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was set up in 1974 as Europe’s flagship laboratory for the life sciences – an intergovernmental organisation with more than 80 independent research groups covering the spectrum of molecular biology:

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Tissue biology and disease modelling

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EMBL-EBI: European Bioinformatics Institute

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sample_name:"Flagellar radial spoke protein 6"

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EMD-40220

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96-nm repeat unit of doublet microtubules from Chlamydomonas reinhardtii flagella

Release date: May 31, 2023
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 8glv

Walton T, Gui M, Velkova S, Fassad MR, Hirst RA, Haarman E, O'Callaghan C, Bottier M, Burgoyne T, Mitchison HM, Brown A

Nature (2023) 618 pp. 625-633 [ DOI: doi:10.1038/s41586-023-06140-2 Pubmed: 37258679 ]

Sample:

Outer dynein arm protein 1 (62 kDa, Protein from Chlamydomonas reinhardtii)
Dynein 11 kda light chain, flagellar outer arm (13 kDa, Protein from Chlamydomonas reinhardtii)
Fap143 (29 kDa, Protein from Chlamydomonas reinhardtii)
Duf4201 domain-containing protein (45 kDa, Protein from Chlamydomonas reinhardtii)
Flagellar associated protein (53 kDa, Protein from Chlamydomonas reinhardtii)
Fap251 (97 kDa, Protein from Chlamydomonas reinhardtii)
Axonemal inner arm i1 intermediate chain dynein ic138 (111 kDa, Protein from Chlamydomonas reinhardtii)
Cilia- and flagella-associated protein 100 (65 kDa, Protein from Chlamydomonas reinhardtii)
Flagellar outer dynein arm heavy chain beta (520 kDa, Protein from Chlamydomonas reinhardtii)
Protein flattop homolog (15 kDa, Protein from Chlamydomonas reinhardtii)
Subunit of axonemal inner dynein (40 kDa, Protein from Chlamydomonas reinhardtii)
Fap21 (49 kDa, Protein from Chlamydomonas reinhardtii)
Fap339 (10 kDa, Protein from Chlamydomonas reinhardtii)
Flagellar radial spoke protein 5 (55 kDa, Protein from Chlamydomonas reinhardtii)
Unknown (2 kDa, Protein from Chlamydomonas reinhardtii)
Aaa+ atpase domain-containing protein (470 kDa, Protein from Chlamydomonas reinhardtii)
Tubulin beta (49 kDa, Protein from Chlamydomonas reinhardtii)
Drc5 (43 kDa, Protein from Chlamydomonas reinhardtii)
Dynein regulatory complex protein 1 (79 kDa, Protein from Chlamydomonas reinhardtii)
Flagellar associated protein (43 kDa, Protein from Chlamydomonas reinhardtii)
Flagellar associated protein (48 kDa, Protein from Chlamydomonas reinhardtii)
Unknown (6 kDa, Protein from Chlamydomonas reinhardtii)
Fap43 (199 kDa, Protein from Chlamydomonas reinhardtii)
96-nm repeat unit of doublet microtubules from the flagella of chlamydomonas reinhardtii (Complex from Chlamydomonas reinhardtii)
Actin (41 kDa, Protein from Chlamydomonas reinhardtii)
Subunit of axonemal inner dynein (46 kDa, Protein from Chlamydomonas reinhardtii)
Cilia- and flagella-associated protein 299 (27 kDa, Protein from Chlamydomonas reinhardtii)
Radial spoke protein 9 (29 kDa, Protein from Chlamydomonas reinhardtii)
Meiosis-specific nuclear structural protein 1 (59 kDa, Protein from Chlamydomonas reinhardtii)
Dynein regulatory complex protein 8 (18 kDa, Protein from Chlamydomonas reinhardtii)
Cilia- and flagella-associated protein 58 (102 kDa, Protein from Chlamydomonas reinhardtii)
Dynein gamma chain, flagellar outer arm (513 kDa, Protein from Chlamydomonas reinhardtii)
Dynein regulatory complex protein 10 (40 kDa, Protein from Chlamydomonas reinhardtii)
Flagellar associated protein (35 kDa, Protein from Chlamydomonas reinhardtii)
Guanosine-5'-diphosphate (443 Da, Ligand)
Dynein regulatory complex subunit 4 (55 kDa, Protein from Chlamydomonas reinhardtii)
Cilia- and flagella-associated protein 77 (29 kDa, Protein from Chlamydomonas reinhardtii)
Wd_repeats_region domain-containing protein (146 kDa, Protein from Chlamydomonas reinhardtii)
Flagellar radial spoke protein 2 (77 kDa, Protein from Chlamydomonas reinhardtii)
Radial spoke protein 15 (35 kDa, Protein from Chlamydomonas reinhardtii)
Dynein regulatory complex subunit 2 (64 kDa, Protein from Chlamydomonas reinhardtii)
Axonemal inner arm dynein i1/f subunit (31 kDa, Protein from Chlamydomonas reinhardtii)
Fap385 (8 kDa, Protein from Chlamydomonas reinhardtii)
Radial spike protein 8 (40 kDa, Protein from Chlamydomonas reinhardtii)
Nucleoside diphosphate kinase 6 (61 kDa, Protein from Chlamydomonas reinhardtii)
Flagellar associated protein (39 kDa, Protein from Chlamydomonas reinhardtii)
Fap306 (20 kDa, Protein from Chlamydomonas reinhardtii)
Dhc_n2 domain-containing protein (465 kDa, Protein from Chlamydomonas reinhardtii)
Radial spoke protein 16 (39 kDa, Protein from Chlamydomonas reinhardtii)
Dynein, 78 kda intermediate chain, flagellar outer arm (76 kDa, Protein from Chlamydomonas reinhardtii)
Tubulin alpha (49 kDa, Protein from Chlamydomonas reinhardtii)
Outer dynein arm-docking complex subunit 1 (83 kDa, Protein from Chlamydomonas reinhardtii)
Flagellar associated protein (15 kDa, Protein from Chlamydomonas reinhardtii)
Flagellar associated protein (22 kDa, Protein from Chlamydomonas reinhardtii)
Flagellar associated protein (25 kDa, Protein from Chlamydomonas reinhardtii)
Ic97/casc1 n-terminal domain-containing protein (81 kDa, Protein from Chlamydomonas reinhardtii)
Peptidyl-prolyl cis-trans isomerase (19 kDa, Protein from Chlamydomonas reinhardtii)
Dynein 8 kda light chain, flagellar outer arm (10 kDa, Protein from Chlamydomonas reinhardtii)
Flagellar associated protein (267 kDa, Protein from Chlamydomonas reinhardtii)
Radial spoke protein 10 (23 kDa, Protein from Chlamydomonas reinhardtii)
Guanosine-5'-triphosphate (523 Da, Ligand)
Dynein regulatory complex subunit 6 (28 kDa, Protein from Chlamydomonas reinhardtii)
Cilia- and flagella-associated protein 61 (121 kDa, Protein from Chlamydomonas reinhardtii)
Flagellar associated protein (32 kDa, Protein from Chlamydomonas reinhardtii)
Dynein-1-beta heavy chain, flagellar inner arm i1 complex (511 kDa, Protein from Chlamydomonas reinhardtii)
Flagellar-associated protein 172 (101 kDa, Protein from Chlamydomonas reinhardtii)
Flagellar outer dynein arm light chain 2 (15 kDa, Protein from Chlamydomonas reinhardtii)
Inner-arm dynein f/i1 light chain (26 kDa, Protein from Chlamydomonas reinhardtii)
Aaa+ atpase domain-containing protein (478 kDa, Protein from Chlamydomonas reinhardtii)
Radial spoke protein 3 (56 kDa, Protein from Chlamydomonas reinhardtii)
Cfap91 domain-containing protein (134 kDa, Protein from Chlamydomonas reinhardtii)
Dynein light chain roadblock lc7b (11 kDa, Protein from Chlamydomonas reinhardtii)
Flagellar associated protein (25 kDa, Protein from Chlamydomonas reinhardtii)
Coiled-coil protein associated with protofilament ribbons (42 kDa, Protein from Chlamydomonas reinhardtii)
Cilia- and flagella-associated protein 73 (35 kDa, Protein from Chlamydomonas reinhardtii)
Flagellar associated protein (26 kDa, Protein from Chlamydomonas reinhardtii)
Fap1 (21 kDa, Protein from Chlamydomonas reinhardtii)
Duf4201 domain-containing protein (55 kDa, Protein from Chlamydomonas reinhardtii)
Atpase aaa-type core domain-containing protein (94 kDa, Protein from Chlamydomonas reinhardtii)
Cilia- and flagella-associated protein 45 (59 kDa, Protein from Chlamydomonas reinhardtii)
Flagellar-associated protein 59 (105 kDa, Protein from Chlamydomonas reinhardtii)
Fap52 (68 kDa, Protein from Chlamydomonas reinhardtii)
Flagellar associated protein (42 kDa, Protein from Chlamydomonas reinhardtii)
Cytochrome b5 heme-binding domain-containing protein (26 kDa, Protein from Chlamydomonas reinhardtii)
Calmodulin (18 kDa, Protein from Chlamydomonas reinhardtii)
Flagellar associated protein (15 kDa, Protein from Chlamydomonas reinhardtii)
Dynein axonemal light chain 1 (22 kDa, Protein from Chlamydomonas reinhardtii)
Dynein 14 kda light chain, flagellar outer arm (14 kDa, Protein from Chlamydomonas reinhardtii)
Radial spoke protein 4 (49 kDa, Protein from Chlamydomonas reinhardtii)
Fap34 (29 kDa, Protein from Chlamydomonas reinhardtii)
Radial spoke protein 14 (40 kDa, Protein from Chlamydomonas reinhardtii)
Fap129 (51 kDa, Protein from Chlamydomonas reinhardtii)
Dynein regulatory complex protein 9 (46 kDa, Protein from Chlamydomonas reinhardtii)
Fap253 (75 kDa, Protein from Chlamydomonas reinhardtii)
Dynein regulatory complex subunit 3 (60 kDa, Protein from Chlamydomonas reinhardtii)
Pacrg (34 kDa, Protein from Chlamydomonas reinhardtii)
Radial spoke protein 7 (54 kDa, Protein from Chlamydomonas reinhardtii)
Fap166 (23 kDa, Protein from Chlamydomonas reinhardtii)
Caltractin (19 kDa, Protein from Chlamydomonas reinhardtii)
Flagellar radial spoke protein 1 (87 kDa, Protein from Chlamydomonas reinhardtii)
Outer dynein arm-docking complex protein dc3 (21 kDa, Protein from Chlamydomonas reinhardtii)
Heavy chain alpha (504 kDa, Protein from Chlamydomonas reinhardtii)
Flagellar associated protein (12 kDa, Protein from Chlamydomonas reinhardtii)
Wd_repeats_region domain-containing protein (63 kDa, Protein from Chlamydomonas reinhardtii)
Dynein light chain 10 (12 kDa, Protein from Chlamydomonas reinhardtii)
Cilia- and flagella-associated protein 53 (56 kDa, Protein from Chlamydomonas reinhardtii)
Flagellar associated protein (16 kDa, Protein from Chlamydomonas reinhardtii)
Fap210 (53 kDa, Protein from Chlamydomonas reinhardtii)
Dynein light chain tctex1 (12 kDa, Protein from Chlamydomonas reinhardtii)
Fap207 (28 kDa, Protein from Chlamydomonas reinhardtii)
Fap44 (222 kDa, Protein from Chlamydomonas reinhardtii)
Aaa+ atpase domain-containing protein (468 kDa, Protein from Chlamydomonas reinhardtii)
Ic140 (109 kDa, Protein from Chlamydomonas reinhardtii)
Flagellar radial spoke protein 6 (48 kDa, Protein from Chlamydomonas reinhardtii)
Flagellar associated protein (177 kDa, Protein from Chlamydomonas reinhardtii)
Inner arm dynein light chain (13 kDa, Protein from Chlamydomonas reinhardtii)
Radial spoke protein 11 (21 kDa, Protein from Chlamydomonas reinhardtii)
Dynein 18 kda light chain, flagellar outer arm (17 kDa, Protein from Chlamydomonas reinhardtii)
Dhc2 (467 kDa, Protein from Chlamydomonas reinhardtii)
Nucleoside diphosphate kinase (41 kDa, Protein from Chlamydomonas reinhardtii)
Dynein light chain roadblock lc7a (11 kDa, Protein from Chlamydomonas reinhardtii)
Dynein 16 kda light chain, flagellar outer arm (17 kDa, Protein from Chlamydomonas reinhardtii)
Aaa+ atpase domain-containing protein (523 kDa, Protein from Chlamydomonas reinhardtii)
Cilia- and flagella-associated protein 20 (22 kDa, Protein from Chlamydomonas reinhardtii)
Magnesium ion (24 Da, Ligand)
28 kda inner dynein arm light chain, axonemal (28 kDa, Protein from Chlamydomonas reinhardtii)
Dynein light chain 9 (12 kDa, Protein from Chlamydomonas reinhardtii)
Flagellar associated protein (27 kDa, Protein from Chlamydomonas reinhardtii)
Protofilament ribbon protein of flagellar microtubules (72 kDa, Protein from Chlamydomonas reinhardtii)
Aaa+ atpase domain-containing protein (464 kDa, Protein from Chlamydomonas reinhardtii)
Flagellar associated protein (27 kDa, Protein from Chlamydomonas reinhardtii)

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EMD-22444

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Chlamydomonas reinhardtii radial spoke minimal head complex

Release date: Dec. 16, 2020
Resolution: 2.95 Å
EM Method: Single-particle
Fitted PDBs: 7jr9
Q-score: 0.575

Grossman-Haham I, Coudray N, Yu Z, Wang F, Zhang N, Bhabha G, Vale RD

Nat Struct Mol Biol (2021) 28 pp. 20-28 [ Pubmed: 33318704 DOI: doi:10.1038/s41594-020-00519-9 ]

Sample:

Radial spoke minimal head complex (Complex from Chlamydomonas reinhardtii)
Flagellar radial spoke protein 6 (48 kDa, Protein from Chlamydomonas reinhardtii)
Flagellar radial spoke protein 10 (528 Da, Protein from Chlamydomonas reinhardtii)
Radial spoke protein 10 (23 kDa, Protein from Chlamydomonas reinhardtii)
Flagellar radial spoke protein 6 (698 Da, Protein from Chlamydomonas reinhardtii)
Flagellar radial spoke protein 4 (52 kDa, Protein from Chlamydomonas reinhardtii)
Radial spoke protein 9 (29 kDa, Protein from Chlamydomonas reinhardtii)

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EMD-22446

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Chlamydomonas reinhardtii radial spoke head and neck (recombinant)

Release date: Dec. 16, 2020
Resolution: 3.03 Å
EM Method: Single-particle
Fitted PDBs: 7jrj
Q-score: 0.527

Grossman-Haham I, Coudray N, Yu Z, Wang F, Zhang N, Bhabha G, Vale RD

Nat Struct Mol Biol (2021) 28 pp. 20-28 [ Pubmed: 33318704 DOI: doi:10.1038/s41594-020-00519-9 ]

Sample:

Radial spoke protein 9 (29 kDa, Protein from Chlamydomonas reinhardtii)
Flagellar radial spoke protein 2 (5 kDa, Protein from Chlamydomonas reinhardtii)
Flagellar radial spoke protein 1 (7 kDa, Protein from Chlamydomonas reinhardtii)
Flagellar radial spoke protein 3 (39 kDa, Protein from Chlamydomonas reinhardtii)
Flagellar radial spoke protein 6 (528 Da, Protein from Chlamydomonas reinhardtii)
Nucleoside diphosphate kinase 6 (61 kDa, Protein from Chlamydomonas reinhardtii)
Radial spoke protein 10 (23 kDa, Protein from Chlamydomonas reinhardtii)
Flagellar radial spoke protein 1 (87 kDa, Protein from Chlamydomonas reinhardtii)
Flagellar radial spoke protein 4 (49 kDa, Protein from Chlamydomonas reinhardtii)
Flagellar radial spoke protein 6 (48 kDa, Protein from Chlamydomonas reinhardtii)
Flagellar radial spoke protein 10 (443 Da, Protein from Chlamydomonas reinhardtii)
Chlamydomonas reinhardtii radial spoke head and neck (Complex from Chlamydomonas reinhardtii)
Unknown protein (358 Da, Protein from Chlamydomonas reinhardtii)
Flagellar radial spoke protein 5 (55 kDa, Protein from Chlamydomonas reinhardtii)
Flagellar radial spoke protein 2 (77 kDa, Protein from Chlamydomonas reinhardtii)

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EMD-22475

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Composite cryo-EM density map of the radial spoke 1 isolated from Chlamydomonas reinhardtii

Release date: Dec. 16, 2020
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7jtk
Q-score: 0.501

Gui M, Ma M, Sze-Tu E, Wang X, Koh F, Zhong ED, Berger B, Davis JH, Dutcher SK, Zhang R, Brown A

Nat Struct Mol Biol (2021) 28 pp. 29-37 [ DOI: doi:10.1038/s41594-020-00530-0 Pubmed: 33318703 ]

Sample:

Uncharacterized protein (75 kDa, Protein from Chlamydomonas reinhardtii)
Flagellar radial spoke protein 5 (55 kDa, Protein from Chlamydomonas reinhardtii)
Dynein 8 kda light chain, flagellar outer arm (10 kDa, Protein from Chlamydomonas reinhardtii)
Flagellar radial spoke protein 4 (49 kDa, Protein from Chlamydomonas reinhardtii)
Flagellar radial spoke protein 2 (77 kDa, Protein from Chlamydomonas reinhardtii)
Flagellar radial spoke protein 12 (19 kDa, Protein from Chlamydomonas reinhardtii)
Radial spoke 1 (Complex from Chlamydomonas reinhardtii)
Flagellar radial spoke protein 6 (48 kDa, Protein from Chlamydomonas reinhardtii)
Flagellar radial spoke protein 23 (61 kDa, Protein from Chlamydomonas reinhardtii)
Unknown protein (2 kDa, Protein from Chlamydomonas reinhardtii)
Flagellar radial spoke protein 11 (21 kDa, Protein from Chlamydomonas reinhardtii)
Phosphate ion (94 Da, Ligand)
Predicted protein (8 kDa, Protein from Chlamydomonas reinhardtii)
Cytochrome b5 heme-binding domain-containing protein (26 kDa, Protein from Chlamydomonas reinhardtii)
Flagellar radial spoke protein 3 (56 kDa, Protein from Chlamydomonas reinhardtii)
Flagellar radial spoke protein 10 (23 kDa, Protein from Chlamydomonas reinhardtii)
Flagellar radial spoke protein 9 (29 kDa, Protein from Chlamydomonas reinhardtii)
Flagellar radial spoke protein 1 (87 kDa, Protein from Chlamydomonas reinhardtii)
Flagellar radial spoke protein 7 (54 kDa, Protein from Chlamydomonas reinhardtii)
Flagellar radial spoke protein 14 (40 kDa, Protein from Chlamydomonas reinhardtii)
Flagellar radial spoke protein 16 (39 kDa, Protein from Chlamydomonas reinhardtii)

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EMDB [4]

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