Resolution: 4.0 Å
EM Method: Single-particle
Fitted PDBs: 8s8k
Q-score: 0.318
Villamayor-Belinchon L, Sharma P, Gordiyenko Y, Llacer JL, Hussain T
Nucleic Acids Res (2024) 52 pp. 11317-11335 [ DOI: doi:10.1093/nar/gkae737 Pubmed: 39193907 ]
- Eukaryotic translation initiation factor 1a (17 kDa, Protein from Saccharomyces cerevisiae S288C)
- Eukaryotic translation initiation factor 3 subunit c (93 kDa, Protein from Saccharomyces cerevisiae S288C)
- Eukaryotic translation initiation factor 2 subunit gamma (57 kDa, Protein from Saccharomyces cerevisiae S288C)
- Eukaryotic translation initiation factor 2 subunit beta (29 kDa, Protein from Saccharomyces cerevisiae S288C)
- 40s ribosomal protein s27 (8 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Klla0e23673p (21 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Mrna (Complex from Kluyveromyces lactis)
- Methionine (149 Da, Ligand)
- Small ribosomal subunit protein us10 (13 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Initiation factor eif3 (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Klla0a10483p (17 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Eukaryotic translation initiation factor 2 subunit alpha (34 kDa, Protein from Saccharomyces cerevisiae S288C)
- Small ribosomal subunit protein us8 (14 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Ribosome (Complex from Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37))
- 40s ribosomal protein s8 (22 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Initiation factor eif1a (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s3 (26 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Trna (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Klla0b01474p (15 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Klla0f07843p (15 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Klla0b01562p (17 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Small ribosomal subunit protein es6 (26 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Phosphomethylphosphonic acid guanylate ester (521 Da, Ligand)
- 40s ribosomal protein s21 (9 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Magnesium ion (24 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Klla0b11231p (16 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- 40s ribosomal protein s4 (29 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Initiation factor eif2 (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s30 (7 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Small ribosomal subunit protein us2 (28 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Small ribosomal subunit protein us14 (6 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Small ribosomal subunit protein us11 (14 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Small ribosomal subunit protein es28 (7 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- 40s ribosomal protein s7 (21 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Eukaryotic translation initiation factor eif-1 (12 kDa, Protein from Saccharomyces cerevisiae S288C)
- Mrna (5'-r(p*ap*ap*u)-3') (15 kDa, RNA from Kluyveromyces lactis)
- Met-trnai (24 kDa, RNA from Saccharomyces cerevisiae S288C)
- Initiation factor eif1 (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l41-a (3 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- 40s ribosomal protein s26 (11 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Klla0b08173p (12 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- 40s ribosomal protein s12 (14 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Klla0e12277p (35 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Small ribosomal subunit protein es31 (17 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- 40s ribosomal protein s25 (12 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Structure of a yeast 48s-auc preinitiation complex in closed conformation (1 MDa, Complex)
- Klla0f18040p (16 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Small ribosomal subunit protein es1 (28 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- 18s ribosomal rna (579 kDa, RNA from Kluyveromyces lactis NRRL Y-1140)
- Klla0a07194p (15 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- 40s ribosomal protein s24 (15 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Klla0d10659p (25 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Small ribosomal subunit protein us5 (27 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Small ribosomal subunit protein us9 (15 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
Structure of a yeast 48S-AUC preinitiation complex in closed conformation (model py48S-AUC-3.2)
Resolution: 3.95 Å
EM Method: Single-particle
Fitted PDBs: 8s8f
Q-score: 0.352
Villamayor-Belinchon L, Sharma P, Gordiyenko Y, Llacer JL, Hussain T
Nucleic Acids Res (2024) 52 pp. 11317-11335 [ DOI: doi:10.1093/nar/gkae737 Pubmed: 39193907 ]
- Eukaryotic translation initiation factor 1a (17 kDa, Protein from Saccharomyces cerevisiae S288C)
- Eukaryotic translation initiation factor 2 subunit gamma (57 kDa, Protein from Saccharomyces cerevisiae S288C)
- 40s ribosomal protein s27 (8 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Klla0e23673p (21 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Mrna (Complex from Kluyveromyces lactis)
- Methionine (149 Da, Ligand)
- Small ribosomal subunit protein us10 (13 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Klla0a10483p (17 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Eukaryotic translation initiation factor 2 subunit alpha (34 kDa, Protein from Saccharomyces cerevisiae S288C)
- Small ribosomal subunit protein us8 (14 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Ribosome (Complex from Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37))
- 40s ribosomal protein s8 (22 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Initiation factor eif1a (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Eukaryotic translation initiation factor 2 subunit beta (31 kDa, Protein from Saccharomyces cerevisiae S288C)
- 40s ribosomal protein s26 (13 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- 40s ribosomal protein s3 (26 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Trna (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Klla0b01474p (15 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Klla0f07843p (15 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Klla0b01562p (17 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Small ribosomal subunit protein es6 (26 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Phosphomethylphosphonic acid guanylate ester (521 Da, Ligand)
- Water (18 Da, Ligand)
- 40s ribosomal protein s21 (9 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Magnesium ion (24 Da, Ligand)
- 40s ribosomal protein l41-a (3 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Zinc ion (65 Da, Ligand)
- Klla0b11231p (16 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- 40s ribosomal protein s4 (29 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Initiation factor eif2 (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s30 (7 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Small ribosomal subunit protein us2 (28 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Small ribosomal subunit protein us14 (6 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Small ribosomal subunit protein us11 (14 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Small ribosomal subunit protein es28 (7 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- 40s ribosomal protein s7 (21 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Mrna (5'-r(p*ap*ap*u)-3') (15 kDa, RNA from Kluyveromyces lactis)
- Met-trnai (24 kDa, RNA from Saccharomyces cerevisiae S288C)
- Eukaryotic translation initiation factor eif-1 (12 kDa, Protein from Saccharomyces cerevisiae S288C)
- Initiation factor eif1 (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Klla0e12277p (35 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Klla0b08173p (12 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- 40s ribosomal protein s12 (14 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Small ribosomal subunit protein es31 (17 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- 40s ribosomal protein s25 (12 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Structure of a yeast 48s-auc preinitiation complex in closed conformation (1 MDa, Complex)
- Klla0f18040p (16 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Small ribosomal subunit protein es1 (28 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- 18s ribosomal rna (579 kDa, RNA from Kluyveromyces lactis NRRL Y-1140)
- Klla0a07194p (15 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- 40s ribosomal protein s24 (15 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Klla0d10659p (25 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Small ribosomal subunit protein us5 (27 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Small ribosomal subunit protein us9 (15 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
Structure of a yeast 48S-AUC preinitiation complex in closed conformation (model py48S-AUC-eIF1)
Villamayor-Belinchon L, Sharma P, Gordiyenko Y, Llacer JL, Hussain T
Nucleic Acids Res (2024) 52 pp. 11317-11335 [ Pubmed: 39193907 DOI: doi:10.1093/nar/gkae737 ]
- 40s ribosomal protein s21 (9 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Phosphomethylphosphonic acid guanylate ester (521 Da, Ligand)
- 18s ribosomal rna (579 kDa, RNA from Kluyveromyces lactis NRRL Y-1140)
- Small ribosomal subunit protein us5 (27 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Small ribosomal subunit protein us8 (14 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Water (18 Da, Ligand)
- Klla0a07194p (15 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Zinc ion (65 Da, Ligand)
- Small ribosomal subunit protein es28 (7 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Eukaryotic translation initiation factor 2 subunit gamma (57 kDa, Protein from Saccharomyces cerevisiae S288C)
- Small ribosomal subunit protein us10 (13 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Methionine (149 Da, Ligand)
- 40s ribosomal protein s7 (21 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Magnesium ion (24 Da, Ligand)
- Small ribosomal subunit protein us14 (6 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Eukaryotic translation initiation factor eif-1 (12 kDa, Protein from Saccharomyces cerevisiae S288C)
- 40s ribosomal protein s30 (7 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Ribosome (Complex from Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37))
- Small ribosomal subunit protein es1 (28 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Klla0e12277p (35 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Small ribosomal subunit protein es6 (26 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Klla0d10659p (25 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Klla0b08173p (12 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Initiation factor eif2 (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s3 (26 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Mrna (5'-r(p*ap*ap*u)-3') (15 kDa, RNA from Kluyveromyces lactis)
- 40s ribosomal protein s4 (29 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Small ribosomal subunit protein us2 (28 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Structure of a yeast 48s-auc preinitiation complex in closed conformation (1 MDa, Complex)
- 40s ribosomal protein s24 (15 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Klla0a10483p (17 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- 40s ribosomal protein s25 (12 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Klla0f18040p (16 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Mrna (Complex from Kluyveromyces lactis)
- Small ribosomal subunit protein es31 (17 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Initiation factor eif1a (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Initiation factor eif1 (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Klla0b01562p (17 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Klla0e23673p (21 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Small ribosomal subunit protein us11 (14 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Klla0f07843p (15 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Eukaryotic translation initiation factor 2 subunit alpha (34 kDa, Protein from Saccharomyces cerevisiae S288C)
- Eukaryotic translation initiation factor 1a (17 kDa, Protein from Saccharomyces cerevisiae S288C)
- 40s ribosomal protein s8 (22 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- 40s ribosomal protein l41-a (3 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- 40s ribosomal protein s12 (14 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Met-trnai (24 kDa, RNA from Saccharomyces cerevisiae S288C)
- Trna (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Eukaryotic translation initiation factor 2 subunit beta (31 kDa, Protein from Saccharomyces cerevisiae S288C)
- Klla0b11231p (16 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Klla0b01474p (15 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Small ribosomal subunit protein us9 (15 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- 40s ribosomal protein s26 (13 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- 40s ribosomal protein s27 (8 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
Structure of a yeast ABCE1-bound 43S pre-initiation complex
Resolution: 5.3 Å
EM Method: Single-particle
Fitted PDBs: 6zce
Q-score: 0.231
Kratzat H, Mackens-Kiani T, Ameismeier M, Potocnjak M, Cheng J, Dacheux E, Namane A, Berninghausen O, Herzog F, Fromont-Racine M, Becker T, Beckmann R
EMBO J (2021) 40 pp. e105179-e105179 [ Pubmed: 33289941 DOI: doi:10.15252/embj.2020105179 ]
- 40s ribosomal protein s0-a (28 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s17-a (15 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s4-a (29 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s23-a (16 kDa, Protein from Baker's yeast)
- Zinc ion (65 Da, Ligand)
- Abce1-bound 43s initiation complex (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Eukaryotic translation initiation factor 3 subunit i (38 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s12 (15 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s3 (26 kDa, Protein from Baker's yeast)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Translation initiation factor rli1 (68 kDa, Protein from Baker's yeast)
- Magnesium ion (24 Da, Ligand)
- 40s ribosomal protein s5 (25 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s15 (15 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s7-a (21 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s6-a (27 kDa, Protein from Baker's yeast)
- Eukaryotic translation initiation factor 3 subunit j (29 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s2 (27 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s9-a (22 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s18-a (17 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s19-a (15 kDa, Protein from Baker's yeast)
- Eukaryotic translation initiation factor 3 subunit c (93 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s26-a (13 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s11-a (17 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s13 (17 kDa, Protein from Baker's yeast)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- 18s ribosomal rna (1719-mer) (579 kDa, RNA from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Iron/sulfur cluster (351 Da, Ligand)
- 40s ribosomal protein s22-a (14 kDa, Protein from Baker's yeast)
- Eukaryotic translation initiation factor 3 subunit g (30 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s8-a (22 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s14-a (14 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s1-a (28 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s16-a (15 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s20 (13 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s25-a (12 kDa, Protein from Baker's yeast)
- Rna recognition motif (unknown) (5 kDa, Protein from Saccharomyces cerevisiae S288C)
- 40s ribosomal protein s27-a (8 kDa, Protein from Baker's yeast)
- Eukaryotic translation initiation factor eif-1 (12 kDa, Protein from Baker's yeast)
- Guanine nucleotide-binding protein subunit beta-like protein (34 kDa, Protein from Baker's yeast)
- Eukaryotic translation initiation factor 3 subunit a (110 kDa, Protein from Baker's yeast)
- Ubiquitin-40s ribosomal protein s31 (17 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s24-a (15 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s28-b (7 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s29-a (6 kDa, Protein from Baker's yeast)
- Eukaryotic translation initiation factor 3 subunit b (88 kDa, Protein from Baker's yeast)
- Eukaryotic translation initiation factor 1a (17 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s30-a (7 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s10-a (12 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s21-a (9 kDa, Protein from Baker's yeast)
Cryo-EM structure of a partial yeast 48S preinitiation complex in its open conformation.
Resolution: 5.15 Å
EM Method: Single-particle
Fitted PDBs: 6gsm
Q-score: 0.197
Llacer JL, Hussain T, Dong J, Villamayor L, Gordiyenko Y, Hinnebusch AG
Nucleic Acids Res (2021) [ DOI: doi:10.1093/nar/gkab908 Pubmed: 34648019 ]
- Klla0b06182p (7 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Ubiquitin-40s ribosomal protein s27a (7 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- 40s ribosomal protein s4 (29 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Klla0e23673p (20 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Klla0f07843p (13 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Klla0f25542p (12 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Trna (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Eukaryotic translation initiation factor 2 subunit beta (16 kDa, Protein from Saccharomyces cerevisiae S288C)
- Mrna (Complex from Kluyveromyces lactis)
- 40s ribosomal protein s12 (12 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Eukaryotic translation initiation factor 3 subunit i (38 kDa, Protein from Saccharomyces cerevisiae S288C)
- 40s ribosomal protein s8 (22 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- 40s ribosomal protein s29 (6 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- 40s ribosomal protein s30 (6 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Klla0d08305p (24 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Klla0f18040p (16 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Eukaryotic translation initiation factor 2 subunit gamma (47 kDa, Protein from Saccharomyces cerevisiae S288C)
- Eukaryotic translation initiation factor eif-1 (10 kDa, Protein from Saccharomyces cerevisiae S288C)
- Ribosome (Complex from Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37))
- Zinc ion (65 Da, Ligand)
- 40s ribosomal protein s22 (14 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- 40s ribosomal protein s7 (21 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Klla0a10483p (17 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- 40s ribosomal protein s21 (9 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- 40s ribosomal protein s1 (26 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Initiation factors eif1 and eif1a (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Klla0a07194p (15 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- [(2~{r},3~{s},4~{r},5~{r})-5-(6-aminopurin-9-yl)-4-oxidanyl-2-(phosphonooxymethyl)oxolan-3-yl] (2~{s})-2-azanyl-4-methylsulfanyl-butanoate (478 Da, Ligand)
- Mrna (5'-r(p*ap*ap*u)-3') (919 Da, RNA from Kluyveromyces lactis)
- Eukaryotic translation initiation factor 3 subunit b (75 kDa, Protein from Saccharomyces cerevisiae S288C)
- 40s ribosomal protein s14 (13 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- 40s ribosomal protein s28 (6 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Met-trnai (24 kDa, RNA from Saccharomyces cerevisiae S288C)
- 40s ribosomal protein s24 (15 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- 60s ribosomal protein l41-a (3 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Structure of a partial yeast 48s preinitiation complex in open conformation (1 MDa, Complex)
- Eukaryotic translation initiation factor 3 subunit a,eukaryotic translation initiation factor 3 subunit a,eif3a (62 kDa, Protein from Saccharomyces cerevisiae S288C)
- Initiation factors eif2 and eif3 (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Klla0e12277p (35 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- 40s ribosomal protein s16 (15 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Klla0b01562p (16 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Eukaryotic translation initiation factor 3 subunit c (76 kDa, Protein from Saccharomyces cerevisiae S288C)
- Eukaryotic translation initiation factor 2 subunit alpha (30 kDa, Protein from Saccharomyces cerevisiae S288C)
- 40s ribosomal protein s26 (11 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- 40s ribosomal protein s0 (23 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Klla0f09812p (23 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Eukaryotic translation initiation factor 3 subunit g (5 kDa, Protein from Saccharomyces cerevisiae S288C)
- Klla0b08173p (11 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- 40s ribosomal protein s27 (8 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Klla0b11231p (15 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Phosphomethylphosphonic acid guanylate ester (521 Da, Ligand)
- 18s ribosomal rna (579 kDa, RNA from Kluyveromyces lactis NRRL Y-1140)
- Klla0d10659p (22 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Magnesium ion (24 Da, Ligand)
- Klla0b01474p (14 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- 40s ribosomal protein s6 (25 kDa, Protein from Kluyveromyces lactis NRRL Y-1140)
- Eukaryotic translation initiation factor 1a (10 kDa, Protein from Saccharomyces cerevisiae S288C)
Resolution: 5.75 Å
EM Method: Single-particle
Fitted PDBs: 6gsn
Q-score: 0.185
Llacer JL, Hussain T, Dong J, Villamayor L, Gordiyenko Y, Hinnebusch AG
Nucleic Acids Res (2021) [ DOI: doi:10.1093/nar/gkab908 Pubmed: 34648019 ]
- 40s ribosomal protein s29 (6 kDa, Protein from Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37))
- Klla0a10483p (17 kDa, Protein from Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37))
- Eukaryotic translation initiation factor 2 subunit gamma (47 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Klla0f25542p (12 kDa, Protein from Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37))
- 18s rrna (1798-mer) (579 kDa, RNA from Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37))
- Eukaryotic translation initiation factor 3 subunit i (38 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Magnesium ion (24 Da, Ligand)
- Klla0a07194p (15 kDa, Protein from Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37))
- Klla0e23673p (20 kDa, Protein from Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37))
- Eukaryotic translation initiation factor 1a (12 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s24 (15 kDa, Protein from Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37))
- Klla0d10659p (22 kDa, Protein from Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37))
- Trna (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Klla0b08173p (11 kDa, Protein from Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37))
- Zinc ion (65 Da, Ligand)
- 40s ribosomal protein s28 (6 kDa, Protein from Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37))
- Trnai (75-mer) (24 kDa, RNA from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s22 (14 kDa, Protein from Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37))
- 40s ribosomal protein s26 (11 kDa, Protein from Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37))
- 40s ribosomal protein s4 (29 kDa, Protein from Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37))
- Klla0b01474p (14 kDa, Protein from Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37))
- Ribosome (Cellular component from Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37))
- Eukaryotic translation initiation factor 3 subunit a,eif3a (62 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s21 (9 kDa, Protein from Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37))
- Methionine (149 Da, Ligand)
- 40s ribosomal protein s12 (12 kDa, Protein from Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37))
- Mrna (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l41-a (3 kDa, Protein from Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37))
- Mrna (4 kDa, RNA from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Eukaryotic translation initiation factor 3 subunit g (5 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s30 (6 kDa, Protein from Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37))
- Structure of a partial yeast 48s preinitiation complex in closed conformation (1 MDa, Complex)
- Ubiquitin-40s ribosomal protein s27a (7 kDa, Protein from Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37))
- Eukaryotic translation initiation factor 2 subunit alpha (30 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Eukaryotic translation initiation factor 3 subunit c (80 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Eukaryotic translation initiation factor 2 subunit beta (16 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s8 (22 kDa, Protein from Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37))
- 40s ribosomal protein s7 (21 kDa, Protein from Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37))
- Klla0d08305p (24 kDa, Protein from Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37))
- 40s ribosomal protein s6 (25 kDa, Protein from Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37))
- Rps23 (15 kDa, Protein from Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37))
- Initiation factors (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Klla0e12277p (35 kDa, Protein from Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37))
- 40s ribosomal protein s27 (8 kDa, Protein from Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37))
- Klla0f18040p (16 kDa, Protein from Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37))
- Klla0f09812p (23 kDa, Protein from Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37))
- Klla0b01562p (16 kDa, Protein from Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37))
- 40s ribosomal protein s1 (26 kDa, Protein from Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37))
- 40s ribosomal protein s14 (13 kDa, Protein from Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37))
- Eukaryotic translation initiation factor eif-1 (10 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s16 (15 kDa, Protein from Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37))
- Klla0b06182p (7 kDa, Protein from Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37))
- Phosphomethylphosphonic acid guanylate ester (521 Da, Ligand)
- Eukaryotic translation initiation factor 3 subunit b (76 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Klla0f07843p (13 kDa, Protein from Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37))
- 40s ribosomal protein s0 (24 kDa, Protein from Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37))