Structure of rat beta-arrestin 1 by fiducial-assisted cryo-EM
Resolution: 3.52 Å
EM Method: Single-particle
Fitted PDBs: 9dng
Q-score: 0.431
Kahsai AW, Pakharukova N, Kwon HY, Shah KS, Liang-Lin JG, Del Real CT, Shim PJ, Lee MA, Ngo VA, Shreiber BN, Liu S, Schwalb AM, Espinoza EF, Thomas BN, Kunzle CA, Smith JS, Wang J, Kim J, Zhang X, Rockman HA, Thomsen ARB, Rein LAM, Shi L, Ahn S, Masoudi A, Lefkowitz RJ
bioRxiv (2024) [ Pubmed: 39763753 DOI: doi:10.1101/2024.12.27.630464 ]
- Beta-arrestin 1 with insertion of soluble cytochrome b562 bound to anti-bril fab (Complex from Rattus norvegicus)
- Anti-bril fab heavy chain (23 kDa, Protein from Homo sapiens)
- Anti-bril fab light chain (23 kDa, Protein from Homo sapiens)
- Beta-arrestin-1,soluble cytochrome b562 (55 kDa, Protein from Rattus norvegicus)
Structure of rat beta-arrestin 1 bound to allosteric inhibitor
Resolution: 3.47 Å
EM Method: Single-particle
Fitted PDBs: 9dnm
Q-score: 0.466
Kahsai AW, Pakharukova N, Kwon HY, Shah KS, Liang-Lin JG, Del Real CT, Shim PJ, Lee MA, Ngo VA, Shreiber BN, Liu S, Schwalb AM, Espinoza EF, Thomas BN, Kunzle CA, Smith JS, Wang J, Kim J, Zhang X, Rockman HA, Thomsen ARB, Rein LAM, Shi L, Ahn S, Masoudi A, Lefkowitz RJ
bioRxiv (2024) [ Pubmed: 39763753 DOI: doi:10.1101/2024.12.27.630464 ]
- Beta-arrestin 1 with insertion of soluble cytochrome b562 bound to anti-bril fab (Complex from Rattus norvegicus)
- Anti-bril fab light chain (23 kDa, Protein from Homo sapiens)
- (1beta,6beta,7beta,8alpha,9beta,10alpha,13alpha,14r,16beta)-1,6,7,14-tetrahydroxy-7,20-epoxykauran-15-one (366 Da, Ligand)
- Beta-arrestin-1,soluble cytochrome b562 (55 kDa, Protein from Rattus norvegicus)
- Anti-bril fab heavy chain (23 kDa, Protein from Homo sapiens)
SFTSV Gn in complex with JK-8/12 Fab
Resolution: 2.37 Å
EM Method: Single-particle
Fitted PDBs: 8zhq
Q-score: 0.505
Zhang S, Shang H, Han S, Li J, Peng X, Wu Y, Yang X, Leng Y, Wang F, Cui N, Xu L, Zhang H, Guo Y, Xu X, Zhang N, Liu W, Li H
Ebiomedicine (2024) 111 pp. 105481-105481 [ Pubmed: 39644769 DOI: doi:10.1016/j.ebiom.2024.105481 ]
- Envelopment polyprotein (38 kDa, Protein from Severe fever with thrombocytopenia syndrome virus)
- Jk-8 fab heavy chain (23 kDa, Protein from Homo sapiens)
- Jk-8 fab light chain (23 kDa, Protein from Homo sapiens)
- Jk-12 fab light chain (22 kDa, Protein from Homo sapiens)
- Jk-12 fab heavy chain (22 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Ternary complex of sftsv gn with neutralizing antibodies fab jk-8 and jk-12 (150 kDa, Complex from Homo sapiens)
Cryo-EM structure of GPR55-Fab-Nb-ONO-9710531 complex
Resolution: 3.04 Å
EM Method: Single-particle
Fitted PDBs: 9iya
Q-score: 0.415
Chang H, Li X, Shen L, Ge X, Hao S, Wu L, Liu S, Liu J, Cherezov V, Hua T
Cell Res (2025) 35 pp. 80-83 [ Pubmed: 39482405 DOI: doi:10.1038/s41422-024-01046-8 ]
- Anti-fab nanobody (13 kDa, Protein from Homo sapiens)
- 4-(3-bromanyl-5-ethoxy-4-oxidanyl-phenyl)-~{n}-(2-methoxyphenyl)-2-methyl-5-oxidanylidene-4,6,7,8-tetrahydro-1~{h}-quinoline-3-carboxamide (527 Da, Ligand)
- Ternary complex of gpr55 with anti-bril fab and anti-fab nanobody (173 kDa, Complex from Homo sapiens)
- G-protein coupled receptor 55,soluble cytochrome b562 (47 kDa, Protein from Homo sapiens)
- Anti-bril fab light chain (23 kDa, Protein from Homo sapiens)
- Anti-bril fab heavy chain (26 kDa, Protein from Homo sapiens)
Cryo-EM structure of SNAP-94847-bound MCHR1, S1 state
Resolution: 3.33 Å
EM Method: Single-particle
Fitted PDBs: 8yns
Q-score: 0.476
Ye X, Liu G, Li X, He B, Tao Y, Guan J, Mu Y, Liu H, Gong W
Cell Discov (2024) 10 pp. 124-124 [ DOI: doi:10.1038/s41421-024-00754-0 Pubmed: 39616153 ]
- Antibody 1b3 fab light chain (23 kDa, Protein from synthetic construct)
- Snap-94847-mchr1-fab1b3-glue complex (Complex from Homo sapiens)
- Melanin-concentrating hormone receptor 1,soluble cytochrome b562 (64 kDa, Protein from Homo sapiens)
- Glue molecule 4-9 (30 kDa, Protein from synthetic construct)
- Antibody 1b3 fab heavy chain (25 kDa, Protein from synthetic construct)
- ~{n}-[3-[1-[[4-[3,4-bis(fluoranyl)phenoxy]phenyl]methyl]piperidin-4-yl]-4-methyl-phenyl]-2-methyl-propanamide (478 Da, Ligand)
Cryo-EM structure of SNAP-94847-bound MCHR1, S2 state
Resolution: 3.43 Å
EM Method: Single-particle
Fitted PDBs: 8ynt
Q-score: 0.422
Ye X, Liu G, Li X, He B, Tao Y, Guan J, Mu Y, Liu H, Gong W
Cell Discov (2024) 10 pp. 124-124 [ DOI: doi:10.1038/s41421-024-00754-0 Pubmed: 39616153 ]
- Antibody 1b3 fab light chain (23 kDa, Protein from synthetic construct)
- Snap-94847-mchr1-fab1b3-glue complex (Complex from Homo sapiens)
- Melanin-concentrating hormone receptor 1,soluble cytochrome b562 (64 kDa, Protein from Homo sapiens)
- Glue molecule 4-9 (30 kDa, Protein from synthetic construct)
- Antibody 1b3 fab heavy chain (25 kDa, Protein from synthetic construct)
- ~{n}-[3-[1-[[4-[3,4-bis(fluoranyl)phenoxy]phenyl]methyl]piperidin-4-yl]-4-methyl-phenyl]-2-methyl-propanamide (478 Da, Ligand)
Resolution: 2.6 Å
EM Method: Single-particle
Fitted PDBs: 9c44
Q-score: 0.535
Liu P, Huang ML, Guo H, McCallum M, Si JY, Chen YM, Wang CL, Yu X, Shi LL, Xiong Q, Ma CB, Bowen JE, Tong F, Liu C, Sun YH, Yang X, Chen J, Guo M, Li J, Corti D, Veesler D, Shi ZL, Yan H
Nature (2024) 635 pp. 978-986 [ DOI: doi:10.1038/s41586-024-08121-5 Pubmed: 39478224 ]
- Spike glycoprotein (142 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- S2l20 fab light chain variable region (11 kDa, Protein from Homo sapiens)
- Sars-cov-2 s bound by s2l20 fab (Complex from Homo sapiens)
- S2l20 fab heavy chain variable (13 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
SARS-CoV-2 S + S2L20 (local refinement of NTD and S2L20 Fab variable region)
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 9c45
Q-score: 0.552
Liu P, Huang ML, Guo H, McCallum M, Si JY, Chen YM, Wang CL, Yu X, Shi LL, Xiong Q, Ma CB, Bowen JE, Tong F, Liu C, Sun YH, Yang X, Chen J, Guo M, Li J, Corti D, Veesler D, Shi ZL, Yan H
Nature (2024) 635 pp. 978-986 [ DOI: doi:10.1038/s41586-024-08121-5 Pubmed: 39478224 ]
- Spike glycoprotein (142 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- S2l20 fab light chain variable region (11 kDa, Protein from Homo sapiens)
- Sars-cov-2 s bound by s2l20 fab (Complex from Homo sapiens)
- S2l20 fab heavy chain variable (13 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
Engineered peptide-specific binder in complex with HLA-DR1/CLIP
Resolution: 2.28 Å
EM Method: Single-particle
Fitted PDBs: 8vsj
Q-score: 0.528
Du H, Liu J, Jude KM, Yang X, Li Y, Bell B, Yang H, Kassardjian A, Blackson W, Mobedi A, Parekh U, Parra Sperberg RA, Julien JP, Mellins ED, Garcia KC, Huang PS
Nat Biotechnol (2024) [ Pubmed: 39672953 DOI: doi:10.1038/s41587-024-02466-y ]
- Hla class ii histocompatibility antigen, dr alpha chain (21 kDa, Protein from Homo sapiens)
- Engineered peptide-specific binder in complex with hla-dr1/clip (Complex from Homo sapiens)
- Hla class ii histocompatibility antigen dr beta chain (22 kDa, Protein from Homo sapiens)
- C44h10 fab light chain (23 kDa, Protein from Homo sapiens)
- C44h10 fab heavy chain (23 kDa, Protein from Homo sapiens)
- Superantigen (15 kDa, Protein from Metamycoplasma arthritidis)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Class-ii-associated invariant chain peptide (1 kDa, Protein from Homo sapiens)
Cryo-EM structure of ETAR bound with Macitentan
Hou J, Liu S, Zhang X, Tu G, Wu L, Zhang Y, Yang H, Li X, Liu J, Jiang L, Tan Q, Bai F, Liu Z, Miao C, Hua T, Luo Z
Cell Discov (2024) 10 pp. 79-79 [ DOI: doi:10.1038/s41421-024-00705-9 Pubmed: 39075075 ]
- Macitentan (588 Da, Ligand)
- Endoglucanase h,endothelin-1 receptor,soluble cytochrome b562 (89 kDa, Protein from Homo sapiens)
- Anti-bril fab light chain (25 kDa, Protein from Homo sapiens)
- Anti-fab nanobody (15 kDa, Protein from synthetic construct)
- Anti-bril fab heavy chain (28 kDa, Protein from Homo sapiens)
- Complex of endothelin receptor type a with macitentan (Complex from Homo sapiens)