CryoEM structure of Gq-coupled MRGPRD with a new agonist EP-2825
Resolution: 2.92 Å
EM Method: Single-particle
Fitted PDBs: 9dqh
Q-score: 0.123
Wang C, Liu Y, Lanier M, Yeager A, Singh I, Gumpper RH, Krumm BE, DeLeon C, Zhang S, Boehm M, Pittner R, Baron A, Dvorak L, Bacon C, Shoichet BK, Martinborough E, Fay JF, Cao C, Roth BL
Cell Rep (2024) 43 pp. 114942-114942 [ DOI: doi:10.1016/j.celrep.2024.114942 Pubmed: 39580805 ]
- Scfv16 (27 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Ep-2825-bound mrgprd-gq complex (137 kDa, Complex)
- Gs-mini-gq chimera (28 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Mas-related g-protein coupled receptor member d (36 kDa, Protein from Homo sapiens)
- 2-({1-[2-(4-chlorophenyl)-2-methylpropanoyl]piperidin-4-yl}amino)-5,6,7,8-tetrahydroquinazolin-4(3h)-one (428 Da, Ligand)
CryoEM structure of Gq-coupled MRGPRD with a new agonist EP-3945
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 9dqj
Q-score: 0.095
Wang C, Liu Y, Lanier M, Yeager A, Singh I, Gumpper RH, Krumm BE, DeLeon C, Zhang S, Boehm M, Pittner R, Baron A, Dvorak L, Bacon C, Shoichet BK, Martinborough E, Fay JF, Cao C, Roth BL
Cell Rep (2024) 43 pp. 114942-114942 [ DOI: doi:10.1016/j.celrep.2024.114942 Pubmed: 39580805 ]
- G(i)/g(s)/g(o) subunit gamma-2 (Complex from Homo sapiens)
- Scfv16 (27 kDa, Protein from Mus musculus)
- 2-({1-[1-(4-methoxyphenyl)cyclopropane-1-carbonyl]piperidin-4-yl}amino)quinazolin-4(3h)-one (418 Da, Ligand)
- Ep-3945-bound mrgprd-gq complex (137 kDa, Complex)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Scfv16 (Complex from Mus musculus)
- G(i) subunit alpha-2/g(s) subunit alpha isoforms xlas (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-2,guanine nucleotide-binding protein g(s) subunit alpha isoforms xlas (28 kDa, Protein from Homo sapiens)
- Mas-related g-protein coupled receptor member d (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Mas-related g-protein coupled receptor member d (36 kDa, Protein from Homo sapiens)
- G(i)/g(s)/g(t) subunit beta-1 (Complex from Homo sapiens)
CryoEM structure of fospropofol-bound MRGPRX4-Gq complex
Chien DC, Limjunyawong N, Cao C, Meixiong J, Peng Q, Ho CY, Fay JF, Roth BL, Dong X
Sci Transl Med (2024) 16 pp. eadk8198-eadk8198 [ Pubmed: 38718132 DOI: doi:10.1126/scitranslmed.adk8198 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Fospropofol-bound mrgprx4-gq complex (150 MDa, Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Soluble cytochrome b562,mas-related g-protein coupled receptor member x4 (53 kDa, Protein from Homo sapiens)
- Scfv16 (28 kDa, Protein from Mus musculus)
- [2,6-di(propan-2-yl)phenoxy]methyl dihydrogen phosphate (288 Da, Ligand)
- Gs-mini-gq chimera (28 kDa, Protein from Homo sapiens)
CryoEM structure of Gq-coupled MRGPRX1 with peptide agonist BAM8-22
Resolution: 2.87 Å
EM Method: Single-particle
Fitted PDBs: 8dwc
Q-score: 0.573
Liu Y, Cao C, Huang XP, Gumpper RH, Rachman MM, Shih SL, Krumm BE, Zhang S, Shoichet BK, Fay JF, Roth BL
Nat Chem Biol (2023) 19 pp. 416-422 [ Pubmed: 36302898 DOI: doi:10.1038/s41589-022-01173-6 ]
- Scfv16 (27 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Gs-mini-gq chimera (28 kDa, Protein from Homo sapiens)
- Proenkephalin-a (1 kDa, Protein from Bos taurus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Mrgprx1-gq bam8-22 (150 MDa, Complex from Homo sapiens)
- Mas-related g-protein coupled receptor member x1 (36 kDa, Protein from Homo sapiens)
Resolution: 2.71 Å
EM Method: Single-particle
Fitted PDBs: 8dwg
Q-score: 0.596
Liu Y, Cao C, Huang XP, Gumpper RH, Rachman MM, Shih SL, Krumm BE, Zhang S, Shoichet BK, Fay JF, Roth BL
Nat Chem Biol (2023) 19 pp. 416-422 [ Pubmed: 36302898 DOI: doi:10.1038/s41589-022-01173-6 ]
- Scfv16 (27 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Gs-mini-gq chimera (28 kDa, Protein from Homo sapiens)
- Proenkephalin-a (1 kDa, Protein from Bos taurus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- 2-[(cyclopropanesulfonyl)amino]-n-(2-ethoxyphenyl)benzamide (360 Da, Ligand)
- Mrgprx1-gq bam8-22 (150 MDa, Complex from Homo sapiens)
- Mas-related g-protein coupled receptor member x1 (36 kDa, Protein from Homo sapiens)
CryoEM structure of Gq-coupled MRGPRX1 with ligand Compound-16
Resolution: 3.25 Å
EM Method: Single-particle
Fitted PDBs: 8dwh
Q-score: 0.51
Liu Y, Cao C, Huang XP, Gumpper RH, Rachman MM, Shih SL, Krumm BE, Zhang S, Shoichet BK, Fay JF, Roth BL
Nat Chem Biol (2023) 19 pp. 416-422 [ Pubmed: 36302898 DOI: doi:10.1038/s41589-022-01173-6 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Gs-mini-gq chimera (28 kDa, Protein from Homo sapiens)
- N-{2-[(1-aminoisoquinolin-6-yl)oxy]-4-methylphenyl}-2-methoxybenzene-1-sulfonamide (435 Da, Ligand)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Mrgprx1-gq bam8-22 (150 MDa, Complex from Homo sapiens)
- Mas-related g-protein coupled receptor member x1 (36 kDa, Protein from Homo sapiens)
CryoEM structure of Gq-coupled MRGPRX2 with peptide agonist Cortistatin-14
Resolution: 2.45 Å
EM Method: Single-particle
Fitted PDBs: 7s8l
Q-score: 0.602
Cao C, Kang HJ, Singh I, Chen H, Zhang C, Ye W, Hayes BW, Liu J, Gumpper RH, Bender BJ, Slocum ST, Krumm BE, Lansu K, McCorvy JD, Kroeze WK, English JG, DiBerto JF, Olsen RHJ, Huang XP, Zhang S, Liu Y, Kim K, Karpiak J, Jan LY, Abraham SN, Jin J, Shoichet BK, Fay JF, Roth BL
Nature (2021) 600 pp. 170-175 [ Pubmed: 34789874 DOI: doi:10.1038/s41586-021-04126-6 ]
- Scfv16 (27 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Mrgprx2-gq cortistatin-14 (150 MDa, Complex from Homo sapiens)
- Mas-related g-protein coupled receptor member x2 (37 kDa, Protein from Homo sapiens)
- Cortistatin 14 (1 kDa, Protein from Homo sapiens)
- Gs-mini-gq chimera (28 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
CryoEM structure of Gi-coupled MRGPRX2 with peptide agonist Cortistatin-14
Resolution: 2.54 Å
EM Method: Single-particle
Fitted PDBs: 7s8m
Q-score: 0.562
Cao C, Kang HJ, Singh I, Chen H, Zhang C, Ye W, Hayes BW, Liu J, Gumpper RH, Bender BJ, Slocum ST, Krumm BE, Lansu K, McCorvy JD, Kroeze WK, English JG, DiBerto JF, Olsen RHJ, Huang XP, Zhang S, Liu Y, Kim K, Karpiak J, Jan LY, Abraham SN, Jin J, Shoichet BK, Fay JF, Roth BL
Nature (2021) 600 pp. 170-175 [ Pubmed: 34789874 DOI: doi:10.1038/s41586-021-04126-6 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Mrgprx2-gi1 cortistatin-14 (150 MDa, Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Cortistatin 14 (1 kDa, Protein from Homo sapiens)
- Scfv16 (28 kDa, Protein from Mus musculus)
- Soluble cytochrome b562, mas-related g-protein coupled receptor member x2 chimera (54 kDa, Protein from Homo sapiens)
CryoEM structure of Gq-coupled MRGPRX2 with small molecule agonist (R)-Zinc-3573
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7s8n
Q-score: 0.579
Cao C, Kang HJ, Singh I, Chen H, Zhang C, Ye W, Hayes BW, Liu J, Gumpper RH, Bender BJ, Slocum ST, Krumm BE, Lansu K, McCorvy JD, Kroeze WK, English JG, DiBerto JF, Olsen RHJ, Huang XP, Zhang S, Liu Y, Kim K, Karpiak J, Jan LY, Abraham SN, Jin J, Shoichet BK, Fay JF, Roth BL
Nature (2021) 600 pp. 170-175 [ Pubmed: 34789874 DOI: doi:10.1038/s41586-021-04126-6 ]
- Scfv16 (27 kDa, Protein from Mus musculus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Mas-related g-protein coupled receptor member x2 (37 kDa, Protein from Homo sapiens)
- Mrgprx2-gq (r)-zinc-3573 (150 MDa, Complex from Homo sapiens)
- Gs-mini-gq chimera (28 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- (3r)-n,n-dimethyl-1-[(8s)-5-phenylpyrazolo[1,5-a]pyrimidin-7-yl]pyrrolidin-3-amine (307 Da, Ligand)
CryoEM structure of Gi-coupled MRGPRX2 with small molecule agonist (R)-Zinc-3573
Resolution: 2.58 Å
EM Method: Single-particle
Fitted PDBs: 7s8o
Q-score: 0.591
Cao C, Kang HJ, Singh I, Chen H, Zhang C, Ye W, Hayes BW, Liu J, Gumpper RH, Bender BJ, Slocum ST, Krumm BE, Lansu K, McCorvy JD, Kroeze WK, English JG, DiBerto JF, Olsen RHJ, Huang XP, Zhang S, Liu Y, Kim K, Karpiak J, Jan LY, Abraham SN, Jin J, Shoichet BK, Fay JF, Roth BL
Nature (2021) 600 pp. 170-175 [ Pubmed: 34789874 DOI: doi:10.1038/s41586-021-04126-6 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Scfv16 (28 kDa, Protein from Mus musculus)
- Mrgprx2-gi1 (r)-zinc-3573 (150 MDa, Complex from Homo sapiens)
- Soluble cytochrome b562,mas-related g-protein coupled receptor member x2 chimera (54 kDa, Protein from Homo sapiens)
- (3r)-n,n-dimethyl-1-[(8s)-5-phenylpyrazolo[1,5-a]pyrimidin-7-yl]pyrrolidin-3-amine (307 Da, Ligand)