/j.molcel.2022.10.010 Pubmed: 36332606 ]
- Endoribonuclease dicer (226 kDa, Protein from Mus musculus)
- Pre-mir-15a (Complex from Mus musculus)
- Dicing state of mouse somatic dicer (Complex from Mus musculus)
- 59-nt precursor of mir-15a (19 kDa, RNA from Mus musculus)
- Dicing state of mouse somatic dicer with pre-mir-15a (Complex)
Mammalian Dicer in the "pre-dicing state" with pre-miR-15a substrate and TARBP2 subunit
Resolution: 3.81 Å
EM Method: Single-particle
Fitted PDBs: 7zpk
Q-score: 0.112
Zapletal D, Taborska E, Pasulka J, Malik R, Kubicek K, Zanova M, Much C, Sebesta M, Buccheri V, Horvat F, Jenickova I, Prochazkova M, Prochazka J, Pinkas M, Novacek J, Joseph DF, Sedlacek R, Bernecky C, O'Carroll D, Stefl R, Svoboda P
Mol Cell (2022) 82 pp. 4064-4079.e13 [ DOI: doi:10.1016/j.molcel.2022.10.010 Pubmed: 36332606 ]
- Risc-loading complex subunit tarbp2 (39 kDa, Protein from Mus musculus)
- Endoribonuclease dicer (226 kDa, Protein from Mus musculus)
- Pre-mir-15a (Complex from Mus musculus)
- 59-nt precursor of mir-15a (19 kDa, RNA from Mus musculus)
- Pre-dicing state of mouse somatic dicer with tarbp2 (Complex from Mus musculus)
- Pre-dicing state of mouse somatic dicer with pre-mir-15a and tarbp2 (Complex)
Cryo-EM structure of Arabidopsis DCL1 in complex with pri-miRNA 166f
Resolution: 4.6 Å
EM Method: Single-particle
Fitted PDBs: 7eld
Q-score: 0.157
Wei X, Ke H, Wen A, Gao B, Shi J, Feng Y
Nat Plants (2021) 7 pp. 1389-1396 [ Pubmed: 34593993 DOI: doi:10.1038/s41477-021-01000-1 ]
- Binary complex of dcl1 in complex with pri-mirna 166f (Complex from Arabidopsis thaliana)
- Endoribonuclease dicer homolog 1 (213 kDa, Protein from Arabidopsis thaliana)
- Pri-mirna 166f (47 kDa, RNA from Arabidopsis thaliana)
Cryo-EM structure of Arabidopsis DCL1 in complex with pre-miRNA 166f
Resolution: 4.9 Å
EM Method: Single-particle
Fitted PDBs: 7ele
Q-score: 0.186
Wei X, Ke H, Wen A, Gao B, Shi J, Feng Y
Nat Plants (2021) 7 pp. 1389-1396 [ Pubmed: 34593993 DOI: doi:10.1038/s41477-021-01000-1 ]
- Binary complex of dcl1 in complex with pre-mirna 166f (Complex from Arabidopsis thaliana)
- Endoribonuclease dicer homolog 1 (213 kDa, Protein from Arabidopsis thaliana)
- Pre-mirna 166f (28 kDa, RNA from Arabidopsis thaliana)
Cryo-EM structure of human Dicer and its complexes with a pre-miRNA substrate
Resolution: 4.7 Å
EM Method: Single-particle
Fitted PDBs: 5zal
Q-score: 0.216
Liu Z, Wang J, Cheng H, Ke X, Sun L, Zhang QC, Wang HW
Cell (2018) 173 pp. 1191-1203.e12 [ DOI: doi:10.1016/j.cell.2018.03.080 Pubmed: 29706542 ]
- Human dicer-trbp with pre-let-7 complex class i (Complex from Homo sapiens)
- Endoribonuclease dicer (218 kDa, Protein from Homo sapiens)
- Dicer and trbp (270 kDa, Complex from Homo sapiens)
- Rna (73-mer) (23 kDa, RNA from Homo sapiens)
- Risc-loading complex subunit tarbp2 (39 kDa, Protein from Homo sapiens)
Cryo-EM structure of human Dicer and its complexes with a pre-miRNA substrate
Resolution: 5.7 Å
EM Method: Single-particle
Fitted PDBs: 5zam
Q-score: 0.215
Liu Z, Wang J, Cheng H, Ke X, Sun L, Zhang QC, Wang HW
Cell (2018) 173 pp. 1191-1203.e12 [ DOI: doi:10.1016/j.cell.2018.03.080 Pubmed: 29706542 ]
- Endoribonuclease dicer (218 kDa, Protein from Homo sapiens)
- Dicer and trbp (270 kDa, Complex from Homo sapiens)
- Rna (73-mer) (23 kDa, RNA from Homo sapiens)
- Risc-loading complex subunit tarbp2 (39 kDa, Protein from Homo sapiens)
- Human dicer-trbp with pre-let-7 complex class ii (Complex from Homo sapiens)