Structure of the FDX2-bound core ISC complex (proximal conformation)
Resolution: 2.26 Å
EM Method: Single-particle
Fitted PDBs: 8rmc
Q-score: 0.702
Steinhilper R, Boss L, Freibert SA, Schulz V, Krapoth N, Kaltwasser S, Lill R, Murphy BJ
Nat Commun (2024) 15 pp. 10559-10559 [ DOI: doi:10.1038/s41467-024-54585-4 Pubmed: 39632806 ]
- Ferredoxin-2, mitochondrial (13 kDa, Protein from Homo sapiens)
- Isoform 1 of iron-sulfur cluster assembly enzyme iscu (15 kDa, Protein from Homo sapiens)
- S-[2-({n-[(2r)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate (540 Da, Ligand)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Fe (ii) ion (55 Da, Ligand)
- Isoform mitochondrial of cysteine desulfurase (45 kDa, Protein from Homo sapiens)
- Human fdx2-bound core isc complex (nfs1-isd11-acp1-iscu2-fdx2) (Complex from Homo sapiens)
- Lyr motif-containing protein 4 (13 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Acyl carrier protein (8 kDa, Protein from Escherichia coli BL21(DE3))
Structure of the FDX2-bound core ISC complex (distal conformation)
Resolution: 2.52 Å
EM Method: Single-particle
Fitted PDBs: 8rmd
Q-score: 0.642
Steinhilper R, Boss L, Freibert SA, Schulz V, Krapoth N, Kaltwasser S, Lill R, Murphy BJ
Nat Commun (2024) 15 pp. 10559-10559 [ DOI: doi:10.1038/s41467-024-54585-4 Pubmed: 39632806 ]
- Ferredoxin-2, mitochondrial (13 kDa, Protein from Homo sapiens)
- Isoform 1 of iron-sulfur cluster assembly enzyme iscu (15 kDa, Protein from Homo sapiens)
- S-[2-({n-[(2r)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate (540 Da, Ligand)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Fe (ii) ion (55 Da, Ligand)
- Isoform mitochondrial of cysteine desulfurase (45 kDa, Protein from Homo sapiens)
- Human fdx2-bound core isc complex (nfs1-isd11-acp1-iscu2-fdx2) (Complex from Homo sapiens)
- Lyr motif-containing protein 4 (13 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Acyl carrier protein (8 kDa, Protein from Escherichia coli BL21(DE3))
Structure of the core ISC complex under turnover conditions (FDX2-bound in proximal conformation)
Resolution: 2.33 Å
EM Method: Single-particle
Fitted PDBs: 8rmf
Q-score: 0.633
Steinhilper R, Boss L, Freibert SA, Schulz V, Krapoth N, Kaltwasser S, Lill R, Murphy BJ
Nat Commun (2024) 15 pp. 10559-10559 [ DOI: doi:10.1038/s41467-024-54585-4 Pubmed: 39632806 ]
- Ferredoxin-2, mitochondrial (13 kDa, Protein from Homo sapiens)
- Isoform 1 of iron-sulfur cluster assembly enzyme iscu (15 kDa, Protein from Homo sapiens)
- Human core isc complex (nfs1-isd11-acp1-iscu2-fxn/fdx2) under turnover conditions (Complex from Homo sapiens)
- S-[2-({n-[(2r)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate (540 Da, Ligand)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Fe (ii) ion (55 Da, Ligand)
- Isoform mitochondrial of cysteine desulfurase (45 kDa, Protein from Homo sapiens)
- Lyr motif-containing protein 4 (13 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Acyl carrier protein (8 kDa, Protein from Escherichia coli BL21(DE3))
Structure of the core ISC complex under turnover conditions (FDX2-bound in distal conformation)
Resolution: 2.46 Å
EM Method: Single-particle
Fitted PDBs: 8rmg
Q-score: 0.598
Steinhilper R, Boss L, Freibert SA, Schulz V, Krapoth N, Kaltwasser S, Lill R, Murphy BJ
Nat Commun (2024) 15 pp. 10559-10559 [ DOI: doi:10.1038/s41467-024-54585-4 Pubmed: 39632806 ]
- Ferredoxin-2, mitochondrial (13 kDa, Protein from Homo sapiens)
- Isoform 1 of iron-sulfur cluster assembly enzyme iscu (15 kDa, Protein from Homo sapiens)
- Human core isc complex (nfs1-isd11-acp1-iscu2-fxn/fdx2) under turnover conditions (Complex from Homo sapiens)
- S-[2-({n-[(2r)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate (540 Da, Ligand)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Fe (ii) ion (55 Da, Ligand)
- Isoform mitochondrial of cysteine desulfurase (45 kDa, Protein from Homo sapiens)
- Lyr motif-containing protein 4 (13 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Acyl carrier protein (8 kDa, Protein from Escherichia coli BL21(DE3))